Researcher Profile and Settings

Researcher

• Name

  YOSIHARA Tositada

Profile and Settings

• Name

  Yoshihara, Toshitada

Foreign language

• Use in presentation

  English

• Use in publication

  English

Affiliation

• Gunma University, Associate Professor
• Gunma University, Associate Professor

Education

• 1998, 2001, Gunma University
• 1996, 1998, Gunma University
• 1993, 1996, Gunma University

Degree

• Doctor of Philosophy in Engineering

Research Experience

• 2002, 2004, National Institute of Advanced Industrial Science and Technology, Photoreaction Control Research Center
• 2007, 2014
• 2014, 9999, Gunma University, Graduate School of Science and Technology, Department of Materials and Bioscience
• 2001, 2002, Max-Planck-Institute for Biophysical Chemistry
• 2004, 2007

Research Activities

Research Areas

• Nanotechnology/Materials, Biochemistry
• Nanotechnology/Materials, Basic physical chemistry
• Life sciences, Biomedical engineering
• Life sciences, Biomaterials

Research Interests

• Photochemistry
• Bioimaging
• microspectroscopy

Published Papers

• Confocal microscopic oxygen imaging of xenograft tumors using Ir(III) complexes as in vivo intravascular and intracellular probes, T. Yoshihara, T. Tamura, S. Shiozaki, L.-C. Chou, R. Kakuchi, S. Rokudai, 08 Aug. 2024, Scientific Reports, 14, 18443
• りん光寿命顕微分光法による組織内酸素分圧イメージング, 吉原 利忠, 01 Mar. 2024, 144, 3, 275, 283, Scientific journal
• A silyl porphyrin derivative conjugated with 6-deoxy-6-sulfo-α-d-glucopyranose functions as an efficient photosensitizer for photodynamic therapy, Hiroaki Horiuchi, Kota Nishikawa, Nozomi Ishii, Koki Kano, Shunsuke Shinada, Nene Osawa, Aoi Horikoshi,Toshitada Yoshihara, Fumio Sugawara, Kengo Sakaguchi, Tetsuo Okustu, Shinji Katsura, Ichiro Matsuo, Masahiko Oshige, Feb. 2024, Photodiagnosis and Photodynamic Therapy, 45, 103898, Scientific journal
• An Optochemical Oxygen Scavenger Enabling Spatiotemporal Control of Hypoxia, Naoya Ieda, Masato Sawada, Runa Oguchi, Masato Itoh, Seina Hirakata, Daisuke Saitoh, Akito Nakao, Mitsuyasu Kawaguchi, Kazunobu Sawamoto, Toshitada Yoshihara, Yasuo Mori, Hidehiko Nakagawa, 12 Apr. 2023, Angewandte Chemie International Edition, 62, 20, e202217585, Scientific journal
• Blue-emitting lipid droplet probes based on coumarin dye for multi-color imaging of living cells and fatty livers of mice, Khulan Purevsuren, Yuhi Shibuta, Shuichi Shiozaki, Mina Tsunoda, Kiichi Mizukami, Seiji Tobita, Toshitada Yoshihara, Apr. 2023, Journal of Photochemistry and Photobiology A: Chemistry, 438, 114562, Scientific journal
• In Vivo Imaging of Lipid Droplets and Oxygen Status in Hepatic Tissues of Nonalcoholic Fatty Liver Model Mice Using a Lipophilic Ir(III) Complex, Khulan Purevsuren, Shuichi Shiozaki, Kiichi Mizukami, Seiji Tobita, Toshitada Yoshihara, 09 Feb. 2023, Analytical Chemistry, 95, 7, 3729, 3735, Scientific journal
• りん光寿命測定に基づく細胞・組織内酸素分圧, 吉原利忠, 26 Aug. 2022, 53, 2, 58, 65
• Determination of the physiological range of oxygen tension in bone marrow monocytes using two-photon phosphorescence lifetime imaging microscopy, Ayako Narazaki, Reito Shimizu, Toshitada Yoshihara, Junichi Kikuta, Reiko Sakaguchi, Seiji Tobita, Yasuo Mori, Masaru Ishii, Keizo Nishikawa, 10 Mar. 2022, Scientific Reports, 12, 3497, Scientific journal

  DOI
• Intracellular and Intravascular Oxygen Sensing of Pancreatic Tissues Based on Phosphorescence Lifetime Imaging Microscopy Using Lipophilic and Hydrophilic Iridium(III) Complexes, Toshitada Yoshihara, Nao Matsumura, Takuto Tamura, Shuichi Shiozaki, Seiji Tobita, 25 Feb. 2022, ACS Sensors, 7, 2, 545, 554, Scientific journal

  DOI
• Near-Infrared Emitting Ir(III) Complexes Bearing a Dipyrromethene Ligand for Oxygen Imaging of Deeper Tissues In Vivo, Kiichi Mizukami, Takako Muraoka, Shuichi Shiozaki, Seiji Tobita, Toshitada Yoshihara, 15 Feb. 2022, Analytical Chemistry, 94, 6, 2794, 2802, Scientific journal

  DOI
• Augmented Self‐Association by Electrostatic Forces in Thienopyrrole‐Fused Thiadiazoles that Contain an Ester instead of an Ether Linker, Yukako Naito, Ryo Moriguchi, Chitoshi Kitamura, Taisuke Matsumoto, Toshitada Yoshihara, Tsutomu Ishi-i, Yuka Nagata, Hiroki Takeshita, Kazunari Yoshizawa, Yoshihito Shiota, Kazumasa Suzuki, Shin-ichir, 14 Feb. 2022, Chemistry - An Asian Journal, 17, 4, e2021013, Scientific journal

  DOI
• Cyclometalated Iridium(III) Complex–Cationic Peptide Hybrids Trigger Paraptosis in Cancer Cells via an Intracellular Ca2+ Overload from the Endoplasmic Reticulum and a Decrease in Mitochondrial Membra, Chandrasekar Balachandran, Kenta Yokoi, Kana Naito, Jebiti Haribabu, Yuichi Tamura, Masakazu Umezawa, Koji Tsuchiya, Toshitada Yoshihara, Seiji Tobita, Shin Aoki, 21 Nov. 2021, Molecules, 26, 22, 7028, Scientific journal

  DOI
• Osteoclasts adapt to physioxia perturbation through DNA demethylation, Nishikawa, Keizo, Seno, Shigeto, Yoshihara, Toshitada, Narazaki, Ayako, Sugiura, Yuki, Shimizu, Reito, Kikuta, Junichi, Sakaguchi, Reiko, Suzuki, Norio, Takeda, Norihiko, Semba, Hi, 18 Oct. 2021, EMBO Reports, 22, e53035, Scientific journal

  DOI
• Ir(III)錯体のりん光寿命計測による細胞内酸素分圧測定法の開発, 吉原利忠, 28 Sep. 2021, 61, 5, 298, 302, Scientific journal

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• S,​C,​C- and O,​C,​C-​Bridged Triarylamines and Their Persistent Radical Cations, Shunpei Kataoka, Shuichi Suzuki, Yoshihito Shiota, Kazunari Yoshizawa, Taisuke Matsumoto, Motoko S. Asano, Toshitada Yoshihara, Chitoshi Kitamura, and Shin-ichiro Kato, 01 Sep. 2021, Journal of Organic Chemistry, 86, 18, 12559, 12568, Scientific journal

  DOI
• Membrane properties of amacrocyclic tetraether bisphosphatidylcholine lipid: Effect of a single membrane-​spanning polymethylene cross-​linkage between two head groups of ditetradecylphosphatidylcholi, Tsuchida, Naoyuki; Takagi, Toshiyuki; Takahashi, Hiroshi; Yoshihara, Toshitada; Tobita, Seiji; Sonoyama, Masashi, 01 May 2021, Biochimica et Biophysica Acta, Biomembranes, 1863, 183569
• Analysis of the triplet-​state kinetics of a photosensitizer for photoimmunotherapy by fluorescence correlation spectroscopy, Takakura, Hideo; Goto, Yuto; Kitamura, Akira; Yoshihara, Toshitada; Tobita, Seiji; Kinjo, Masataka; Ogawa, Mikako, 01 Mar. 2021, Journal of Photochemistry and Photobiology, A: Chemistry, 408, 113094
• Phosphorescent Ir(III) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging, Yasukagawa, Mami; Shimada, Aya; Shiozaki, Shuichi; Tobita, Seiji; Yoshihara, Toshitada, 26 Feb. 2021, Scientific Reports, 11, 4733
• A distinctive distribution of hypoxia-​inducible factor-​1α in cultured renal tubular cells with hypoperfusion simulated by coverslip placement, Honda, Tomoko; Hirakawa, Yosuke; Mizukami, Kiichi; Yoshihara, Toshitada; Tanaka, Tetsuhiro; Tobita, Seiji; Nangaku, Masaomi, 09 Jan. 2021, Physiological Reports, 9, e14689
• In vivo O2 imaging in hepatic tissues by phosphorescence lifetime imaging microscopy using Ir(III) complexes as intracellular probes, K. Mizukami; A. Katano; S. Shiozaki; T. Yoshihara; N. Goda; S. Tobita, Dec. 2020, Sci. Rep., 10, 21053, Scientific journal
• Spontaneously blinking fluorophores based on nucleophilic addition/dissociation of intracellular glutathione for live-cell super-resolution imaging., Akihiko Morozumi; Mako Kamiya; Shin-Nosuke Uno; Keitaro Umezawa; Ryosuke Kojima; Toshitada Yoshihara; Seiji Tobita; Yasuteru Urano, 28 Apr. 2020, Journal of the American Chemical Society, 142, 9625, 9633

  DOI
• Visualization of Lipid Droplets in Living Cells and Fatty Livers of Mice Based on the Fluorescence of π-Extended Coumarin Using Fluorescence Lifetime Imaging Microscopy, Yoshihara, Toshitada; Maruyama, Ryo; Shiozaki, Shuichi; Yamamoto, Koji; Kato, Shin-ichiro; Nakamura, Yosuke; Tobita, Seiji, 11 Mar. 2020, Analytical Chemistry, 92, 4996, 5003, Scientific journal

  DOI
• Synthesis, Structures, and Properties of Neutral and Radical Cationic S,C,C-Bridged Triphenylamines, Shin-ichiro Kato; Takanori Matsuoka; Shuichi Suzuki; Motoko S. Asano; Toshitada Yoshihara; Seiji Tobita; Taisuke Matsumoto; Chitoshi Kitamura, 17 Jan. 2020, Organic Letters, 22, 734, 738, Scientific journal

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• Absolute quantum yield measurements of near-infrared emission with correction for solvent absorption, R. Hoshi; K. Suzuki; N. Hasebe; T. Yoshihara; S. Tobita, 2020, Anal. Chem., 92, 607, 611, Scientific journal
• Chemical transformation of push-pull fluorenones: push-pull dibenzodicyanofulvenes as well as fluorenone- and dibenzodicyanofulvene-tetracyanobutadiene conjugates, S. Kato; T. Kijima; Y. Shiota; T. Abe; S. Kuwako; H. Miyauchi; N. Yoshikawa; K. Yamamoto; K. Yoshizawa; T. Yoshihara; S. Tobita; Y. Nakamura, 2020, Org. Bio. Chem., 18, 4198, 4209, Scientific journal
• Design of spontaneously blinking fluorophore for live-cell super resolution imaging based on quantum-chemical calculations, T. Ryo; M. Kamiya; A. Morozumi; Y. Miyazaki; H. Fujioka; A. Nanjo; R. Kojima; T. Komatsu; T. Ueno; K. Hanaoka; T. Yoshihara; S. Tobita; Y. Urano, 2020, Chem. Commun., 56, 13173, 13176, Scientific journal
• Fluorescence Quenching-based Assay for Measuring Golgi endo-α-Mannosidase., Kanae Sano; Taiki Kuribara; Nozomi Ishii; Ayumi Kuroiwa; Toshitada Yoshihara; Seiji Tobita; Kiichiro Totani; Ichiro Matsuo, 03 Jun. 2019, Chemistry, an Asian journal, 14, 11, 1965, 1969

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• Ratiometric oxygen probes with a cell-penetrating peptide for imaging oxygen levels in living cells, Mami Yasukagawa; Keiich Yamada; Seiji Tobita; ToshitadaYoshihara, 2019, Journal of Photochemistry and Photobiology A: Chemistry, 383, 111983
• Structure-efficiency relationship of photoinduced electron transfer-triggered nitric oxide releasers, Naoya Ieda; Yumina Oka; Toshitada Yoshihara; Seiji Tobita; Takahiro Sasamori; Mitsuyasu Kawaguchi; Hidehiko Nakagawa, 2019, Scientific Reports, 9, 1430
• Intravital phosphorescence lifetime imaging of the renal cortex accurately measures renal hypoxia, Yosuke Hirakawa; Kiichi Mizukami; Toshitada Yoshihara; Ippei Takahashi; Purevsuren Khulan; Tomoko Honda; Imari Mimura; Tetsuhiro Tanaka; Seiji Tobita; Masaomi Nangaku, 01 Jun. 2018, Kidney International, 93, 6, 1483, 1489, Scientific journal

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• Ir(iii) complex-based oxygen imaging of living cells and ocular fundus with a gated ICCD camera, H. Akiyama; I. Takahashi; Y. Shimoda; R. Mukai; T. Yoshihara; S. Tobita, 2018, Photochemical and Photobiological Sciences, 17, 6, 846, 853, Scientific journal

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• Extension of pi-system of silylated porphyrin derivative for photodynamic therapy, Hiroaki Horiuchi; Asumi Sakai; Shingo Akiyama; Remi Ikeda; Satoshi Ito; Masaru Furuya; Yuta Gomibuchi; Muneki Ichikawa; Toshitada Yoshihara; Seiji Tobita; Tetsuo Okutsu, Apr. 2017, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 339, 19, 24, Scientific journal

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• Oxygen imaging of living cells and tissues using luminescent molecular probes, Toshitada Yoshihara; Yosuke Hirakawa; Masahiro Hosaka; Masaomi Nangaku; Seiji Tobita, Mar. 2017, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY C-PHOTOCHEMISTRY REVIEWS, 30, 71, 95

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• Interrogation of metabolic and oxygen states of tumors with fiber-based luminescence lifetime spectroscopy, Maria Lukina; Anna Orlova; Marina Shirmanova; Daniil Shirokov; Anton Pavlikov; Antje Neubauer; Hauke Studier; Wolfgang Becker; Elena Zagaynova; Toshitada Yoshihara; Seiji Tobita; Vladislav Shcheslavskiy, Feb. 2017, OPTICS LETTERS, 42, 4, 731, 734, Scientific journal

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• Push-pull fluorenones and benzazulenequinones: regioselective [4+2] and [2+2] cycloadditions of benzopentalenequinone derivative and alkynes bearing an aniline moiety, Shin-ichiro Kato; Tomokazu Kijima; Yoshihito Shiota; Toshitada Yoshihara; Seiji Tobita; Kazunari Yoshizawa; Yosuke Nakamura, Oct. 2016, TETRAHEDRON LETTERS, 57, 41, 4604, 4607, Scientific journal

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• Biological oxygen sensing via two-photon absorption by an Ir(III) complex using a femtosecond fiber laser, Hiroki Moritomo; Akinari Fujii; Yasutaka Suzuki; Toshitada Yoshihara; Seiji Tobita; Jun Kawamata, Sep. 2016, JAPANESE JOURNAL OF APPLIED PHYSICS, 55, 9, 92401, Scientific journal

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• Intracellular and in vivo oxygen sensing using phosphorescent iridium(III) complexes, Seiji Tobita; Toshitada Yoshihara, Aug. 2016, CURRENT OPINION IN CHEMICAL BIOLOGY, 33, 39, 45

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• Phosphorescence quenching of neutral and cationic iridium(III) complexes by molecular oxygen and aromatic electron acceptors, Naoya Hasebe; Yonekazu Deguchi; Saori Murayama; Toshitada Yoshihara; Hiroaki Horiuchi; Tetsuo Okutsu; Seiji Tobita, Jun. 2016, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 324, 134, 144, Scientific journal

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• The effect of central metal on the photodynamic properties of silylated tetraphenylporphyrin derivative, Hiroaki Horiuchi; Keisuke Terashima; Asumi Sakai; Daisuke Suda; Toshitada Yoshihara; Atsushi Kobayashi; Seiji Tobita; Tetsuo Okutsu, May 2016, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 321, 72, 78, Scientific journal

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• A series of π-extended thiadiazoles fused with electron-donating heteroaromatic moieties: Synthesis, properties, and polymorphic crystals, Shin-Ichiro Kato; Takayuki Furuya; Masashi Nitani; Naoya Hasebe; Yutaka Ie; Yoshio Aso; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura, 22 Dec. 2015, Chemistry - A European Journal, 21, 7, 3115, 3128, Scientific journal

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• Quantitating intracellular oxygen tension in vivo by phosphorescence lifetime measurement, Yosuke Hirakawa; Toshitada Yoshihara; Mako Kamiya; Imari Mimura; Daichi Fujikura; Tsuyoshi Masuda; Ryohei Kikuchi; Ippei Takahashi; Yasuteru Urano; Seiji Tobita; Masaomi Nangaku, Dec. 2015, SCIENTIFIC REPORTS, 5, 17838, Scientific journal

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• Ratiometric molecular probes based on dual emission of a blue fluorescent coumarin and a red phosphorescent cationic iridium(III) complex for intracellular oxygen sensing, Toshitada Yoshihara; Saori Murayama; Seiji Tobita, 09 Jun. 2015, Sensors (Switzerland), 15, 6, 13503, 13521, Scientific journal

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• Intracellular and in Vivo Oxygen Sensing Using Phosphorescent Ir(III) Complexes with a Modified Acetylacetonato Ligand, Toshitada Yoshihara; Masahiro Hosaka; Motoki Terata; Kazuki Ichikawa; Saori Murayama; Asami Tanaka; Masanobu Mori; Hideyuki Itabashi; Toshiyuki Takeuchi; Seiji Tobita, Mar. 2015, ANALYTICAL CHEMISTRY, 87, 5, 2710, 2717, Scientific journal

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• Absolute Phosphorescence Quantum Yields of Singlet Molecular Oxygen in Solution Determined Using an Integrating Sphere Instrument, Naoya Hasebe; Kengo Suzuki; Hiroaki Horiuchi; Hiromi Suzuki; Toshitada Yoshihara; Tetsuo Okutsu; Seiji Tobita, Feb. 2015, ANALYTICAL CHEMISTRY, 87, 4, 2360, 2366, Scientific journal

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• Mitochondria-targeted oxygen probes based on cationic iridium complexes with a 5-amino-1, 10-phenanthroline ligand, Toshitada Yoshihara; Saori Murayarria; Tsuyoshi Masuda; Toshiki Kikuchi; Kohei Yoshida; Masahiro Hosaka; Seiji Tobita, Feb. 2015, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 299, 172, 182, Scientific journal

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• Amphiphilic benzothiadiazole-triphenylamine-based aggregates that emit red light in water, Tsutomu Ishi-i; Ikumi Kitahara; Shimpei Yamada; Yusuke Sanada; Kazuo Sakurai; Asami Tanaka; Naoya Hasebe; Toshitada Yoshihara; Seiji Tobita, 2015, ORGANIC & BIOMOLECULAR CHEMISTRY, 13, 6, 1818, 1828, Scientific journal

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• Minimal Thioflavin T Modifications Improve Visual Discrimination of Guanine-Quadruplex Topologies and Alter Compound-Induced Topological Structures, Yuka Kataoka; Hiroto Fujita; Yuuya Kasahara; Toshitada Yoshihara; Seiji Tobita; Masayasu Kuwahara, Dec. 2014, ANALYTICAL CHEMISTRY, 86, 24, 12078, 12084, Scientific journal

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• A spontaneously blinking fluorophore based on intramolecular spirocyclization for live-cell super-resolution imaging., Shin-Nosuke Uno; Mako Kamiya; Toshitada Yoshihara; Ko Sugawara; Kohki Okabe; Mehmet C Tarhan; Hiroyuki Fujita; Takashi Funatsu; Yasushi Okada; Seiji Tobita; Yasuteru Urano, Aug. 2014, Nature chemistry, 6, 8, 681, 9

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• Mitochondrial Respiratory Function Induces Endogenous Hypoxia, Sara Prior; Ara Kim; Toshitada Yoshihara; Seiji Tobita; Toshiyuki Takeuchi; Masahiro Higuchi, Feb. 2014, PLOS ONE, 9, 2, e88911, Scientific journal

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• Systematic Structure-Property Investigations on a Series of Alternating Carbazole-Thiophene Oligomers, Shin-ichiro Kato; Satoru Shimizu; Atsushi Kobayashi; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura, Jan. 2014, JOURNAL OF ORGANIC CHEMISTRY, 79, 2, 618, 629, Scientific journal

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• Tetraalkoxyphenanthrene-fused dehydroannulenes: Synthesis, self-assembly, and electronic, optical, and electrochemical properties, Shin-Ichiro Kato; Nobutaka Takahashi; Hidekazu Tanaka; Atsushi Kobayashi; Toshitada Yoshihara; Seiji Tobita; Takeshi Yamanobe; Hiroki Uehara; Yosuke Nakamura, 02 Sep. 2013, Chemistry - A European Journal, 19, 36, 12138, 12151, Scientific journal

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• Fluorescent Peptides Labeled with Environment-Sensitive 7-Aminocoumarins and Their Interactions with Lipid Bilayer Membranes and Living Cells, Tokiko Murase; Toshitada Yoshihara; Keiichi Yamada; Seiji Tobita, Apr. 2013, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 86, 4, 510, 519, Scientific journal

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• Bicarbazoles: Systematic Structure-Property Investigations on a Series of Conjugated Carbazole Dimers, Shin-ichiro Kato; Hiroto Noguchi; Atsushi Kobayashi; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura, Oct. 2012, JOURNAL OF ORGANIC CHEMISTRY, 77, 20, 9120, 9133, Scientific journal

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• pi-Extended Thiadiazoles Fused with Thienopyrrole or Indole Moieties: Synthesis, Structures, and Properties, Shin-ichiro Kato; Takayuki Furuya; Atsushi Kobayashi; Masashi Nitani; Yutaka Ie; Yoshio Aso; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura, Sep. 2012, JOURNAL OF ORGANIC CHEMISTRY, 77, 17, 7595, 7606, Scientific journal

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• Environment-Sensitive Fluorophores with Benzothiadiazole and Benzoselenadiazole Structures as Candidate Components of a Fluorescent Polymeric Thermometer, Seiichi Uchiyama; Kohki Kimura; Chie Gota; Kohki Okabe; Kyoko Kawamoto; Noriko Inada; Toshitada Yoshihara; Seiji Tobita, Jul. 2012, CHEMISTRY-A EUROPEAN JOURNAL, 18, 31, 9552, 9563

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• Mitochondria-specific Oxygen Probe Based on Iridium Complexes Bearing Triphenylphosphonium Cation, Tokiko Murase; Toshitada Yoshihara; Seiji Tobita, Mar. 2012, CHEMISTRY LETTERS, 41, 3, 262, 263, Scientific journal

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• Remarkable fluorescence enhancement of benzo[g]chromen-2-ones induced by hydrogen-bonding interactions with protic solvents, Atsushi Kobayashi; Kazuyuki Takehira; Toshitada Yoshihara; Seiichi Uchiyama; Seiji Tobita, 2012, PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 11, 8, 1368, 1376, Scientific journal

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• Ratiometric Molecular Sensor for Monitoring Oxygen Levels in Living Cells, Toshitada Yoshihara; Yuji Yamaguchi; Masahiro Hosaka; Toshiyuki Takeuchi; Seiji Tobita, 2012, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 51, 17, 4148, 4151, Scientific journal

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• Control of Photophysical Properties of 1,8-Naphthalimides by Electron-Withdrawing Substituents Introduced into N-Alkyl Side Chains, Kazuhiko Matsubayashi; Chisato Kajimura; Yasuo Kubo; Toshitada Yoshihara; Seiji Tobita, May 2011, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 84, 5, 520, 525, Scientific journal

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• Kinetic Analysis for the Effect of Intramolecular Hydrogen Bonding on Photophysical Properties of N-Hydroxyalkyl-1,8-naphthalimides, Kazuhiko Matsubayashi; Chisato Kajimura; Hideo Shiratori; Yasuo Kubo; Toshitada Yoshihara; Seiji Tobita, Sep. 2010, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 83, 9, 1067, 1073, Scientific journal

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• Phosphorescent Light-Emitting Iridium Complexes Serve as a Hypoxia-Sensing Probe for Tumor Imaging in Living Animals, Shaojuan Zhang; Masahiro Hosaka; Toshitada Yoshihara; Kazuya Negishi; Yasuhiko Iida; Seiji Tobita; Toshiyuki Takeuchi, Jun. 2010, CANCER RESEARCH, 70, 11, 4490, 4498, Scientific journal

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• Absolute Measurements of Photoluminescence Quantum Yields of 1-Halonaphthalenes in 77K Rigid Solution Using an Integrating Sphere Instrument, Atsushi Kobayashi; Kengo Suzuki; Toshitada Yoshihara; Seiji Tobita, Mar. 2010, CHEMISTRY LETTERS, 39, 3, 282, 283, Scientific journal

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• Synthesis and Photophysical Properties of N-Arylcarbazolophanes, Hideyuki Shimizu; Yasuhiko Kakinoya; Kazuyuki Takehira; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura; Jun Nishimura, Jul. 2009, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 82, 7, 860, 869, Scientific journal

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• SUBSTITUENT EFFECTS ON PHOTOPHYSICAL PROPERTIES OF N-THIENYLCARBAZOLES, Hideyuki Shimizu; Atsushi Kobayashi; Shingo Itoi; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura; Jun Nishimura, May 2009, HETEROCYCLES, 78, 5, 1265, 1269, Scientific journal

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• The Rate of Excited-state Proton Transfer to Solvent from Trifluoromethylphenols in Water, Shigeo Kaneko; Toshitada Yoshihara; Seiji Tobita, Apr. 2009, CHEMISTRY LETTERS, 38, 4, 312, 313, Scientific journal

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• Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector, Kengo Suzuki; Atsushi Kobayashi; Shigeo Kaneko; Kazuyuki Takehira; Toshitada Yoshihara; Hitoshi Ishida; Yoshimi Shiina; Shigero Oishic; Seiji Tobita, 2009, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 11, 42, 9850, 9860, Scientific journal

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• Measurement of photoluminescence efficiency of Ir(III) phenylpyridine derivatives in solution and solid-state films, Ayataka Endo; Kengo Suzuki; Toshitada Yoshihara; Seiji Tobita; Masayuki Yahiro; Chihaya Adachi, Jul. 2008, CHEMICAL PHYSICS LETTERS, 460, 1-3, 155, 157, Scientific journal

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• Temperature-dependent fluorescence lifetime of a fluorescent polymeric thermometer, Poly(N-isopropylacrylamide), labeled by polarity and hydrogen bonding sensitive 4-sulfamoyl-7-aminobenzofurazan, Chie Gota; Seiichi Uchiyama; Toshitada Yoshihara; Seiji Tobita; Tomohiko Ohwada, Mar. 2008, JOURNAL OF PHYSICAL CHEMISTRY B, 112, 10, 2829, 2836, Scientific journal

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• Photoinduced intramolecular electron transfer of carbazole trimer-[60]fullerene studied by laser flash photolysis techniques, Takashi Konno; Mohamed E. El-Khouly; Yosuke Nakamura; Keisuke Kinoshita; Yasuyuki Araki; Osamu Ito; Toshitada Yoshihara; Seiji Tobita; Jun Nishimura, Jan. 2008, JOURNAL OF PHYSICAL CHEMISTRY C, 112, 4, 1244, 1249, Scientific journal

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• Extremely low-threshold amplified spontaneous emission of 9,9 '-spirobifluorene derivatives and electroluminescence from field-effect transistor structure, Hajime Nakanotani; Seiji Akiyama; Dai Ohnishi; Masato Moriwake; Masayuki Yahiro; Toshitada Yoshihara; Seiji Tobita; Chihaya Adachi, Sep. 2007, ADVANCED FUNCTIONAL MATERIALS, 17, 14, 2328, 2335, Scientific journal

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• Effect of pH on absorption spectra of photogenerated holes in nanocrystalline TiO2 films, Toshitada Yoshihara; Yoshiaki Tamaki; Akihiro Furube; Miki Murai; Kohjiro Hara; Ryuzi Katoh, Apr. 2007, CHEMICAL PHYSICS LETTERS, 438, 4-6, 268, 273, Scientific journal

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• Synthesis and photophysical properties of [60]fullerene adducts carrying oligocarbazole dendrons, Yosuke Nakamura; Takashi Konno; Satoru Watanabe; Masato Suzuki; Toshitada Yoshihara; Seiji Tobita; Jun Nishimura, Feb. 2007, CHEMISTRY LETTERS, 36, 2, 264, 265, Scientific journal

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• Environment-sensitive fluorophore emitting in protic environments, Seiichi Uchiyama; Kazuyuki Takehira; Toshitada Yoshihara; Seiji Tobita; Tomohiko Ohwada, Dec. 2006, ORGANIC LETTERS, 8, 25, 5869, 5872, Scientific journal

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• Intramolecular charge transfer and dielectric solvent relaxation in n-propyl cyanide N-phenylpyrrole and 4-Dimethylamino-4 '-cyanostilbene, Sergey I. Druzhinin; Victor A. Galievsky; Toshitada Yoshihara; Klaas A. Zachariasse, Nov. 2006, JOURNAL OF PHYSICAL CHEMISTRY A, 110, 47, 12760, 12768, Scientific journal

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• Photophysical properties of 3-[2-cyano-4-(dimethylamino)phenyl]alanine: A highly fluorescent and environment-sensitive amino acid with small molecular size, Juro Oshima; Hitomi Tsujimoto; Toshitada Yoshihara; Keiichi Yamada; Ryoichi Katakai; Seiji Tobita, Jun. 2006, CHEMISTRY LETTERS, 35, 6, 620, 621, Scientific journal

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• Water-induced fluorescence quenching of mono- and dicyanoanilines, J Oshima; T Yoshihara; S Tobita, Jun. 2006, CHEMICAL PHYSICS LETTERS, 423, 4-6, 306, 311, Scientific journal

  DOI
• Extreme fluorescence sensitivity of some aniline derivatives to aqueous and nonaqueous environments: Mechanistic study and its implication as a fluorescent probe, J Oshima; S Shiobara; H Naoumi; S Kaneko; T Yoshihara; AK Mishra; S Tobita, Apr. 2006, JOURNAL OF PHYSICAL CHEMISTRY A, 110, 14, 4629, 4637, Scientific journal

  DOI
• An efficient energy transfer found in triphenylene/hexaazatriphenylene system with electronic complimentarity and structural similarity, T Ishi-i; H Tashiro; R Kuwahara; S Mataka; T Yoshihara; S Tobita, Feb. 2006, CHEMISTRY LETTERS, 35, 2, 158, 159, Scientific journal

  DOI
• Synthesis and complexing properties of [2.n](2,6)pyridinocrownophanes, S Inokuma; H Ide; T Yonekura; T Funaki; S Kondo; S Shiobara; T Yoshihara; S Tobita; J Nishimura, Mar. 2005, JOURNAL OF ORGANIC CHEMISTRY, 70, 5, 1698, 1703, Scientific journal

  DOI
• Kinetics of intramolecular charge transfer with N-phenylpyrrole in alkyl cyanides, T Yoshihara; SI Druzhinin; A Demeter; N Kocher; D Stalke; KA Zachariasse, Mar. 2005, JOURNAL OF PHYSICAL CHEMISTRY A, 109, 8, 1497, 1509, Scientific journal

  DOI
• Novel conjugated organic dyes for efficient dye-sensitized solar cells, K Hara; T Sato; R Katoh; A Furube; T Yoshihara; M Murai; M Kurashige; S Ito; A Shinpo; S Suga; H Arakawa, Feb. 2005, ADVANCED FUNCTIONAL MATERIALS, 15, 2, 246, 252, Scientific journal

  DOI
• The exo-outstretching effect governing the exclusive stereoselectivity of the intramolecular [2+2] photocycloaddition of vinylarenes, J Nishimura; T Funaki; N Saito; S Inokuma; Y Nakamura; S Tajima; T Yoshihara; S Tobita, 2005, HELVETICA CHIMICA ACTA, 88, 6, 1226, 1239, Scientific journal

  DOI
• Intramolecular and intermolecular hydrogen-bonding effects on photophysical properties of 2 '-aminoacetophenone and its derivatives in solution, H Shimada; A Nakamura; T Yoshihara; S Tobita, 2005, PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 4, 4, 367, 375, Scientific journal

  DOI
• Ultrafast direct and indirect electron-injection processes in a photoexcited dye-sensitized nanocrystalline zinc oxide film: The importance of exciplex intermediates at the surface, A Furube; R Katoh; T Yoshihara; K Hara; S Murata; H Arakawa; M Tachiya, Aug. 2004, JOURNAL OF PHYSICAL CHEMISTRY B, 108, 33, 12583, 12592, Scientific journal

  DOI
• Trace analysis by transient absorption spectroscopy: estimation of the solubility of C-60 in polar solvents, T Yoshihara; M Murai; Y Tamaki; A Furube; R Katoh, Aug. 2004, CHEMICAL PHYSICS LETTERS, 394, 1-3, 161, 164, Scientific journal

  DOI
• Microscopic imaging of the efficiency of electron injection from excited sensitizer dye into nanocrystalline ZnO film, R Katoh; A Furube; Y Tamaki; T Yoshihara; M Murai; K Hara; S Murata; H Arakawa; M Tachiya, Aug. 2004, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 166, 1-3, 69, 74, Scientific journal

  DOI
• Fast intramolecular charge transfer with a planar rigidized electron donor/acceptor molecule, T Yoshihara; SI Druzhinin; KA Zachariasse, Jul. 2004, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 126, 27, 8535, 8539, Scientific journal

  DOI
• Efficiencies of electron injection from excited N3 dye into nanocrystalline semiconductor (ZrO2, TiO2, ZnO, Nb2O5, SnO2, In2O3) films, R Katoh; A Furube; T Yoshihara; K Hara; G Fujihashi; S Takano; S Murata; H Arakawa; M Tachiya, Apr. 2004, JOURNAL OF PHYSICAL CHEMISTRY B, 108, 15, 4818, 4822, Scientific journal

  DOI
• A new-type photoreaction of a carbonyl compound - Part 1. Photoinduced ω-bond dissociation in p-mercaptomethylbenzophenone studied by time-resolved EPR technique, steady-state and flaser flash photolyses in solution, Minoru Yamaji; Toshitada Yoshihara; Takashi Tachikawa; Shozo Tero-Kubota; Seiji Tobita; Haruo Shizuka; Bronislaw Marciniak, 15 Mar. 2004, Journal of Photochemistry and Photobiology A: Chemistry, 162, 2-3, 513, 520, Scientific journal

  DOI
• Identification of reactive species in photoexcited nanocrystalline TiO2 films by wide-wavelength-range (400-2500 nm) transient absorption spectroscopy, T Yoshihara; R Katoh; A Furube; Y Tamaki; M Murai; K Hara; S Murata; H Arakawa; M Tachiya, Mar. 2004, JOURNAL OF PHYSICAL CHEMISTRY B, 108, 12, 3817, 3823, Scientific journal

  DOI
• Quantitative estimation of the efficiency of electron injection from excited sensitizer dye into nanocrystalline ZnO film, T Yoshihara; R Katoh; A Furube; M Murai; Y Tamaki; K Hara; S Murata; H Arakawa; M Tachiya, Feb. 2004, JOURNAL OF PHYSICAL CHEMISTRY B, 108, 8, 2643, 2647, Scientific journal

  DOI
• Thermally activated internal conversion with 4-(dimethylamino)benzonitrile, 4-(methylamino)benzonitrile, and 4-aminobenzonitrile in alkane solvents. No correlation with intramolecular charge transfer, SI Druzhinin; A Demeter; VA Galievsky; T Yoshihara; KA Zachariasse, Oct. 2003, JOURNAL OF PHYSICAL CHEMISTRY A, 107, 40, 8075, 8085, Scientific journal

  DOI
• Singlet excited state dipole moments of dual fluorescent N-phenylpyrroles and 4-(dimethylamino)benzonitrile from solvatochromic and thermochromic spectral shifts, T Yoshihara; VA Galievsky; SI Druzhinin; S Saha; KA Zachariasse, Mar. 2003, PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2, 3, 342, 353, Scientific journal

  DOI
• Picosecond and nanosecond fluorescence decays of 4-(dimethylamino)phenylacetylene in comparison with those of 4-(dimethylamino)benzonitrile. No evidence for intramolecular charge transfer and a nonfluorescing intramolecular charge-transfer state, KA Zachariasse; T Yoshihara; SI Druzhinin, Jul. 2002, JOURNAL OF PHYSICAL CHEMISTRY A, 106, 26, 6325, 6333, Scientific journal

  DOI
• Photochemistry of 1,4-anthraquinone studied by steady-state and laser-flash photolysis, T Yoshihara; M Yamaji; T Itoh; J Nishimura; H Shizuka; S Tobita, Apr. 2001, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 140, 1, 7, 13, Scientific journal

  DOI
• Internal conversion of o-aminoacetophenone in solution, T Yoshihara; H Shimada; H Shizuka; S Tobita, 2001, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 3, 22, 4972, 4978, Scientific journal

  DOI
• Radiationless processes of N,N-dimethylaniline derivatives in solution. Time-resolved optoacoustic studies., S Tobita; R Kamiyama; K Takehira; T Yoshihara; S Yotoriyama; H Shizuka, 2001, ANALYTICAL SCIENCES, 17, S50, S52, Scientific journal

  DOI
• Hydrogen atom transfer and electron transfer reactions in the triplet π,π* state of 1,4-anthraquinone studied by CIDEP techniques and laser flash photolysis, Toshitada Yoshihara; Minoru Yamaji; Takao Itoh; Haruo Shizuka; Takuji Shimokage; Shozo Tero-Kubota, 01 Mar. 2000, Physical Chemistry Chemical Physics, 2, 5, 993, 1000, Scientific journal

  DOI
• Laser photolysis studies on hydrogen atom abstraction by triplet benzophenone from hydroxyanilinium ions, Toshitada Yoshihara; Minoru Yamaji; Haruo Shizuka, 25 Oct. 1996, Chemical Physics Letters, 261, 4-5, 431, 436, Scientific journal

  DOI

MISC

• りん光寿命法による細胞・組織内酸素イメージング, 水上輝市、吉原利忠, 20 Jun. 2022, 54, 7, 16（368）, 19（371）
• りん光性分子プローブによる生体内酸素環境のイメージング, 本田智子, 平川陽亮, 吉原利忠, 飛田成史, 南学正臣, Apr. 2018
• In-vivo phosphorescence imaging of cancer using iridium complexes, Toshitada Yoshihara; Yasuyuki Karasawa; Shaojuan Zhang; Masahiro Hosaka; Toshiyuki Takeuchi; Yasuhiko Iida; Keigo Endo; Takashi Imamura; Seiji Tobita, 2009, REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS, 7190, 71900X-1-71900X-8

  DOI
• Iridium complex probes for monitoring of cellular oxygen levels and imaging of hypoxic tissues, Toshitada Yoshihara; Atsushi Kobayashi; Shinpei Oda; Masahiro Hosaka; Toshiyuki Takeuchi; Seiji Tobita, 2012, REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS IV, 8233, 82330A-1-82330A-8

  DOI
• Cyclic b-Sheet Antimicrobial Peptides with Enviroment-sensitive Fluorophores: Photophyscial Properties and Molecular Orientation in Lipid Bilayers,, K. Yamada; T. Yoshihara; T. Katsu; H. Oku; S. Tobita, 2009, Peptide Science, 45, 281
• Ex vivo and in vivo behavior of red and near infrared-emitting iridium(III) complexes, T. Yoshihara; Y. Ohori; Y. Karasawa; M. Hosaka; T. Takeuchi; S. Tobita, 2010, Photomedicine and Photobiology, 32, 39, 40
• Synthesis and biological evaluation of fluorescent analogs of polymyxin B, H. Suda; J. Nozaki; T. Yoshihara; K. Komagoe; H. Oku; S. Tobita; T. Katsu; K. Yamada, 2011, Peptide Science, 48, 97, 100
• 紫外線吸収剤の理論と新規物質開発の考え方, 飛田成史; 吉原利忠; 小林敦; 島田博文, 2007, 7, 9, 363

  DOI
• Photophysical study of iridium complexes by absolute photoluminescence quantum yield measurements using an integrating sphere, Kengo Suzuki; Ayataka Endo; Toshitada Yoshihara; Seiji Tobita; Masayuki Yahiro; Daisuke Yokoyama; Chihaya Adachi, 2009, Proceedings of SPIE - The International Society for Optical Engineering, 7415, 741504/1-741504/8

  DOI
• Development of Photodetection Method of Tumor Using Phosphorescence of Iridium(III) Complexes, T. Yoshihara; Y. Karasawa; K. Negishi; M. Fushiya; M. Hosaka; T. Takeuchi; S. Tobita, 2009, 31, 13
• イリジウム錯体の‘りん光’を利用した癌のin vivo発光イメージング, 吉原利忠; 穂坂正博; 竹内利行; 飛田成史, 2010, 3, 26
• イリジウム錯体のりん光を利用した酸素濃度センサー, 飛田成史; 吉原利忠; 穂坂正博; 竹内利行, 2010, 55, 584
• Cyclic b-Sheet Antimicrobial Peptides with Enviroment-sensitive Fluorophores: Photophyscial Properties and Molecular Orientation in Lipid Bilayers,, K. Yamada; T. Yoshihara; T. Katsu; H. Oku; S. Tobita, 2009, 45, 281
• りん光分子プローブを用いた癌環境イメージング-低酸素の可視化-, 飛田成史; 吉原利忠; 穂坂正博; 竹内利行, 2009, 35, 538
• Synthsis of environment-sensitive fluorescent phenylalanine derivatives for investigating peptide-lipid membrane interaction, K. Yamada; T. Yoshihara; K. Yokoo; J. Oshima; K. Komagoe; H. Oku; T. Kastu; S. Tobita; I. Matsuo, 2010, 46, 127, 130
• Syntheses of fluorescent peptides labeled with 7-dimethylaminocoumarin and their photophysical properties in liposome membranes, T. Murase; T. Yoshihara; K. Yamada; S. Tobita, 2010, 47, 268
• Ex vivo and in vivo behavior of red and near infrared-emitting iridium(III) complexes, T. Yoshihara; Y. Ohori; Y. Karasawa; M. Hosaka; T. Takeuchi; S. Tobita, 2010, 32, 39, 40
• Development of iridium(III) complexes with high cellular uptake efficiency, T. Yoshihara; K. Ichikawa; M. Hosaka; T. Takeuchi; S. Tobita, 2011, 33, 15, 16
• Synthesis and biological evaluation of fluorescent analogs of polymyxin B, H. Suda; J. Nozaki; T. Yoshihara; K. Komagoe; H. Oku; S. Tobita; T. Katsu; K. Yamada, 2011, 48, 97, 100
• りん光プローブの設計・開発に基づくin vivo低酸素環境イメージング, 飛田成史; 吉原利忠; 穂坂正博; 竹内利行, 2012, 30, 1100, 1106
• りん光寿命計測による細胞・組織内酸素濃度定量, 吉原 利忠; 飛田 成史, 2017, 53, 3, 220, 224

  DOI
• りん光性分子プローブによる低酸素細胞イメージング, 吉原 利忠; 村瀬 秋子; 飛田 成史, 2013, 45, 9, 431, 434
• 光学的方法による細胞・組織内酸素計測, 飛田 成史; 吉原 利忠, 2013, 45, 12, 592, 595
• イリジウム錯体のりん光を用いた低酸素細胞・組織イメージング, 吉原 利忠; 飛田 成史, 2013, 44, 2, 86, 89
• Ratiometric small molecular probes based on dual emission for intracellular oxygen sensing, Toshitada Yoshihara; Seiji Tobita, 2017, Optics InfoBase Conference Papers, 2017

  DOI
• 二量体化したエーテル型リン脂質膜の異方性および透過性の解析, 土田直之; 高木俊之; 高橋浩; 吉原利忠; 飛田成史; 金森敏幸; 園山正史, 11 Mar. 2015, 日本化学会講演予稿集, 95th, 3, 797
• Optical imaging of hypoxic cells and tissues by using phosphorescent probes, Seiji Tobita; Toshitada Yoshihara; Tsuyoshi Masuda; Taisuke Hatakeyama; Daichi Fujikura; Ryohei Kikuchi; Masahiro Hosaka; Toshiyuki Takeuchi, 17 Aug. 2014, Transactions of Japanese Society for Medical and Biological Engineering, 52, 58, SY-59

  DOI
• Cytotoxicity and cellular distribution of fluorescently-labeled cationic cyclopeptides, Keiichi Yamada; Jin Nozaki; Saori Murayama; Toshitada Yoshihara; Hiroyuki Oku; Seiji Tobita, 2014, 2013, 467, 470
• Gタンパク質共役型受容体、G2Aはヒトアストロサイトーマ細胞株のがん幹細胞性を制御する, 岸本 幸治; 原口 崇; 清水 健志; 山口 藍子; 吉原 利忠; 大島 紀安; 立井 一明; 飛田 成史; 飯田 靖彦; 対島 義人; 和泉 孝志, Sep. 2013, 日本生化学会大会プログラム・講演要旨集, 86回, 1T10a, 05
• Oxidized Free Fatty Acids-Activated GPCR, G2A Regulates Stemness and Malignant Behavior of An Astrocytoma Cell Line., Koji Kishimoto; Takashi Haraguchi; Kenji Shimizu; Aiko Yamaguchi; Toshitada Yoshihara; Noriyasu Ohshima; Kazuaki Tatei; Seiji Tobita; Yoshito Tsushima; Takashi Izumi, Oct. 2012, Society for Neuroscience 2012 Abstract (WEB), 558.04/R19
• G2Aはアストロサイトーマ細胞の幹細胞性と悪性度を制御する(G2A Regulates Stemness and malignant behavior of Astrocytoma Cells), 岸本 幸治; 谷畑 貴俊; 原口 崇; 清水 健志; 山口 藍子; 吉原 利忠; 大島 紀安; 立井 一明; 飛田 成史; 対馬 義人; 和泉 孝志, Aug. 2012, 日本癌学会総会記事, 71回, 288, 288
• 酸化遊離脂肪酸をリガンドとするGタンパク質共役型受容体、G2Aはアストロサイトーマ細胞株のがん幹細胞性を制御し、悪性度を規定する, 岸本幸治; 谷畑貴俊; 原口崇; 清水健志; 山口藍子; 吉原利忠; 大嶋紀安; 立井一明; 飛田成史; 対馬義人; 和泉孝志, Jun. 2012, 平成24年度 日本生化学会関東支部例会 プログラム・講演要旨集, 22, 22
• Iridium complex probes for monitoring of cellular oxygen levels and imaging of hypoxic tissues, Toshitada Yoshihara; Atsushi Kobayashi; Shinpei Oda; Masahiro Hosaka; Toshiyuki Takeuchi; Seiji Tobita, 2012, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 8233

  DOI
• Phosphorescent light-emitting iridium complexes serve as a hypoxia-sensing probe for tumor imaging in living animals, Toshiyuki Takeuchi; Shaojuan Zhang; Kazuya Negishi; Toshitada Yoshihara; Masahiro Hosaka; Seiji Tobita, 2010, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7576

  DOI
• Phosphorescent light-emitting iridium complexes serve as a hypoxia-sensing probe for tumor imaging in living animals, Toshiyuki Takeuchi; Shaojuan Zhang; Kazuya Negishi; Toshitada Yoshihara; Masahiro Hosaka; Seiji Tobita, 2010, REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS II, 7576

  DOI
• In-vivo phosphorescence imaging of cancer using iridium complexes, Toshitada Yoshihara; Yasuyuki Karasawa; Shaojuan Zhang; Masahiro Hosaka; Toshiyuki Takeuchi; Yasuhiko Iida; Keigo Endo; Takashi Imamura; Seiji Tobita, 2009, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7190

  DOI
• Stepwise electron injection in the dye-sensitized nanocrystalline films of ZnO and TiO2 with novel coumarin dye, A Furube; R Katoh; T Yoshihara; K Hara; S Murata; H Arakawa; M Tachiya, 2004, FEMTOCHEMISTRY AND FEMTOBIOLOGY: ULTRAFAST EVENTS IN MOLECULAR SCIENCE, 525, 528

  DOI
• Time-resolved spectroscopy and microscopy on nanocrystalline TiO2 and ZnO films, A Furube; R Katoh; T Yoshihara; Tamaki, V; M Murai; K Hara; S Murata; H Arakawa; M Tachiya, 2004, NANOPHOTONICS: INTEGRATING PHOTOCHEMISTRY, OPTICS AND NANO/BIO MATERIALS STUDIES, 1, 103, 117

Books etc

• 蛍光性脂質滴プローブを用いた細胞および組織内脂質滴のライブイメージング, Contributor, 12 Mar. 2021
• 化学便覧 基礎編 改訂6版, Contributor, Jan. 2021
• 実験医学, Contributor, Jun. 2020
• Quenched-phosphorescence Detection of Molecular Oxygen: Applications in Life Sciences, Contributor, Toshitada Yoshihara; Yosuke Hirakawa; Masaomi Nangaku; Seiji Tobita, Hydrophilic Ir(iii) Complexes for In vitro and In vivo Oxygen Imaging, Royal Society of Chemistry, 2018
• ナノテクノロジーが拓く未来の医療, Contributor, 2017
• 光老化科学の最前線, Contributor, 2015
• DOJIN BIOSCIENCE SERIES 22 がんの分子イメージング, Contributor, 2015
• 光化学の事典, Contributor, 2014
• 炭素とケイ素の元素科学, Contributor, 2014
• 実験医学 増刊, Contributor, 2012

Presentations

• りん光性酸素プローブ分子を用いた 生体内酸素計測・イメージング, 吉原 利忠, 第25回 菅原・大西記念癌治療増感シンポジウム, 01 Feb. 2025
• Confocal microscopic oxygen imaging of xenograft tumor based on lifetime measurements using Ir(III) complexes as intravascular and intracellular probes, Toshitada YOSHIHARA, The 5th Gunma International Symposium 2025 (GIS 2025) on Biomolecular Science and Membrane Application, 23 Jan. 2025
• りん光寿命法に基づく細胞内酸素分圧計測のための近赤外りん光性Ir(III)錯体の光物理特性, 吉原 利忠 ・ 福田 拓弥 ・ 堀越 葵衣 ・ 塩崎 秀一, 2024年光化学討論会, 05 Sep. 2024
• In vivo oxygen imaging of deeper tissues based on phosphorescence lifetime imaging microscopy using near-infrared emitting Ir(III) complexes, Toshitada YOSHIHARA, Kiichi MIZUKAMI, Takako MURAOKA, Shuichi SHIOZAKI, Seiji TOBITA, The 29th IUPAC Symposium on Photochemistry, 18 Jul. 2024
• BODIPY類を骨格とする赤色および深赤色蛍光性分子による細胞および組織内脂質滴の可視化, 吉原 利忠・宇田 梨紗・飯島 鈴七・嶋貫 真由子・塩崎 秀一, 日本化学会第104春季年会(2024), 21 Mar. 2024
• In vivo oxygen imaging of zenograft tumor using Ir(III) complexes as intravascular and intracellular oxygen probes, Toshitada Yoshihara, Takuto Tamura, Shuichi Shiozaki, Seiji Tobita, 第11回武田科学振興財団薬科学シンポジウム, 26 Jan. 2024
• Oxygen imaging of living tissues based on phosphorescence lifetime imaging microscopy, Toshitada Yoshihara, International Society on Oxygen Transport to Tissue (ISOTT) 2023 in Tokyo, 28 Sep. 2023
• りん光寿命イメージング顕微鏡を用いた非アルコール性脂肪肝モデルマウスの肝臓組織内の脂質滴および酸素化状態イメージング, 吉原 利忠・プレブスレン ホラン・塩崎 秀一・飛田 成史, 2023年光化学討論会, 05 Sep. 2023
• In vivo oxygen sensing of pancreatic tissues based on phosphorescence lifetime imaging microscopy, Toshitada Yoshihara, The 31st International Conference on Photochemistry, 25 Jul. 2023
• 脂溶性イリジウム(III)錯体を用いた非アルコール性脂肪肝モデルマウスの肝臓の酸素分圧イメージング, 吉原利忠、プレブスレンホラン、塩崎秀一、飛田成史, 第17回日本分子イメージング学会総会・学術集会, 08 Jun. 2023
• りん寿命顕微分光法による組織内酸素分圧イメージング, 吉原 利忠, 日本薬学会 第143年会（札幌）, 26 Mar. 2023
• ミトコンドリア呼吸依存的な細胞内酸素消費速度の測定法の開発, ミトコンドリア呼吸依存的な細胞内酸素消費速度の測定法の開発, 第21回 日本ミトコンドリア学会年会, 16 Mar. 2023
• Oxygen sensing of pancreatic tissues based on phosphorescence lifetime imaging microscopy using iridium(III) complexes, Toshitada YOSHIHARA, International Conference on Advanced Biomedical Imaging, 09 Jan. 2023
• りん光プローブを用いた生体内酸素計測, 吉原 利忠, in vivoイメージングフォーラム2022, 13 Dec. 2022
• りん光寿命法を用いた生体内酸素計測, 吉原 利忠, 第18回がんとハイポキシア研究会, 26 Nov. 2022
• イリジウム錯体を酸素プローブとして用いたりん光寿命イメージング顕微分光法に基づく膵臓組織の細胞および血中酸素分圧計測, 吉原 利忠、松村 菜生、田村 拓人、塩崎 秀一、飛田 成史, 2022年光化学討論会, 13 Sep. 2022
• イリジウム錯体で標識されたオリゴアルギニンペプチドを用いた血管イメージング, 吉原 利忠、安カ川 真美、鷲見 さくら、塩崎 秀一、飛田 成史, 第44回日本光医学・光生物学会, 25 Jun. 2022
• 蛍光性脂肪滴プローブを用いた細胞および組織内脂肪滴の可視化, 吉原利忠，丸山凌，渋田優妃，塩崎秀一，飛田成史, 第64回日本脂質生化学会, 23 Jun. 2022
• 緑色および赤色りん光性イリジウム錯体のりん光寿命計測に基づく膵臓組織の酸素分圧イメージング, 吉原利忠、松村菜生、田村拓人、塩崎秀一、飛田成史, 第16回日本分子イメージング学会総会・学術集会, 26 May 2022
• オリゴアルギニンペプチドに結合したりん光性Ir(III)錯体とりん光寿命イメージング顕微鏡を用いたin vivo血管イメージング, 吉原利忠, 安カ川真美, 鷲見さくら, 塩崎秀一, 飛田成史, 2021年光化学討論会, 16 Sep. 2021
• 蛍光寿命イメージング法を用いたin vivo脂質滴イメージング, 吉原利忠, 丸山凌, 塩崎秀一, 飛田成史, 第43回日本光医学・光生物学会, 02 Jul. 2021
• クマリン類を配位子に有するイリジウム錯体を用いた生体内酸素レベル計測, 吉原利忠・広瀬 達也・塩崎 秀一・飛田 成史, 第42回日本光医学・光生物学会, 23 Jan. 2021
• Visualization of hypoxia cells in tissues by using phosphorescence lifetime imaging microscopy, Toshitada YOSHIHARA, 第58回日本生物物理学会年会, 17 Sep. 2020
• 蛍光寿命イメージング顕微分光法を用いた細胞および組織内脂質滴のライブイメージング, 吉原 利忠 ・ 丸山 凌 ・ 塩崎 秀一 ・ 飛田 成史, 2020web年光化学討論会, 09 Sep. 2020
• 水溶性イリジウム錯体の開発および 血中酸素イメージング, 吉原 利忠; 綿貫 千優; 水上 輝市; 飛田 成史, 第41回日本光医学・光生物学会, 20 Jul. 2019, Japanese, Domestic conference
• 細胞および血中酸素プローブを用いた 組織内酸素分圧イメージング, 吉原 利忠; 松村 菜生; 湯浅 志保; 塩崎 秀一; 飛田 成史, 第14回日本分子イメージング学会総会・学術集会, 23 May 2019, Japanese, Domestic conference
• 水溶性イリジウム(III)錯体を用いた 腎臓の血中酸素イメージング, 吉原 利忠; 水上 輝市; 平川 陽亮; 南学 正臣; 飛田 成史, 日本化学会第99春季年会, 16 Mar. 2019, Japanese, Domestic conference
• Green-emitting Iridium(III) Complexes with Phenylpyridine Ligands for Oxygen Sensing in Living Cells, Toshitada Yoshihara; Saki Sato; Nao Matsumura; Shuichi Shiozaki; Seiji Tobita, The XXVIIth IUPAC Symposium on Photochemistry, Jul. 2018, International conference
• りん光寿命イメージング法による in vivo酸素分圧計測, 吉原 利忠; 飛田 成史, 第41回日本分子生物学会年会, Nov. 2018
• 共焦点りん光寿命イメージング顕微鏡を用いた 組織内酸素分圧計測, 吉原 利忠; 塩崎 秀一; 平川 陽亮; 南学 正臣; 飛田 成史, 第13回日本分子イメージング学会総会・学術集会, May 2018
• 環状シロキサン中における UVB/UVA広帯域 サンスクリーンの光化学特性, 吉原 利忠; 梁島 歩美; 飛田 成史, 第40回日本光医学・光生物学会, Jul. 2018
• カチオン性イリジウム錯体のりん光寿命測定による細胞内酸素レベル計測, 日本化学会第95春季年会(2015), Mar. 2015
• りん光性イリジウム錯体を用いた細胞・組織内酸素レベル計測, 第54回日本生体医工学会, May 2015
• イリジウム錯体のりん光寿命計測による細胞・組織内の酸素濃度定量, 第10回日本分子イメージング学会学術大会, May 2015
• レシオ型分子酸素計の光物理特性および細胞内酸素センシング, 2015年光化学討論会, Sep. 2015
• Tuning Emission Wavelengths of Metal Complexes Operative in Chemical Biology, The 65th Conference of Japan Society of Coordination Chemistry, Sep. 2015
• In Vivo Oxygen Sensing Using Phosphorescence Lifetime Measurements of Cationic Iridium(III) Complexes, The 3rd Conference of the Japanese Association for Hypoxia Biology, Jul. 2015
• Two Dimensional Oxygen Imaging in Living Cells by Using a Gated ICCD Camera, The Fifth International Symposium on Element Innovation, Nov. 2015
• Ratiometric Optical Probes for Intracellular Oxygen Sensing, 2nd International Symposium of Gunma University Medical Innovation, Dec. 2015
• Ratiometric Optical Sensors for Intracellular Oxygen Sensing, Third International Symposium on the Photofunctional Chemistry of Complex Systems, Dec. 2015
• Ratiometric Optical Sensors Based on Dual Emission of a Blue Fluorescent Coumarin and a Red Phosphorescent Cationic Iridium(III) Complex for Oxygen Sensing in Living cells, The International Chemical Congress of Pacific Basin Societies 2015, Dec. 2015
• Developments of Ratiometric Oxygen Probes for Intracellular Oxygen Sensing, 第11回日本分子イメージング学会学術大会, May 2016
• Intracellular and in vivo Oxygen Sensing by Using Phosphorescence Lifetime Methods, Frontiers 2016 Symposium, Dec. 2016
• Two Dimensional Intracellular and in vivo Oxygen Sensing by Using a Gated ICCD Camera, 3rd International Symposium of Gunma University Medical Innovation and 8th International Conference on Advanced Micro-Device Engineering, Dec. 2016
• Ratimetric Small Molecular Probes Based onDual Emission for Intracellular Oxygen Sensing Dual Emission for Intracellular Oxygen Sensing, OSA Biophotonics Congress: Optics in the Life Sciences, Optical Molecular Probes, Imaging and Drug Delivery, Apr. 2017
• Emission Lifetime Images of Living Cells and Tissues Using a Phosphorescence Iridium(III) Complex and a Confocal Lifetime Imaging Microscopy, 4th International Symposium of Gunma University Medical Innovation, Nov. 2017
• イリジウム錯体のりん光寿命計測に基づく腎臓の酸素レベル計測, 2017年光化学討論会, Sep. 2017
• アミノアセトフェノンとビフェニル誘導体を連結させたUVB/UVA広帯域サンスクリーン, 第39回日本光医学・光生物学会, Jul. 2017
• 細胞内多色酸素濃度イメージングを目指した緑色りん光イリジウム錯体の開発, 第12回日本分子イメージング学会学術大会, May 2017
• Phosphorescence Imaging of Living Cells and Hypoxic Tissues Using Iridium Complxes, The third International Symposium on Element Innovation, 2013
• Phosphorescent Molecular Probes for Imaging Oxygen Levels in Living Cells and Hypoxic Tissues, China/Japan Young Chemists Forum: Molecular Imaging for Chemical Biology, 2014
• Phosphorescence Imaging of Tumors Using Iridium Complexes, 14th International Congress of Histochemistry and Cytochemistry, 2012
• 新規低酸素検出発光プローブの開発, 機能性発光プローブ開発と生体機能イメージング, 2007
• イリジウム錯体のりん光を利用した細胞・組織内酸素濃度定量法の開発, 生物発光化学発光研究会第31回学術講演会, 2014
• EXCITED-STATE PROTON TRANSFER OF N-ALKYL ANILINES: EFFECT OF STRUCTURAL CHANGES OF THE AMINO GROUP, XX IUPAC Symposium on Photochemistry, 2004
• Assignment of Absorption Spectra of Electrons and Holes in Nanocrystalline TiO2 Films Studied by Vis/Near-IR Transient Absorption Spectroscopy, The 6th AIST International Syposium on Photoreaction Control and Photofunctional Materials, 2003
• Absorption Spectra of Electron and Hole in Nanocrystalline TiO2 Films Studied by Transient Absorption Spectroscopy, The International Nanophotonics Symposium, 2003
• Intramolecular Charge Transfer with 1-(Phenyl)pyrroles, XIXth IUPAC Symposium Photochemistry, 2002
• Relaxation Processes of Excited Benzoylacetone Derivatives in Solution, XXII INTERNATIONAL CONFERENCE ON PHOTOCHEMISTRY, 2005
• Electron and Hole Transfer Reactions in Nanocrystalline TiO2 Films Studied by Transient Absorption Spectroscopy, XXIth International Conference on Photochemistry, 2003
• EFFECTS OF SUBSTITUENT ON PHOSPHORESCENCE PROPERTIES OF CYCLOMETALATED IRIDIUM(III) COMPLEXES, XXII IUPAC Symposium on Photochemistry, 2008
• EFFECTS OF SUBSTITUENT ON THE PHOSPHORESCENCE PROPERTIES OF CYCLOMETALATED IRIDIUM(III) COMPLEXES WITH 2-PHENYLPYRIDINES, XXIII IUPAC Symposium on Photochemistry, 2010
• Photophysical Properties of iridium complexes bearing trimethylsilyl-substituted benzothenylpyridinato ligands, The Second International Symposium on Element Innovation, 2012
• Fluorescent properties of cholesterol analogs with dansyl group in model membranes and living cells, XXIVth IUPAC Symposium on Photochemistry, 2012
• Developments of hypoxia-sensing probes with high cellular uptake efficiency, The First International Symposium on Element Innovation, 2011
• Quantitative Analysis of Oxygen in Living Cells and Tissues Using Iridium(III) Complexes with Cationic Substituent, 1st International Symposium of Gunma University Medical Innovation and 6th International Conference on Advanced Micro-Device Engineering, 2014
• Developments of Mitochondria-Specific Oxygen Probes Based on Cationic Iridium(III) Complexes, The Fourth International Symposium on Element Innovation, 2014
• Photophysical Properties of Cationic Iridium Complexes with Mitochondria Specifity and Their Intracellular Behavior, The XXVth IUPAC Symposium on Photochemistry, 2014
• 溶液中における2'-アミノアセトフェノン類の励起状態緩和過程, 日本化学会第85春季年会（2005）, 2005
• アミノベンゾ二トリル類のけい光分子プローブへの応用：HSA中でのけい光特性, 2004年光化学討論会, 2004
• o-アミノアセトフェノン誘導体の励起状態緩和過程：メチル基の面外振動の寄与, 分子構造総合討論会2004, 2004
• EXCITED-STATE PROTON TRANSFER OF N-ALKYL ANILINES: EFFECT OF STRUCTURAL CHANGES OF THE AMINO GROUP, XX IUPAC Symposium on Photochemistry, 2004
• 可視―近赤外過渡吸収分光法による酸化チタンナノ微粒子膜中の電子と正孔の吸収スペクトルの帰属と反応過程の追跡, 固体・表面光化学討論会, 2003
• 可視－近赤外過渡吸収法による酸化チタンナノ微粒子膜中の電子と正孔の吸収スペクトル, 光化学討論会, 2003
• Assignment of Absorption Spectra of Electrons and Holes in Nanocrystalline TiO2 Films Studied by Vis/Near-IR Transient Absorption Spectroscopy, The 6th AIST International Syposium on Photoreaction Control and Photofunctional Materials, 2003
• 過渡吸収分光法による酸化チタンナノ微粒子膜中の電子と正孔の吸収スペクトル, 分子構造総合討論会2003, 2003
• Absorption Spectra of Electron and Hole in Nanocrystalline TiO2 Films Studied by Transient Absorption Spectroscopy, The International Nanophotonics Symposium, 2003
• 過渡吸収分光法による酸化チタンナノ微粒子膜の伝導電子の吸収スペクトル：バイアス依存性, 日本化学会第83回春季年会, 2003
• Intramolecular Charge Transfer with 1-(Phenyl)pyrroles, XIXth IUPAC Symposium Photochemistry, 2002
• Relaxation Processes of Excited Benzoylacetone Derivatives in Solution, XXII INTERNATIONAL CONFERENCE ON PHOTOCHEMISTRY, 2005
• 酸化チタンナノ微粒子膜の可視―近赤外過渡吸収スペクトルのpH依存性, 日本化学会第84回春季年会, 2004
• Electron and Hole Transfer Reactions in Nanocrystalline TiO2 Films Studied by Transient Absorption Spectroscopy, XXIth International Conference on Photochemistry, 2003
• リン脂質二重層膜中における6-ヒドロキシ-2-ナフトエ酸アリルエステルの励起状態プロトン移動反応, 2005年光化学討論会, 2005
• イリジウム錯体の室温りん光をプローブとする脂質二分子膜中の酸素の挙動, 第１回分子科学討論会, 2007
• リン脂質二分子膜中におけるダンシルコレステロールの蛍光特性, 2007光化学討論会, 2007
• ダンシルヒドラゾンを発色団に用いた新規コレステロール誘導体の合成と脂質二重層膜プローブへの応用, 分子構造総合討論会, 2006
• 脂質二重層膜中における6-ヒドロキシ-2-ナフトエ酸アルキルエステル類の励起状態プロトン移動反応の研究, 日本化学会第87春季年会, 2007
• 脂質二分子層膜中における6-ヒドロキシ-2-ナフトエ酸アルキルエステル類の励起状態プロトン解離反応の研究, 日本化学会第86春季年会, 2006
• 3-シアノジメチルアニリンを骨格とする新規環境応答型非天然アミノ酸の合成と光物理, 2006光化学討論会, 2006
• 低酸素環境の可視化を目指したりん光プローブ分子の開発および光物理特性, 第2回関東支部大会, 2008
• 細胞内コレステロールを可視化するための蛍光性コレステロールの開発および光物理特性, 日本化学会第88春季年会, 2008
• Ir錯体のりん光特性に及ぼす置換基効果, 有機EL討論会第６回例会, 2008
• EFFECTS OF SUBSTITUENT ON PHOSPHORESCENCE PROPERTIES OF CYCLOMETALATED IRIDIUM(III) COMPLEXES, XXII IUPAC Symposium on Photochemistry, 2008
• 溶液中のイリジウム錯体の光物理：発光波長に及ぼす置換基効果, 2008光化学討論会, 2008
• 環境応答型蛍光性アミノ酸誘導体を含むペプチドの合成と光化学的性質, 日本化学会第８８春季年会, 2008
• （3-ジメチルアミノ）ベンズアミド基を発色団とする新規蛍光アミノ酸誘導体の合成と性質, 日本化学会第1回関東支部大会, 2007
• 環境応答型蛍光発色団を側鎖に持つ非天然アミノ酸誘導体の合成研究, 日本化学会 第2回関東支部大会（2008）, 2008
• 癌のin vivoりん光イメージングを指向したイリジウム錯体の改良, 第４回日本分子イメージング学会学術集会, 2009
• 環境応答型蛍光性非天然アミノ酸で標識された抗菌性ペプチドの溶液および脂質膜中における光物理特性, 日本化学会第89春季年会, 2009
• Ir錯体のりん光特性に及ぼす置換基効果（２）, 有機EL討論会第7回例会, 2008
• イリジウム錯体の燐光を利用した癌の光検出法の開発, 第31回日本光医学・光生物学会, 2009
• ダンシルを発光団に有する蛍光性コレステロールの開発と光物理特性, 2009光化学討論会, 2009
• 微小領域内酸素濃度計測を目指した蛍光・りん光同時測定による酸素センサー分子の設計と開発, 日本化学会第90春季年会, 2010
• EFFECTS OF SUBSTITUENT ON THE PHOSPHORESCENCE PROPERTIES OF CYCLOMETALATED IRIDIUM(III) COMPLEXES WITH 2-PHENYLPYRIDINES, XXIII IUPAC Symposium on Photochemistry, 2010
• イリジウム錯体を用いた癌の光イメージングおよび生体内挙動, 第32回日本光医学・光生物学会, 2010
• 近赤外りん光を示すイリジウム錯体の開発および細胞・組織イメージング, 第１回低酸素研究会, 2013
• 低酸素組織の光イメージングを目指したジピリナート類を配位子に有する近赤外りん光イリジウム錯体の開発, 第7回日本分子イメージング学会, 2013
• UVB-UVA広帯域吸収を示すサンスクリーン剤の開発, 第4回化粧品開発展 COSME2013 アカデミックフォーラム, 2013
• ジピリナート配位子を有するイリジウム錯体を用いた低酸素環境イメージング, 日本化学会第93春季年会(2013), 2013
• Photophysical Properties of iridium complexes bearing trimethylsilyl-substituted benzothenylpyridinato ligands, The Second International Symposium on Element Innovation, 2012
• カチオン性イリジウム錯体を用いた腫瘍のin vivo光イメージング, 第34回日本光医学・光生物学会, 2012
• 細胞内酸素濃度計測を目指したレシオ型酸素プローブ分子の開発, 第6回分子科学討論会 2012東京, 2012
• 低酸素環境イメージングを指向した近赤外光領域にりん光を示すイリジウム錯体の開発, 2012光化学討論会, 2012
• Fluorescent properties of cholesterol analogs with dansyl group in model membranes and living cells, XXIVth IUPAC Symposium on Photochemistry, 2012
• 可視光領域に吸収・蛍光を示すコレステロールアナログの開発と細胞内発光特性, 2011年光化学討論会, 2011
• Developments of hypoxia-sensing probes with high cellular uptake efficiency, The First International Symposium on Element Innovation, 2011
• 生体内低酸素領域を識別するためのイリジウム錯体の開発および生体内発光特性, 第5回分子科学討論会 2011北海道, 2011
• 高い細胞膜透過性を示すイリジウム錯体の開発およびin vivo光イメージング, 第33回日本光医学・光生物学会, 2011
• がんの光イメージングのためのりん光プローブ分子の開発, 第5回日本分子イメージング学会, 2011
• ミトコンドリア局在性を示すイリジウム錯体を用いた分子酸素センサーの開発, 日本化学会第92春季年会(2012), 2012
• 近赤外光領域にりん光を示すイリジウム錯体を用いた癌組織の光イメージング, 2010年光化学討論会, 2010
• 環境応答型蛍光性コレステロールの脂質膜および細胞内発光特性, 第4回分子科学討論会 2010大阪, 2010
• 細胞・組織内酸素濃度イメージングのためのプローブ分子および計測システムの開発, 第35回日本光医学・光生物学会, 2013
• カチオン性イリジウム錯体をのりん光を用いた生細胞・小動物イメージング, 第17回酸素ダイナミクス研究会, 2013
• 細胞膜透過性を向上させた近赤外りん光イリジウム錯体の開発, 2013年光化学討論会, 2013
• イリジウム錯体を用いた生細胞のりん光寿命イメージングと酸素応答, 第7回分子科学討論会2013京都, 2013
• カチオン性イリジウム錯体を用いたミトコンドリア局在性を示す分子酸素センサーの開発, 日本化学会第94春季年会(2014), 2014
• Quantitative Analysis of Oxygen in Living Cells and Tissues Using Iridium(III) Complexes with Cationic Substituent, 1st International Symposium of Gunma University Medical Innovation and 6th International Conference on Advanced Micro-Device Engineering, 2014
• Developments of Mitochondria-Specific Oxygen Probes Based on Cationic Iridium(III) Complexes, The Fourth International Symposium on Element Innovation, 2014
• Photophysical Properties of Cationic Iridium Complexes with Mitochondria Specifity and Their Intracellular Behavior, The XXVth IUPAC Symposium on Photochemistry, 2014
• ミトコンドリア局在性を示すカチオン性イリジウム錯体の光物理特性および細胞内挙動, 2014年光化学討論会, 2014
• 細胞内移行性を向上させたレシオ型酸素プローブ分子の開発, 第8回分子科学総合討論会2014東広島, 2014
• ミトコンドリア局在性を示すカチオン性イリジウム錯体を用いた生細胞イメージング, 第2回低酸素研究会, 2014
• 分子内エネルギー移動反応と内部変換過程を利用したサンスクリーンの開発, 第36回日本光医学・光生物学会, 2014
• ミトコンドリア局在性を示すカチオン性イリジウム錯体の開発および生細胞イメージング, 第9回日本分子イメージング学会学術大会, 2014

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Awards

• 2008
• 2009
• 2010
• 2012
• 2013
• 2013

Research Projects

• Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Gunma University, Apr. 2020, Mar. 2023, Coinvestigator, 20H03618
• In vivo oxygen mapping based on lifetime imaging microscopy using phosphorescent molecule, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory), Grant-in-Aid for Challenging Research (Exploratory), Gunma University, Jun. 2019, Mar. 2021, Principal investigator, 19K22947
• Oxygen imaging of 3D living cells using multi-color oxygen sensing probes, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Gunma University, Apr. 2017, Mar. 2020, Principal investigator, 17H02078
• Developments of a phosphorescence lifetime imaging microscopy and a probe molecule for oxygenation of retina, YOSHIHARA Toshitada, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Grant-in-Aid for Challenging Exploratory Research, Gunma University, Apr. 2015, Mar. 2017, Principal investigator, We developed a phosphorescent probe and a phosphorescence lifetime imaging microscopy to clarify an oxygen level into a blood vessel and a tissue in retina of small animals. The synthesized phosphorescence probe (DTTPH-PEG24) based on iridium complex showed the near infrared emission. The phosphorescence lifetimes of DTTPH-PEG24 which was administered to the rabbit were dependent on the oxygen partial pressure. These results that our system can visualize an oxygenation of retina of small animals., 15K12502

  対象リソースIRI
• Intracellular oxygen imaging using small ratiometric oxygen probes, YOSHIHARA Toshitada, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A), Grant-in-Aid for Young Scientists (A), Gunma University, Apr. 2014, Mar. 2017, Principal investigator, We designed and synthesized ratiometric molecular probes consisting of a blue fluorescent coumarin and a red phosphorescent cationic iridium complex connected by oligo proline of oligo arginine linkers, respectively, for real time sensing of oxygen levels in living cells. The introduction of oligo arginine linkers improved dramatically the cellular uptake efficiency compared to that of oligo proline linkers. We succeed the detection of oxygen levels in living cells using our small ratiometric oxygen probes., 26702011

  対象リソースIRI
• Organelle-specific intracellular oxygen measurements using iridium complex probes, TOBITA Seiji; YOSHIHARA Toshitada, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Gunma University, Apr. 2011, Mar. 2015, Coinvestigator, We synthesized new phosphorescent iridium complexes BTQ-Mito, BTQSA and BTQDM which are internalized into biological cells without any transfection reagents and show specific localization to mitochondrion, endoplasmic reticulum (ER) and lysosome, respectively. These complexes exhibit oxygen-dependent phosphorescence in living cells. Phosphorescence lifetime measurements of living HeLa cells stained with these probes under a microscope enabled us to examine the subcellular oxygen status. The phosphorescence lifetimes of these probes internalized in HeLa cells were calibrated for the oxygen partial pressure of an incubator according to Stern-Volmer analyses, providing the oxygen quenching rate constant and the average lifetime in the absence of oxygen. Using the values of the oxygen quenching rate constant and the average lifetime, the oxygen level of HeLa cells was evaluated by lifetime measurements., 23310157

  対象リソースIRI
• Development of ratiometric molecular probes for monitoring oxygen levels in living cells, YOSHIHARA TOSHITADA, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, 2011, 2013, Principal investigator, Ratiometric molecular probes for the measurement of oxygen levels in lipid membranes and living cells were designed and synthesized. The synthesized molecules showed an oxygen-insensitive fluorescence with coumarin 343 and an oxygen-sensitive phosphorescence with iridium complexes. The ratio of the phosphorescence intensity for the fluorescence intensity increased with the decrease of oxygen concentration in the solution under the presence of lipid membranes. These probes were added to the cell medium and cells were incubated. The fluorescence and phosphorescence images of living cells were obtained by using microscopic techniques. These results exhibited that the ratiometric molecular probe has a high advantage for the measurements and the images of oxygen levels in living cells., 23500511

  対象リソースIRI
• Phosphorescent Light-emitting lridium Complexes Serve as a Hypoxia-sensing Probe for Tumor Imaging in Living Animals., HOSAKA Masahiro; YOSHIHARA Toshitada; IIDA Yasuhiko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project), Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project), 2009, 2011, Coinvestigator, Iridium complex is a promising organic light-emitting diode material for next generation video displays that emits phosphorescence quenched by oxygen. We focused this oxygen-quenching feature for imaging tumor hypoxia. We used Red light-emitting Ir(btp)_2(acac) (BTP)to image nude mouse-transplanted tumors, and succeed to image tumors. Since BTP is easily modifiable, we synthesized BTP analogues with a longer excitation/emission wavelength. One of them, BTPHSA, depicted clear imaging from tumors transplanted 6-7 mm deep from the skin surface. Another analogue, BTPDM, shines brighter than BTP in tumors, since BTPDM has character to access easily and reach in cells. We suggest that iridium complex materials have a vast potential for imaging hypoxic lesions such as tumor tissues., 21200077

  対象リソースIRI
• Developments of phosphorescence probes for oxygen concentration measurements of ex vivo and in vivo environment, YOSHIHARA Toshitada, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Gunma University, 2009, 2010, Principal investigator, The phosphorescence probes for elucidation of oxygen dynamics in lipid bilayers, cells, and tissues were developed. The synthesized iridium complexes showed high intensity phosphorescence in near-infrared region, and dissolved in water. The photochemical and photophysical properties of these complexes in solutions, lipid membranes and cells were revealed. Based on these data, the optical imaging of tumor in nude mice using these complexes was carried out, and iridium complexes have a vast potential for imaging hypoxic lesions., 21700460

  対象リソースIRI

Academic Contribution

• Academic society etc, 25 Nov. 2022, 26 Nov. 2022

Social Contribution

Social Contribution

• SSH活動（桐生高校）, 14 May 2024, 21 Jan. 2025　(Target:　High school students)
• 生体内酸素を光で探る, 12 Aug. 2023, 12 Aug. 2023　(Target:　General)
• 第18回がんとハイポキシア研究会, 25 Nov. 2022, 26 Nov. 2022　(Target:　College students, Graduate students, Researchers, Company)