Department of Materials and Bioscience, Assistant Professor

Koki Kamiya

Gunma University, Graduate School of Science and Technology

Gunma University, Graduate School of Science and Technology

2015, Researcher postdoc lv

Life sciences, Applied biochemistry

Nanotechnology/Materials, Biochemistry

Molecular and Functional Analysis of Pore-Forming Toxin Monalysin From Entomopathogenic Bacterium Pseudomonas entomophila., Nonaka Saori;Salim Emil;Kamiya Koki;Hori Aki;Nainu Firzan;Asri Rangga Meidianto;Masyita Ayu;Nishiuchi Takumi;Takeuchi Shoji;Kodera Noriyuki;Kuraishi Takayuki, 27 Mar. 2020, Frontiers in immunology, 11

Molecular and Functional Analysis of Pore-Forming Toxin Monalysin From Entomopathogenic Bacterium Pseudomonas entomophila, Nonaka, Saori;Salim, Emil;Kamiya, Koki;Hori, Aki;Nainu, Firzan;Asri, Rangga Meidianto;Masyita, Ayu;Nishiuchi, Takumi;Takeuchi, Shoji;Kodera, Noriyuki;Kuraishi, Takayuki, 2020, FRONTIERS IN IMMUNOLOGY, 11

Development of Artificial Cell Models Using Microfluidic Technology and Synthetic Biology, Kamiya, Koki, 2020, MICROMACHINES, 11, 6

A pumpless solution exchange system for nanopore sensors, Yamada, Tetsuya; Kamiya, Koki; Osaki, Toshihisa; Takeuchi, Shoji, Nov. 2019, BIOMICROFLUIDICS, 13, 6, 064104, Scientific journal

Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method, Kamiya Koki; Osaki Toshihisa; Takeuchi Shoji, 25 Sep. 2019, RSC ADVANCES, 9, 52, 30071, 30075

多様な生物学的階層における生命現象理解のための戦略　真核細胞の細胞膜を模倣した人工細胞膜の創成, 神谷 厚輝, Sep. 2019, 92回, [1S15m

Automatic generation system of cell-sized liposomes, Gotanda Masahide; Kamiya Koki; Osaki Toshihisa; Miki Norihisa; Takeuchi Shoji, 01 Aug. 2019, SENSORS AND ACTUATORS B-CHEMICAL, 292, 57, 63

Construction of a Biohybrid Odorant Sensor Using Biological Olfactory Receptors Embedded into Bilayer Lipid Membrane on a Chip., Nobuo Misawa; Satoshi Fujii; Koki Kamiya; Toshihisa Osaki; Tomoyuki Takaku; Yasuhiko Takahashi; Shoji Takeuchi, 22 Mar. 2019, ACS sensors, 4, 3, 711, 716

Automatic generation system of cell-sized liposomes, Gotanda, Masahide;Kamiya, Koki;Osaki, Toshihisa;Miki, Norihisa;Takeuchi, Shoji, 2019, SENSORS AND ACTUATORS B-CHEMICAL, 292, 57, 63

Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method, Kamiya, Koki;Osaki, Toshihisa;Takeuchi, Shoji, 2019, RSC ADVANCES, 9, 52, 30071, 30075

Electrophysiological measurement of ion channels on plasma/organelle membranes using an on-chip lipid bilayer system., Koki Kamiya; Toshihisa Osaki; Kenji Nakao; Ryuji Kawano; Satoshi Fujii; Nobuo Misawa; Masatoshi Hayakawa; Shoji Takeuchi, 30 Nov. 2018, Scientific reports, 8, 1, 17498, 17498

Purification-Free MicroRNA Detection by Using Magnetically Immobilized Nanopores on Liposome Membrane., Satoshi Fujii; Koki Kamiya; Toshihisa Osaki; Nobuo Misawa; Masatoshi Hayakawa; Shoji Takeuchi, 04 Sep. 2018, Analytical chemistry, 90, 17, 10217, 10222

Automatic Planar Asymmetric Lipid Bilayer Membrane Formation toward Biological High-Throughput Assay., Masahide Gotanda; Koki Kamiya; Toshihisa Osaki; Satoshi Fujii; Nobuo Misawa; Norihisa Miki; Shoji Takeuchi, Jul. 2018, Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, 2018, 4496, 4499

Sequential generation of asymmetric lipid vesicles using a pulsed-jetting method in rotational wells, Masahide Gotanda; Koki Kamiya; Toshihisa Osaki; Satoshi Fujii; Nobuo Misawa; Norihisa Miki; Shoji Takeuchi, 15 May 2018, Sensors and Actuators, B: Chemical, 261, 392, 397, Scientific journal

Self-generation of two-dimensional droplet array using oil-water immiscibility and replacement, Yasuga, Hiroki; Kamiya, Koki; Takeuchi, Shoji; Miki, Norihisa, Apr. 2018, LAB ON A CHIP, 18, 7, 1130, 1137, Scientific journal

Well-Controlled Cell-Trapping Systems for Investigating Heterogeneous Cell-Cell Interactions., Koki Kamiya; Yuta Abe; Kosuke Inoue; Toshihisa Osaki; Ryuji Kawano; Norihisa Miki; Shoji Takeuchi, Mar. 2018, Advanced healthcare materials, 7, 6, e1701208

Sidewall electrode-chamber for lipid bilayer formation suitable for rapid access of odors to lipid membrane, Nobuo Misawa; Satoshi Fujii; Koki Kamiya; Toshihisa Osaki; Shoji Takeuchi, 24 Apr. 2018, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2018-, 308, 309, International conference proceedings

Handheld nanopore-based biosensing device, Yusuke Izawa; Toshihisa Osaki; Koki Kamiya; Satoshi Fujii; Nobuo Misawa; Norihisa Miki; Shoji Takeuchi, 24 Apr. 2018, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2018-, 2, 5, International conference proceedings

Nano-sized asymmetric lipid vesicles for drug carrier applications, Koki Kamiya; Toshihisa Osaki; Satoshi Fujii; Nobuo Misawa; Shoji Takeuchi, 24 Apr. 2018, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2018-, 152, 153, International conference proceedings

Breathable fabric meets a lipid bilayer system for rapid vapor detection, Satoshi Fujii; Nobuo Misawa; Koki Kamiya; Toshihisa Osaki; Shoji Takeuchi, 24 Apr. 2018, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2018-, 276, 277, International conference proceedings

Glioblastoma fed by middle meningeal artery and displaying cyst formation soon after repeated implantation of carmustine wafers: A case report., Yamamuro S; Hanashima Y; Yoshimura S; Aoki H; Kamiya K; Takamine Y; Negishi H; Yoshino A, Dec. 2017, Molecular and clinical oncology, 7, 6, 953, 956, Scientific journal

Serial DNA relay in DNA logic gates by electrical fusion and mechanical splitting of droplets, Yasuga, H.; Inoue, K.; Kawano, R.; Takinoue, M.; Osaki, T.; Kamiya, K.; Miki, N.; Takeuchi, S., 2017, PLoS ONE, 12, 7, e0180876, Scientific journal

Pesticide vapor sensing using an aptamer, nanopore, and agarose gel on a chip, Fujii, S.; Nobukawa, A.; Osaki, T.; Morimoto, Y.; Kamiya, K.; Misawa, N.; Takeuchi, S., 2017, Lab on a Chip, 17, 14, 2421, 2425, Scientific journal

Giant liposome formation toward the synthesis of well-defined artificial cells, Kamiya, K.; Takeuchi, S., 2017, Journal of Materials Chemistry B, 5, 30, 5911, 5923, Scientific journal

Towards combinatorial mixing devices without any pumps by open-capillary channels: fundamentals and applications (vol 5, 10263, 2015), Marie Tani; Ryuji Kawano; Koki Kamiya; Ko Okumura, Sep. 2016, SCIENTIFIC REPORTS, 6, 31017

Stability of the Microdroplets for Portable Biosensor, Yusuke Izawa; Toshihisa Osaki; Koki Kamiya; Satoshi Fujii; Norihisa Miki; Shoji Takeuchi, 2016, 2016 38TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC), 2016, 1918, 1921, International conference proceedings

Logic gate operation by DNA translocation through biological nanopores, Yasuga, H.; Kawano, R.; Takinoue, M.; Tsuji, Y.; Osaki, T.; Kamiya, K.; Miki, N.; Takeuchi, S., 2016, PLoS ONE, 11, 2, e0149667, Scientific journal

High-Resolution Vertical Observation of Intracellular Structure Using Magnetically Responsive Microplates, Teshima, T.; Onoe, H.; Tottori, S.; Aonuma, H.; Mizutani, T.; Kamiya, K.; Ishihara, H.; Kanuka, H.; Takeuchi, S., 2016, Small, 12, 25, 3366, 3373, Scientific journal

Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes, Kamiya, K.; Kawano, R.; Osaki, T.; Akiyoshi, K.; Takeuchi, S., 2016, Nature Chemistry, 8, 9, 881, 889, Scientific journal

Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC50) of transporter proteins, Yuta Abe; Koki Kamiya; Toshihisa Osaki; Hirotaka Sasaki; Ryuji Kawano; Norihisa Miki; Shoji Takeuchi, 2015, ANALYST, 140, 16, 5557, 5562, Scientific journal

Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC50) of transporter proteins, Yuta Abe; Koki Kamiya; Toshihisa Osaki; Hirotaka Sasaki; Ryuji Kawano; Norihisa Miki; Shoji Takeuchi, 2015, ANALYST, 140, 16, 5557, 5562, Scientific journal

A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein, Ryuji Kawano; Yutaro Tsuji; Koki Kamiya; Taiga Kodama; Toshihisa Osaki; Norihisa Miki; Shoji Takeuchi, Jul. 2014, PLOS ONE, 9, 7, e102427, Scientific journal

Incorporation of adenylate cyclase into membranes of giant liposomes using membrane fusion with recombinant baculovirus-budded virus particles, Takaaki Mori; Koki Kamiya; Masahiro Tomita; Tetsuro Yoshimura; Kanta Tsumoto, Jun. 2014, BIOTECHNOLOGY LETTERS, 36, 6, 1253, 1261, Scientific journal

Magnetically Responsive Microflaps Reveal Cell Membrane Boundaries from Multiple Angles, Tetsuhiko Teshima; Hiroaki Onoe; Hiroka Aonuma; Kaori Kuribayashi-Shigetomi; Koki Kamiya; Taishi Tonooka; Hirotaka Kanuka; Shoji Takeuchi, May 2014, ADVANCED MATERIALS, 26, 18, 2850, 2856, Scientific journal

Parylene Mobile Microplates Integrated with an Enzymatic Release for Handling of Single Adherent Cells, Tetsuhiko Teshima; Hiroaki Onoe; Kaori Kuribayashi-Shigetomi; Hiroka Aonuma; Koki Kamiya; Hirotaka Ishihara; Hirotaka Kanuka; Shoji Takeuchi, Mar. 2014, SMALL, 10, 5, 912, 921, Scientific journal

Droplet Split-and-Contact Method for High-Throughput Transmembrane Electrical Recording, Yutaro Tsuji; Ryuji Kawano; Toshihisa Osaki; Koki Kamiya; Norihisa Miki; Shoji Takeuchi, Nov. 2013, ANALYTICAL CHEMISTRY, 85, 22, 10913, 10919, Scientific journal

Round-tip dielectrophoresis-based tweezers for single micro-object manipulation, Taiga Kodama; Toshihisa Osaki; Ryuji Kawano; Koki Kamiya; Norihisa Miki; Shoji Takeuchi, Sep. 2013, BIOSENSORS & BIOELECTRONICS, 47, 206, 212, Scientific journal

Automated Parallel Recordings of Topologically Identified Single Ion Channels, Ryuji Kawano; Yutaro Tsuji; Koji Sato; Toshihisa Osaki; Koki Kamiya; Minako Hirano; Toru Ide; Norihisa Miki; Shoji Takeuchi, Jun. 2013, SCIENTIFIC REPORTS, 3, 1995, Scientific journal

Droplet-based lipid bilayer system integrated with microfluidic channels for solution exchange, Yutaro Tsuji; Ryuji Kawano; Toshihisa Osaki; Koki Kamiya; Norihisa Miki; Shoji Takeuchi, 2013, Lab on a Chip, 13, 8, 1476, 1481, Scientific journal

Apoptotic marginal zone deletion of anti-Sm/ribonucleoprotein B cells, Yusuke Kishi; Tetsuya Higuchi; Shirly Phoon; Yasuo Sakamaki; Koki Kamiya; Gabriela Riemekasten; Kazunari Akiyoshi; Martin G. Weigert; Takeshi Tsubata, May 2012, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109, 20, 7811, 7816, Scientific journal

A glass fiber sheet-based electroosmotic lateral flow immunoassay for point-of-care testing, Yuriko Oyama; Toshihisa Osaki; Koki Kamiya; Ryuji Kawano; Tsutomu Honjoh; Haruki Shibata; Toru Ide; Shoji Takeuchi, 2012, LAB ON A CHIP, 12, 24, 5155, 5159, Scientific journal

Single-vesicle estimation of ATP-binding cassette transporters in microfluidic channels, Hirotaka Sasaki; Ryuji Kawano; Toshihisa Osaki; Koki Kamiya; Shoji Takeuchi, 2012, LAB ON A CHIP, 12, 4, 702, 704, Scientific journal

Cadherin-integrated liposomes with potential application in a drug delivery system, Koki Kamiya; Kanta Tsumoto; Tetsuro Yoshimura; Kazunari Akiyoshi, Dec. 2011, BIOMATERIALS, 32, 36, 9899, 9907, Scientific journal

Preparation of Connexin43-Integrated Giant Liposomes by a Baculovirus Expression Liposome Fusion Method, Koki Kamiya; Kanta Tsumoto; Satoko Arakawa; Shigeomi Shimizu; Ikuo Morita; Tetsuro Yoshimura; Kazunari Akiyoshi, Dec. 2010, BIOTECHNOLOGY AND BIOENGINEERING, 107, 5, 836, 843, Scientific journal

Confocal microscopic observation of fusion between baculovirus budded virus envelopes and single giant unilamellar vesicles., Kamiya K; Kobayashi J; Yoshimura T; Tsumoto K, Sep. 2010, Biochimica et biophysica acta, 1798, 9, 1625, 1631

Investigation of Fusion between Nanosized Lipid Vesicles and a Lipid Monolayer Toward Formation of Giant Lipid Vesicles with Various Kinds of Biomolecules, Koki Kamiya; Chika Arisaka; Masato Suzuki, 26 Jan. 2021, Micromachines, 12, 2, 133, 133, Scientific journal

Formation of nano-sized lipid vesicles with asymmetric lipid components using a pulsed-jet flow method, Koki Kamiya; Toshihisa Osaki; Shoji Takeuchi, 15 Jan. 2021, Sensors and Actuators, B: Chemical, 327, Scientific journal

Efficient Lipid Bilayer Formation by Dipping Lipid-Loaded Microperforated Sheet in Aqueous Solution, Nobuo Misawa; Satoshi Fujii; Koki Kamiya; Toshihisa Osaki; Shoji Takeuchi, 05 Jan. 2021, Micromachines, 12, 1, 53, 53, Scientific journal

Highly sensitive VOC detectors using insect olfactory receptors reconstituted into lipid bilayers, Tetsuya Yamada; Hirotaka Sugiura; Hisatoshi Mimura; Koki Kamiya; Toshihisa Osaki; Shoji Takeuchi, Jan. 2021, Science Advances, 7, 3, eabd2013, eabd2013, Scientific journal

A lipid-bilayer-on-a-cup device for pumpless sample exchange, Yoshihisa Ito; Yusuke Izawa; Toshihisa Osaki; Koki Kamiya; Nobuo Misawa; Satoshi Fujii; Hisatoshi Mimura; Norihisa Miki; Shoji Takeuchi, Dec. 2020, Micromachines, 11, 12, 1, 13, Scientific journal

Rapid and Resilient Detection of Toxin Pore Formation Using a Lipid Bilayer Array., Yoshihisa Ito; Toshihisa Osaki; Koki Kamiya; Tetsuya Yamada; Norihisa Miki; Shoji Takeuchi, 16 Nov. 2020, Small (Weinheim an der Bergstrasse, Germany), e2005550, Scientific journal

Development of Artificial Cell Models Using Microfluidic Technology and Synthetic Biology., Koki Kamiya, 30 May 2020, Micromachines, 11, 6, Scientific journal

Molecular and Functional Analysis of Pore-Forming Toxin Monalysin From Entomopathogenic Bacterium Pseudomonas entomophila., Saori Nonaka; Emil Salim; Koki Kamiya; Aki Hori; Firzan Nainu; Rangga Meidianto Asri; Ayu Masyita; Takumi Nishiuchi; Shoji Takeuchi; Noriyuki Kodera; Takayuki Kuraishi, 2020, Frontiers in immunology, 11, 520, 520, Scientific journal

Monolithically fabricated perforated polyimide separator for a planar lipid bilayer devi CE, T. Osaki; M. Kaneko; K. Araki; H. Uehara; T. Ura; H. Hirata; K. Kamiya; S. Fujii; N. Misawa; S. Takeuchi, 24 Apr. 2018, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2018-, 45, 46, International conference proceedings

Suppression of sloshing by utilizing surface energy and geometry in microliter cylindrical well, Y. Izawa; T. Osaki; K. Kamiya; S. Fujii; N. Misawa; S. Takeuchi; N. Miki, 02 Dec. 2017, Sensors and Actuators B: Chemical, 258, 1036, 1041, Scientific journal

ELECTRIC STRESS PRODUCES BILAYER LIPID MEMBRANES BY EXCLUSION OF EXCESSIVE OIL LAYER, Y. Matsunaga; T. Osaki; N. Misawa; S. Fujii; K. Kamiya; N. Miki; S. Takeuchi, 2017, 30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017), 433, 435, International conference proceedings

LIPID BILAYER AT VERTICALLY ALIGNED NANOLITER DROPLETS GENERATED BY TWO-LAYERED MICROFLUIDIC CHANNELS, T. Osaki; T. Kaminski; K. Kamiya; S. Fujii; N. Misawa; P. Garstecki; S. Takeuchi, 2017, 30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017), 1275, 1276, International conference proceedings

MICRORNA DIAGNOSIS USING COMPLEMENTARY DNA THAT BRAKES TRANSIT EVENTS THROUGH A BIOLOGICAL NANOPORE, Toshihisa Osaki; Koki Kamiya; Satoshi Fujii; Shoji Takeuchi, 2016, 2016 IEEE 29TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS), 315, 316, International conference proceedings

NON-SPHERICAL LIPOSOME FORMATION USING 3D-LASER-PRINTED MICRO-CUBE STRUCTURES, K. Inoue; K. Kamiya; T. Osaki; N. Miki; S. Takeuchi, 2016, 2016 IEEE 29TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS), 197, 199, International conference proceedings

Towards combinatorial mixing devices without any pumps by open-capillary channels: fundamentals and applications, Marie Tani; Ryuji Kawano; Koki Kamiya; Ko Okumura, Jun. 2015, SCIENTIFIC REPORTS, 5, Scientific journal

Logarithmic concentration gradients regulated by the width of channels for rapid determination of half maximal inhibitory concentration (IC50) of transporter proteins, Yuta Abe; Hirotaka Sasaki; Koki Kamiya; Toshihisa Osaki; Ryuji Kawano; Norihisa Miki; Shoji Takeuchi, 2015, Analyst, 140, 5557, 5562, Scientific journal

Confocal microscopic observation of fusion between baculovirus budded virus envelopes and single giant unilamellar vesicles., Kamiya K; Kobayashi J; Yoshimura T; Tsumoto K, Sep. 2010, Biochimica et biophysica acta, 1798, 9, 1625, 1631, Scientific journal

Formation of Giant Lipid Vesicle Containing Dual Functions Facilitates Outer Membrane Phospholipase, Seren Ohnishi; Koki Kamiya, 20 Aug. 2021, ACS Synthetic Biology, Scientific journal

Fluid interfacial energy drives the emergence of three-dimensional periodic structures in micropillar scaffolds, Hiroki Yasuga; Emre Iseri; Xi Wei; Kerem Kaya; Giacomo Di Dio; Toshihisa Osaki; Koki Kamiya; Polyxeni Nikolakopoulou; Sebastian Buchmann; Johan Sundin; Shervin Bagheri; Shoji Takeuchi; Anna Herland; Norihisa Miki; Wouter van der Wijngaart, 22 Mar. 2021, Nature Physics, Scientific journal

Three-dimensional liposome assembly toward synthetic tissue, Dong Chel Shin; Yuya Morimoto; Koki Kamiya; Shoji Takeuchi, 2020, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, 993, 994, International conference proceedings

Sequential Formation of Asymmetric Lipid Vesicles for Investigation of Biomolecule Interactions, Gotanda Masahide; Kamiya Koki; Tosihisa Osaki; Fujii Satoshi; Misawa Nobuo; Miki Norihisa; Takeuchi Shoji, 2017, The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec), 2017, 1A1, M08

SEQUENTIAL PRODUCTION OF VARIOUS TYPES OF ASYMMETRIC LIPID VESICLES USING PULSE JET FLOW, Masahide Gotanda; Koki Kamiya; Toshihisa Osaki; Satoshi Fujii; Nobuo Misawa; Norihisa Miki; Shoji Takeuchi, 2017, 2017 19TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS), 572, 574, International conference proceedings

1P320 Cell trapping device for observation of connexin function by a single cell device(28. Bioengineering,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014)), Inoue Kosuke; Kamiya Koki; Abe Yuta; Osaki Toshihisa; Miki Norihisa; Takeuchi Shoji, 2014, Seibutsu Butsuri, 54, 1, S194

2P214 Cell-sized asymmetric lipid vesicles for membrane dynamics observation(13B. Biological & Artificial membrane:Dynamics,Poster), Kamiya Koki; Osaki Toshihisa; Shibasaki Kousuke; Takeuchi Shoji, 2014, Seibutsu Butsuri, 54, 1, S230

3PT143 Size-controlled Giant Liposome Array System with Gentle Hydration(The 50th Annual Meeting of the Biophysical Society of Japan), Osaki Toshihisa; Kamiya Koki; Kuribayashi Kaori; Kawano Ryuji; Takeuchi Shoji, 2012, Seibutsu Butsuri, 52, S165

3PT136 Preparation of cell-sized liposomes by pulsed jet flow(The 50th Annual Meeting of the Biophysical Society of Japan), Kamiya Koki; Kawano Ryuji; Osaki Toshihisa; Takeuchi Syoji, 2012, Seibutsu Butsuri, 52, S164

3PT163 Automated Drug Screening System for Ion Channel Proteins using Artificial Cell Membranes(The 50th Annual Meeting of the Biophysical Society of Japan), Kawano Ryuji; Tsuji Yotaro; Kamiya Koki; Osaki Toshihisa; Hirano Minako; Ide Toru; Miki Norihisa; Takeuchi Shoji, 2012, Seibutsu Butsuri, 52, S169

Modified Outer Membrane Protein-G Nanopores with Expanded and Truncated β-Hairpins for Recognition of Double-Stranded DNA, Toshiyuki Tosaka; Koki Kamiya, 04 Apr. 2022, ACS Applied Nano Materials, Scientific journal

Formation and function of OmpG or OmpA-incorporated liposomes using an in vitro translation system., Koki Kamiya, 11 Feb. 2022, Scientific reports, 12, 1, 2376, 2376, Scientific journal

Control of Enzyme Reaction Initiation inside Giant Unilamellar Vesicles by the Cell-Penetrating Peptide-Mediated Translocation of Cargo Proteins., Akari Miwa; Koki Kamiya, 05 Oct. 2022, ACS synthetic biology, Scientific journal

Odorant Sensor Using Olfactory Receptor Reconstituted in a Lipid Bilayer Membrane with Gas Flow System, Tetsuya Yamada; Hirotaka Sugiura; Hisatoshi Mimura; Koki Kamiya; Toshihisa Osaki; Shoji Takeuchi, Jan. 2020, 2020 IEEE 33rd International Conference on Micro Electro Mechanical Systems (MEMS), International conference proceedings

Artificial cell membrane system for odorant sensor: development of solution exchange driven by superabsorbent polymer for repeatable detection, Tetsuya Yamada; Koki Kamiya; Toshihisa Osaki; Shoji Takeuchi, May 2019, 2019 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN), International conference proceedings

Pumpless Solution Exchange for Repeatable Nanopore Biosensor Driven by Superabsorbent Polymer and Hydrostatic Pressure, Tetsuya Yamada; Koki Kamiya; Toshihisa Osaki; Shoji Takeuchi, Jan. 2019, 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems (MEMS), International conference proceedings

3A1346 On-chip analyses of the function of ATP-binding cassette (ABC) transporters(3A Biol & Artifi memb 4: Transport, Signal transduction,The 49th Annual Meeting of the Biophysical Society of Japan), Sasaki Hirotaka; Kawano Ryuji; Osaki Toshihisa; Kamiya Koki; Takeuchi Shoji, 2011, Seibutsu Butsuri, 51, S106

2P243 Preparation and function of cadhein-integrated liposomes using baculovirus-liposome fusion method(The 48th Annual Meeting of the Biophysical Society of Japan), Kamiya Koki; Tsumoto Kanta; Yoshimura Tetsuro; Akiyoshi Kazunari, 2010, Seibutsu Butsuri, 50, 2, S125

1P-185 Preparation and function of connexin giant proteoliposomes(Biol & Artifi memb.:Structure & Property, The 47th Annual Meeting of the Biophysical Society of Japan), Kamiya Koki; Tsumoto Kanta; Yoshimura Tetsuro; Akiyoshi Kazunari, 2009, Seibutsu Butsuri, 49, S91

2P281 Investigation of pH-induced membrane-fusion properties of baculovirus by microscopic observation of individual giant unilamellar vesicles(40. Membrane structure,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006), Kamiya Koki; Tsumoto Kanta; Yoshimura Tetsuro, 2006, Seibutsu Butsuri, 46, 2, S366

Rapid and Resilient Detection of Toxin Pore Formation Using a Lipid Bilayer Array, Ito, Yoshihisa;Osaki, Toshihisa;Kamiya, Koki;Yamada, Tetsuya;Miki, Norihisa;Takeuchi, Shoji, 2020, SMALL, 16, 49

Formation of nano-sized lipid vesicles with asymmetric lipid components using a pulsed-jet flow method, Kamiya, Koki;Osaki, Toshihisa;Takeuchi, Shoji, 2021, SENSORS AND ACTUATORS B-CHEMICAL, 327

A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange, Ito, Yoshihisa;Izawa, Yusuke;Osaki, Toshihisa;Kamiya, Koki;Misawa, Nobuo;Fujii, Satoshi;Mimura, Hisatoshi;Miki, Norihisa;Takeuchi, Shoji, 2020, MICROMACHINES, 11, 12

Highly sensitive VOC detectors using insect olfactory receptors reconstituted into lipid bilayers, Yamada, Tetsuya;Sugiura, Hirotaka;Mimura, Hisatoshi;Kamiya, Koki;Osaki, Toshihisa;Takeuchi, Shoji, 2021, SCIENCE ADVANCES, 7, 3

Efficient Lipid Bilayer Formation by Dipping Lipid-Loaded Microperforated Sheet in Aqueous Solution, Misawa, Nobuo;Fujii, Satoshi;Kamiya, Koki;Osaki, Toshihisa;Takeuchi, Shoji, 2021, MICROMACHINES, 12, 1

Investigation of Fusion between Nanosized Lipid Vesicles and a Lipid Monolayer Toward Formation of Giant Lipid Vesicles with Various Kinds of Biomolecules, Kamiya, Koki;Arisaka, Chika;Suzuki, Masato, 2021, MICROMACHINES, 12, 2

Control of Outer Membrane Phospholipase a (OmpLA) Functions on an Artificial Lipid Bilayer Membrane, Ohnishi, Seren;Kamiya, Koki, 2021, BIOPHYSICAL JOURNAL, 120, 3, 226A, 226A

Formation of Giant Lipid Vesicle Containing Dual Functions Facilitates Outer Membrane Phospholipase, Ohnishi, Seren;Kamiya, Koki, 2021, ACS SYNTHETIC BIOLOGY, 10, 8, 1837, 1846

Formation and function of OmpG or OmpA-incorporated liposomes using an in vitro translation system, Kamiya, Koki, 2022, SCIENTIFIC REPORTS, 12, 1

Modified Outer Membrane Protein-G Nanopores with Expanded and Truncated beta-Hairpins for Recognition of Double-Stranded DNA, Tosaka, Toshiyuki;Kamiya, Koki, 2022, ACS APPLIED NANO MATERIALS, 5, 5, 6149, 6158

Modified Outer Membrane Protein-G Nanopores with Expanded and Truncated beta-Hairpins for Recognition of Double-Stranded DNA, Tosaka, Toshiyuki;Kamiya, Koki, 2022, ACS APPLIED NANO MATERIALS, 5, 5, 6149, 6158

Control of Enzyme Reaction Initiation inside Giant Unilamellar Vesicles by the Cell-Penetrating Peptide-Mediated Translocation of Cargo Proteins, Miwa, Akari;Kamiya, Koki, 2022, ACS SYNTHETIC BIOLOGY

多様な生物学的階層における生命現象理解のための戦略　真核細胞の細胞膜を模倣した人工細胞膜の創成, 神谷 厚輝, Sep. 2019, 92回, [1S15m

Highly sensitive VOC detectors using insect olfactory receptors reconstituted into lipid bilayers., Yamada Tetsuya;Sugiura Hirotaka;Mimura Hisatoshi;Kamiya Koki;Osaki Toshihisa;Takeuchi Shoji, 13 Jan. 2021, Science advances, 7, 3

Investigation of Fusion between Nanosized Lipid Vesicles and a Lipid Monolayer Toward Formation of Giant Lipid Vesicles with Various Kinds of Biomolecules., Kamiya Koki;Arisaka Chika;Suzuki Masato, 26 Jan. 2021, Micromachines, 12, 2

Formation of Giant Lipid Vesicle Containing Dual Functions Facilitates Outer Membrane Phospholipase., Ohnishi Seren;Kamiya Koki, 14 Jul. 2021, ACS synthetic biology, 10, 8

Formation and function of OmpG or OmpA-incorporated liposomes using an in vitro translation system., Kamiya Koki, 11 Feb. 2022, Scientific reports, 12, 1

Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method., Kamiya Koki;Osaki Toshihisa;Takeuchi Shoji, 24 Sep. 2019, RSC advances, 9, 52

Cell-sized asymmetric phospholipid-amphiphilic protein vesicles with growth, fission, and molecule transportation., Masato Suzuki; Koki Kamiya, 17 Mar. 2023, iScience, 26, 3, 106086, 106086, Scientific journal

Cu(II)-Triggered Ion Channel Properties of a 2,2'-Bipyridine-Modified Amphotericin B., Kosuke Komaki; Soichiro Kasuya; Yusei Toda; Toshiyuki Tosaka; Koki Kamiya; Tomomi Koshiyama, 20 Feb. 2023, ACS applied bio materials, 6, 2, 828, 835, Scientific journal

Molecular Transportation Conversion of Membrane Tension Using a Mechanosensitive Channel in Asymmetric Lipid–Protein Vesicles, Kotaro Baba; Koki Kamiya, 09 Apr. 2024, ACS Applied Materials & Interfaces, Scientific journal

Controlled Self‐Assembly of Vesicles by Electrospray Deposition, Toshihisa Osaki; Koki Kamiya; Ryuji Kawano; Kaori Kuribayashi‐Shigetomi; Shoji Takeuchi, 18 Mar. 2024, Small Structures, Scientific journal

Efficiency of transcription and translation of cell-free protein synthesis systems in cell-sized lipid vesicles with changing lipid composition determined by fluorescence measurements., Akari Miwa; Masatoshi Wakamori; Tetsuro Ariyoshi; Yasushi Okada; Mikako Shirouzu; Takashi Umehara; Koki Kamiya, 03 Feb. 2024, Scientific reports, 14, 1, 2852, 2852, Scientific journal

Formation of Cell-Sized Liposomes Incorporating a β-Barrel-Structured Porin through Rehydration of a Phospholipid-Membrane Protein Dried Film, Toshiyuki Tosaka; Koki Kamiya, 26 Jan. 2024, ACS Omega, Scientific journal

Control of Reversible Formation and Dispersion of the Three Enzyme Networks Integrating DNA Computing., Aoi Mameuda; Masahiro Takinoue; Koki Kamiya, 30 May 2023, Analytical chemistry, 95, 25, 9548, 9554, Scientific journal

Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes., Toshiyuki Tosaka; Koki Kamiya, 13 Apr. 2023, International journal of molecular sciences, 24, 8, Scientific journal

Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes, Tosaka, Toshiyuki;Kamiya, Koki, 2023, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24, 8

Cell penetrating peptides facilitate protein transport into the cell-sized asymmetric lipid vesicles at arbitrary timing, Miwa, Akari;Kamiya, Koki, 2023, BIOPHYSICAL JOURNAL, 122, 3, 368A, 368A

Cell-sized asymmetric phospholipid-amphiphilic protein vesicles with growth, fission, and molecule transportation, Suzuki, Masato;Kamiya, Koki, 2023, ISCIENCE, 26, 3

Control of Reversible Formation and Dispersion of the Three Enzyme Networks Integrating DNA Computing, Mameuda, Aoi;Takinoue, Masahiro;Kamiya, Koki, 2023, ANALYTICAL CHEMISTRY

Control of Enzyme Reaction Initiation inside Giant Unilamellar Vesicles by the Cell-Penetrating Peptide-Mediated Translocation of Cargo Proteins., Miwa Akari;Kamiya Koki, Oct. 2022, ACS synthetic biology, 11, 11

Cu(II)-Triggered Ion Channel Properties of a 2,2'-Bipyridine-Modified Amphotericin B., Komaki Kosuke;Kasuya Soichiro;Toda Yusei;Tosaka Toshiyuki;Kamiya Koki;Koshiyama Tomomi, Jan. 2023, ACS applied bio materials, 6, 2

Cell-sized asymmetric phospholipid-amphiphilic protein vesicles with growth, fission, and molecule transportation., Suzuki Masato;Kamiya Koki, Jan. 2023, iScience, 26, 3

Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes., Tosaka Toshiyuki;Kamiya Koki, Apr. 2023, International journal of molecular sciences, 24, 8

Control of Reversible Formation and Dispersion of the Three Enzyme Networks Integrating DNA Computing., Mameuda Aoi;Takinoue Masahiro;Kamiya Koki, May 2023, Analytical chemistry, 95, 25

Efficiency of transcription and translation of cell-free protein synthesis systems in cell-sized lipid vesicles with changing lipid composition determined by fluorescence measurements., Miwa Akari;Wakamori Masatoshi;Ariyoshi Tetsuro;Okada Yasushi;Shirouzu Mikako;Umehara Takashi;Kamiya Koki, Feb. 2024, Scientific reports, 14, 1

Formation of Cell-Sized Liposomes Incorporating a β-Barrel-Structured Porin through Rehydration of a Phospholipid-Membrane Protein Dried Film., Tosaka Toshiyuki;Kamiya Koki, Jan. 2024, ACS omega, 9, 5

CONNECTABLE DNA-LOGIC OPERATION USING DROPLETS AND RUPTURE/REFORMATION OF BILAYER LIPID MEMBRANES, K. Inoue; R. Kawano; H. Yasuga; M. Takinoue; T. Osaki; K. Kamiya; N. Miki; S. Takeuchi, 2015, 2015 TRANSDUCERS - 2015 18TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS), 739, 742

CONTACTLESS CATCH-AND-RELEASE SYSTEM FOR GIANT LIPOSOMES BASED ON NEGATIVE DIELECTROPHORESIS, Taiga Kodama; Toshihisa Osaki; Ryuji Kawano; Koki Kamiya; Norihisa Miki; Shoji Takeuchi, 2013, 26TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2013), 1169, 1170

SPLIT-AND-CONTACT DEVICE TO FORM PLANAR LIPID BILAYERS, Y. Tsuji; R. Kawano; T. Osaki; K. Kamiya; N. Miki; S. Takeuchi, 2013, 26TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2013), 161, 164

Display of recombinant membrane receptors on giant liposomes: Attempt to construct a cell model with integrated membrane protein systems, Kanta Tsumoto; Koki Kamiya; Tetsuro Yoshimura, 2007, 2007 INTERNATIONAL SYMPOSIUM ON MICRO-NANO MECHATRONICS AND HUMAN SCIENCE, VOLS 1 AND 2, 102, 107

BATCH RELEASE OF MONODISPERSE LIPOSOMES TRIGGERED BY PULSED VOLTAGE STIMULATION, Toshihisa Osaki; Koki Kamiya; Ryuji Kawano; Shoji Takeuchi, 2014, 2014 IEEE 27TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS), 457, 458

RECONSTITUTION AND FUNCTION OF MEMBRANE PROTEINS INTO ASYMMETRIC GIANT LIPOSOMES BY USING A PULSED JET FLOW, Koki Kamiya; Ryuji Kawano; Toshihisa Osaki; Shoji Takeuchi, 2014, 2014 IEEE 27TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS), 288, 289

MECHANICAL CELL PAIRING SYSTEM BY SLIDING PARYLENE RAILS, Yuta Abe; Koki Kamiya; Toshihisa Osaki; Ryuji Kawano; Norihisa Miki; Shoji Takeuchi, 2014, 2014 IEEE 27TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS), 185, 187

【ミクロで拓く分析化学】 液滴接触法による人工細胞膜上でのチャネルタンパク質機能の解析と応用, 神谷 厚輝; 大崎 寿久; 竹内 昌治, Jun. 2015, 分析化学, 64, 6, 441, 449

【生命動態システム科学】 合成生物学 人工細胞 人工細胞モデルの創成, 神谷 厚輝, Oct. 2014, 生体の科学, 65, 5, 492, 493

組換えバキュロウイルス-膜融合により巨大リポソーム膜へ構成したシグナル伝達経路タンパク質とその局在, 大田 亜由; 神谷 厚輝; 舘 昭博; 吉村 哲郎; 冨田 昌弘; 湊元 幹太, Sep. 2011, 日本生化学会大会プログラム・講演要旨集, 84回, 4P, 0364

カドヘリン再構成リポソームの構築と機能, 神谷 厚輝; 湊元 幹太; 吉村 哲郎; 秋吉 一成, May 2011, Drug Delivery System, 26, 3, 290, 290

単一巨大リポソーム観察によるバキュロウイルスエンベロープ融合の可視化解析(Investigation of fusion properties of baculovirus envelopes using microscopic observation on individual giant unilamellar vesicles), 神谷 厚輝; 吉村 哲郎; 湊元 幹太, Nov. 2007, 生物物理, 47, Suppl.1, S179, S179

【生体膜のバイオロジー】新技術 マイクロデバイスを利用した人工脂質二重膜によるタンパク質機能解析, 神谷 厚輝; 竹内 昌治, Jun. 2018, 生体の科学, 69, 3, 252, 257

リン脂質非対称組成の人工細胞膜作製と生体分子相互作用観察, 神谷 厚輝; 竹内 昌治, Apr. 2018, 生化学, 90, 2, 225, 229

Analysis and application of ion channel functions on artificial cell membrane using the droplet contact method, Koki Kamiya; Toshihisa Osaki; Shoji Takeuchi, 07 Jul. 2015, Bunseki Kagaku, 64, 6, 441, 449

Selective lipid-patterning for heterologous giant liposome array, T. Osaki; T. Osaki; K. S. Kuribayashi; R. Kawano; H. Sasaki; K. Kamiya; S. Takeuchi; S. Takeuchi, 01 Dec. 2011, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2, 1137, 1139

Reconstitution of G-Protein Coupled Receptors (GPCRS) into giant liposome array, K. Kamiya; K. Kamiya; T. Osaki; K. Tsumoto; R. Kawano; H. Sasaki; S. Takeuchi; S. Takeuchi, 01 Dec. 2011, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2, 1005, 1007

On-chip single vesicle analyses of ATP-binding cassette (ABC) transporters, H. Sasaki; R. Kawano; T. Osaki; K. Kamiya; S. Takeuchi; S. Takeuchi, 01 Dec. 2011, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 1, 182, 184

Automated drug screening system for ion channel proteins, R. Kawano; Y. Tsuji; Y. Tsuji; M. Hirano; T. Osaki; H. Sasaki; K. Kamiya; N. Miki; N. Miki; T. Ide; T. Ide; S. Takeuchi; S. Takeuchi, 01 Dec. 2011, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 1, 76, 78

A high-throughput drug-screening system targeting ABC-transporters: An application of a microfluidic gradient generator, Y. Abe; Y. Abe; Y. Abe; H. Sasaki; R. Yamamoto; R. Yamamoto; T. Osaki; R. Kawano; K. Kamiya; N. Miki; N. Miki; S. Takeuchi; S. Takeuchi, 01 Dec. 2011, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2, 885, 887

G protein coupled receptors (GPCRs) reconstituted on recombinant proteoliposomes using baculovirus-liposome membrane fusion, Kanta Tsumoto; Koki Kamiya; Sayaka Kitaoka; Shin Ogata; Masahiro Tomita; Tetsuro Yoshimura, 2009, 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science, 202, 207

Reconstitution and microscopic observation of G protein subunits on giant liposomes: Attempt to construct a cell model with functional membrane protein components, Kanta Tsumoto; Yuka Yamazaki; Koki Kamiya; Tetsuro Yoshimura, 2008, 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, 145, 150

Membrane fusion between a giant vesicle and small enveloped particles: Possibilities for the application to construct model cells, Kanta Tsumoto; Koki Kamiya; Tetsuro Yoshimura, 01 Dec. 2006, 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS

pH and temperature sensors mounted into giant lipid vesicles for an environmentally responsive platform, Toshihisa Osaki; Toshihisa Osaki; Koki Kamiya; Koki Kamiya; Shoji Takeuchi; Shoji Takeuchi, 01 Jan. 2014, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 2173, 2174

Direct observation of molecular transport between triplet cells via membrane proteins using a microfluidic system, Kosuke Inoue; Kosuke Inoue; Koki Kamiya; Koki Kamiya; Yuta Abe; Yuta Abe; Toshihisa Osaki; Toshihisa Osaki; Norihisa Miki; Shoji Takeuchi; Shoji Takeuchi, 01 Jan. 2014, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 820, 821

Uniform-sized proteoliposome formation by using electrospray for microscopic membrane protein assays, T. Osaki; T. Osaki; K. Kamiya; R. Kawano; R. Iino; R. Iino; H. Noji; H. Noji; S. Takeuchi; S. Takeuchi, 01 Jan. 2013, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 3, 1698, 1700

Single-stranded DNA detection via chemically modified alamethicin nanopore at single molecule level, R. Kawano; D. Noshiro; T. Osaki; T. Osaki; K. Kamiya; K. Asami; S. Futaki; S. Takeuchi; S. Takeuchi, 01 Jan. 2013, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 1, 32, 34

Micro-scale droplet contact method by mechanical motion: Reproducible and robust lipid bilayer formation, L. N S Zaleha; L. N S Zaleha; R. Kawano; H. Yasuga; H. Yasuga; K. Kamiya; T. Osaki; T. Osaki; N. Miki; N. Miki; S. Takeuchi; S. Takeuchi, 01 Jan. 2013, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 3, 1433, 1435

Mechanical cell contact system by a parylene rail filter for study of cell-cell interaction mediated by connexin gap junction, Y. Abe; Y. Abe; K. Kamiya; T. Osaki; T. Osaki; R. Kawano; K. Akiyoshi; N. Miki; N. Miki; S. Takeuchi; S. Takeuchi, 01 Jan. 2013, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 1, 407, 409

Logic operation in DNA nano device: Electrical input/output through biological nanopore, Kosuke Inoue; Kosuke Inoue; Ryuji Kawano; Hiroki Yasuga; Hiroki Yasuga; Masahiro Takinoue; Toshihisa Osaki; Toshihisa Osaki; Koki Kamiya; Norihisa Miki; Norihisa Miki; Shoji Takeuchi; Shoji Takeuchi, 01 Jan. 2013, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 3, 1881, 1883

Droplet-box: A platform for biological-nanopore-based logical operation using lipid-coated droplet network, H. Yasuga; H. Yasuga; R. Kawano; M. Takinoue; Y. Tsuji; T. Osaki; T. Osaki; K. Kamiya; N. Miki; N. Miki; S. Takeuchi; S. Takeuchi, 01 Jan. 2013, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 3, 1914, 1916

Erratum: Single-vesicle estimation of ATP-binding cassette transporters in microfluidic channels (Lab on a Chip - Miniaturisation for Chemistry and Biology (2012) 12 (702-704) DOI: 10.1039/c2lc21058d), Hirotaka Sasaki; Ryuji Kawano; Toshihisa Osaki; Koki Kamiya; Shoji Takeuchi, 21 Dec. 2012, Lab on a Chip - Miniaturisation for Chemistry and Biology, 12, 5280

Towards artificial cell array system: Encapsulation and hydration technologies integrated in liposome array, Toshihisa Osaki; Koki Kamiya; Ryuji Kawano; Hirotaka Sasaki; Shoji Takeuchi; Shoji Takeuchi, 07 May 2012, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 333, 336

Solution exchange of droplet contacting lipid bilayer system, Yutaro Tsuji; Yutaro Tsuji; Ryuji Kawano; Toshihisa Osaki; Hirotaka Sasaki; Koki Kamiya; Norihisa Miki; Norihisa Miki; Shoji Takeuchi; Shoji Takeuchi, 07 May 2012, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 882, 885

Dierectrophoresis-based tweezers for cell-sized liposome manipulation, Taiga Kodama; Taiga Kodama; Toshihisa Osaki; Ryuji Kawano; Hirotaka Sasaki; Koki Kamiya; Norihisa Miki; Norihisa Miki; Shoji Takeuchi; Shoji Takeuchi, 07 May 2012, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 1029, 1032

Uniform size liposomes on a chip: Observation of transport kinetics through nanopore membrane protein, Toshihisa Osaki; Koki Kamiya; Ryuji Kawano; Shoji Takeuchi; Shoji Takeuchi, 01 Jan. 2012, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 94, 96

Smooth-tip dielectrophoresis based tweesers for single liposome handling, Taiga Kodama; Taiga Kodama; Toshihisa Osaki; Ryuji Kawano; Koki Kamiya; Norihisa Miki; Norihisa Miki; Shoji Takeuchi; Shoji Takeuchi, 01 Jan. 2012, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 1081, 1083

Rapid perfusion system for inhibition investigation of membrane proteins in planar lipid bilayer, Y. Tsuji; Y. Tsuji; R. Kawano; T. Osaki; K. Kamiya; N. Miki; N. Miki; S. Takeuchi; S. Takeuchi, 01 Jan. 2012, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 683, 685

Rapid and accurate IC50 determination using logarithmic concentration generator, Yuta Abe; Yuta Abe; Hirotaka Sasaki; Toshihisa Osaki; Koki Kamiya; Ryuji Kawano; Norihisa Miki; Norihisa Miki; Shoji Takeuchi, 01 Jan. 2012, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 956, 958

Portable membrane protein chip: Development of membrane protein sensors for environment analysis, Ryuji Kawano; Yutaro Tsuji; Yutaro Tsuji; Toshihisa Osaki; Koki Kamiya; Norihisa Miki; Norihisa Miki; Yoke Tanaka; Shoji Takeuchi; Shoji Takeuchi, 01 Jan. 2012, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 845, 847

Glass fiber sheet on a chip: For rapid, low-cost, and contamination-free quantitative immunoassay, Yuriko Oyama; Yuriko Oyama; Toshihisa Osaki; Koki Kamiya; Ryuji Kawano; Tsutomu Honjoh; Haruki Shibata; Toru Ide; Shoji Takeuchi, 01 Jan. 2012, Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 1957, 1959

Synthetic nanocage formed by rhodium-organic cuboctahedra: For single molecule detection in lipid bilayer, R. Kawano; S. Furukawa; D. Kiriya; T. Osaki; K. Kamiya; S. Kitagawa; S. Takeuchi, 2013, 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, 854, 855

Droplet network connected by biological nanopores for DNA computing, H. Yasuga; R. Kawano; M. Takinoue; Y. Tsuji; T. Osaki; K. Kamiya; N. Miki; S. Takeuchi, 2013, 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, 1221, 1222

Confocal laser scanning microscopic observation of deformation, biological reaction, and contact of cells using mechanical trapping system with parylene micro filter, Y. Abe; K. Kamiya; T. Osaki; R. Kawano; N. Miki; S. Takeuchi, 2013, 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, 416, 417

Vesicles in a vesicle: Formation of a cell-sized vesicle containing small vesicles from two planar lipid bilayers using pulsed jet flow, Koki Kamiya; Ryuji Kawano; Toshihisa Osaki; Shoji Takeuchi; Shoji Takeuchi, 02 Apr. 2013, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 935, 936

Pendant liposome system to access the internal solution, Toshihisa Osaki; Koki Kamiya; Ryuji Kawano; Shoji Takeuchi, 2013, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 359, 360

Mechanical pumpless giant liposome trapping system using parylene micro filter for biological assay, Y. Abe; K. Kamiya; T. Osaki; R. Kawano; N. Miki; S. Takeuchi, 2013, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 949, 950

Logic gate using artificial cell-membrane: NAND operation by transmembrane DNA via a biological nanopore, Hiroki Yasuga; Hiroki Yasuga; Ryuji Kawano; Masahiro Takinoue; Yutaro Tsuji; Yutaro Tsuji; Toshihisa Osaki; Koki Kamiya; Norihisa Miki; Norihisa Miki; Shoji Takeuchi; Shoji Takeuchi, 02 Apr. 2013, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 1005, 1006

Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level, R. Kawano; Y. Tsuji; Y. Tsuji; M. Hirano; M. Hirano; T. Osaki; K. Kamiya; N. Miki; N. Miki; T. Ide; T. Ide; S. Takeuchi; S. Takeuchi, 02 Apr. 2013, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 57, 58

人工細胞作製に向けたリン脂質非対称膜リポソームの作製, 神谷厚輝; 大崎寿久; 竹内昌治, 2014, 日本分子生物学会年会プログラム・要旨集(Web), 37th, 1P-0423 (WEB ONLY)

リン脂質非対称膜リポソームによる生体分子の挙動観察, 神谷厚輝; 神谷厚輝; 大崎寿久; 大崎寿久; 川野竜司; 竹内昌治; 竹内昌治, 15 Jul. 2015, バイオ・高分子シンポジウム講演要旨集, 25th, 17, 18

リン脂質非対称リポソームによるペプチド相互作用の観察, 神谷厚輝; 大崎寿久; 川野竜司; 竹内昌治, 26 May 2015, 日本蛋白質科学会年会プログラム・要旨集, 15th, 85

4.人工細胞膜作製とシングルイオンチャネル計測, 神谷厚輝; 大崎寿久; 竹内昌治, 2015, 電気化学および工業物理化学, 83, 12, 1096-1100 (J-STAGE)

Cell trapping system for the observation of cell-cell interaction, 井上晃佑; 神谷厚輝; 阿部裕太; 大崎寿久; 三木則尚; 竹中昌治; 竹中昌治, 02 Dec. 2014, 電気学会バイオ・マイクロシステム研究会資料, BMS-14, 28-37, 23, 26

生命動態システム科学 III.合成生物学 1.人工細胞(1)人工細胞モデルの創成, 神谷厚輝, 15 Oct. 2014, 生体の科学, 65, 5, 492, 493

微細加工表面への浸透現象 V, 谷茉莉; 川野竜司; 神谷厚輝; 奥村剛, 22 Aug. 2014, 日本物理学会講演概要集, 69, 2, 225

リン脂質非対称膜リポソームへの膜タンパク質の再構成, 神谷厚輝; 大崎寿久; 川野竜司; 竹内昌治, 12 Mar. 2014, 日本化学会講演予稿集, 94th, 3, 894

MEMS技術を基盤とした生体ナノポアセンシング, 川野竜司; 神谷厚輝; 大崎寿久; 竹内昌治, 12 Mar. 2014, 日本化学会講演予稿集, 94th, 2, 324

1P1-V05 Application to the Drug-Screening System Using Microfluidic Gradient Generator(Nano/Micro Fluid System), Abe Yuta; Sasaki Hirotaka; Osaki Toshihisa; Kawano Ryuji; Kamiya Koki; Miki Norihisa; Takeuchi Shoji, 27 May 2012, ロボティクス・メカトロニクス講演会講演概要集, 2012, "1P1, V05(1)"-"1P1-V05(2)"

4．人工細胞膜作製とシングルイオンチャネル計測, 神谷 厚輝; 大崎 寿久; 竹内 昌治, 2015, 83, 12, 1096, 1100

2P267 Investigation of fusion properties of baculovirus envelopes using microscopic observation on individual giant unilamellar vesicles(Native and artificial biomembranes-structure and properties,Poster Presentations), Kamiya Koki; Yoshimura Tetsuro; Tsumoto Kanta, 20 Nov. 2007, Biophysics, 47, 1, S179

The investigation of membrane protein property by cell trapping system, 井上 晃佑; 神谷 厚輝; 阿部 裕太, 20 Oct. 2014, 「センサ・マイクロマシンと応用システム」シンポジウム論文集 電気学会センサ・マイクロマシン部門 [編], 31, 1, 4

10aAM-7 Imbibition of Patterned Surfaces V, Tani Marie; Kawano Ryuji; Kamiya Koki; Okumura Ko, 22 Aug. 2014, Meeting abstracts of the Physical Society of Japan, 69, 2, 225, 225

3P2-F07 Construction and connecting logic gate using channel membrane proteins and DNA(Nano/Micro Fluid System), INOUE Kosuke; KAWANO Ryuji; YASUGA Hiroki; TAKINOUE Masahiro; KAMIYA Koki; OSAKI Toshihisa; MIKI Norihisa; TAKEUCHI Shoji, 24 May 2014, ロボティクス・メカトロニクス講演会講演概要集, 2014, 3P2, F07(1)-"3P2-F07(2)"

7AM2-C-7 DNA logic calculation using the membrane protein reconstituted artificial cell membrane, INOUE Kosuke; KAWANO Ryuji; YASUGA Hiroki; TAKINOUE Masahiro; KAMIYA Koki; OSAKI Toshihisa; MIKI Norihisa; TAKEUCHI Shoji, 04 Nov. 2013, マイクロ・ナノ工学シンポジウム, 2013, 5, 251, 252

6PM3-PMN-036 MICRO-SCALE DROPLET CONTACT METHOD BY MECHANICAL MOTION : REPRODUCIBLE AND ROBUST LIPID BILAYER FORMATION, ZALEHA L. N. S; Kawano R; Yasuga H; Kamiya K; Osaki T; Miki N; Takeuchi S, 04 Nov. 2013, マイクロ・ナノ工学シンポジウム, 2013, 5, 211, 212

5PM1-C-4 Function Analysis of Membrane Protein by Mechanical Cell and Liposome Trapping System, Abe Yuta; Kamiya Koki; Osaki Toshihisa; Kawano Ryuji; Miki Norihisa; Takeuchi Shoji, 04 Nov. 2013, マイクロ・ナノ工学シンポジウム, 2013, 5, 31, 32

Development of Membrane Protein Chip for the High-throughput Screenings based on MEMS Technology, KAWANO Ryuji; OSAKI Toshihisa; KAMIYA Koki; TAKEUCHI Shoji, 01 Oct. 2013, 自動車技術, 67, 10, 98, 99

1A2-O02 The Formation of DNA Logic Gate on Lipid covered droplet Network(Nano/Micro Fluid System), YASUGA Hiroki; KAWANO Ryuji; TAKINOUE Masahiro; TSUJI Yutaro; OSAKI Toshihisa; KAMIYA Koki; MIKI Norihisa; TAKEUCHI Shoji, 22 May 2013, ロボティクス・メカトロニクス講演会講演概要集, 2013, 1A2, O02(1)"-"1A2-O02(3)"

Single-vesicle estimation of ATP-binding cassette transporters in microfluidic channels (vol 12, pg 702, 2012), Hirotaka Sasaki; Ryuji Kawano; Toshihisa Osaki; Koki Kamiya; Shoji Takeuchi, 2012, LAB ON A CHIP, 12, 24, 5280, 5280, Others

Microfluidic Analysis of ATP-Binding Cassette Transporters at Single-Vesicle Level, Hirotaka Sasaki; Ryuji Kawano; Toshihisa Osaki; Koki Kamiya; Shoji Takeuchi, Jan. 2012, BIOPHYSICAL JOURNAL, 102, 3, 659A, 659A, Summary international conference

TMC6: A Novel Salt Taste Receptor in Mice, Takami Maekawa; Koki Kamiya; Tooru Takahashi; Kentaro Kaji; Yukio Tezuka; Toshihisa Osaki; Shoji Takeuchi; Takashi Kondoh, Sep. 2019, CHEMICAL SENSES, 44, 7, E114, E114, Summary international conference

3P2-H08 Effect of Droplets Interface Area on Mechanical Properties of Artificial Bilayer Lipid Membranes Formed by Droplet Contact Method(Nano/Micro Fluid System), ZALEHA Lylatul Nasihah binti Salleh; KAWANO Ryuji; OSAKI Toshihisa; KAMIYA Koki; MIKI Norihisa; TAKEUCHI Shouji, 24 May 2014, 2014, "3P2, H08(1)"-"3P2-H08(4)"

Molecular and Functional characterization of Monalysin, a pore-forming toxin from Drosophila pathogen Pseudomonas entomophila, EMIL Salim; EMIL Salim; 野中さおり; 神谷厚輝; 堀亜紀; 西内巧; 竹内昌治; 古寺哲幸; 倉石貴透, 2020, 日本分子生物学会年会プログラム・要旨集(Web), 43rd

Detection of microRNA in crude sample, 藤井 聡志; 神谷 厚輝; 大崎 寿久; 三澤 宣雄; 竹内 昌治, Oct. 2017, 16, 2, 46, 49

デジタルバイオ分析(5)人工細胞膜を利用した1分子検出, 藤井 聡志; 神谷 厚輝; 竹内 昌治, Aug. 2016, 545, 53, 57

29pm2-E-4 Formation of droplet network connected with artificial lipid bilayers using droplet-box, YASUGA Hiroki; KAWANO Ryuji; TAKINOUE Masahiro; TSUJI Yutaro; OSAKI Toshihisa; KAMIYA Koki; MIKI Norihisa; TAKEUCHI Shoji, 21 Oct. 2015, 2015, 7, "29pm2, E-4-1"-"29pm2-E-4-2"

29pm3-PN-41 Suppression of resonance of microdroplets, IZAWA Yusuke; OSAKI Toshihisa; KAMIYA Koki; MIKI Norihisa; TAKEUCHI Shoji, 21 Oct. 2015, 2015, 7, "29pm3, PN-41-1"-"29pm3-PN-41-2"

人工細胞膜を用いたイオンチャネル創薬スクリーニングシステム, 川野竜司; 辻祐太郎; 辻祐太郎; 神谷厚輝; 大崎寿久; 平野美奈子; 平野美奈子; 井出徹; 井出徹; 三木典尚; 三木典尚; 竹内昌治; 竹内昌治, 2012, 61, 2

Synthetic Biology, Toshihisa Osaki, Koki Kamiya, and Shoji Takeuchi, Dec. 2014

人工細胞の創製とその応用, Jan. 2017

真核細胞の細胞膜を模倣した人工細胞膜の創成, 神谷厚輝, 第92回日本生化学会大会, 18 Sep. 2019

Asymmetric lipid vesicle generation for creating complex artificial cells, Koki Kamiya, MARSS2019, 02 Jul. 2019

マイクロデバイスによる細胞膜模倣型人工細胞膜の創成と機能観察, 神谷厚輝, 化学とマイクロ・ナノシステム学会 第39回研究会, 28 May 2019

細胞膜環境を模倣した人工細胞膜の創出, 神谷厚輝, 日本化学会　第99春季年会, 18 Mar. 2019

Preparation of artificial cell models by bottom-up approach, Koki Kamiya, 第53回生物物理学会年会, 15 Sep. 2015

FORMATION OF CELL-SIZED ASYMMETRIC LIPID VESICLES WITH LIPID MICRODOMAINS, Koki Kamiya, Toshihisa Osaki, Shoji Takeuchi, MicroTAS2018, 14 Nov. 2018

Crude Planar Cell Membrane On A Chip, Koki Kamiya, Toshihisa Osaki, Kenji Nakao, Nobuo Misawa, Satoshi Fujii, and Shoji Takeuchi, MicroTAS 2016, 10 Oct. 2016

Observation of lipid motion in asymmetric giant vesicles, Koki Kamiya, Toshihisa Osaki and Shoji Takeuchi, MicroTAS 2015, 28 Oct. 2015

Single-channel current measurement of a connexin hemichannel expressed using an in vitro protein synthesis system, Koki Kamiya, Toshihisa Osaki, Ryuji Kawano, Minato Akiyama, Kazunari Akiyoshi and Shoji Takeuchi, MicroTAS 2014, 27 Oct. 2014

Reconstitution and function of membrane proteins into asymmetric giant liposomes by using a pulsed jet flow, Koki Kamiya, Ryuji Kawano, Toshihisa Osaki, and Shoji Takeuchi, IEEE MEMS2014, 29 Jan. 2014

Protein expression inside oil-free giant vesicles by using pulsed jet flow method, Koki Kamiya, R. Kawano, T. Osaki and S. Takeuchi,, MicroTAS 2013, 28 Oct. 2013

Vesicles in a vesicle: formation of a cell-sized vesicle containing small vesicles from two planar lipid bilayers using pulsed jet flow, Koki Kamiya, Ryuji Kawano, Toshihisa Osaki, Shoji Takeuchi, IEEE MEMS 2013, 24 Jan. 2013

Reconstitution of G-Protein Coupled Receptors (GPCRs) into Giant Liposome Array, Koki Kamiya, Toshihisa Osaki，Kanta Tsumoto，Ryuji Kawano, Hirotaka Sasaki and Shoji Takeuchi, MicroTAS 2011, 03 Oct. 2011

Preparation of connexin liposomes using baculovirus expression-liposome fusion systems, Koki Kamiya, Kanta Tsumoto, Tetsuro Yoshimura, and Kazunari Akiyoshi, International liposome research days and Lipids, Liposomes & Membrane Biophysics , 05 Aug. 2010

New method of preparation of proteoliposomes: Baculovirus expression-liposome fusion systems, Koki Kamiya, Kanta Tsumoto, Tetsuro Yoshimura, and Kazunari Akiyoshi., 2nd Switzerland- Japan Biomolecular Chemistry Symposium, 25 Sep. 2009

Preparation of connexin proteoliposomes using baculovirus expression systems for potential biomedical application, Koki Kamiya, Kanta Tsumoto, Tetsuro Yoshimura, and Kazunari Akiyoshi, 36th Annual Meeting & Exposition of the Controlled Release Society , 15 Jul. 2009

Preparation of connexin proteoliposomes using baculovirus expression systems for a novel drug carrier, Koki Kamiya, Kanta Tsumoto, Tetsuro Yoshimura, and Kazunari Akiyoshi, 8th International Symposium on Frontiers in Biomedical Polymers, 15 May 2009

Investigation of pH-induced membrane-fusion properties of baculovirus by microscopic observation of individual giant unilamellar vesicles, Koki Kamiya, Kanta Tsumoto, and Tetsuro Yoshimura, Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan , 10 Nov. 2006

小胞輸送を模倣したリン脂質非対称膜の融合観察, 神谷厚輝、大崎寿久、竹内昌治, 化学とマイクロ・ナノシステム学会第38 回研究会, 01 Nov. 2018

Formation of giant vesicle containing small vesicles with asymmetric lipid membranes, Koki Kamiya, Toshihisa Osaki, Shoji Takeuchi, 第５６回日本生物物理学会年会, 15 Sep. 2018

Artificial cell assembly using bottom-up approach, Koki Kamiya, 第23回京都大学iCeMS国際シンポジウム Emerging Science for Unlocking Cell’s Secrets　, 30 May 2017

膜タンパク質やリン脂質非対称膜を再構成した人工細胞膜の作製, 神谷厚輝、竹内昌治, 細胞を創る研究会8.0, 12 Nov. 2015

人工細胞膜によるイオンチャネル計測とその応用, 神谷厚輝, Nanion・東京女子医大イオンチャネルフォーラム2018, 12 Oct. 2018

NANO-SIZED ASYMMETRIC LIPID VESICLES FOR DRUG CARRIER APPLICATIONS, Koki Kamiya, Toshihisa Osaki, Satoshi Fujii, Nobuo Misawa,and Shoji Takeuchi, IEEE MEMS 2018, 24 Jan. 2018

FORMATION OF A GIANT LIPID VESICLE CONTAINING TWO TYPES OF LIQUID SOLUTIONS USING A THETA-GLASS CAPILLARY, Koki Kamiya, Toshihisa Osaki, Satoshi Fujii, Nobuo Misawa, and Shoji Takeuchi, MicroTAS 2017, 24 Oct. 2017

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May 2019

Mar. 2019

Mar. 2013

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30 Jul. 2020, 31 Mar. 2022, 20K21406

01 Apr. 2020, 31 Mar. 2022, 20H04692

Investigation of membrane protein functions by changing curvature and asymmetric lipid membrane of vesicles, Koki Kamiya, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Gunma University, 01 Apr. 2018, 31 Mar. 2021, Cell membranes of eukaryotic cells were composed of an asymmetric lipid bilayer. In this study, to understand biological phenomena involved in the asymmetric lipid bilayer, we generated an automatic device of cell-sized asymmetric lipid vesicles, nano-sized asymmetric lipid vesicles, vesicles-in-a-vesicle with asymmetric lipid membranes, and cell-sized asymmetric lipid vesicles using a fusion between nano-sized lipid vesicles and a planar lipid bilayer., 18H02092

Investigation of biomolecular activity in liposomes mimicking the surface of cell membranes, Kamiya Koki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory), Grant-in-Aid for Challenging Research (Exploratory), Gunma University, 29 Jun. 2018, 31 Mar. 2020, Giant lipid vesicles with asymmetric lipid bilayer which mimics a plasma membrane of eukaryotic cells were generated by applying a jet flow against a planar asymmetric lipid bilayer (pulsed jet flow method). The plasma membranes are also composed of microdomains and glycolipids. In this study, to improve emulations of the plasma membrane of eukaryotic cells, cell-sized asymmetric lipid vesicles with microdomains and glycolipids were generated by the pulsed jet flow method., 18K19157

2018, 2020, 17KK0107

Creation of non-spherical liposomes using a microdevice, Kamiya Koki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Grant-in-Aid for Challenging Exploratory Research, 01 Apr. 2016, 31 Mar. 2018, I developed a cell-sized non-spherical liposome formation using three-dimensional micro structures to emulate a surface structure of cultured cells. I fabricated a micro-cubic frame based on a stackable photoresist processed using two photon laser writing. Next, I formed lipid membranes on the faces of the micro-cubic frame structures into microfluidic devices. Moreover, to confirm the formation of the lipid bilayer membranes on the faces of the micro-cubic frame structures, nanopore-forming membrane proteins were reconstituted into the non-spherical liposomes. I observed the diffusion of fluorescent molecules through the nanopore-forming membrane proteins., 16K13656

Analysis of salicylic acid response in a single plant cell using microfluidics, BETSUYAKU Shigeyuki; TAKINOUE Masahiro; KAMIYA Koki; TABATA Kazuhito, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Grant-in-Aid for Challenging Exploratory Research, University of Tsukuba, 01 Apr. 2016, 31 Mar. 2018, To understand plant cellular response against salicylic acid (SA), a hormone required for plant immune response, we aimed to establish a system to analyze the SA response at the single cell level using microfluidic devices and the protoplast derived from Arabidopsis transgenic plants carrying YFP-NLS under the control of the SA-responsive PR1 promoter (pPR1 plants). While we established the 2nd generation pPR1 plants that should enable us to perform genetic analysis as well as highly sensitive imaging, we also conditioned microfluidics-based single cell analysis using the 1st generation pPR1 plants with YFP-NLS as a reporter. In this study, the protoplast from the 1st generation pPR1 plants did not show any YFP signal in response to SA treatment, although the leaf cells of the same pPR1 plant exhibited YFP signal clearly after SA treatment. We are currently investigating the reason of this unresponsiveness only found in protoplasts., 16K14750

Interaction of membrane proteins on asymmetric lipid membrane toward creation of artificial translocon, Kamiya Koki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A), Grant-in-Aid for Young Scientists (A), 01 Apr. 2015, 31 Mar. 2018, In this study, I investigated interactions between asymmetric lipid membranes and biological molecules (peptides or membrane proteins). The asymmetric lipid vesicles were generated from a planar lipid bilayer by applying the pulsed jet flow. I achieved three items. Frist, I found that the reconstruction amounts of the membrane proteins into the lipid membranes changed by the lipid compositions, Second, I developed a device for sequentially generating various types of asymmetric lipid vesicles. Third, I developed a well-controlled cell or liposome-trapping device., 15H05493

Development of High-throughput Platform for Ion Channel Analysis Using Artificial Cell Membrane Systems, Takeuchi Shoji; SATO Koji; IDE Toru, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Grant-in-Aid for Scientific Research (A), Kanagawa Academy of Science and Technology, 01 Apr. 2013, 31 Mar. 2017, In this study, we developed a high-throughput platform for analyses of ion channel functions. A cell membrane model was artificially reconstructed on a microchip, in which we optimized the efficiency and reproducibility of the membrane formation and integrated the number of the membrane on a single chip. Accordingly, we examined reconstitution techniques of ion channels into the membrane on the chip, and finally verified the developed platform using functional assays of drug-target ion channels. The results demonstrated the feasibility of our platform, especially for precise ion channel analyses with a single-molecule level. Based on the developed technologies, we continuously improve the performance of the platform, hoping for practical use in drug screening of ion channels., 25246017

Preparation and function of the membrane protein-reconstituted asymmetric liposomes, KAMIYA Koki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Grant-in-Aid for Challenging Exploratory Research, Kanagawa Academy of Science and Technology, 01 Apr. 2013, 31 Mar. 2015, In this study, we investigated the flop dynamics of lipid asymmetric GV membranes in the presence of a membrane-interacted peptide. Our lipid asymmetric GVs were generated by a pulsed jet flow applied to a planar lipid bilayer. We observed that the DOPS-flop in asymmetric GV membranes was promoted in the presence of the peptides. Next, we successfully reconstituted flippases into the asymmetric GVs of phosphatidylserine. Finally, we observed the translocation of lipids from the extracellular leaflet to the cytoplasmic leaflet which was catalyzed by flippases. The asymmetric GVs will be useful for the study of elementary lipid-lipid or lipid-protein interactions., 25620138

Formation of uniformed giant liposome using microplates, SHIGETOMI Kaori; HIROAKI Onoe; KOKI Kamiya, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 01 Apr. 2012, 31 Mar. 2014, In this research, we developed a microdevice that can produce uniformed sized giant liposome efficiently, and can be handled. The permalloy was patterned on the microplate of parylene using photolithographic technique and coated with the parylene after that. The produced microplate could be easily moved using the neodymium magnet. The pattern of the phosphatide lipids were produced on the produced microplate, and then giant liposomes were formed by the hydrating method., 24656161

Construction of artificial synapse liposome array using the vesicular transport liposome model, KAMIYA Koki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up, Grant-in-Aid for Research Activity Start-up, Kanagawa Academy of Science and Technology, 2011, 2012, This study conducted the preparation of asymmetric cell-sized lipid vesicles from a planar lipid bilayer using pulsed jet flow. First, cell-sized vesicles (diameter of 5 μm in over 1 day) were stably formed using an improved double-well device wherein a separator with a 150-μm-hole diameter, was mounted between the wells. Moreover, asymmetric vesicles of phosphatidylserine lipids were successfully formed on each leaflet using this device. The asymmetric cell-sized lipid vesicles will create new opportunities for artificial cell research model such as the interactions between lipid membranes and proteins., 23850022

2008, 2010, 08J04817

09 Jul. 2021, 31 Mar. 2023, 21K19039

01 Apr. 2022, 31 Mar. 2025, 22H01874

07 Oct. 2021, 31 Mar. 2024, 21KK0174

Oct. 2021, Mar. 2024, Principal investigator

Apr. 2021, Mar. 2023, Principal investigator

Oct. 2021, Sep. 2022, Principal investigator

2020, 2022, Principal investigator

2019, 2020, Principal investigator

2020, Principal investigator

2013, 2016, 13415458

01 Apr. 2023, 31 Mar. 2025, 23H04398

01 Apr. 2024, 31 Mar. 2027, 24H00889

30 Jun. 2023, 31 Mar. 2025, 23K17968