Researcher Profile and Settings

Researcher

• Name

  TAKAHASHI Tsuyoshi

Affiliation

• Division of Materials and Environment（Department of Applied Chemistry）, Associate Professor

Profile and Settings

• Name

  Takahashi, Tsuyoshi

Alternate Names

• Tsuyoshi Takahashi

Affiliation

• Gunma University, Associate Professor
• Gunma University

Affiliation

• Affiliation

  Graduate School of Science and Technology

Education

• 1996, Tokyo Institute of Technology, School of Bioscience and Biotechnology

Degree

• Doctor Engineering
• Doctor Engineering

Association Memberships

• Japanese Peptide Society
• The Chemical Sociey of Japan

Research Experience

• Gunma University
• Former Institution / Organization Tokyo Institute of Technology Graduate School of Biosciences and Biotechnology Department of Bioengineering, Assistant Professor, Assistant prof lv

Research Activities

Published Papers

• Selection of MDM2-binding peptides in Escherichia coli using an engineered split intein and aminoglycoside phosphotransferase, Yutaro Watanabe, Chiori Tezuka, Kana Nobusawa, Tsuyoshi Takahashi, Mar. 2025, Chemical Communications, 61, 6332, 6335, Scientific journal
• Sugar-mediated non-canonical ubiquitination impairs Nrf1/NFE2L1 activation, Yukiko Yoshida; Tsuyoshi Takahashi; Nozomi Ishii; Ichiro Matsuo; Satoshi Takahashi; Haruka Inoue; Akinori Endo; Hikaru Tsuchiya; Meari Okada; Chikara Ando; Takehiro Suzuki; Naoshi Dohmae; Yasushi Saeki; Keiji Tanaka; Tadashi Suzuki, Aug. 2024, Molecular Cell, 84, 16, 3115, 3127, Scientific journal

  DOI
• Luminescence detection of peptide:N-glycanase activity using engineered split inteins., Tsuyoshi Takahashi; Tatsuya Uchibayashi; Nozomi Ishii; Ichiro Matsuo; Yukiko Yoshida; Tadashi Suzuki, 29 Nov. 2022, Chemical communications (Cambridge, England), 58, 95, 13282, 13285, Scientific journal

  DOI
• Detecting ligand–protein interactions inside cells using reactive peptide tags and split luciferase, Tsuyoshi Takahashi; Hiroaki Hagiwara, 02 Sep. 2021, Chemical Communications, 57, 9906, 9909, Scientific journal

  DOI
• Activation of Protease and Luciferase Using Engineered Nostoc Punctiforme PCC73102 DnaE Intein with Altered Split Position, Misaki Kawase; Meiko Fujioka; Tsuyoshi Takahashi, 02 Feb. 2021, ChemBioChem, 22, 3, 577, 584, Scientific journal

  DOI
• Affinity Control of Monosaccharide Conjugated Peptides against Lectins with a Set of Amino Acid Substitutions on α-Helical Structures, Yusuke Maeda; Toshiki Sawada; Tsuyoshi Takahashi; Hideya Yuasa; Hisakazu Mihara, 18 Nov. 2020, Bioconjugate Chemistry, 31, 11, 2533, 2540, Scientific journal

  DOI
• Generation of Active Protease Depending on Peptide-Protein Interactions Using Interaction-Dependent Native Chemical Ligation and Protein Trans-Splicing, Tsuyoshi Takahashi, 15 Oct. 2019, Bulletin of the Chemical Society of Japan, 92, 10, 1767, 1772, Scientific journal

  DOI
• Inhibiting Aggregation of β-Amyloid by Folded and Unfolded Forms of Fimbrial Protein of Gram-Negative Bacteria, Keisuke Yamamoto; Misa Oyaizu; Tsuyoshi Takahashi; Yoshihito Watanabe; Osami Shoji, 2017, ChemistrySelect, 2, 28, 9058, 9062, Scientific journal

  DOI
• Interaction–dependent native chemical ligation and protein trans–splicing (IDNCL-PTS) for detection and visualization of ligand–protein interactions, Tsuyoshi Takahashi; Akinori Saito, 2016, ChemistrySelect, 1, 8, 1768, 1772, Scientific journal

  DOI
• Construction of sensor protein that responses to amyloid β-peptide oligomers and demonstration of screening capabilities for oligomer inhibitors, Tsuyoshi Takahashi, 2015, Chemistry Letters, 44, 1, 67, 69, Scientific journal

  DOI
• Interaction-dependent native chemical ligation and enzyme reconstitution for detection of peptide-peptide interaction, Tsuyoshi Takahashi; Akinori Saito, 2014, Chemistry Letters, 43, 8, 1357, 1359, Scientific journal

  DOI
• Construction of proteins with molecular recognition capabilities using α3β3 de novo protein scaffolds, Hiromichi Okura; Hisakazu Mihara; Tsuyoshi Takahashi, Oct. 2013, Protein Engineering, Design and Selection, 26, 10, 705, 711, Scientific journal

  DOI
• Modification of a small β-barrel protein, to give pseudo-amyloid structures, inhibits amyloid β-peptide aggregation, Yuko Murakoshi; Tsuyoshi Takahashi; Hisakazu Mihara, 02 Apr. 2013, Chemistry - A European Journal, 19, 14, 4525, 4531, Scientific journal

  DOI
• アルツハイマー病アミロイドβを標的とした分子設計, TAKAHASHI Tsuyoshi, Mar. 2013, 66, 3, 216, 217
• Gold nanoparticles conjugated with monosaccharide-modified peptide for lectin detection, Hiroshi Tsutsumi; Hiroyuki Ohkusa; Hyejin Park; Tsuyoshi Takahashi; Hideya Yuasa; Hisakazu Mihara, 15 Nov. 2012, Bioorganic and Medicinal Chemistry Letters, 22, 22, 6825, 6827, Scientific journal

  DOI
• Effects of Group 3 LEA protein model peptides on desiccation-induced protein aggregation, Takao Furuki; Tempei Shimizu; Sohini Chakrabortee; Kentarou Yamakawa; Rie Hatanaka; Tsuyoshi Takahashi; Takahiro Kikawada; Takashi Okuda; Hisakazu Mihara; Alan Tunnacliffe; Minoru Sakurai, Jul. 2012, Biochimica et Biophysica Acta - Proteins and Proteomics, 1824, 7, 891, 897, Scientific journal

  DOI
• Cell-adhesive hydrogels composed of peptide nanofibers responsive to biological ions, Toshiki Sawada; Masaki Tsuchiya; Tsuyoshi Takahashi; Hiroshi Tsutsumi; Hisakazu Mihara, Jun. 2012, Polymer Journal, 44, 6, 651, 657, Scientific journal

  DOI
• FRET detection of amyloid β-peptide oligomerization using a fluorescent protein probe presenting a pseudo-amyloid structure, Tsuyoshi Takahashi; Hisakazu Mihara, 01 Feb. 2012, Chemical Communications, 48, 10, 1568, 1570, Scientific journal

  DOI
• Terminal sequence importance of de novo proteins from binary-patterned library: Stable artificial proteins with 11- or 12-amino acid alphabet, Hiromichi Okura; Tsuyoshi Takahashi; Hisakazu Mihara, 2012, Protein and Peptide Letters, 19, 6, 673, 679, Scientific journal

  DOI
• Anti-aggregation Effects on Proteins by 22-mer Model Peptides of Group3 LEA Proteins, FURUKI TAKAO; SHIMIZU TEMPEI; CHARKRABORTEE SOHINI; HATANAKA RIE; TAKAHASHI TSUYOSHI; KIKAWADA TAKAHIRO; OKUDA TAKASHI; MIHARA HISAKAZU; TUNNACLIFFE ALAN; SAKURAI MINORU, 15 Oct. 2011, 低温生物工学会誌, 57, 2, 139, 142

  DOI
• A novel β-loop scaffold of phage-displayed peptides for highly specific affinities, Toshiki Sawada; Kojiro Ishiguro; Tsuyoshi Takahashi; Hisakazu Mihara, 01 Sep. 2011, Molecular BioSystems, 7, 9, 2558, 2562, Scientific journal

  DOI
• A novel peptide array using a phage display system for protein detection, Toshiki Sawada; Kojiro Ishiguro; Tsuyoshi Takahashi; Hisakazu Mihara, 2011, Chemistry Letters, 40, 5, 508, 509, Scientific journal

  DOI
• Designed short peptides that form amyloid-like fibrils in coassembly with amyloid β-peptide (Aβ) decrease the toxicity of Aβ to neuronal PC12 cells, Miho Suzuki; Tsuyoshi Takahashi; Junichi Sato; Masayasu Mie; Eiry Kobatake; Hisakazu Mihara, 26 Jul. 2010, ChemBioChem, 11, 11, 1525, 1530, Scientific journal

  DOI
• Peptide nanofibers modified with a protein by using designed anchor molecules bearing hydrophobic and functional moieties, Ayaka Miyachi; Tsuyoshi Takahashi; Sachiko Matsumura; Hisakazu Minara, 11 Jun. 2010, Chemistry - A European Journal, 16, 22, 6644, 6650, Scientific journal

  DOI
• Model Study of the Desiccation-induced Vitrification of Group-3 Late Embrogenesis Abundant Proteins, FURUKI Takao; SHIMIZU Tempei; TAKAHASHI Tsuyoshi; MIHARA Hisakazu; KIKAWADA Takahiro; OKUDA Takashi; SAKURAI Minoru, 15 Mar. 2010, Cryobiology and cryotechnology, 56, 1, 27, 30

  DOI
• Desiccation-induced structuralization and glass formation of group 3 late embryogenesis abundant protein model peptides, Tempei Shimizu; Yasushi Kanamori; Takao Furuki; Takahiro Kikawada; Takashi Okuda; Tsuyoshi Takahashi; Hisakazu Mihara; Minoru Sakurai, 16 Feb. 2010, Biochemistry, 49, 6, 1093, 1104, Scientific journal

  DOI
• Rational design of amyloid beta peptide-binding proteins: Pseudo-A beta beta-sheet surface presented in green fluorescent protein binds tightly and preferentially to structured A beta, Tsuyoshi Takahashi; Kenichi Ohta; Hisakazu Mihara, Feb. 2010, PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 78, 2, 336, 347, Scientific journal

  DOI
• Design and Conformational Analysis of Natively Folded beta-Hairpin Peptides Stabilized by Nucleobase Interactions, Ryuichi Uozumi; Tsuyoshi Takahashi; Toshimasa Yamazaki; Viktor Granholm; Hisakazu Mihara, 2010, BIOPOLYMERS, 94, 6, 830, 842, Scientific journal

  DOI
• Affinity-based screening of peptides recognizing assembly states of self-assembling peptide nanomaterials, Toshiki Sawada; Tsuyoshi Takahashi; Hisakazu Mihara, 14 Oct. 2009, Journal of the American Chemical Society, 131, 40, 14434, 14441, Scientific journal

  DOI
• Selection and structural analysis of de novo proteins from an alpha 3 beta 3 genetic library, Mariejoy Therese Jumawid; Tsuyoshi Takahashi; Toshimasa Yamazaki; Hiroshi Ashigai; Hisakazu Mihara, Feb. 2009, PROTEIN SCIENCE, 18, 2, 384, 398, Scientific journal

  DOI
• RNA aptamers selected against amyloid β-peptide (Aβ) inhibit the aggregation of Aβ, Tsuyoshi Takahashi; Kosuke Tada; Hisakazu Mihara, 2009, Molecular BioSystems, 5, 9, 986, 991, Scientific journal

  DOI
• The Effect of Model Peptides for Group-3 Late Embryogenesis Abundant (G3LEA) Proteins on Protein Aggregation, SHIMIZU Tempei; FURUKI Takao; KANAMORI Yasushi; KIKAWADA Takahiro; OKUDA Takashi; TAKAHASHI Tsuyoshi; MIHARA Hisakazu; CHAKRABORTEED Sohini; TUNNACLIFFE Alan; SAKURAI Minoru, 25 Oct. 2008, 生物物理, 48, Supplement 1, S31

  DOI
• Peptide and Protein Mimetics Inhibiting Amyloid beta-Peptide Aggregation, Tsuyoshi Takahashi; Hisakazu Mihara, Oct. 2008, ACCOUNTS OF CHEMICAL RESEARCH, 41, 10, 1309, 1318

  DOI
• Construction of beta-sheet proteins embedding amyloid beta peptide (A beta) sequences and inhibitory activity of A beta aggregation, Yuko Murakoshi; Tsuyoshi Takahashi; Hisakazu Mihara, Aug. 2008, JOURNAL OF PEPTIDE SCIENCE, 14, 8, 133, 133
• Screening of peptides that specifically bind to fine structured peptide nanofibers, Sawada Toshiki; Takahashi Tsuyoshi; Mihara Hisakazu, 2008, Journal of Peptide Science, 14, 8, 185, 186
• Model study of the Desiccation-Induced Structural Transformations of Group-3 Late Embryogenesis Abundant (G3LEA) Proteins, SHIMIZU Tempei; KANAMORI Yasushi; FURUKI Takao; OKUDA Takashi; KIKAWADA Takahiro; TAKAHASHI Tsuyoshi; MIHARA Hisakazu; SAKURAI Minoru, 30 Dec. 2007, 低温生物工学会誌, 53, 2, 101-105, 105

  DOI
• 3P024 Model study of the desiccation-induced structural transformations of Group-3 Late Embryogenesis Abundant (G3LEA) proteins(Hemeproteins. Electronic states. Proteins-structure and structure-function relationship,Oral Presentations), Shimizu Tempei; Miyama Shohei; Furuki Takao; Kanamori Yasushi; Kikawada Takahiro; Okuda Takashi; Takahashi Tsuyoshi; Mihara Hisakazu; Sakurai Minoru, 20 Nov. 2007, Biophysics, 47, 1, S209

  DOI
• Embedding the amyloid beta-pepticle sequence in green fluorescent protein inhibits A beta oligomerization, Tsuyoshi Takahashi; Kenichi Ohta; Hisakazu Mihara, Jun. 2007, CHEMBIOCHEM, 8, 9, 985, 988, Scientific journal

  DOI
• Interactions between peptides containing nucleobase amino acids and T7 phages displaying S. cerevisiae proteins, Sinya Watanabe; Kin-Ya Tomizaki; Tsuyoshi Takahashi; Kenji Usui; Kotaro Kajikawa; Hisakazu Mihara, 2007, Biopolymers - Peptide Science Section, 88, 2, 131, 140, International conference proceedings

  DOI
• Design of peptides that form amyloid-like fibrils capturing amyloid β1-42 peptides, Junichi Sato; Tsuyoshi Takahashi; Hideo Oshima; Sachiko Matsumura; Hisakazu Mihara, 2007, Chemistry - A European Journal, 13, 27, 7745, 7752, Scientific journal

  DOI
• Utilization of L-α-nucleobase amino acids (NBAs) as protein engineering tools: Construction of NBA-modified HIV-1 protease analogues and enhancement of dimerization induced by nucleobase interaction, Tsuyoshi Takahashi; Dewi Yana; Hisakazu Mihara, May 2006, ChemBioChem, 7, 5, 729, 732, Scientific journal

  DOI
• Binding modes of the precursor of adenovirus major core protein VII to DNA and template activating factor I: Implication for the mechanism of remodeling of the adenovirus chromatin, Béla Gyurcsik; Hirohito Haruki; Tsuyoshi Takahashi; Hisakazu Mihara; Kyosuke Nagata, 10 Jan. 2006, Biochemistry, 45, 1, 303, 313, Scientific journal

  DOI
• Construction and control of self-assembly of amyloid and fibrous peptides, Hisakazu Mihara; Sachiko Matsumura; Tsuyoshi Takahashi, 15 Apr. 2005, Bulletin of the Chemical Society of Japan, 78, 4, 572, 590, Scientific journal

  DOI
• De novo design of peptides with L-α-nucleobase amino acids and their binding properties to the P22 boxB RNA and its mutants, Hideo Miyanishi; Tsuyoshi Takahashi; Hisakazu Mihara, Jul. 2004, Bioconjugate Chemistry, 15, 4, 694, 698, Scientific journal

  DOI
• A peptide-cyclodextrin hybrid system capable of detecting guest molecules utilizing fluorescence resonance energy transfer, Dewi Yana; Tsuyoshi Takahashi; Hisakazu Mihara; Akihiko Ueno, 20 Feb. 2004, Macromolecular Rapid Communications, 25, 4, 577, 581, Scientific journal

  DOI
• Inhibition of peptide amyloid formation by cationic peptides with homologous sequences, Taro Yamashita; Yuta Takahashi; Tsuyoshi Takahashi; Hisakazu Mihara, 17 Nov. 2003, Bioorganic and Medicinal Chemistry Letters, 13, 22, 4051, 4054, Scientific journal

  DOI
• Complementary nucleobase interaction enhances peptide - Peptide recognition and self-replicating catalysis, Sachiko Matsumura; Tsuyoshi Takahashi; Akihiko Ueno; Hisakazu Mihara, 06 Oct. 2003, Chemistry - A European Journal, 9, 19, 4829, 4837, Scientific journal

  DOI
• Nucleobase amino acids incorporated into the HIV-1 nucleocapsid protein increased the binding affinity and specificity for a hairpin RNA, Tsuyoshi Takahashi; Akihiko Ueno; Hisakazu Mihara, 2002, ChemBioChem, 3, 6, 543, 549, Scientific journal

  DOI
• HIV Rev peptides conjugated with peptide nucleic acids and their efficient binding to RRE RNA, Ichiro Kumagai; Tsuyoshi Takahashi; Keita Hamasaki; Akihiko Ueno; Hisakazu Mihara, 07 May 2001, Bioorganic and Medicinal Chemistry Letters, 11, 9, 1169, 1172, Scientific journal

  DOI
• Construction of peptide conjugates with peptide nucleic acids containing an anthracene probe and their interactions with DNA, Ganesan Balasundaram; Tsuyoshi Takahashi; Akihiko Ueno; Hisakazu Mihara, May 2001, Bioorganic and Medicinal Chemistry, 9, 5, 1115, 1121, Scientific journal

  DOI
• Construction of peptides with nucleobase amino acids: Design and synthesis of the nucleobase-conjugated peptides derived from HIV-1 rev and their binding properties to HIV-1 RRE RNA, Tsuyoshi Takahashi; Keita Hamasaki; Akihiko Ueno; Hisakazu Mihara, Apr. 2001, Bioorganic and Medicinal Chemistry, 9, 4, 991, 1000, Scientific journal

  DOI
• Construction of two-stranded α-helix peptides based on influenza virus M1 protein selectively bound to RNA, Hideto Kuribayashi; Tsuyoshi Takahashi; Kyosuke Nagata; Akihiko Ueno; Hisakazu Mihara, 02 Oct. 2000, Bioorganic and Medicinal Chemistry Letters, 10, 19, 2227, 2230, Scientific journal

  DOI
• Design of a nucleobase-conjugated peptide that recognizes HIV-1 RRE IIB RNA with high affinity and specificity, Tsuyoshi Takahashi; Keita Hamasaki; Ichiro Kumagai; Akihiko Ueno; Hisakazu Mihara, 07 Mar. 2000, Chemical Communications, 5, 349, 350, Scientific journal

  DOI
• Construction of HIV Rev peptides containing peptide nucleic acid that bind HIV RRE IIB RNA, Ichiro Kumagai; Tsuyoshi Takahashi; Keita Hamasaki; Akihiko Ueno; Hisakazu Mihara, 21 Feb. 2000, Bioorganic and Medicinal Chemistry Letters, 10, 4, 377, 379, Scientific journal

  DOI
• Development of a β-lactamase activity assay for detecting ligand–protein interactions using an engineered split intein and β-lactamase, Hiroaki Hagiwara; Eriho Fujii; Taisei Iwabuchi; Tsuyoshi Takahashi, 16 Feb. 2024, Bulletin of the Chemical Society of Japan, 97, 3, Scientific journal

  DOI
• 反応性ペプチドタグおよびインテインタグを用いたペプチド-タンパク質間相互作用検出法の開発, 高橋 剛, Aug. 2023, 55, 9, 731

MISC

• Selection of Fluorescein-Binding De Novo Proteins with α3β3 Structures, OKURA Hiromichi; TAKAHASHI Tsuyoshi; MIHARA Hisakazu, 01 Mar. 2011, 2010, 158, 158
• Designed Peptide Libraries for Protein and Cell analyses, KIKUCHI Kazuya; USUI Kenji; TOMIZAKI Kin-ya; TAKAHASHI Tsuyoshi; MIHARA Hisakazu, 01 Mar. 2011, 2010, 21, 21
• モデルペプチドを用いたLEA(Late Embryogenesis Abundant)タンパク質の分子構造とガラス化に与える塩濃縮効果の検討, 古木隆生; 清水天平; 黄川田隆洋; 奥田隆; 高橋剛; 三原久和; 櫻井実, 2010, 46th
• モデルペプチドを用いたLEA(Late Embryogenesis Abundant)タンパク質の分子構造とガラス化の研究, 古木隆生; 清水天平; 黄川田隆洋; 奥田隆; 高橋剛; 三原久和; 櫻井実, 2009, 45th
• 種々の細胞系を用いた新規αヘリックスペプチドの細胞導入活性スクリーニング, 菊池卓哉; 臼井健二; 高橋剛; 三原久和, 2009, 12th
• Design of β-Hairpin Peptides Having Nucleobase Amino Acids (NBAs) and Stabilization of Structures by NBA Interaction, UOZUMI Ryuichi; TAKAHASHI Tsuyoshi; MIHARA Hisakazu, 01 Mar. 2008, 2007, 471, 472
• Construction of Green Fluorescent Protein (GFP) Variants with Amyloid β-Peptide Sequence, TAKAHASHI Tsuyoshi; OHTA Kenichi; MIHARA Hisakazu, 01 Mar. 2007, 2006, 9, 9
• Design of Artificial Proteins and Peptides Targeting to Amyloid β Peptide (Aβ) and Control of Aβ Aggregation, TAKAHASHI Tsuyoshi; MATSUMURA Sachiko; MIHARA Hisakazu, 2007, Seibutsu Butsuri, 47, 4, 228, 234

  DOI
• 生体反応を司るらせん・たんぱく質のらせん構造, 高橋 剛; 三原久和, 2007, 55, 7, 356, 359

  DOI
• In Vitro Selection of RNA Aptamers That Bind to an Oligomeric State of Amyloid β-Peptide, TAKAHASHI Tsuyoshi; TADA Kosuke; MIHARA Hisakazu, 01 Mar. 2006, 2005, 483, 484
• Construction of the Artificial Peptides and RNAs Targeting Amyloid Peptides, TAKAHASHI Tsuyoshi; TADA Kosuke; OSHIMA Hideo; MATSUMURA Sachiko; MIHARA Hisakazu, 01 Mar. 2004, 2003, 455, 456
• De Novo Design of α-Helical Peptides with Nucleobase Amino Acids that Specifically Recognize a Hairpin RNA, MIYANISHI Hideo; TAKAHASHI Tsuyoshi; UENO Akihiko; MIHARA Hisakazu, 01 Feb. 2003, 2002, 369, 370
• Interaction of Basic Peptides Derived from Influenza Virus M1 Protein with DNA/RNA, KURIBAYASHI Hideto; TAKAHASHI Tsuyoshi; UENO Akihiko; MIHARA Hisakazu, 01 Oct. 1998, 1998, 413, 416
• 反応性ペプチドタグおよびインテインタグを用いたペプチド−タンパク質間相互作用検出法の開発, 高橋剛, Aug. 2023, 55, 73, 77

Books etc

• 酵素・タンパク質をはかる・とらえる・利用する（岡畑恵雄, 三原久和編）, 2009
• トコトンやさしいタンパク質の本（東京工業大学大学院生命理工学研究科編）, 2007
• 図解 バイオ活用技術のすべて（東京工業大学大学院生命理工学研究科編）, 2004

Presentations

• Tag Pol-3インテインの改変とスプライシング活性の評価, 大友 真歩、高橋 剛, 日本化学会第105春季年会, 27 Mar. 2025
• ハイマンノース型糖鎖をもつペプチドの合成と糖鎖脱離酵素の活性評価, 井上 遥、相京 碧、高橋 諭、石井 希実、松尾 一郎、吉田 雪子、鈴木 匡、高橋 剛, 日本化学会第105春季年会, 26 Mar. 2025
• 分割インテインと分岐ペプチドを用いた触媒的タンパク質スプライシングの開発, 高橋 剛, 日本化学会第105春季年会, 26 Mar. 2025
• Keap1 円順列変異体の構築と Keap1 結合ペプチドの探索, 信澤 佳奈、高橋 剛, 2024年度日本化学会群馬地区研究交流発表会, 04 Dec. 2024
• 糖鎖を認識するタンパク質 Fbs2 と Nrf1 由来糖ペプチドとの相互作用解析, 相京 碧、井上 遥、高橋 諭、石井 希実、松尾 一郎、吉田 雪子、鈴木 匡、高橋 剛, 2024年度日本化学会群馬地区研究交流発表会, 04 Dec. 2024
• Synthesis of glycopeptides with high-mannose oligosaccharide and investigation of their reactivities toward deglycosylating enzymes, 井上 遥、高橋 諭、石井 希実、松尾 一郎、吉田 雪子、鈴木 匡、高橋 剛, 第61回ペプチド討論会, 29 Oct. 2024, English
• Construction of the selection method to identify proteins that bind to peptide sequences, 清水 もも花、信澤 佳奈、高橋 剛, 第61回ペプチド討論会, 29 Oct. 2024, English
• Construction of circulary permuted Keap1 and selection of peptides that bind to Keap1 using an engineered split intein, 信澤 佳奈、高橋 剛, 第61回ペプチド討論会, 29 Oct. 2024, English
• Analysis of interactions of glycopeptides with sugar-recognizing F-box protein FBS2, 相京 碧、井上 遥、高橋 諭、石井 希実、松尾 一郎、吉田 雪子、鈴木 匡、高橋 剛, 第61回ペプチド討論会, 29 Oct. 2024, English
• ハイマンノース型糖鎖をもつ糖ペプチドの合成と脱糖鎖酵素活性の評価, 井上 遥、高橋 諭、石井 希実、松尾 一郎、吉田 雪子、鈴木 匡、高橋 剛, 第16回東北糖鎖研究会, 05 Oct. 2024
• 糖鎖認識F-boxタンパク質Fbs2とNrf1由来糖ペプチドとの相互作用解析, 相京 碧、井上 遥、高橋 諭、石井 希実、松尾 一郎、吉田 雪子、鈴木 匡、高橋 剛, 第16回東北糖鎖研究会, 05 Oct. 2024
• 分割インテインを用いた標的タンパク質結合ペプチドの探索, 信澤 佳奈、渡辺 雄太郎、高橋 剛, 第18回バイオ関連化学シンポジウム, 12 Sep. 2024, Japanese
• 分割インテインを用いた特定のペプチド配列を認識するタンパク質の探索, 清水 もも花、信澤 佳奈、高橋 剛, 第18回バイオ関連化学シンポジウム, 12 Sep. 2024, Japanese
• 分割インテインとルシフェラーゼを用いた脱糖鎖酵素ENGase活性を発光検出するプローブの開発, 相京 碧、高橋 諭、石井 希実、松尾 一郎、吉田 雪子、鈴木 匡、高橋 剛, 第18回バイオ関連化学シンポジウム, 12 Sep. 2024, Japanese
• 分割インテインを用いたペプチド–タンパク質間相互作用検出法への環状ペプチドの利用, 宇部 早波、信澤 佳奈、高橋 剛, 日本化学会第105春季年会, 26 Mar. 2025
• 分割インテインとカナマイシンキナーゼの再構成を利用した新規リガンド探索法の構築, 信澤 佳奈; 渡辺 雄太郎; 高橋 剛, 日本化学会第103春季年会, 24 Mar. 2023
• 糖ペプチドの合成と細胞質ペプチド:N-グリカナーゼおよびエンド-β-N-アセチルグルコサミニダーゼによる反応性の検討, 井上遥; 高橋諭; 石井希実; 松尾一郎; 吉田雪子; 鈴木匡; 高橋剛, 日本化学会第103春季年会, 24 Mar. 2023
• 分割インテインを用いた細胞質ペプチド:N-グリカナーゼ活性の細胞内発光検出, 高橋剛; 内林達也; 高橋諭; 石井希実; 松尾一郎; 吉田雪子; 鈴木匡, 日本化学会第103春季年会, 24 Mar. 2023
• Selection of peptides capable of binding to MDM2 using an engineered split intein, 渡辺雄太郎; 高橋剛, 第59回ペプチド討論会, 27 Oct. 2022
• Construction of a detection method for peptide: N-glycanase activity using engineered split inteins, 内林達也; 高橋諭; 石井希実; 松尾一郎; 吉田雪子; 鈴木匡; 高橋剛, 第59回ペプチド討論会, 27 Oct. 2022
• 非天然型10糖ペプチドプローブの合成及びPNGase基質特異性解析, 高橋 諭; 石井希実; 内林達也; 佐野加苗; 高橋 剛; 佐藤匡史; 矢木宏和; 加藤晃一; 鈴木 匡; 松尾一郎, 第41回日本糖質学会年会, 29 Sep. 2022
• 分割インテインを用いたペプチド:N-グリカナーゼ活性の発光検出, 高橋剛; 内林達也; 石井希実; 松尾一郎; 吉田雪子; 鈴木匡, 第16回バイオ関連化学シンポジウム, 10 Sep. 2022
• 反応性タグを用いた相互作用検出系による翻訳後修飾ペプチドとSkp1-Fboxタンパク質複合体との相互作用の検出, 巻本亜樹; 高橋 剛, 日本化学会第102春季年会, 24 Mar. 2022
• 分割インテインとカナマイシンキナーゼの再構成を利用したペプチド-タンパク質間相互作用の大腸菌内での検出, 渡辺雄太郎, 高橋 剛, 日本化学会第102春季年会, 24 Mar. 2022
• 分割インテインによるルシフェラーゼ再構成を利用した細胞質ペプチド:N-グリカナーゼ活性検出系の構築, 内林達也; 高橋 諭; 石井希実; 松尾一郎; 吉田雪子; 鈴木 匡; 高橋 剛, 日本化学会第102春季年会, 24 Mar. 2022
• PNGase活性検出に向けた非天然型7糖ペプチドの合成と評価, 高橋 諭; 石井希実; 佐野加苗; 内林達也; 高橋 剛; 佐藤匡史; 矢木宏和; 加藤晃一; 鈴木 匡; 松尾一郎, 日本農芸化学会 2022年度大会, 17 Mar. 2022
• Construction of curculary permutated soybean peroxidase, 岩渕泰世; 高橋 剛, 第58回ペプチド討論会, Oct. 2021
• 合成化合物-タンパク質間相互作用に依存したβラクタマーゼ生成系の構築, 萩原弘顕; 岩渕泰世; 藤井絵里帆; 高橋 剛, 第15回バイオ関連化学シンポジウム, Sep. 2021
• インテインタグを用いた合成化合物－タンパク質間相互作用の検出, 高橋剛, 日本化学会第101春季年会, 19 Mar. 2021, 19 Mar. 2021, 22 Mar. 2021, Japanese, Domestic conference
• 合成化合物－タンパク質間相互作用に依存したβラクタマーゼ生成系の構築, 萩原弘顕、藤井絵里帆、石川佳歩、高橋剛, 日本化学会第101春季年会, 19 Mar. 2021, 19 Mar. 2021, 22 Mar. 2021, Domestic conference
• 分割型大豆由来ペルオキシダーゼと分割型インテイン融合体の構築, 岩渕泰世、高橋剛, 日本化学会第101春季年会, 19 Mar. 2021, 19 Mar. 2021, 22 Mar. 2021, Japanese, Domestic conference
• 分割型大豆由来ペルオキシダーゼと分割型インテイン融合体の構築, 岩渕泰世; 高橋 剛, 日本化学会第101春季年会, Mar. 2021
• インテインタグを用いた合成化合物－タンパク質間相互作用の検出, 高橋剛, 日本化学会第101春季年会, Mar. 2021
• 合成化合物－タンパク質間相互作用に依存したラクタマーゼ生成系の構築, 萩原弘顕; 藤井絵里帆; 石川佳歩; 高橋 剛, 日本化学会第101春季年会, Mar. 2021
• Detection of ligand-protein interactions using reactive peptide and/or intein tags capable of activating reporter enzymes, 高橋剛, 第57回ペプチド討論会, 11 Nov. 2020, 09 Nov. 2020, 11 Nov. 2020, English, Domestic conference
• Detection of ligand-protein interactions using reactive peptide and/or intein tags capable of activating reporter enzymes, 高橋 剛; 萩原弘顕, 第57回ペプチド討論会, Nov. 2020
• 反応性ペプチドタグを用いた合成化合物－タンパク質間相互作用の酵素活性による検出, 高橋剛, 第14回バイオ関連化学シンポジウム, 07 Sep. 2020, 07 Sep. 2020, 08 Sep. 2020, Japanese, Domestic conference
• 反応性ペプチドタグを用いた合成化合物－タンパク質間相互作用の酵素活性による検出, 高橋 剛, 第14回バイオ関連化学シンポジウム, Sep. 2020
• ペプチドタグ間のライゲーションとNanoLucレポーターを用いた合成化合物－タンパク質相互作用の細胞内検出, 高橋 剛; 萩原弘顕, 日本化学会第100春季年会, Mar. 2020
• リガンド－タンパク質間相互作用に依存したタンパク質スプライシングを利 用した活性型酵素生成系の構築, 萩原弘顕; 高橋 剛, 日本化学会第100春季年会, Mar. 2020
• 人工分割インテインの相補的反応を用いたNanoLucルシフェラーゼ生成システムの構築, 高橋 剛, 第13回バイオ関連化学シンポジウム, Sep. 2019
• ペプチドタグの連結とNanoLuc 再構成を利用したアトルバスタチンータンパク質間相互作用の検出, 萩原弘顕; 藤岡芽生子; 高橋 剛, 第13回バイオ関連化学シンポジウム, Sep. 2019
• IDNCL-ER法を用いたがん関連タンパク質に結合するペプチドの探索, 藤岡芽生子; 荒井将吾; 高橋 剛, 第12回バイオ関連化学シンポジウム, Sep. 2018
• NanoLucレポーターを用いたIDNCL-ERの構築とリガンド−タンパク質間相互作用の検出, 高橋 剛, 第12回バイオ関連化学シンポジウム, Sep. 2018
• ペプチド連結反応とルシフェラーゼ再構成を利用したリガンド−タンパク質間相互作用検出系の構築, 高橋 剛, 日本化学会第98春季年会, Mar. 2018
• 酵素活性を指標としたリガンド−タンパク質間相互作用検出法を用いたがん関連タンパク質に結合するペプチドの探索, 藤岡芽生子; 茂木千明; 荒井将吾; 須賀大貴; 高橋 剛, 日本化学会第98春季年会, Mar. 2018
• リガンドータンパク質間の相互作用に依存したネイティブケミカルライゲーション反応における脱離基の影響, 日本化学会第97春季年会, 2017
• Detection and screening of phosphopeptide-SH2 interactions using interaction-dependent native chemical ligation and protein trans-splicing (IDNCL-PTS), 53rd Japanese Peptide Symposium, 2016
• 短鎖ペプチド断片によるタンパク質トランススプライシング反応を用いたシグナル生成系の構築, 第10回バイオ関連化学シンポジウム, 2016
• Detection and screening of phosphopeptide-SH2 interactions using interaction-dependent native chemical ligation and protein trans-splicing (IDNCL-PTS), 53rd Japanese Peptide Symposium, 2016
• Interaction-dependent native chemical ligation and protein slicing for detecting ligand-protein interactions, 高橋 剛; 齋藤彰紀, 第52回ペプチド討論会, Nov. 2015, 16 Nov. 2015, 18 Nov. 2015
• ペプチド連結反応とタンパク質スプライシングを用いたリガンド−タンパク質間相互作用検出系の構築, 高橋 剛; 齋藤彰紀; 茂木千明, 第9回バイオ関連化学シンポジウム, Sep. 2015, 10 Sep. 2015, 12 Sep. 2015
• 分割インテインを用いた標的タンパク質結合ペプチド探索法の開発, 信澤佳奈; 渡辺雄太郎; 高橋剛, 日本ケミカルバイオロジー学会 第18回年会, 28 May 2024, 27 May 2024, 29 May 2024, Japanese
• 分割インテインとルシフェラーゼを用いた脱糖鎖酵素活性の発光検出, 相京碧; 井上遥; 高橋諭; 石井希実; 松尾一郎; 吉田雪子; 木匡; 高橋剛, 日本ケミカルバイオロジー学会 第18回年会, 28 May 2024, 27 May 2024, 29 May 2024, Japanese
• Keap1タンパク質を標的とした分割インテインとカナマイシンキナーゼの再構成によるペプチドの探索, 信澤佳奈; 高橋剛, 20 Mar. 2024, Japanese
• 糖鎖認識F-boxタンパク質Fbs2と糖ペプチドとの相互作用解析, 相京碧; 井上遥; 高橋諭; 石井希実; 松尾一郎; 吉田雪子; 鈴木匡; 高橋剛, 日本化学会第104春季年会, 20 Mar. 2024, Japanese
• 超好熱菌由来システインレスインテインのタンパク質スプライシング活性の評価, 片桐栞太; 高橋剛, 日本化学会第104春季年会, 20 Mar. 2024, Japanese
• 改変型分割インテインを用いた特定のペプチド配列を認識するタンパク質の探索, 清水もも花; 高橋剛, 日本化学会第104春季年会, 20 Mar. 2024, Japanese
• 分割インテインとカナマイシンキナーゼの再構成を利用したKeap1タンパク質に結合するペプチドの探索, 信澤佳奈; 高橋剛, 令和5年度 日本化学会関東支部 群馬地区研究発表交流会, 09 Dec. 2023, Japanese
• 細胞質ペプチド: N-グリカナーゼ活性の細胞内発光検出法の開発, 高橋剛; 石井希実; 松尾一郎; 吉田雪子; 鈴木匡, 第17回東北糖鎖研究会, 11 Nov. 2023, Japanese
• トリマンノシルコア糖鎖をもつ糖ペプチドの合成と脱糖鎖酵素による反応性の検討, 井上遥; 高橋諭; 石井希実; 松尾一郎; 吉田雪子; 鈴木匡; 高橋剛, 第17回東北糖鎖研究会, 11 Nov. 2023, Japanese
• Selection of peptides that bind to Keap1 using intein-based selection method, K. Nobusawa; Y. Watanabe; T. Takahashi, 60th Japanese Peptide Symposium, 08 Nov. 2023, 10 Nov. 2023, English
• Synthesis of glycopeptides and investigation of their reactivities toward deglycosylating enzymes, H. Inoue; S. Takahashi; N. Ishii; I. Matsuo; Y. Yoshida; T. Suzuki; T. Takahashi, 60th Japanese Peptide Symposium, 08 Nov. 2023, 10 Nov. 2023, English
• Development of a selection method to discover peptides that bind to proteins using engineered split intein, T. Takahashi; Y. Watanabe, 60th Japanese Peptide Symposium, 08 Nov. 2023, 10 Nov. 2023, English
• Detection of peptide-Keap1 interaction and selection of Keap1-binding peptides using engineered split intein and kanamycin kinase, K. Nobusawa; Y. Watanabe; T. Takahashi, 13th International Peptide Symposium/15th Australian Peptide Conference, 15 Oct. 2023, 20 Oct. 2023, English
• Design and synthesis of glycopeptides to examine the deglycosylating enzyme activities, H. Inoue; S. Takahashi; N. Ishii; I. Matsuo; Y. Yoshida; T. Suzuki; T. Takahashi, 13th International Peptide Symposium/15th Australian Peptide Conference, 15 Oct. 2023, 20 Oct. 2023, English
• Detection of peptide-MDM2 interactions and selection of MDM2-binding peptides using engineered split intein and kanamycin kinase, Y. Watanabe; T. Takahashi, 13th International Peptide Symposium/15th Australian Peptide Conference, 15 Oct. 2023, 20 Oct. 2023, English
• Detection of cytoplasmic peptide:N-glycanase activity inside cells using split inteins and NanoLuc luciferase, T. Takahashi; N. Ishii; I. Matsuo; Y. Yoshida; T. Suzuki, 13th International Peptide Symposium/15th Australian Peptide Conference, 15 Oct. 2023, 20 Oct. 2023, English

Industrial Property Rights

• Patent right, 特願2019-147849, 09 Aug. 2019, 特開2021-028307, 25 Feb. 2021
• Patent right, 特願2003-304195, 28 Aug. 2003, 特開2005-065660, 17 Mar. 2005, 特許第3992282号, 03 Aug. 2007
• Patent right, 特願2003-295153, 19 Aug. 2003, 特開2005-060336, 10 Mar. 2005, 特許第3910569号, 02 Feb. 2007
• Patent right, 特願2003-304195, 28 Aug. 2003, 特開2005-065660, 17 Mar. 2005
• Patent right, 特願2003-295153, 19 Aug. 2003, 特開2005-060336, 10 Mar. 2005

Awards

• Nov. 2010
• Mar. 2001

Research Projects

• Development of rapid screening method to discover peptides capable of binding to target proteins using split intein, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, 01 Apr. 2022, 31 Mar. 2025, 22K05344

  対象リソースIRI
• Apr. 2023, Mar. 2024, Principal investigator, 0351057-A
• Comprehensive screening of drug-binding proteins using a detection method for their interactions based on chemical reactions between reactive peptide tags, Takahashi Tsuyoshi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, 01 Apr. 2018, 31 Mar. 2022, In the present study, IDNCL-ER, a detection system for ligand-protein interactions, has been improved to discover the proteins that bind to drugs and drug candidates. To this purpose, we employed β-lactamase, which is known as an enzyme to break β-lactam ring, as a reporter enzyme. A split intein, which can self-catalyze protein trans-splicing (PTS) reaction, was also employed to develop the detection method. In the detection method, a synthetic ligand such as atorvastatin, a known cholesterol-lowering drug, was conjugated with the short peptide tag containing the C-terminal sequences of split intein and β-lactamase. When the synthetic ligand binds to a target protein, PDE6D, bearing N-terminal sequences β-lactamase and split intein, PTS reaction occurs and active β-lactamase is generated. Using this method, we have assessed the interactions between atorvastatin and PDE6D in vitro, and in E. coli., 18K05348

  対象リソースIRI
• Construction of novel screening method using enzyme reconstitution and creation of bicyclic functional D-peptides, Takahashi Tsuyoshi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, Apr. 2015, Mar. 2019, In the present study, the novel detection systems (interaction-dependent native chemical ligation and enzyme reconstitution; IDNCL-ER, and protein trans-splicing; IDNCL-PTS) for ligand-protein interactions by reading out the enzyme activities have been constructed. In IDNCL-PTS, beta-galactosidase was employed as a reporter enzyme. In IDNCL-ER, NanoLuc luciferase, which is known as a smaller and brigher luciferase, was employed as a reporter protein. Using these systems, synthetic ligands that bind to Ras, SH2, and Pin1 proteins, related to the cancer, have been screened from the synthetic positional scanning peptide libraries that contain D-amino acids., 15K01818

  対象リソースIRI
• Construction of Detection System for Ligand Screening in Solution Using Peptide Generation based on Molecular Interactions, TAKAHASHI Tsuyoshi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Grant-in-Aid for Challenging Exploratory Research, Gunma University, 01 Apr. 2012, 31 Mar. 2014, In order to develop a screening system in solution for ligands capable of binding to proteins, we have attempted to construct a ligation reaction between peptides bearing a ligand and a protein when the ligand-protein interaction occurs. Moreover, using the generated peptide, enzymatic activity can be generated. Using a model interaction pair, coiled-coil peptides, and ribonuclease S reconstitution, we have successfully demonstrated that the detection system can work well., 24651237

  対象リソースIRI
• Control of amyloidogenic proteins using surface-engineered β-barrel proteins, TAKAHASHI Tsuyoshi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), 2009, 2010, The previous study demonstrated that the engineered β-barrel proteins presenting pseudo-amyloid structures on their surfaces can bind tightly to Alzheimer's amyloid β-peptide (Aβ). In this study, suppression of Aβ toxicity using the engineered β-barrel proteins was evaluated. Moreover, the design strategy using β-barrel proteins was expanded for polyglutamine proteins that relate Huntington's disease., 21710225

  対象リソースIRI
• 2009, Principal investigator
• 2009, Principal investigator
• Construction of fluorescent proteins having amyloid b-peptide (Ab) sequence and FRET detection of Ab aggregates, TAKAHASHI Tsuyoshi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Tokyo Institute of Technology, 2007, 2008, 19710183

  対象リソースIRI
• 2006, 2007, 18031011

  対象リソースIRI
• 2005, 2006, 17651065

  対象リソースIRI
• 2004, 2005, 16041211

  対象リソースIRI
• 2004, 2004, 16011218

  対象リソースIRI
• 2003, 2004, 15750138

  対象リソースIRI
• 2004, Principal investigator
• 2000, 2001, 00J10010

  対象リソースIRI
• Apr. 2024, Mar. 2025, Principal investigator
• 2023, Principal investigator