Researcher Profile and Settings

Researcher

• Name

  MATSUNAGA Kohichi

Profile and Settings

• Name

  Matsunaga, Kohichi

Alternate Names

• Kohichi Matsunaga

Affiliation

• Gunma University

Education

• 2002, 2004, Tokyo Metropolitan University

Degree

• Doctor of Philosophy

Research Experience

• Gunma University

Research Activities

Research Areas

• Life sciences, Molecular biology

Research Interests

• Membrane traffic
• exocytosis
• insulin secretion

Research Themes

• Molecular Biology, 0135 (Japanese Only)

Published Papers

• Ubiquitination-coupled liquid phase separation regulates the accumulation of the TRIM family of ubiquitin ligases into cytoplasmic bodies., Takafumi Tozawa, Kohichi Matsunaga, Tetsuro Izumi, Naotake Shigehisa, Takamasa Uekita1, Masato Taoka, Tohru Ichimura., 2022, PLoS One, 5, 17(8), e0272700. doi: 10.1371/journal.pone.0272700., e0272700. doi: 10.1371/journal.pone.0272700.
• Cargo receptor Surf4 regulates endoplasmic reticulum export of proinsulin in pancreatic β-cells., Saegusa K, Matsunaga K, Maeda M, Saito K, Izumi T, Sato K., 2022, Commun Biol., 13, 5(1), 458, 458
• Berberine is an insulin secretagogue targeting the KCNH6 potassium channel., Miao-Miao Zhao., Jing Lu, Sen Li., Hao Wang., Xi Cao., Qi Li., Ting-Ting Shi., Kohichi Matsunaga., Chen Chen., Haixia Huang., Tetsuro Izumi., and Jin-Kui Yang., 2021, Nat. Commun., 29, 12(1), 6342, 6342
• Coixol amplifies glucose-stimulated insulin secretion via cAMP mediated signaling pathway, Hameed A; Hafizur RM; Khan MI; Jawed A; Wang H; Zhao M; Matsunaga K; Izumi T; Siddiqui S; Khan F; Adhikari A; Sharma KR, 2019, Eur J Pharmacol.
• Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa., Fan Fushun;Matsunaga Kohichi;Wang Hao;Ishizaki Ray;Kobayashi Eri;Kiyonari Hiroshi;Mukumoto Yoshiko;Okunishi Katsuhide;Izumi Tetsuro, 04 Jul. 2017, eLife, 4, 6, e26174, e26174

  DOI
• Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2., Matsunaga Kohichi;Taoka Masato;Isobe Toshiaki;Izumi Tetsuro, 07 Dec. 2016, Journal of cell science, 130, 3, 541, 550

  DOI
• PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a., Yamaoka Mami;Ando Tomomi;Terabayashi Takeshi;Okamoto Mitsuhiro;Takei Masahiro;Nishioka Tomoki;Kaibuchi Kozo;Matsunaga Kohichi;Ishizaki Ray;Izumi Tetsuro;Niki Ichiro;Ishizaki Toshimasa;Kimura Toshihide, 18 Dec. 2015, Journal of cell science, 129, 3

  DOI
• Atg14L recruits PI3 kinase to the ER for autophagosome formation, Noda, T., Matsunaga, K., Yoshimori, T, 2011, Autophagy, 7, 438, 439
• Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain., Tabata, K., Matsunaga, K., Sakane, A., Sasaki, T., Noda, T., Yoshimori, T, 2010, Mol. Biol. Cell, 21, 4162, 4172
• Autophagy requires endoplasmic reticulum-targeting of the PI3-kinase complex via Atg14L., Matsunaga, K; Morita, E; Saitoh, T; Akira, S; Ktistakis, TN; Izumi, T; Noda, T; Yoshimori, T, 2010, J. Cell. Biol., 190, 4, 511, 521
• Regulation of membrane biogenesis in autophagy via PI3P dynamics., Noda, T; Matsunaga, K; Taguchi-Atarashi, N; Yoshimori, T, 2010, Semin Cell Dev Biol.
• Modulation of topical Ptdlns3P levels by the PI phosphatase MTMR3 regulates constitutive autophagy., Taguchi-Atarashi, N; Hamasaki, M; Matsunaga, K; Omori, H; Ktistakis, TN; Yoshimori, T; Noda, T, 2010, Traffic., 11
• Atg9a controls dsDNA-driven dynamic translocation of STING and the innate immune response., Saitoh, T; Fujita, N; Hayashi, T; Takahara, K; Satoh, T; Lee, H; Matsunaga, K; Kageyama, S; Omori, H; Noda, T; Yamamoto, N; Kawai, T; Ishii, K; Takeuchi, O; Yoshimori, T; Akira, S, 2009, Proc Natl Acad Sci U S A.
• Binding Rubicon to cross the Rubicon., Matsunaga, K; Noda, T; Yoshimori, T, 2009, Autophagy, 5, 6
• Two Beclin-1 binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages., Matsunaga, K; Saitoh, T; Tabata, K; Satoh, T; Omori, H; Kurotori, N; Maejima, I; Ichimura, T; Isobe, T; Akira, S; Noda, T; Yoshimori, T, 2009, Nat. Cell. Biol., 11, 4
• Autophagy requires endoplasmic reticulum-targeting of the PI3-kinase complex via Atg14L., Matsunaga, K; Morita, E; Saitoh, T; Akira, S; Ktistakis, TN; Izumi, T; Noda, T; Yoshimori, T, 2010, J. Cell. Biol., 190, 4
• PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a, Mami Yamaoka; Tomomi Ando; Takeshi Terabayashi; Mitsuhiro Okamoto; Masahiro Takei; Tomoki Nishioka; Kozo Kaibuchi; Kohichi Matsunaga; Ray Ishizaki; Tetsuro Izumi; Ichiro Niki; Toshimasa Ishizaki; Toshihide Kimura, Feb. 2016, JOURNAL OF CELL SCIENCE, 129, 3, 637, 649, Scientific journal

  DOI
• Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa, Fushun Fan; Kohichi Matsunaga; Hao Wang; Ray Ishizaki; Eri Kobayashi; Hiroshi Kiyonari; Yoshiko Mukumoto; Katsuhide Okunishi; Tetsuro Izumi, Jul. 2017, ELIFE, 6, Scientific journal

  DOI
• Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2, Kohichi Matsunaga; Masato Taoka; Toshiaki Isobe; Tetsuro Izumi, Feb. 2017, JOURNAL OF CELL SCIENCE, 130, 3, 541, 550, Scientific journal

  DOI
• 哺乳類オートファジー研究の夜明け, 藤田尚信; 松永耕一; 野田健司; 吉森保, 2008
• オートファジーの駆動・制御機構の進化と疾患との関わり, 松永耕一; 藤田尚信; 吉森保, 2009
• Rab GTPaseによるインスリン細胞内物流システムの制御, 松永 耕一, 2013, 28
• Rab GTPaseによるインスリン顆粒の成熟と分泌の制御機構, 松永 耕一, 2014, 28
• Two Beclin-1 binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages., Matsunaga, K; Saitoh, T; Tabata, K; Satoh, T; Omori, H; Kurotori, N; Maejima, I; Ichimura, T; Isobe, T; Akira, S; Noda, T; Yoshimori, T, 2009, Nat. Cell. Biol., 11, 4
• Binding Rubicon to cross the Rubicon., Matsunaga, K; Noda, T; Yoshimori, T, 2009, Autophagy, 5, 6
• Atg9a controls dsDNA-driven dynamic translocation of STING and the innate immune response., Saitoh, T; Fujita, N; Hayashi, T; Takahara, K; Satoh, T; Lee, H; Matsunaga, K; Kageyama, S; Omori, H; Noda, T; Yamamoto, N; Kawai, T; Ishii, K; Takeuchi, O; Yoshimori, T; Akira, S, 2009, Proc Natl Acad Sci U S A.
• Modulation of topical Ptdlns3P levels by the PI phosphatase MTMR3 regulates constitutive autophagy., Taguchi-Atarashi, N; Hamasaki, M; Matsunaga, K; Omori, H; Ktistakis, TN; Yoshimori, T; Noda, T, 2010, Traffic., 11
• Regulation of membrane biogenesis in autophagy via PI3P dynamics., Noda, T; Matsunaga, K; Taguchi-Atarashi, N; Yoshimori, T, 2010, Semin Cell Dev Biol.
• Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain., Tabata, K; Matsunaga, K; Sakane, A; Sasaki, T; Noda, T; Yoshimori, T, 2011, Mol. Biol. Cell.
• Atg14L recruits PI3 kinase to the ER for autophagosome formation., Noda, T; Matsunaga, K; Yoshimori, T, 2011, Autophagy
• PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a, Yamaoka, Mami;Ando, Tomomi;Terabayashi, Takeshi;Okamoto, Mitsuhiro;Takei, Masahiro;Nishioka, Tomoki;Kaibuchi, Kozo;Matsunaga, Kohichi;Ishizaki, Ray;Izumi, Tetsuro;Niki, Ichiro;Ishizaki, Toshimasa;Kimura, Toshihide, 2016, JOURNAL OF CELL SCIENCE, 129, 3, 637, 649

  DOI
• Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2, Matsunaga, Kohichi;Taoka, Masato;Isobe, Toshiaki;Izumi, Tetsuro, 2017, JOURNAL OF CELL SCIENCE, 130, 3, 541, 550

  DOI
• Rab27エフェクターExophilin8によるインスリン分泌制御機構, ;;, Apr. 2016, 59, Suppl.1, S
• 新規インスリン分泌促進化合物の分子機構の解明, ;;;;;, Apr. 2018, 61, Suppl.1, S
• リポタンパク質の分泌を制御するSFT-4/Surf4ファミリータンパク質の発見とその機能解析, ;;;;;;, Apr. 2019, 95, 1, 334
• Atg14L recruits PtdIns 3-kinase to the ER for autophagosome formation, Noda, Takeshi;Matsunaga, Kohichi;Yoshimori, Tamotsu, 2011, AUTOPHAGY, 7, 4, 438, 439

  DOI
• Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa, Fan, Fushun;Matsunaga, Kohichi;Wang, Hao;Ishizaki, Ray;Kobayashi, Eri;Kiyonari, Hiroshi;Mukumoto, Yoshiko;Okunishi, Katsuhide;Izumi, Tetsuro, 2017, ELIFE, 6

  DOI
• Berberine is an insulin secretagogue targeting the KCNH6 potassium channel, Zhao, Miao-Miao;Lu, Jing;Li, Sen;Wang, Hao;Cao, Xi;Li, Qi;Shi, Ting-Ting;Matsunaga, Kohichi;Chen, Chen;Huang, Haixia;Izumi, Testuro;Yang, Jin-Kui, 2021, NATURE COMMUNICATIONS, 12, 1

  DOI
• Ubiquitination-coupled liquid phase separation regulates the accumulation of the TRIM family of ubiquitin ligases into cytoplasmic bodies, Tozawa, Takafumi;Matsunaga, Kohichi;Izumi, Tetsuro;Shigehisa, Naotake;Uekita, Takamasa;Taoka, Masato;Ichimura, Tohru, 2022, PLOS ONE, 17, 8

  DOI
• 膵β細胞においてSFT-4/Surf4は新生プロインスリンの小胞体からの輸送を制御する, ;;;, Aug. 2020, 96, 1, 294
• 膵β細胞においてSFT-4/Surf4は新生プロインスリンの小胞体からの輸送を制御する, ;;;;;, Jun. 2020, 72回, AW
• Berberine is an insulin secretagogue targeting the KCNH6 potassium channel., Zhao Miao-Miao;Lu Jing;Li Sen;Wang Hao;Cao Xi;Li Qi;Shi Ting-Ting;Matsunaga Kohichi;Chen Chen;Huang Haixia;Izumi Tetsuro;Yang Jin-Kui, 23 Sep. 2021, Nature communications, 12, 1

  DOI
• Author Correction: Berberine is an insulin secretagogue targeting the KCNH6 potassium channel., Zhao Miao-Miao;Lu Jing;Li Sen;Wang Hao;Cao Xi;Li Qi;Shi Ting-Ting;Matsunaga Kohichi;Chen Chen;Huang Haixia;Izumi Tetsuro;Yang Jin-Kui, 29 Oct. 2021, Nature communications, 12, 1

  DOI
• Cargo receptor Surf4 regulates endoplasmic reticulum export of proinsulin in pancreatic β-cells., Saegusa Keiko;Matsunaga Kohichi;Maeda Miharu;Saito Kota;Izumi Tetsuro;Sato Ken, 13 May 2022, Communications biology, 5, 1

  DOI
• インスリン分泌経路における積み荷受容体SFT-4/Surf4の機能解析, ;;;;;, Jun. 2021, 73回, WS22
• 複数のRab27エフェクターExophilinsが制御する、インスリン顆粒開口放出機構, ;;;;;, Apr. 2022, 65, Suppl.1, S

MISC

• Atg9a controls dsDNA-driven dynamic translocation of STING and the innate immune response, Tatsuya Saitoh; Naonobu Fujita; Takuya Hayashi; Keigo Takahara; Takashi Satoh; Hanna Lee; Kohichi Matsunaga; Shun Kageyama; Hiroko Omori; Takeshi Noda; Naoki Yamamoto; Taro Kawai; Ken Ishii; Osamu Takeuchi; Tamotsu Yoshimori; Shizuo Akira, Dec. 2009, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106, 49, 20842, 20846

  DOI
• Atg9a controls dsDNA-driven dynamic translocation of STING and the innate immune response, Tatsuya Saitoh; Naonobu Fujita; Takuya Hayashi; Keigo Takahara; Takashi Satoh; Hanna Lee; Kohichi Matsunaga; Shun Kageyama; Hiroko Omori; Takeshi Noda; Naoki Yamamoto; Taro Kawai; Ken Ishii; Osamu Takeuchi; Tamotsu Yoshimori; Shizuo Akira, Dec. 2009, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106, 49, 20842, 20846

  DOI
• Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L, Kohichi Matsunaga; Eiji Morita; Tatsuya Saitoh; Shizuo Akira; Nicholas T. Ktistakis; Tetsuro Izumi; Takeshi Noda; Tamotsu Yoshimori, Aug. 2010, JOURNAL OF CELL BIOLOGY, 190, 4, 511, 521

  DOI
• Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L, Kohichi Matsunaga; Eiji Morita; Tatsuya Saitoh; Shizuo Akira; Nicholas T. Ktistakis; Tetsuro Izumi; Takeshi Noda; Tamotsu Yoshimori, Aug. 2010, JOURNAL OF CELL BIOLOGY, 190, 4, 511, 521

  DOI
• Rubicon and PLEKHM1 Negatively Regulate the Endocytic/Autophagic Pathway via a Novel Rab7-binding Domain, Keisuke Tabata; Kohichi Matsunaga; Ayuko Sakane; Takuya Sasaki; Takeshi Noda; Tamotsu Yoshimori, Dec. 2010, MOLECULAR BIOLOGY OF THE CELL, 21, 23, 4162, 4172

  DOI
• Regulation of membrane biogenesis in autophagy via PI3P dynamics, Takeshi Noda; Kohichi Matsunaga; Naoko Taguchi-Atarashi; Tamotsu Yoshimori, Sep. 2010, SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 21, 7, 671, 676, Book review

  DOI
• Regulation of membrane biogenesis in autophagy via PI3P dynamics, Takeshi Noda; Kohichi Matsunaga; Naoko Taguchi-Atarashi; Tamotsu Yoshimori, Sep. 2010, SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 21, 7, 671, 676, Book review

  DOI
• Modulation of Local PtdIns3P Levels by the PI Phosphatase MTMR3 Regulates Constitutive Autophagy, Naoko Taguchi-Atarashi; Maho Hamasaki; Kohichi Matsunaga; Hiroko Omori; Nicholas T. Ktistakis; Tamotsu Yoshimori; Takeshi Noda, Apr. 2010, TRAFFIC, 11, 4, 468, 478

  DOI
• Modulation of Local PtdIns3P Levels by the PI Phosphatase MTMR3 Regulates Constitutive Autophagy, Naoko Taguchi-Atarashi; Maho Hamasaki; Kohichi Matsunaga; Hiroko Omori; Nicholas T. Ktistakis; Tamotsu Yoshimori; Takeshi Noda, Apr. 2010, TRAFFIC, 11, 4, 468, 478

  DOI
• PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a, Mami Yamaoka; Tomomi Ando; Takeshi Terabayashi; Mitsuhiro Okamoto; Masahiro Takei; Tomoki Nishioka; Kozo Kaibuchi; Kohichi Matsunaga; Ray Ishizaki; Tetsuro Izumi; Ichiro Niki; Toshimasa Ishizaki; Toshihide Kimura, Feb. 2016, JOURNAL OF CELL SCIENCE, 129, 3, 637, 649

  DOI
• PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a, Mami Yamaoka; Tomomi Ando; Takeshi Terabayashi; Mitsuhiro Okamoto; Masahiro Takei; Tomoki Nishioka; Kozo Kaibuchi; Kohichi Matsunaga; Ray Ishizaki; Tetsuro Izumi; Ichiro Niki; Toshimasa Ishizaki; Toshihide Kimura, Feb. 2016, JOURNAL OF CELL SCIENCE, 129, 3, 637, 649

  DOI
• Atg14L recruits PtdIns 3-kinase to the ER for autophagosome formation, Takeshi Noda; Kohichi Matsunaga; Tamotsu Yoshimori, Apr. 2011, AUTOPHAGY, 7, 4, 438, 439, Others

  DOI
• Atg14L recruits PtdIns 3-kinase to the ER for autophagosome formation, Takeshi Noda; Kohichi Matsunaga; Tamotsu Yoshimori, Apr. 2011, AUTOPHAGY, 7, 4, 438, 439, Others

  DOI
• Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa, Fushun Fan; Kohichi Matsunaga; Hao Wang; Ray Ishizaki; Eri Kobayashi; Hiroshi Kiyonari; Yoshiko Mukumoto; Katsuhide Okunishi; Tetsuro Izumi, Jul. 2017, ELIFE, 6, e26174

  DOI
• Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa, Fushun Fan; Kohichi Matsunaga; Hao Wang; Ray Ishizaki; Eri Kobayashi; Hiroshi Kiyonari; Yoshiko Mukumoto; Katsuhide Okunishi; Tetsuro Izumi, Jul. 2017, ELIFE, 6, e26174

  DOI
• 【メンブレントラフィックの奔流 分子から細胞、そして個体へ】メンブレントラフィックの分子メカニズム 哺乳類オートファジー研究の夜明け, 藤田 尚信; 松永 耕一; 野田 健司; 吉森 保, Dec. 2008, 蛋白質・核酸・酵素, 53, 16, 2106, 2110
• Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2, Kohichi Matsunaga; Masato Taoka; Toshiaki Isobe; Tetsuro Izumi, Feb. 2017, JOURNAL OF CELL SCIENCE, 130, 3, 541, 550

  DOI
• 膵B細胞におけるエンドサイトーシスの時間的・空間的制御機構の解析, 山岡 真美; 安藤 朋海; 寺林 健; 松永 耕一; 泉 哲郎; 仁木 一郎; 石崎 敏理; 木村 俊秀, Apr. 2015, 糖尿病, 58, Suppl.1, S, 441
• 膵B細胞におけるRab27a-GAP新規結合タンパク質の機能解析, 山岡 真美; 岡本 光弘; 安藤 朋海; 寺林 健; 松永 耕一; 泉 哲郎; 仁木 一郎; 石崎 敏理; 木村 俊秀, Apr. 2014, 糖尿病, 57, Suppl.1, S, 405
• 膵B細胞におけるエンドサイトーシスの時間的・空間的制御機構の解析, 山岡 真美; 寺林 健; 松永 耕一; 泉 哲郎; 仁木 一郎; 石崎 敏理; 木村 俊秀, Dec. 2015, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 88回・38回, [2P0046], [2P0046]
• Two Beclin-1 binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages., Matsunaga, K; Saitoh, T; Tabata, K; Satoh, T; Omori, H; Kurotori, N; Maejima, I; Ichimura, T; Isobe, T; Akira, S; Noda, T; Yoshimori, T, 2009, 11, 4, 385, 96
• Binding Rubicon to cross the Rubicon., Matsunaga, K; Noda, T; Yoshimori, T, 2009, 5, 6, 876, 877
• Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain., Tabata, K; Matsunaga, K; Sakane, A; Sasaki, T; Noda, T; Yoshimori, T, 2011

  DOI
• 哺乳類オートファジー研究の夜明け, 藤田尚信; 松永耕一; 野田健司; 吉森保, 2008
• オートファジーの駆動・制御機構の進化と疾患との関わり, 松永耕一; 藤田尚信; 吉森保, 2009
• Rab GTPaseによるインスリン顆粒の成熟と分泌の制御機構, 松永 耕一, 2014, 28, 1-6.
• Rab GTPaseによるインスリン細胞内物流システムの制御, 松永 耕一, 2013, 28, 103, 108
• Two Beclin-1 binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages., Matsunaga, K; Saitoh, T; Tabata, K; Satoh, T; Omori, H; Kurotori, N; Maejima, I; Ichimura, T; Isobe, T; Akira, S; Noda, T; Yoshimori, T, 2009, Nat. Cell. Biol., 11, 4, 385, 96
• Binding Rubicon to cross the Rubicon., Matsunaga, K; Noda, T; Yoshimori, T, 2009, Autophagy, 5, 6, 876, 877
• Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2, Kohichi Matsunaga; Masato Taoka; Toshiaki Isobe; Tetsuro Izumi, 2017, Journal of Cell Science, 130, 3, 541, 550

  DOI

Books etc

• 膵β細胞におけるエキソサイトーシスとGTP結合蛋白質, 2012

Presentations

• メンブレントラフィックによる細胞内物流システムの分子機構, 松永耕一, 防衛大学校 GSコロキアム「感染症と安全保障 PartIV」, 29 Oct. 2019, 29 Oct. 2019, 29 Oct. 2019, Japanese
• 二つの新規Beclin 1 結合タンパク質であるAtg14L とRubicon は、それぞれオートファジーを正負に制御する, 日本生化学会, 2009
• Roles of the Rab27 effector, Exophilin8/MyRIP, in insulin granule exocytosis., The 2nd IMCR symposium on endocrine and metabolism: International Frontiers in Homeostatic Regulation Research in Gunma Univ., 2016
• 第８２回日本生化学会大会シンポジウム 変貌するオルガネラ像～ダイナミクスと高次機能～ シンポジスト：「二つの新規Beclin 1 結合タンパク質であるAtg14L とRubicon は、それぞれオートファジーを正負に制御する」, 2009
• 肥満・脂質代謝におけるGranuphilinたんぱく質の役割 第２８回日本糖尿病・肥満動物学会年次学術集会 一般演題, 2014
• Functional cross-talk between Rab27a and Rab2a via their dual effector is required for insulin guranule maturation and secretion in pancreatic beta cells, 2015
• Functional cross-talk between Rab27a and Rab2a through their dual effector is required for insulin guranule maturation and secretion in pancreatic beta cells. International Symposium on Homeostasis Through Development, Life and Diseases in Gunma Univ, 2014
• Rab27エフェクターExophilin8によるインスリン分泌制御機構 第５９回日本糖尿病学会年次学術集会（京都）, 2016
• インスリン分泌制御におけるExophilin8たんぱく質の機能解析 第６2回北関東医学会総会 ポスター発表, 2015
• Exophilin8遺伝子改変マウスを用いたインスリン分泌制御機構の解析 第６１回北関東医学会総会 ポスター発表, 2014
• 膵B細胞におけるRab27a-GAP新規結合タンパク質の機能解析、 第57回日本糖尿病学会年次学術集会（大阪）, 2014
• 膵B細胞におけるエンドサイトーシスの時間的・空間的制御機構の解析、BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会）, 2015
• Rab27エフェクターExophilin8ノックアウトマウスのインスリン分泌制御における解析 第３０回日本糖尿病・肥満動物学会年次学術集会 一般演題, 2016
• ケミカルライブラリーを用いた網羅的な新規インスリン分泌制御化合物の探索, 2013
• Roles of the Rab27 effector, Exophilin8/MyRIP, in insulin granule exocytosis., The 2nd IMCR symposium on endocrine and metabolism: International Frontiers in Homeostatic Regulation Research in Gunma Univ., 2016
• Functional cross-talk between Rab27a and Rab2a via their dual effector is required for insulin guranule maturation and secretion in pancreatic beta cells, 2015
• Functional cross-talk between Rab27a and Rab2a through their dual effector is required for insulin guranule maturation and secretion in pancreatic beta cells. International Symposium on Homeostasis Through Development, Life and Diseases in Gunma Univ, 2014
• Exophilin-8/MyRIP/Slac2C assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa 第７０回日本細胞生物学会・第５０回日本発生生物学会合同大会, 第７０回日本細胞生物学会・第５０回日本発生生物学会合同大会, 05 Jun. 2018
• 新規インスリン分泌促進化合物の分子機構の解明 第６１回日本糖尿病学会年次学術集会, 第６１回日本糖尿病学会年次学術集会, 24 May 2018
• Exophilin-8/MyRIP/Slac2C assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa 第７０回日本細胞生物学会・第５０回日本発生生物学会合同大会 ポスター, 第７０回日本細胞生物学会・第５０回日本発生生物学会合同大会 ポスター, 05 Jun. 2018

Awards

• 2009

Research Projects

• 2017, Principal investigator
• 2014
• 2013, Principal investigator
• 2012
• 2012, Principal investigator
• 2012, Principal investigator
• 2011, Principal investigator
• 2011, Principal investigator
• 2011, Principal investigator
• 2011, Principal investigator
• Homeostasis of the epidermal cells and its dysfunction in epidermolysis bullosa, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Gunma University, Apr. 2019, Mar. 2022, 19H03449
• Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, Apr. 2018, Mar. 2021, 18K06950
• Molecular mechanism of insulin granules exocytosis via the novel Exophilin-8 complexes., Matsunaga Kohichi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, Apr. 2015, Mar. 2018, Insulin is produced by beta-cells and is stored as secretory granules in beta-cells of the pancreatic islets. Secretion of insulin requires a series of membrane dynamics, namely, producing granules from the Golgi apparatus, sorting to granules and maturation, and trafficking and fusion to the plasma membrane. Secretory granules transfer to the actin cortex near the plasma membrane before they fuse with plasma membrane. In the present study, we showed that Exophilin-8-knockout mice showed significantly higher blood glucose level, and Exophilin-8-null pancreatic beta-cells exhibited decreased insulin secretion in glucose stimulation and lost insulin granule location at the F-actin rich cell periphery. Furthermore, we found that Exophilin-8 formed the complex with RIM-BP2 and Myosin-VIIa, and the complex had important role for the granule localization to F-actin rich cell periphery., 15K08295
• Identification of activator of glucose-stimulated insulin secretion by high-throughput chemical screening, Ushigome Takeshi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Grant-in-Aid for Challenging Exploratory Research, Gunma University, Apr. 2014, Mar. 2017, Over 400 million people had diabetes mellitus in the world, and new drug development is important for the reason. We established a high-throughput screening assay system for the identification of chemical compounds that enhance (or inhibit) insulin secretion. In consequence, we identified six effective chemicals. To investigate molecular mechanism of these chemical compound activated (or inhibited) insulin secretion, we performed comprehensive binding assay with these chemicals in INS1 832/13 cells. we identified mitochondrial protein as the one of activator of chemical. Therefore, we obtained basic data to develop a new drug for diabetes., 26670151
• Role of Rab GTPases in the intracellular logistics of insulin granule, MATSUNAGA Kohichi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Gunma University, Apr. 2013, Mar. 2015, Regulated secretion is a main pathway for secretory cells to deliver bioactive molecules to the surface or outside of the cell. Although the molecular machinery for these processes have been characterized, precise molecular mechanisms are poorly understood. Previous studies have shown that the small GTPase Rab27 is required for late steps of this pathway, granule trafficking, docking, and fusion of insulin granule. We found that Rab27a forms a novel protein complex in pancreatic beta-cells. Although Rab27a localized on almost all granules, the complex specifically localized on immature granules. Furthermore, knockdown of Rab27a and that of other component of the complex decreased glucose-induced insulin secretion and conversion of proinsulin to insulin. These data suggest that the novel Rab27a complex is involved in the transition from granule maturation to secretion in the regulated secretory pathway., 25860208
• Molecular basis of Rab27 GTPase-mediated regulated exocytosis, MATSUNAGA Kohichi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Gunma University, 2011, 2012, To investigate the molecular mechanism of Rab27 GTPase-mediated regulated exocytosis, I performed comprehensive proteomic analysis of Rab27a binding proteins in various secreting cells. I identified dozens of novel Rab27a binding proteins and also identified cell strain specific binding proteins. Therefore, I obtained basic data to clarify the molecular mechanism for regulating Rab27-mediated exocytosis pathway., 23790354