Nanotechnology/Materials, Biochemistry

Nanotechnology/Materials, Basic physical chemistry

Life sciences, Biomedical engineering

Life sciences, Biomaterials

Photochemistry

Bioimaging

microspectroscopy

Quantitative dissection of radioresistance in cancer cells: oxygen depletion-induced biological versus chemical mechanisms and baseline mechanisms governed by HIF-1β-NRF2/TXNRD1, Takeuchi S., Kobayashi M., Lee PWT, Takahashi I., Yoshihara T., Harada H., No, No, No, 15 May 2026, Neoplasia, 78, 101317, Scientific journal

Multicolor microscopic in vivo imaging of the formation and disappearance of lipid droplets using BODIPY-based red and deep-red fluorescent probes, M. Shimanuki, R. Uda, S. Shiozaki, T. Yoshihara, No, Yes, Yes, 12 Jun. 2025, ACS Applied Bio Materials, 8, 7, 6193, 6202, Scientific journal

Intracellular oxygen sensing using long-phosphorescence-lifetime cationic Ir(III) complexes with coumarin 6 and 545T ligands, A. Horikoshi, T. Hirose, S. Shiozaki, T. Yoshihara, No, Yes, Yes, 04 Feb. 2026, Analytical Chemistry, 98, 6, 4823, 4833, Scientific journal

Visualization of oxygen profile in reconstructed human epidermis by phosphorescence-lifetime imaging microscopy using Ir(III) complex, M. Ochiai-Noguchi, A. Horikoshi, S. Hirakata, T. Yoshihara, No, Yes, Yes, 15 Oct. 2025, Scientific Reports, 15, 36008, Scientific journal

Confocal microscopic oxygen imaging of xenograft tumors using Ir(III) complexes as in vivo intravascular and intracellular probes, T. Yoshihara, T. Tamura, S. Shiozaki, L.-C. Chou, R. Kakuchi, S. Rokudai, Yes, No, Yes, 08 Aug. 2024, Scientific Reports, 14, 18443, Scientific journal

Yes, Yes, Yes, 01 Mar. 2024, 144, 3, 275, 283, Scientific journal

A silyl porphyrin derivative conjugated with 6-deoxy-6-sulfo-α-d-glucopyranose functions as an efficient photosensitizer for photodynamic therapy, Hiroaki Horiuchi, Kota Nishikawa, Nozomi Ishii, Koki Kano, Shunsuke Shinada, Nene Osawa, Aoi Horikoshi,Toshitada Yoshihara, Fumio Sugawara, Kengo Sakaguchi, Tetsuo Okustu, Shinji Katsura, Ichiro Matsuo, Masahiko Oshige, No, No, No, Feb. 2024, Photodiagnosis and Photodynamic Therapy, 45, 103898, Scientific journal

An Optochemical Oxygen Scavenger Enabling Spatiotemporal Control of Hypoxia, Naoya Ieda, Masato Sawada, Runa Oguchi, Masato Itoh, Seina Hirakata, Daisuke Saitoh, Akito Nakao, Mitsuyasu Kawaguchi, Kazunobu Sawamoto, Toshitada Yoshihara, Yasuo Mori, Hidehiko Nakagawa, No, No, No, 12 Apr. 2023, Angewandte Chemie International Edition, 62, 20, e202217585, Scientific journal

Blue-emitting lipid droplet probes based on coumarin dye for multi-color imaging of living cells and fatty livers of mice, Khulan Purevsuren, Yuhi Shibuta, Shuichi Shiozaki, Mina Tsunoda, Kiichi Mizukami, Seiji Tobita, Toshitada Yoshihara, No, Yes, Yes, Apr. 2023, Journal of Photochemistry and Photobiology A: Chemistry, 438, 114562, Scientific journal

In Vivo Imaging of Lipid Droplets and Oxygen Status in Hepatic Tissues of Nonalcoholic Fatty Liver Model Mice Using a Lipophilic Ir(III) Complex, Khulan Purevsuren, Shuichi Shiozaki, Kiichi Mizukami, Seiji Tobita, Toshitada Yoshihara, No, Yes, Yes, 09 Feb. 2023, Analytical Chemistry, 95, 7, 3729, 3735, Scientific journal

Yes, Yes, Yes, 26 Aug. 2022, 53, 2, 58, 65

Determination of the physiological range of oxygen tension in bone marrow monocytes using two-photon phosphorescence lifetime imaging microscopy, Ayako Narazaki, Reito Shimizu, Toshitada Yoshihara, Junichi Kikuta, Reiko Sakaguchi, Seiji Tobita, Yasuo Mori, Masaru Ishii, Keizo Nishikawa, 10 Mar. 2022, Scientific Reports, 12, 3497, Scientific journal

Intracellular and Intravascular Oxygen Sensing of Pancreatic Tissues Based on Phosphorescence Lifetime Imaging Microscopy Using Lipophilic and Hydrophilic Iridium(III) Complexes, Toshitada Yoshihara, Nao Matsumura, Takuto Tamura, Shuichi Shiozaki, Seiji Tobita, Yes, Yes, 25 Feb. 2022, ACS Sensors, 7, 2, 545, 554, Scientific journal

Near-Infrared Emitting Ir(III) Complexes Bearing a Dipyrromethene Ligand for Oxygen Imaging of Deeper Tissues In Vivo, Kiichi Mizukami, Takako Muraoka, Shuichi Shiozaki, Seiji Tobita, Toshitada Yoshihara, Yes, Yes, 15 Feb. 2022, Analytical Chemistry, 94, 6, 2794, 2802, Scientific journal

Augmented Self‐Association by Electrostatic Forces in Thienopyrrole‐Fused Thiadiazoles that Contain an Ester instead of an Ether Linker, Yukako Naito, Ryo Moriguchi, Chitoshi Kitamura, Taisuke Matsumoto, Toshitada Yoshihara, Tsutomu Ishi-i, Yuka Nagata, Hiroki Takeshita, Kazunari Yoshizawa, Yoshihito Shiota, Kazumasa Suzuki, Shin-ichir, 14 Feb. 2022, Chemistry - An Asian Journal, 17, 4, e2021013, Scientific journal

Cyclometalated Iridium(III) Complex–Cationic Peptide Hybrids Trigger Paraptosis in Cancer Cells via an Intracellular Ca2+ Overload from the Endoplasmic Reticulum and a Decrease in Mitochondrial Membra, Chandrasekar Balachandran, Kenta Yokoi, Kana Naito, Jebiti Haribabu, Yuichi Tamura, Masakazu Umezawa, Koji Tsuchiya, Toshitada Yoshihara, Seiji Tobita, Shin Aoki, 21 Nov. 2021, Molecules, 26, 22, 7028, Scientific journal

Osteoclasts adapt to physioxia perturbation through DNA demethylation, Nishikawa, Keizo, Seno, Shigeto, Yoshihara, Toshitada, Narazaki, Ayako, Sugiura, Yuki, Shimizu, Reito, Kikuta, Junichi, Sakaguchi, Reiko, Suzuki, Norio, Takeda, Norihiko, Semba, Hi, 18 Oct. 2021, EMBO Reports, 22, e53035, Scientific journal

Yes, Yes, 28 Sep. 2021, 61, 5, 298, 302, Scientific journal

S,​C,​C- and O,​C,​C-​Bridged Triarylamines and Their Persistent Radical Cations, Shunpei Kataoka, Shuichi Suzuki, Yoshihito Shiota, Kazunari Yoshizawa, Taisuke Matsumoto, Motoko S. Asano, Toshitada Yoshihara, Chitoshi Kitamura, and Shin-ichiro Kato, 01 Sep. 2021, Journal of Organic Chemistry, 86, 18, 12559, 12568, Scientific journal

Membrane properties of amacrocyclic tetraether bisphosphatidylcholine lipid: Effect of a single membrane-​spanning polymethylene cross-​linkage between two head groups of ditetradecylphosphatidylcholi, Tsuchida, Naoyuki; Takagi, Toshiyuki; Takahashi, Hiroshi; Yoshihara, Toshitada; Tobita, Seiji; Sonoyama, Masashi, 01 May 2021, Biochimica et Biophysica Acta, Biomembranes, 1863, 183569

Analysis of the triplet-​state kinetics of a photosensitizer for photoimmunotherapy by fluorescence correlation spectroscopy, Takakura, Hideo; Goto, Yuto; Kitamura, Akira; Yoshihara, Toshitada; Tobita, Seiji; Kinjo, Masataka; Ogawa, Mikako, 01 Mar. 2021, Journal of Photochemistry and Photobiology, A: Chemistry, 408, 113094

Phosphorescent Ir(III) complexes conjugated with oligoarginine peptides serve as optical probes for in vivo microvascular imaging, Yasukagawa, Mami; Shimada, Aya; Shiozaki, Shuichi; Tobita, Seiji; Yoshihara, Toshitada, Yes, Yes, 26 Feb. 2021, Scientific Reports, 11, 4733

A distinctive distribution of hypoxia-​inducible factor-​1α in cultured renal tubular cells with hypoperfusion simulated by coverslip placement, Honda, Tomoko; Hirakawa, Yosuke; Mizukami, Kiichi; Yoshihara, Toshitada; Tanaka, Tetsuhiro; Tobita, Seiji; Nangaku, Masaomi, 09 Jan. 2021, Physiological Reports, 9, e14689

In vivo O2 imaging in hepatic tissues by phosphorescence lifetime imaging microscopy using Ir(III) complexes as intracellular probes, K. Mizukami; A. Katano; S. Shiozaki; T. Yoshihara; N. Goda; S. Tobita, Yes, Dec. 2020, Sci. Rep., 10, 21053, Scientific journal

Spontaneously blinking fluorophores based on nucleophilic addition/dissociation of intracellular glutathione for live-cell super-resolution imaging., Akihiko Morozumi; Mako Kamiya; Shin-Nosuke Uno; Keitaro Umezawa; Ryosuke Kojima; Toshitada Yoshihara; Seiji Tobita; Yasuteru Urano, 28 Apr. 2020, Journal of the American Chemical Society, 142, 9625, 9633

Visualization of Lipid Droplets in Living Cells and Fatty Livers of Mice Based on the Fluorescence of π-Extended Coumarin Using Fluorescence Lifetime Imaging Microscopy, Yoshihara, Toshitada; Maruyama, Ryo; Shiozaki, Shuichi; Yamamoto, Koji; Kato, Shin-ichiro; Nakamura, Yosuke; Tobita, Seiji, Yes, Yes, 11 Mar. 2020, Analytical Chemistry, 92, 4996, 5003, Scientific journal

Synthesis, Structures, and Properties of Neutral and Radical Cationic S,C,C-Bridged Triphenylamines, Shin-ichiro Kato; Takanori Matsuoka; Shuichi Suzuki; Motoko S. Asano; Toshitada Yoshihara; Seiji Tobita; Taisuke Matsumoto; Chitoshi Kitamura, 17 Jan. 2020, Organic Letters, 22, 734, 738, Scientific journal

Absolute quantum yield measurements of near-infrared emission with correction for solvent absorption, R. Hoshi; K. Suzuki; N. Hasebe; T. Yoshihara; S. Tobita, 2020, Anal. Chem., 92, 607, 611, Scientific journal

Chemical transformation of push-pull fluorenones: push-pull dibenzodicyanofulvenes as well as fluorenone- and dibenzodicyanofulvene-tetracyanobutadiene conjugates, S. Kato; T. Kijima; Y. Shiota; T. Abe; S. Kuwako; H. Miyauchi; N. Yoshikawa; K. Yamamoto; K. Yoshizawa; T. Yoshihara; S. Tobita; Y. Nakamura, 2020, Org. Bio. Chem., 18, 4198, 4209, Scientific journal

Design of spontaneously blinking fluorophore for live-cell super resolution imaging based on quantum-chemical calculations, T. Ryo; M. Kamiya; A. Morozumi; Y. Miyazaki; H. Fujioka; A. Nanjo; R. Kojima; T. Komatsu; T. Ueno; K. Hanaoka; T. Yoshihara; S. Tobita; Y. Urano, 2020, Chem. Commun., 56, 13173, 13176, Scientific journal

Fluorescence Quenching-based Assay for Measuring Golgi endo-α-Mannosidase., Kanae Sano; Taiki Kuribara; Nozomi Ishii; Ayumi Kuroiwa; Toshitada Yoshihara; Seiji Tobita; Kiichiro Totani; Ichiro Matsuo, 03 Jun. 2019, Chemistry, an Asian journal, 14, 11, 1965, 1969

Ratiometric oxygen probes with a cell-penetrating peptide for imaging oxygen levels in living cells, Mami Yasukagawa; Keiich Yamada; Seiji Tobita; ToshitadaYoshihara, Yes, Yes, 2019, Journal of Photochemistry and Photobiology A: Chemistry, 383, 111983

Structure-efficiency relationship of photoinduced electron transfer-triggered nitric oxide releasers, Naoya Ieda; Yumina Oka; Toshitada Yoshihara; Seiji Tobita; Takahiro Sasamori; Mitsuyasu Kawaguchi; Hidehiko Nakagawa, 2019, Scientific Reports, 9, 1430

Intravital phosphorescence lifetime imaging of the renal cortex accurately measures renal hypoxia, Yosuke Hirakawa; Kiichi Mizukami; Toshitada Yoshihara; Ippei Takahashi; Purevsuren Khulan; Tomoko Honda; Imari Mimura; Tetsuhiro Tanaka; Seiji Tobita; Masaomi Nangaku, 01 Jun. 2018, Kidney International, 93, 6, 1483, 1489, Scientific journal

Ir(iii) complex-based oxygen imaging of living cells and ocular fundus with a gated ICCD camera, H. Akiyama; I. Takahashi; Y. Shimoda; R. Mukai; T. Yoshihara; S. Tobita, 2018, Photochemical and Photobiological Sciences, 17, 6, 846, 853, Scientific journal

Extension of pi-system of silylated porphyrin derivative for photodynamic therapy, Hiroaki Horiuchi; Asumi Sakai; Shingo Akiyama; Remi Ikeda; Satoshi Ito; Masaru Furuya; Yuta Gomibuchi; Muneki Ichikawa; Toshitada Yoshihara; Seiji Tobita; Tetsuo Okutsu, Apr. 2017, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 339, 19, 24, Scientific journal

Oxygen imaging of living cells and tissues using luminescent molecular probes, Toshitada Yoshihara; Yosuke Hirakawa; Masahiro Hosaka; Masaomi Nangaku; Seiji Tobita, Mar. 2017, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY C-PHOTOCHEMISTRY REVIEWS, 30, 71, 95

Interrogation of metabolic and oxygen states of tumors with fiber-based luminescence lifetime spectroscopy, Maria Lukina; Anna Orlova; Marina Shirmanova; Daniil Shirokov; Anton Pavlikov; Antje Neubauer; Hauke Studier; Wolfgang Becker; Elena Zagaynova; Toshitada Yoshihara; Seiji Tobita; Vladislav Shcheslavskiy, Feb. 2017, OPTICS LETTERS, 42, 4, 731, 734, Scientific journal

Push-pull fluorenones and benzazulenequinones: regioselective [4+2] and [2+2] cycloadditions of benzopentalenequinone derivative and alkynes bearing an aniline moiety, Shin-ichiro Kato; Tomokazu Kijima; Yoshihito Shiota; Toshitada Yoshihara; Seiji Tobita; Kazunari Yoshizawa; Yosuke Nakamura, Oct. 2016, TETRAHEDRON LETTERS, 57, 41, 4604, 4607, Scientific journal

Biological oxygen sensing via two-photon absorption by an Ir(III) complex using a femtosecond fiber laser, Hiroki Moritomo; Akinari Fujii; Yasutaka Suzuki; Toshitada Yoshihara; Seiji Tobita; Jun Kawamata, Sep. 2016, JAPANESE JOURNAL OF APPLIED PHYSICS, 55, 9, 92401, Scientific journal

Intracellular and in vivo oxygen sensing using phosphorescent iridium(III) complexes, Seiji Tobita; Toshitada Yoshihara, Aug. 2016, CURRENT OPINION IN CHEMICAL BIOLOGY, 33, 39, 45

Phosphorescence quenching of neutral and cationic iridium(III) complexes by molecular oxygen and aromatic electron acceptors, Naoya Hasebe; Yonekazu Deguchi; Saori Murayama; Toshitada Yoshihara; Hiroaki Horiuchi; Tetsuo Okutsu; Seiji Tobita, Jun. 2016, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 324, 134, 144, Scientific journal

The effect of central metal on the photodynamic properties of silylated tetraphenylporphyrin derivative, Hiroaki Horiuchi; Keisuke Terashima; Asumi Sakai; Daisuke Suda; Toshitada Yoshihara; Atsushi Kobayashi; Seiji Tobita; Tetsuo Okutsu, May 2016, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 321, 72, 78, Scientific journal

A series of π-extended thiadiazoles fused with electron-donating heteroaromatic moieties: Synthesis, properties, and polymorphic crystals, Shin-Ichiro Kato; Takayuki Furuya; Masashi Nitani; Naoya Hasebe; Yutaka Ie; Yoshio Aso; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura, 22 Dec. 2015, Chemistry - A European Journal, 21, 7, 3115, 3128, Scientific journal

Quantitating intracellular oxygen tension in vivo by phosphorescence lifetime measurement, Yosuke Hirakawa; Toshitada Yoshihara; Mako Kamiya; Imari Mimura; Daichi Fujikura; Tsuyoshi Masuda; Ryohei Kikuchi; Ippei Takahashi; Yasuteru Urano; Seiji Tobita; Masaomi Nangaku, Dec. 2015, SCIENTIFIC REPORTS, 5, 17838, Scientific journal

Ratiometric molecular probes based on dual emission of a blue fluorescent coumarin and a red phosphorescent cationic iridium(III) complex for intracellular oxygen sensing, Toshitada Yoshihara; Saori Murayama; Seiji Tobita, 09 Jun. 2015, Sensors (Switzerland), 15, 6, 13503, 13521, Scientific journal

Intracellular and in Vivo Oxygen Sensing Using Phosphorescent Ir(III) Complexes with a Modified Acetylacetonato Ligand, Toshitada Yoshihara; Masahiro Hosaka; Motoki Terata; Kazuki Ichikawa; Saori Murayama; Asami Tanaka; Masanobu Mori; Hideyuki Itabashi; Toshiyuki Takeuchi; Seiji Tobita, Mar. 2015, ANALYTICAL CHEMISTRY, 87, 5, 2710, 2717, Scientific journal

Absolute Phosphorescence Quantum Yields of Singlet Molecular Oxygen in Solution Determined Using an Integrating Sphere Instrument, Naoya Hasebe; Kengo Suzuki; Hiroaki Horiuchi; Hiromi Suzuki; Toshitada Yoshihara; Tetsuo Okutsu; Seiji Tobita, Feb. 2015, ANALYTICAL CHEMISTRY, 87, 4, 2360, 2366, Scientific journal

Mitochondria-targeted oxygen probes based on cationic iridium complexes with a 5-amino-1, 10-phenanthroline ligand, Toshitada Yoshihara; Saori Murayarria; Tsuyoshi Masuda; Toshiki Kikuchi; Kohei Yoshida; Masahiro Hosaka; Seiji Tobita, Feb. 2015, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 299, 172, 182, Scientific journal

Amphiphilic benzothiadiazole-triphenylamine-based aggregates that emit red light in water, Tsutomu Ishi-i; Ikumi Kitahara; Shimpei Yamada; Yusuke Sanada; Kazuo Sakurai; Asami Tanaka; Naoya Hasebe; Toshitada Yoshihara; Seiji Tobita, 2015, ORGANIC & BIOMOLECULAR CHEMISTRY, 13, 6, 1818, 1828, Scientific journal

Minimal Thioflavin T Modifications Improve Visual Discrimination of Guanine-Quadruplex Topologies and Alter Compound-Induced Topological Structures, Yuka Kataoka; Hiroto Fujita; Yuuya Kasahara; Toshitada Yoshihara; Seiji Tobita; Masayasu Kuwahara, Dec. 2014, ANALYTICAL CHEMISTRY, 86, 24, 12078, 12084, Scientific journal

A spontaneously blinking fluorophore based on intramolecular spirocyclization for live-cell super-resolution imaging., Shin-Nosuke Uno; Mako Kamiya; Toshitada Yoshihara; Ko Sugawara; Kohki Okabe; Mehmet C Tarhan; Hiroyuki Fujita; Takashi Funatsu; Yasushi Okada; Seiji Tobita; Yasuteru Urano, Aug. 2014, Nature chemistry, 6, 8, 681, 9

Mitochondrial Respiratory Function Induces Endogenous Hypoxia, Sara Prior; Ara Kim; Toshitada Yoshihara; Seiji Tobita; Toshiyuki Takeuchi; Masahiro Higuchi, Feb. 2014, PLOS ONE, 9, 2, e88911, Scientific journal

Systematic Structure-Property Investigations on a Series of Alternating Carbazole-Thiophene Oligomers, Shin-ichiro Kato; Satoru Shimizu; Atsushi Kobayashi; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura, Jan. 2014, JOURNAL OF ORGANIC CHEMISTRY, 79, 2, 618, 629, Scientific journal

Tetraalkoxyphenanthrene-fused dehydroannulenes: Synthesis, self-assembly, and electronic, optical, and electrochemical properties, Shin-Ichiro Kato; Nobutaka Takahashi; Hidekazu Tanaka; Atsushi Kobayashi; Toshitada Yoshihara; Seiji Tobita; Takeshi Yamanobe; Hiroki Uehara; Yosuke Nakamura, 02 Sep. 2013, Chemistry - A European Journal, 19, 36, 12138, 12151, Scientific journal

Fluorescent Peptides Labeled with Environment-Sensitive 7-Aminocoumarins and Their Interactions with Lipid Bilayer Membranes and Living Cells, Tokiko Murase; Toshitada Yoshihara; Keiichi Yamada; Seiji Tobita, Apr. 2013, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 86, 4, 510, 519, Scientific journal

Bicarbazoles: Systematic Structure-Property Investigations on a Series of Conjugated Carbazole Dimers, Shin-ichiro Kato; Hiroto Noguchi; Atsushi Kobayashi; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura, Oct. 2012, JOURNAL OF ORGANIC CHEMISTRY, 77, 20, 9120, 9133, Scientific journal

pi-Extended Thiadiazoles Fused with Thienopyrrole or Indole Moieties: Synthesis, Structures, and Properties, Shin-ichiro Kato; Takayuki Furuya; Atsushi Kobayashi; Masashi Nitani; Yutaka Ie; Yoshio Aso; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura, Sep. 2012, JOURNAL OF ORGANIC CHEMISTRY, 77, 17, 7595, 7606, Scientific journal

Environment-Sensitive Fluorophores with Benzothiadiazole and Benzoselenadiazole Structures as Candidate Components of a Fluorescent Polymeric Thermometer, Seiichi Uchiyama; Kohki Kimura; Chie Gota; Kohki Okabe; Kyoko Kawamoto; Noriko Inada; Toshitada Yoshihara; Seiji Tobita, Jul. 2012, CHEMISTRY-A EUROPEAN JOURNAL, 18, 31, 9552, 9563

Mitochondria-specific Oxygen Probe Based on Iridium Complexes Bearing Triphenylphosphonium Cation, Tokiko Murase; Toshitada Yoshihara; Seiji Tobita, Mar. 2012, CHEMISTRY LETTERS, 41, 3, 262, 263, Scientific journal

Remarkable fluorescence enhancement of benzo[g]chromen-2-ones induced by hydrogen-bonding interactions with protic solvents, Atsushi Kobayashi; Kazuyuki Takehira; Toshitada Yoshihara; Seiichi Uchiyama; Seiji Tobita, 2012, PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 11, 8, 1368, 1376, Scientific journal

Ratiometric Molecular Sensor for Monitoring Oxygen Levels in Living Cells, Toshitada Yoshihara; Yuji Yamaguchi; Masahiro Hosaka; Toshiyuki Takeuchi; Seiji Tobita, 2012, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 51, 17, 4148, 4151, Scientific journal

Control of Photophysical Properties of 1,8-Naphthalimides by Electron-Withdrawing Substituents Introduced into N-Alkyl Side Chains, Kazuhiko Matsubayashi; Chisato Kajimura; Yasuo Kubo; Toshitada Yoshihara; Seiji Tobita, May 2011, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 84, 5, 520, 525, Scientific journal

Kinetic Analysis for the Effect of Intramolecular Hydrogen Bonding on Photophysical Properties of N-Hydroxyalkyl-1,8-naphthalimides, Kazuhiko Matsubayashi; Chisato Kajimura; Hideo Shiratori; Yasuo Kubo; Toshitada Yoshihara; Seiji Tobita, Sep. 2010, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 83, 9, 1067, 1073, Scientific journal

Phosphorescent Light-Emitting Iridium Complexes Serve as a Hypoxia-Sensing Probe for Tumor Imaging in Living Animals, Shaojuan Zhang; Masahiro Hosaka; Toshitada Yoshihara; Kazuya Negishi; Yasuhiko Iida; Seiji Tobita; Toshiyuki Takeuchi, Jun. 2010, CANCER RESEARCH, 70, 11, 4490, 4498, Scientific journal

Absolute Measurements of Photoluminescence Quantum Yields of 1-Halonaphthalenes in 77K Rigid Solution Using an Integrating Sphere Instrument, Atsushi Kobayashi; Kengo Suzuki; Toshitada Yoshihara; Seiji Tobita, Mar. 2010, CHEMISTRY LETTERS, 39, 3, 282, 283, Scientific journal

Synthesis and Photophysical Properties of N-Arylcarbazolophanes, Hideyuki Shimizu; Yasuhiko Kakinoya; Kazuyuki Takehira; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura; Jun Nishimura, Jul. 2009, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 82, 7, 860, 869, Scientific journal

SUBSTITUENT EFFECTS ON PHOTOPHYSICAL PROPERTIES OF N-THIENYLCARBAZOLES, Hideyuki Shimizu; Atsushi Kobayashi; Shingo Itoi; Toshitada Yoshihara; Seiji Tobita; Yosuke Nakamura; Jun Nishimura, May 2009, HETEROCYCLES, 78, 5, 1265, 1269, Scientific journal

The Rate of Excited-state Proton Transfer to Solvent from Trifluoromethylphenols in Water, Shigeo Kaneko; Toshitada Yoshihara; Seiji Tobita, Apr. 2009, CHEMISTRY LETTERS, 38, 4, 312, 313, Scientific journal

Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector, Kengo Suzuki; Atsushi Kobayashi; Shigeo Kaneko; Kazuyuki Takehira; Toshitada Yoshihara; Hitoshi Ishida; Yoshimi Shiina; Shigero Oishic; Seiji Tobita, 2009, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 11, 42, 9850, 9860, Scientific journal

Measurement of photoluminescence efficiency of Ir(III) phenylpyridine derivatives in solution and solid-state films, Ayataka Endo; Kengo Suzuki; Toshitada Yoshihara; Seiji Tobita; Masayuki Yahiro; Chihaya Adachi, Jul. 2008, CHEMICAL PHYSICS LETTERS, 460, 1-3, 155, 157, Scientific journal

Temperature-dependent fluorescence lifetime of a fluorescent polymeric thermometer, Poly(N-isopropylacrylamide), labeled by polarity and hydrogen bonding sensitive 4-sulfamoyl-7-aminobenzofurazan, Chie Gota; Seiichi Uchiyama; Toshitada Yoshihara; Seiji Tobita; Tomohiko Ohwada, Mar. 2008, JOURNAL OF PHYSICAL CHEMISTRY B, 112, 10, 2829, 2836, Scientific journal

Photoinduced intramolecular electron transfer of carbazole trimer-[60]fullerene studied by laser flash photolysis techniques, Takashi Konno; Mohamed E. El-Khouly; Yosuke Nakamura; Keisuke Kinoshita; Yasuyuki Araki; Osamu Ito; Toshitada Yoshihara; Seiji Tobita; Jun Nishimura, Jan. 2008, JOURNAL OF PHYSICAL CHEMISTRY C, 112, 4, 1244, 1249, Scientific journal

Extremely low-threshold amplified spontaneous emission of 9,9 '-spirobifluorene derivatives and electroluminescence from field-effect transistor structure, Hajime Nakanotani; Seiji Akiyama; Dai Ohnishi; Masato Moriwake; Masayuki Yahiro; Toshitada Yoshihara; Seiji Tobita; Chihaya Adachi, Sep. 2007, ADVANCED FUNCTIONAL MATERIALS, 17, 14, 2328, 2335, Scientific journal

Effect of pH on absorption spectra of photogenerated holes in nanocrystalline TiO2 films, Toshitada Yoshihara; Yoshiaki Tamaki; Akihiro Furube; Miki Murai; Kohjiro Hara; Ryuzi Katoh, Apr. 2007, CHEMICAL PHYSICS LETTERS, 438, 4-6, 268, 273, Scientific journal

Synthesis and photophysical properties of [60]fullerene adducts carrying oligocarbazole dendrons, Yosuke Nakamura; Takashi Konno; Satoru Watanabe; Masato Suzuki; Toshitada Yoshihara; Seiji Tobita; Jun Nishimura, Feb. 2007, CHEMISTRY LETTERS, 36, 2, 264, 265, Scientific journal

Environment-sensitive fluorophore emitting in protic environments, Seiichi Uchiyama; Kazuyuki Takehira; Toshitada Yoshihara; Seiji Tobita; Tomohiko Ohwada, Dec. 2006, ORGANIC LETTERS, 8, 25, 5869, 5872, Scientific journal

Intramolecular charge transfer and dielectric solvent relaxation in n-propyl cyanide N-phenylpyrrole and 4-Dimethylamino-4 '-cyanostilbene, Sergey I. Druzhinin; Victor A. Galievsky; Toshitada Yoshihara; Klaas A. Zachariasse, Nov. 2006, JOURNAL OF PHYSICAL CHEMISTRY A, 110, 47, 12760, 12768, Scientific journal

Photophysical properties of 3-[2-cyano-4-(dimethylamino)phenyl]alanine: A highly fluorescent and environment-sensitive amino acid with small molecular size, Juro Oshima; Hitomi Tsujimoto; Toshitada Yoshihara; Keiichi Yamada; Ryoichi Katakai; Seiji Tobita, Jun. 2006, CHEMISTRY LETTERS, 35, 6, 620, 621, Scientific journal

Water-induced fluorescence quenching of mono- and dicyanoanilines, J Oshima; T Yoshihara; S Tobita, Jun. 2006, CHEMICAL PHYSICS LETTERS, 423, 4-6, 306, 311, Scientific journal

Extreme fluorescence sensitivity of some aniline derivatives to aqueous and nonaqueous environments: Mechanistic study and its implication as a fluorescent probe, J Oshima; S Shiobara; H Naoumi; S Kaneko; T Yoshihara; AK Mishra; S Tobita, Apr. 2006, JOURNAL OF PHYSICAL CHEMISTRY A, 110, 14, 4629, 4637, Scientific journal

An efficient energy transfer found in triphenylene/hexaazatriphenylene system with electronic complimentarity and structural similarity, T Ishi-i; H Tashiro; R Kuwahara; S Mataka; T Yoshihara; S Tobita, Feb. 2006, CHEMISTRY LETTERS, 35, 2, 158, 159, Scientific journal

Synthesis and complexing properties of [2.n](2,6)pyridinocrownophanes, S Inokuma; H Ide; T Yonekura; T Funaki; S Kondo; S Shiobara; T Yoshihara; S Tobita; J Nishimura, Mar. 2005, JOURNAL OF ORGANIC CHEMISTRY, 70, 5, 1698, 1703, Scientific journal

Kinetics of intramolecular charge transfer with N-phenylpyrrole in alkyl cyanides, T Yoshihara; SI Druzhinin; A Demeter; N Kocher; D Stalke; KA Zachariasse, Mar. 2005, JOURNAL OF PHYSICAL CHEMISTRY A, 109, 8, 1497, 1509, Scientific journal

Novel conjugated organic dyes for efficient dye-sensitized solar cells, K Hara; T Sato; R Katoh; A Furube; T Yoshihara; M Murai; M Kurashige; S Ito; A Shinpo; S Suga; H Arakawa, Feb. 2005, ADVANCED FUNCTIONAL MATERIALS, 15, 2, 246, 252, Scientific journal

The exo-outstretching effect governing the exclusive stereoselectivity of the intramolecular [2+2] photocycloaddition of vinylarenes, J Nishimura; T Funaki; N Saito; S Inokuma; Y Nakamura; S Tajima; T Yoshihara; S Tobita, 2005, HELVETICA CHIMICA ACTA, 88, 6, 1226, 1239, Scientific journal

Intramolecular and intermolecular hydrogen-bonding effects on photophysical properties of 2 '-aminoacetophenone and its derivatives in solution, H Shimada; A Nakamura; T Yoshihara; S Tobita, 2005, PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 4, 4, 367, 375, Scientific journal

Ultrafast direct and indirect electron-injection processes in a photoexcited dye-sensitized nanocrystalline zinc oxide film: The importance of exciplex intermediates at the surface, A Furube; R Katoh; T Yoshihara; K Hara; S Murata; H Arakawa; M Tachiya, Aug. 2004, JOURNAL OF PHYSICAL CHEMISTRY B, 108, 33, 12583, 12592, Scientific journal

Trace analysis by transient absorption spectroscopy: estimation of the solubility of C-60 in polar solvents, T Yoshihara; M Murai; Y Tamaki; A Furube; R Katoh, Aug. 2004, CHEMICAL PHYSICS LETTERS, 394, 1-3, 161, 164, Scientific journal

Microscopic imaging of the efficiency of electron injection from excited sensitizer dye into nanocrystalline ZnO film, R Katoh; A Furube; Y Tamaki; T Yoshihara; M Murai; K Hara; S Murata; H Arakawa; M Tachiya, Aug. 2004, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 166, 1-3, 69, 74, Scientific journal

Fast intramolecular charge transfer with a planar rigidized electron donor/acceptor molecule, T Yoshihara; SI Druzhinin; KA Zachariasse, Jul. 2004, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 126, 27, 8535, 8539, Scientific journal

Efficiencies of electron injection from excited N3 dye into nanocrystalline semiconductor (ZrO2, TiO2, ZnO, Nb2O5, SnO2, In2O3) films, R Katoh; A Furube; T Yoshihara; K Hara; G Fujihashi; S Takano; S Murata; H Arakawa; M Tachiya, Apr. 2004, JOURNAL OF PHYSICAL CHEMISTRY B, 108, 15, 4818, 4822, Scientific journal

A new-type photoreaction of a carbonyl compound - Part 1. Photoinduced ω-bond dissociation in p-mercaptomethylbenzophenone studied by time-resolved EPR technique, steady-state and flaser flash photolyses in solution, Minoru Yamaji; Toshitada Yoshihara; Takashi Tachikawa; Shozo Tero-Kubota; Seiji Tobita; Haruo Shizuka; Bronislaw Marciniak, 15 Mar. 2004, Journal of Photochemistry and Photobiology A: Chemistry, 162, 2-3, 513, 520, Scientific journal

Identification of reactive species in photoexcited nanocrystalline TiO2 films by wide-wavelength-range (400-2500 nm) transient absorption spectroscopy, T Yoshihara; R Katoh; A Furube; Y Tamaki; M Murai; K Hara; S Murata; H Arakawa; M Tachiya, Mar. 2004, JOURNAL OF PHYSICAL CHEMISTRY B, 108, 12, 3817, 3823, Scientific journal

Quantitative estimation of the efficiency of electron injection from excited sensitizer dye into nanocrystalline ZnO film, T Yoshihara; R Katoh; A Furube; M Murai; Y Tamaki; K Hara; S Murata; H Arakawa; M Tachiya, Feb. 2004, JOURNAL OF PHYSICAL CHEMISTRY B, 108, 8, 2643, 2647, Scientific journal

Thermally activated internal conversion with 4-(dimethylamino)benzonitrile, 4-(methylamino)benzonitrile, and 4-aminobenzonitrile in alkane solvents. No correlation with intramolecular charge transfer, SI Druzhinin; A Demeter; VA Galievsky; T Yoshihara; KA Zachariasse, Oct. 2003, JOURNAL OF PHYSICAL CHEMISTRY A, 107, 40, 8075, 8085, Scientific journal

Singlet excited state dipole moments of dual fluorescent N-phenylpyrroles and 4-(dimethylamino)benzonitrile from solvatochromic and thermochromic spectral shifts, T Yoshihara; VA Galievsky; SI Druzhinin; S Saha; KA Zachariasse, Mar. 2003, PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2, 3, 342, 353, Scientific journal

Picosecond and nanosecond fluorescence decays of 4-(dimethylamino)phenylacetylene in comparison with those of 4-(dimethylamino)benzonitrile. No evidence for intramolecular charge transfer and a nonfluorescing intramolecular charge-transfer state, KA Zachariasse; T Yoshihara; SI Druzhinin, Jul. 2002, JOURNAL OF PHYSICAL CHEMISTRY A, 106, 26, 6325, 6333, Scientific journal

Photochemistry of 1,4-anthraquinone studied by steady-state and laser-flash photolysis, T Yoshihara; M Yamaji; T Itoh; J Nishimura; H Shizuka; S Tobita, Apr. 2001, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 140, 1, 7, 13, Scientific journal

Internal conversion of o-aminoacetophenone in solution, T Yoshihara; H Shimada; H Shizuka; S Tobita, 2001, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 3, 22, 4972, 4978, Scientific journal

Radiationless processes of N,N-dimethylaniline derivatives in solution. Time-resolved optoacoustic studies., S Tobita; R Kamiyama; K Takehira; T Yoshihara; S Yotoriyama; H Shizuka, 2001, ANALYTICAL SCIENCES, 17, S50, S52, Scientific journal

Hydrogen atom transfer and electron transfer reactions in the triplet π,π* state of 1,4-anthraquinone studied by CIDEP techniques and laser flash photolysis, Toshitada Yoshihara; Minoru Yamaji; Takao Itoh; Haruo Shizuka; Takuji Shimokage; Shozo Tero-Kubota, 01 Mar. 2000, Physical Chemistry Chemical Physics, 2, 5, 993, 1000, Scientific journal

Laser photolysis studies on hydrogen atom abstraction by triplet benzophenone from hydroxyanilinium ions, Toshitada Yoshihara; Minoru Yamaji; Haruo Shizuka, 25 Oct. 1996, Chemical Physics Letters, 261, 4-5, 431, 436, Scientific journal

No, Yes, Yes, 20 Jun. 2022, 54, 7, 16（368）, 19（371）

Apr. 2018

In-vivo phosphorescence imaging of cancer using iridium complexes, Toshitada Yoshihara; Yasuyuki Karasawa; Shaojuan Zhang; Masahiro Hosaka; Toshiyuki Takeuchi; Yasuhiko Iida; Keigo Endo; Takashi Imamura; Seiji Tobita, 2009, REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS, 7190, 71900X-1-71900X-8

Iridium complex probes for monitoring of cellular oxygen levels and imaging of hypoxic tissues, Toshitada Yoshihara; Atsushi Kobayashi; Shinpei Oda; Masahiro Hosaka; Toshiyuki Takeuchi; Seiji Tobita, 2012, REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS IV, 8233, 82330A-1-82330A-8

Cyclic b-Sheet Antimicrobial Peptides with Enviroment-sensitive Fluorophores: Photophyscial Properties and Molecular Orientation in Lipid Bilayers,, K. Yamada; T. Yoshihara; T. Katsu; H. Oku; S. Tobita, 2009, Peptide Science, 45, 281

Ex vivo and in vivo behavior of red and near infrared-emitting iridium(III) complexes, T. Yoshihara; Y. Ohori; Y. Karasawa; M. Hosaka; T. Takeuchi; S. Tobita, 2010, Photomedicine and Photobiology, 32, 39, 40

Synthesis and biological evaluation of fluorescent analogs of polymyxin B, H. Suda; J. Nozaki; T. Yoshihara; K. Komagoe; H. Oku; S. Tobita; T. Katsu; K. Yamada, 2011, Peptide Science, 48, 97, 100

2007, 7, 9, 363

Photophysical study of iridium complexes by absolute photoluminescence quantum yield measurements using an integrating sphere, Kengo Suzuki; Ayataka Endo; Toshitada Yoshihara; Seiji Tobita; Masayuki Yahiro; Daisuke Yokoyama; Chihaya Adachi, 2009, Proceedings of SPIE - The International Society for Optical Engineering, 7415, 741504/1-741504/8

2009, 31, 13

2010, 3, 26

2010, 55, 584

2009, 45, 281

2009, 35, 538

2010, 46, 127, 130

2010, 47, 268

2010, 32, 39, 40

2011, 33, 15, 16

2011, 48, 97, 100

2012, 30, 1100, 1106

2017, 53, 3, 220, 224

2013, 45, 9, 431, 434

2013, 45, 12, 592, 595

2013, 44, 2, 86, 89

Ratiometric small molecular probes based on dual emission for intracellular oxygen sensing, Toshitada Yoshihara; Seiji Tobita, 2017, Optics InfoBase Conference Papers, 2017

二量体化したエーテル型リン脂質膜の異方性および透過性の解析, 土田直之; 高木俊之; 高橋浩; 吉原利忠; 飛田成史; 金森敏幸; 園山正史, 11 Mar. 2015, 日本化学会講演予稿集, 95th, 3, 797

Optical imaging of hypoxic cells and tissues by using phosphorescent probes, Seiji Tobita; Toshitada Yoshihara; Tsuyoshi Masuda; Taisuke Hatakeyama; Daichi Fujikura; Ryohei Kikuchi; Masahiro Hosaka; Toshiyuki Takeuchi, 17 Aug. 2014, Transactions of Japanese Society for Medical and Biological Engineering, 52, 58, SY-59

2014, 2013, 467, 470

Gタンパク質共役型受容体、G2Aはヒトアストロサイトーマ細胞株のがん幹細胞性を制御する, 岸本 幸治; 原口 崇; 清水 健志; 山口 藍子; 吉原 利忠; 大島 紀安; 立井 一明; 飛田 成史; 飯田 靖彦; 対島 義人; 和泉 孝志, Sep. 2013, 日本生化学会大会プログラム・講演要旨集, 86回, 1T10a, 05

Oxidized Free Fatty Acids-Activated GPCR, G2A Regulates Stemness and Malignant Behavior of An Astrocytoma Cell Line., Koji Kishimoto; Takashi Haraguchi; Kenji Shimizu; Aiko Yamaguchi; Toshitada Yoshihara; Noriyasu Ohshima; Kazuaki Tatei; Seiji Tobita; Yoshito Tsushima; Takashi Izumi, Oct. 2012, Society for Neuroscience 2012 Abstract (WEB), 558.04/R19

G2Aはアストロサイトーマ細胞の幹細胞性と悪性度を制御する(G2A Regulates Stemness and malignant behavior of Astrocytoma Cells), 岸本 幸治; 谷畑 貴俊; 原口 崇; 清水 健志; 山口 藍子; 吉原 利忠; 大島 紀安; 立井 一明; 飛田 成史; 対馬 義人; 和泉 孝志, Aug. 2012, 日本癌学会総会記事, 71回, 288, 288

酸化遊離脂肪酸をリガンドとするGタンパク質共役型受容体、G2Aはアストロサイトーマ細胞株のがん幹細胞性を制御し、悪性度を規定する, 岸本幸治; 谷畑貴俊; 原口崇; 清水健志; 山口藍子; 吉原利忠; 大嶋紀安; 立井一明; 飛田成史; 対馬義人; 和泉孝志, Jun. 2012, 平成24年度 日本生化学会関東支部例会 プログラム・講演要旨集, 22, 22

Iridium complex probes for monitoring of cellular oxygen levels and imaging of hypoxic tissues, Toshitada Yoshihara; Atsushi Kobayashi; Shinpei Oda; Masahiro Hosaka; Toshiyuki Takeuchi; Seiji Tobita, 2012, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 8233

Phosphorescent light-emitting iridium complexes serve as a hypoxia-sensing probe for tumor imaging in living animals, Toshiyuki Takeuchi; Shaojuan Zhang; Kazuya Negishi; Toshitada Yoshihara; Masahiro Hosaka; Seiji Tobita, 2010, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7576

Phosphorescent light-emitting iridium complexes serve as a hypoxia-sensing probe for tumor imaging in living animals, Toshiyuki Takeuchi; Shaojuan Zhang; Kazuya Negishi; Toshitada Yoshihara; Masahiro Hosaka; Seiji Tobita, 2010, REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS II, 7576

In-vivo phosphorescence imaging of cancer using iridium complexes, Toshitada Yoshihara; Yasuyuki Karasawa; Shaojuan Zhang; Masahiro Hosaka; Toshiyuki Takeuchi; Yasuhiko Iida; Keigo Endo; Takashi Imamura; Seiji Tobita, 2009, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7190

Stepwise electron injection in the dye-sensitized nanocrystalline films of ZnO and TiO2 with novel coumarin dye, A Furube; R Katoh; T Yoshihara; K Hara; S Murata; H Arakawa; M Tachiya, 2004, FEMTOCHEMISTRY AND FEMTOBIOLOGY: ULTRAFAST EVENTS IN MOLECULAR SCIENCE, 525, 528

Time-resolved spectroscopy and microscopy on nanocrystalline TiO2 and ZnO films, A Furube; R Katoh; T Yoshihara; Tamaki, V; M Murai; K Hara; S Murata; H Arakawa; M Tachiya, 2004, NANOPHOTONICS: INTEGRATING PHOTOCHEMISTRY, OPTICS AND NANO/BIO MATERIALS STUDIES, 1, 103, 117

Contributor, 12 Mar. 2021

Contributor, Jan. 2021

Contributor, Jun. 2020

Quenched-phosphorescence Detection of Molecular Oxygen: Applications in Life Sciences, Contributor, Toshitada Yoshihara; Yosuke Hirakawa; Masaomi Nangaku; Seiji Tobita, Hydrophilic Ir(iii) Complexes for In vitro and In vivo Oxygen Imaging, Royal Society of Chemistry, 2018

Contributor, 2017

Contributor, 2015

Contributor, 2015

Contributor, 2014

Contributor, 2014

Contributor, 2012

29 May 2025

01 Feb. 2025

Confocal microscopic oxygen imaging of xenograft tumor based on lifetime measurements using Ir(III) complexes as intravascular and intracellular probes, Toshitada YOSHIHARA, The 5th Gunma International Symposium 2025 (GIS 2025) on Biomolecular Science and Membrane Application, 23 Jan. 2025

05 Sep. 2024

In vivo oxygen imaging of deeper tissues based on phosphorescence lifetime imaging microscopy using near-infrared emitting Ir(III) complexes, Toshitada YOSHIHARA, Kiichi MIZUKAMI, Takako MURAOKA, Shuichi SHIOZAKI, Seiji TOBITA, The 29th IUPAC Symposium on Photochemistry, 18 Jul. 2024

21 Mar. 2024

In vivo oxygen imaging of zenograft tumor using Ir(III) complexes as intravascular and intracellular oxygen probes, Toshitada Yoshihara, Takuto Tamura, Shuichi Shiozaki, Seiji Tobita, 26 Jan. 2024

Oxygen imaging of living tissues based on phosphorescence lifetime imaging microscopy, Toshitada Yoshihara, International Society on Oxygen Transport to Tissue (ISOTT) 2023 in Tokyo, 28 Sep. 2023

05 Sep. 2023

In vivo oxygen sensing of pancreatic tissues based on phosphorescence lifetime imaging microscopy, Toshitada Yoshihara, The 31st International Conference on Photochemistry, 25 Jul. 2023

08 Jun. 2023

26 Mar. 2023

16 Mar. 2023

Oxygen sensing of pancreatic tissues based on phosphorescence lifetime imaging microscopy using iridium(III) complexes, Toshitada YOSHIHARA, International Conference on Advanced Biomedical Imaging, 09 Jan. 2023

13 Dec. 2022

26 Nov. 2022

13 Sep. 2022

25 Jun. 2022

23 Jun. 2022

26 May 2022

16 Sep. 2021

02 Jul. 2021

23 Jan. 2021

Visualization of hypoxia cells in tissues by using phosphorescence lifetime imaging microscopy, Toshitada YOSHIHARA, 17 Sep. 2020

09 Sep. 2020

20 Jul. 2019, Japanese, Domestic conference

23 May 2019, Japanese, Domestic conference

16 Mar. 2019, Japanese, Domestic conference

Green-emitting Iridium(III) Complexes with Phenylpyridine Ligands for Oxygen Sensing in Living Cells, Toshitada Yoshihara; Saki Sato; Nao Matsumura; Shuichi Shiozaki; Seiji Tobita, The XXVIIth IUPAC Symposium on Photochemistry, Jul. 2018, International conference

Nov. 2018

May 2018

Jul. 2018

Mar. 2015

May 2015

May 2015

Sep. 2015

Tuning Emission Wavelengths of Metal Complexes Operative in Chemical Biology, The 65th Conference of Japan Society of Coordination Chemistry, Sep. 2015

In Vivo Oxygen Sensing Using Phosphorescence Lifetime Measurements of Cationic Iridium(III) Complexes, The 3rd Conference of the Japanese Association for Hypoxia Biology, Jul. 2015

Two Dimensional Oxygen Imaging in Living Cells by Using a Gated ICCD Camera, The Fifth International Symposium on Element Innovation, Nov. 2015

Ratiometric Optical Probes for Intracellular Oxygen Sensing, 2nd International Symposium of Gunma University Medical Innovation, Dec. 2015

Ratiometric Optical Sensors for Intracellular Oxygen Sensing, Third International Symposium on the Photofunctional Chemistry of Complex Systems, Dec. 2015

Ratiometric Optical Sensors Based on Dual Emission of a Blue Fluorescent Coumarin and a Red Phosphorescent Cationic Iridium(III) Complex for Oxygen Sensing in Living cells, The International Chemical Congress of Pacific Basin Societies 2015, Dec. 2015

Developments of Ratiometric Oxygen Probes for Intracellular Oxygen Sensing, 第11回日本分子イメージング学会学術大会, May 2016

Intracellular and in vivo Oxygen Sensing by Using Phosphorescence Lifetime Methods, Frontiers 2016 Symposium, Dec. 2016

Two Dimensional Intracellular and in vivo Oxygen Sensing by Using a Gated ICCD Camera, 3rd International Symposium of Gunma University Medical Innovation and 8th International Conference on Advanced Micro-Device Engineering, Dec. 2016

Ratimetric Small Molecular Probes Based onDual Emission for Intracellular Oxygen Sensing Dual Emission for Intracellular Oxygen Sensing, OSA Biophotonics Congress: Optics in the Life Sciences, Optical Molecular Probes, Imaging and Drug Delivery, Apr. 2017

Emission Lifetime Images of Living Cells and Tissues Using a Phosphorescence Iridium(III) Complex and a Confocal Lifetime Imaging Microscopy, 4th International Symposium of Gunma University Medical Innovation, Nov. 2017

Sep. 2017

Jul. 2017

May 2017

Phosphorescence Imaging of Living Cells and Hypoxic Tissues Using Iridium Complxes, The third International Symposium on Element Innovation, 2013

Phosphorescent Molecular Probes for Imaging Oxygen Levels in Living Cells and Hypoxic Tissues, China/Japan Young Chemists Forum: Molecular Imaging for Chemical Biology, 2014

Phosphorescence Imaging of Tumors Using Iridium Complexes, 14th International Congress of Histochemistry and Cytochemistry, 2012

2007

2014

EXCITED-STATE PROTON TRANSFER OF N-ALKYL ANILINES: EFFECT OF STRUCTURAL CHANGES OF THE AMINO GROUP, XX IUPAC Symposium on Photochemistry, 2004

Assignment of Absorption Spectra of Electrons and Holes in Nanocrystalline TiO2 Films Studied by Vis/Near-IR Transient Absorption Spectroscopy, The 6th AIST International Syposium on Photoreaction Control and Photofunctional Materials, 2003

Absorption Spectra of Electron and Hole in Nanocrystalline TiO2 Films Studied by Transient Absorption Spectroscopy, The International Nanophotonics Symposium, 2003

Intramolecular Charge Transfer with 1-(Phenyl)pyrroles, XIXth IUPAC Symposium Photochemistry, 2002

Relaxation Processes of Excited Benzoylacetone Derivatives in Solution, XXII INTERNATIONAL CONFERENCE ON PHOTOCHEMISTRY, 2005

Electron and Hole Transfer Reactions in Nanocrystalline TiO2 Films Studied by Transient Absorption Spectroscopy, XXIth International Conference on Photochemistry, 2003

EFFECTS OF SUBSTITUENT ON PHOSPHORESCENCE PROPERTIES OF CYCLOMETALATED IRIDIUM(III) COMPLEXES, XXII IUPAC Symposium on Photochemistry, 2008

EFFECTS OF SUBSTITUENT ON THE PHOSPHORESCENCE PROPERTIES OF CYCLOMETALATED IRIDIUM(III) COMPLEXES WITH 2-PHENYLPYRIDINES, XXIII IUPAC Symposium on Photochemistry, 2010

Photophysical Properties of iridium complexes bearing trimethylsilyl-substituted benzothenylpyridinato ligands, The Second International Symposium on Element Innovation, 2012

Fluorescent properties of cholesterol analogs with dansyl group in model membranes and living cells, XXIVth IUPAC Symposium on Photochemistry, 2012

Developments of hypoxia-sensing probes with high cellular uptake efficiency, The First International Symposium on Element Innovation, 2011

Quantitative Analysis of Oxygen in Living Cells and Tissues Using Iridium(III) Complexes with Cationic Substituent, 1st International Symposium of Gunma University Medical Innovation and 6th International Conference on Advanced Micro-Device Engineering, 2014

Developments of Mitochondria-Specific Oxygen Probes Based on Cationic Iridium(III) Complexes, The Fourth International Symposium on Element Innovation, 2014

Photophysical Properties of Cationic Iridium Complexes with Mitochondria Specifity and Their Intracellular Behavior, The XXVth IUPAC Symposium on Photochemistry, 2014

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Patent right, 特願2025-053902, 27 Mar. 2025

Patent right, 特願2022-069958, 21 Apr. 2022

Patent right, 特願2021-175707, 27 Oct. 2021

Patent right, 特願2021-126215, 30 Jul. 2021

Patent right, REAGENT FOR MEASURING OXYGEN CONCENTRATION IN CELL AND TISSUE, YOSHIHARA Toshitada, HIROSE Tatsuya, TOBITA Seiji, JP2020/32028, 25 Aug. 2020, GUNMA UNIVERSITY, WO 2021/039789

Patent right, REAGENT FOR FLUORESCENCE IMAGING OF LIPID DROPLETS IN CELL AND TISSUE, YOSHIHARA Toshitada, MARUYAMA Ryo, TOBITA Seiji, JP2020/12002, 18 Mar. 2020, GUNMA UNIVERSITY, WO 2020/189721

Patent right, 特願2020-211560, 11 Dec. 2020

Patent right, 特願2019-153754, 26 Aug. 2019

Patent right, 特願2019-051368, 19 Mar. 2019

Patent right, COMPOUND AND FUNTIONAL LUMINESCENT PROBE COMPRISING THE SAME, 特願09820643.6, 16 Oct. 2009, 特許EP 2 348 314 B1, 15 Feb. 2017

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Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Gunma University, Apr. 2020, Mar. 2023, Coinvestigator, 20H03618

In vivo oxygen mapping based on lifetime imaging microscopy using phosphorescent molecule, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory), Grant-in-Aid for Challenging Research (Exploratory), Gunma University, Jun. 2019, Mar. 2021, Principal investigator, 19K22947

Oxygen imaging of 3D living cells using multi-color oxygen sensing probes, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Gunma University, Apr. 2017, Mar. 2020, Principal investigator, 17H02078

Developments of a phosphorescence lifetime imaging microscopy and a probe molecule for oxygenation of retina, YOSHIHARA Toshitada, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Grant-in-Aid for Challenging Exploratory Research, Gunma University, Apr. 2015, Mar. 2017, Principal investigator, We developed a phosphorescent probe and a phosphorescence lifetime imaging microscopy to clarify an oxygen level into a blood vessel and a tissue in retina of small animals. The synthesized phosphorescence probe (DTTPH-PEG24) based on iridium complex showed the near infrared emission. The phosphorescence lifetimes of DTTPH-PEG24 which was administered to the rabbit were dependent on the oxygen partial pressure. These results that our system can visualize an oxygenation of retina of small animals., 15K12502, url

Intracellular oxygen imaging using small ratiometric oxygen probes, YOSHIHARA Toshitada, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A), Grant-in-Aid for Young Scientists (A), Gunma University, Apr. 2014, Mar. 2017, Principal investigator, We designed and synthesized ratiometric molecular probes consisting of a blue fluorescent coumarin and a red phosphorescent cationic iridium complex connected by oligo proline of oligo arginine linkers, respectively, for real time sensing of oxygen levels in living cells. The introduction of oligo arginine linkers improved dramatically the cellular uptake efficiency compared to that of oligo proline linkers. We succeed the detection of oxygen levels in living cells using our small ratiometric oxygen probes., 26702011, url

Organelle-specific intracellular oxygen measurements using iridium complex probes, TOBITA Seiji; YOSHIHARA Toshitada, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Gunma University, Apr. 2011, Mar. 2015, Coinvestigator, We synthesized new phosphorescent iridium complexes BTQ-Mito, BTQSA and BTQDM which are internalized into biological cells without any transfection reagents and show specific localization to mitochondrion, endoplasmic reticulum (ER) and lysosome, respectively. These complexes exhibit oxygen-dependent phosphorescence in living cells. Phosphorescence lifetime measurements of living HeLa cells stained with these probes under a microscope enabled us to examine the subcellular oxygen status. The phosphorescence lifetimes of these probes internalized in HeLa cells were calibrated for the oxygen partial pressure of an incubator according to Stern-Volmer analyses, providing the oxygen quenching rate constant and the average lifetime in the absence of oxygen. Using the values of the oxygen quenching rate constant and the average lifetime, the oxygen level of HeLa cells was evaluated by lifetime measurements., 23310157, url

Development of ratiometric molecular probes for monitoring oxygen levels in living cells, YOSHIHARA TOSHITADA, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, 2011, 2013, Principal investigator, Ratiometric molecular probes for the measurement of oxygen levels in lipid membranes and living cells were designed and synthesized. The synthesized molecules showed an oxygen-insensitive fluorescence with coumarin 343 and an oxygen-sensitive phosphorescence with iridium complexes. The ratio of the phosphorescence intensity for the fluorescence intensity increased with the decrease of oxygen concentration in the solution under the presence of lipid membranes. These probes were added to the cell medium and cells were incubated. The fluorescence and phosphorescence images of living cells were obtained by using microscopic techniques. These results exhibited that the ratiometric molecular probe has a high advantage for the measurements and the images of oxygen levels in living cells., 23500511, url

Phosphorescent Light-emitting lridium Complexes Serve as a Hypoxia-sensing Probe for Tumor Imaging in Living Animals., HOSAKA Masahiro; YOSHIHARA Toshitada; IIDA Yasuhiko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project), Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project), 2009, 2011, Coinvestigator, Iridium complex is a promising organic light-emitting diode material for next generation video displays that emits phosphorescence quenched by oxygen. We focused this oxygen-quenching feature for imaging tumor hypoxia. We used Red light-emitting Ir(btp)_2(acac) (BTP)to image nude mouse-transplanted tumors, and succeed to image tumors. Since BTP is easily modifiable, we synthesized BTP analogues with a longer excitation/emission wavelength. One of them, BTPHSA, depicted clear imaging from tumors transplanted 6-7 mm deep from the skin surface. Another analogue, BTPDM, shines brighter than BTP in tumors, since BTPDM has character to access easily and reach in cells. We suggest that iridium complex materials have a vast potential for imaging hypoxic lesions such as tumor tissues., 21200077, url

Developments of phosphorescence probes for oxygen concentration measurements of ex vivo and in vivo environment, YOSHIHARA Toshitada, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Gunma University, 2009, 2010, Principal investigator, The phosphorescence probes for elucidation of oxygen dynamics in lipid bilayers, cells, and tissues were developed. The synthesized iridium complexes showed high intensity phosphorescence in near-infrared region, and dissolved in water. The photochemical and photophysical properties of these complexes in solutions, lipid membranes and cells were revealed. Based on these data, the optical imaging of tumor in nude mice using these complexes was carried out, and iridium complexes have a vast potential for imaging hypoxic lesions., 21700460, url