Researcher Profile and Settings

Researcher

• Name

  IWASAKI Hirohide

Profile and Settings

• Name

  Iwasaki, Hirohide

Website

• URI

  https://connectome.med.gunma-u.ac.jp/

Affiliation

• Gunma University, Graduate School of Medicine, Anatomy

Research Experience

• Dec. 2018, 9999, Gunma University, Department of Anatomy, Graduate School of Medicine, Professir, Prof lv
• Nov. 2012, Dec. 2018, Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo, Lecturer, Associate prof lecturer lv
• Mar. 2011, Nov. 2012, Assistant prof lv
• 2007, 2011, Harvard University Department of Molecular and Cellular Biology, Postdoctoral fellow, Researcher postdoc lv
• 2002, 2003, Researcher postdoc lv
• 2004, 2007, National Institute for Physiological Sciences, Research assistance
• 2004, 2005, Research assistance
• 2002, 2003, Research assistance
• 2002, RIKEN, Researcher postdoc lv

Research Activities

Research Areas

• Life sciences, Physiology
• Life sciences, Clinical pharmacy
• Life sciences, Anatomy
• Life sciences, Developmental biology
• Life sciences, Cell biology
• Life sciences, Biophysics
• Life sciences, Molecular biology
• Life sciences, Animals: biochemistry, physiology, behavioral science
• Life sciences, Morphology, anatomy
• Nanotechnology/Materials, Molecular biochemistry
• Life sciences, Neuroscience - general
• Life sciences, Neuroanatomy and physiology

Published Papers

• Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens, Nobukazu Ishii ,Yuki Tajika, Tohru Murakami , Josephine Galipon , Hiroyoshi Shirahata , Ryo Mukai , Daisuke Uehara , Ryosuke Kaneko ,YuichiYamazaki , Yuhei Yoshimoto, Hirohide Iwasak, 2021, Scientific Reports, 11, 13108, Scientific journal
• Doublecortin-like kinase enhances dendritic remodelling and negatively regulates synapse maturation., Euikyung Shin; Yutaro Kashiwagi; Toshihiko Kuriu; Hirohide Iwasaki; Teruyuki Tanaka; Hiroyuki Koizumi; Joseph G Gleeson; Shigeo Okabe, 2013, Nature communications, 4, 1440, 1440

  DOI
• Dlx1 transcription factor regulates dendritic growth and postsynaptic differentiation through inhibition of neuropilin-2 and PAK3 expression., Xiaojing Dai; Hirohide Iwasaki; Masahiko Watanabe; Shigeo Okabe, Feb. 2014, The European journal of neuroscience, 39, 4, 531, 47

  DOI
• Near-infrared deep brain stimulation via upconversion nanoparticle-mediated optogenetics., Shuo Chen; Adam Z Weitemier; Xiao Zeng; Linmeng He; Xiyu Wang; Yanqiu Tao; Arthur J Y Huang; Yuki Hashimotodani; Masanobu Kano; Hirohide Iwasaki; Laxmi Kumar Parajuli; Shigeo Okabe; Daniel B Loong Teh; Angelo H All; Iku Tsutsui-Kimura; Kenji F Tanaka; Xiaogang Liu; Thomas J McHugh, 09 Feb. 2018, Science (New York, N.Y.), 359, 6376, 679, 684, Scientific journal

  DOI
• Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscopy (FIB/SEM)., Ai Takahashi-Nakazato; Laxmi Kumar Parajuli; Hirohide Iwasaki; Shinji Tanaka; Shigeo Okabe, 2019, Methods in molecular biology (Clifton, N.J.), 1941, 17, 27, Scientific journal

  DOI
• A voltage-sensing phosphatase, Ci-VSP, which shares sequence identity with PTEN, dephosphorylates phosphatidylinositol 4,5-bisphosphate, Hirohide Iwasaki; Yoshimichi Murata; Youngjun Kim; Md. Israil Hossain; Carolyn A. Worby; Jack E. Dixon; Thomas McCormack; Takehiko Sasaki; Yasushi Okamura, Jun. 2008, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105, 23, 7970, 7975, Scientific journal

  DOI
• Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish voltage-sensing phosphatases, Md. Israil Hossain; Hirohide Iwasaki; Yoshifumi Okochi; Mohamed Chahine; Shinichi Higashijima; Kuniaki Nagayama; Yasushi Okamura, Jun. 2008, JOURNAL OF BIOLOGICAL CHEMISTRY, 283, 26, 18248, 18259, Scientific journal

  DOI
• 2-aminoethoxydiphenyl borate (2-APB) inhibits capacitative calcium entry independently of the function of inositol 1,4,5-trisphosphate receptors, H Iwasaki; Y Mori; Y Hara; K Uchida; H Zhou; K Mikoshiba, 2001, RECEPTORS & CHANNELS, 7, 6, 429, 439, Scientific journal
• Voltage-gated proton channel is expressed on phagosomes, Yoshifumi Okochi; Mari Sasaki; Hirohide Iwasaki; Yasushi Okamura, May 2009, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 382, 2, 274, 279, Scientific journal

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• Voltage-sensing phosphatase: actions and potentials, Yasushi Okamura; Yoshimichi Murata; Hirohide Iwasaki, Feb. 2009, JOURNAL OF PHYSIOLOGY-LONDON, 587, 3, 513, 520

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• Ca2+ induces macropinocytosis via F-actin depolymerization during growth cone collapse, Hiroyuki Kabayama; Takeshi Nakamura; Makoto Takeuchi; Hirohide Iwasaki; Masahiko Taniguchi; Naoko Tokushige; Katsuhiko Mikoshiba, Jan. 2009, MOLECULAR AND CELLULAR NEUROSCIENCE, 40, 1, 27, 38, Scientific journal

  DOI
• Ankyrin-G regulates inactivation gating of the neuronal sodium channel, Nav1.6, Emi Shirahata; Hirohide Iwasaki; Masahiro Takagi; Changqing Lin; Vann Bennett; Yasushi Okamura; Kiyoshi Hayasaka, Sep. 2006, JOURNAL OF NEUROPHYSIOLOGY, 96, 3, 1347, 1357, Scientific journal

  DOI
• 2-Aminoethyl diphenylborinate analogues: Selective inhibition for store-operated Ca2+ entry, Hong Zhou; Hirohide Iwasaki; Takeshi Nakamura; Kyoko Nakamura; Takayuki Maruyama; Shin-ichi Hamono; Shoichiro Ozaki; Akihiro Mizutani; Katsuhiko Mikoshiba, Jan. 2007, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 352, 2, 277, 282, Scientific journal

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• Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor, Y Murata; H Iwasaki; M Sasaki; K Inaba; Y Okamura, Jun. 2005, NATURE, 435, 7046, 1239, 1243, Scientific journal

  DOI
• Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor, Hirohide Iwasaki; Yoshimichi Murata; Mari Sasaki; Kazuo Inaba; Yasushi Okamura, Dec. 2005, ZOOLOGICAL SCIENCE, 22, 12, 1449, 1449, Scientific journal
• Comprehensive analysis of the ascidian genome reveals novel insights into the molecular evolution of ion channel genes, Y Okamura; A Nishino; Y Murata; K Nakajo; H Iwasaki; Y Ohtsuka; M Tanaka-Kunishima; N Takahashi; Y Hara; T Yoshida; M Nishida; H Okado; H Watari; IA Meinertzhagen; N Satoh; K Takahashi; Y Satou; Y Okada; Y Mori, Aug. 2005, PHYSIOLOGICAL GENOMICS, 22, 3, 269, 282, Scientific journal

  DOI
• High efficient expression of the functional ligand binding site of the inositol 1,4,5-trisphosphate receptor in Escherichia coli, F Yoshikawa; T Uchiyama; H Iwasaki; C Tomomori-Satoh; T Tanaka; T Furuichi; K Mikoshiba, Apr. 1999, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 257, 3, 792, 797, Scientific journal

  DOI
• Cooperative formation of the ligand-binding site of the inositol 1,4,5-trisphosphate receptor by two separable domains, F Yoshikawa; H Iwasaki; T Michikawa; T Furuichi; K Mikoshiba, Jan. 1999, JOURNAL OF BIOLOGICAL CHEMISTRY, 274, 1, 328, 334, Scientific journal

  DOI
• Trypsinized cerebellar inositol 1,4,5-trisphosphate receptor - Structural and functional coupling of cleaved ligand binding and channel domains, F Yoshikawa; H Iwasaki; T Michikawa; T Furuichi; K Mikoshiba, Jan. 1999, JOURNAL OF BIOLOGICAL CHEMISTRY, 274, 1, 316, 327, Scientific journal

  DOI
• Molecular characterization of the starfish inositol 1,4,5-trisphosphate receptor and its role during oocyte maturation and fertilization, H Iwasaki; K Chiba; T Uchiyama; F Yoshikawa; F Suzuki; M Ikeda; T Furuichi; K Mikoshiba, Jan. 2002, JOURNAL OF BIOLOGICAL CHEMISTRY, 277, 4, 2763, 2772, Scientific journal

  DOI
• 膜電位を感じて活性を変化させるイノシトールリン脂質脱リン酸化酵素, 岡村康司; 村田喜理; 岩崎広英, Jan. 2006, 51, 1, 18, 26, Scientific journal
• Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase II alpha and Rap1, Solveigh Cornelia Koeberle; Shinji Tanaka; Toshihiko Kuriu; Hirohide Iwasaki; Andreas Koeberle; Alexander Schulz; Dario-Lucas Helbing; Yoko Yamagata; Helen Morrison; Shigeo Okabe, Oct. 2017, SCIENTIFIC REPORTS, 7, 13409, Scientific journal

  DOI
• [New role of voltage sensor: voltage-regulated phosphatase recently identified from ascidian genome], Okamura Y; Murata Y; Iwasaki H; Sasaki M, Jan. 2006, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 51, 18, 26
• Comprehensive Profiling of Gene Expression in the Cerebral Cortex and Striatum of BTBRTF/ArtRbrc Mice Compared to C57BL/6J Mice, Shota Mizuno; Jun-na Hirota; Chiaki Ishii; Hirohide Iwasaki; Yoshitake Sano; Teiichi Furuichi, 10 Dec. 2020, Frontiers in Cellular Neuroscience, 14, Scientific journal

  DOI
• Geometry and the organizational principle of spine synapses along a dendrite, Laxmi Kumar Parajuli; Hidetoshi Urakubo; Ai Takahashi-Nakazato; Roberto Ogelman; Hirohide Iwasaki; Masato Koike; Hyung-Bae Kwon; Shin Ishii; Won Chan Oh; Yugo Fukazawa; Shigeo Okabe, 27 Oct. 2020, eneuro, ENEURO.0248, 20.2020, Scientific journal

  DOI
• Molecular assembly of excitatory synapses, Hirohide Iwasaki; Shinji Tanaka; Shigeo Okabe, 01 Jan. 2016, Dendrites: Development and Disease, 359, 385, In book

  DOI
• Comprehensive analysis of the ascidian genome reveals novel insights into the molecular evolution of ion channel genes, Yasushi Okamura; Atsuo Nishino; Yoshimichi Murata; Koichi Nakajo; Hirohide Iwasaki; Yukio Ohtsuka; Motoko Tanaka-Kunishima; Nobuyuki Takahashi; Yuji Hara; Takashi Yoshida; Motohiro Nishida; Haruo Okado; Hirofumi Watari; Ian A. Meinertzhagen; Nori Satoh; Kunitaro Takahashi; Yutaka Satou; Yasunobu Okada; Yasuo Mori, Oct. 2005, Physiological Genomics, 22, 269, 282, Scientific journal

  DOI
• Interaction between Teneurin-2 and microtubules via EB proteins provides a platform for GABAA receptor exocytosis., Ichinose Sotaro;Susuki Yoshihiro;Hosoi Nobutake;Kaneko Ryosuke;Ebihara Mizuho;Hirai Hirokazu;Iwasaki Hirohide, Jun. 2023, eLife, 12

  DOI
• Comprehensive Profiling of Gene Expression in the Cerebral Cortex and Striatum of BTBRTF/ArtRbrc Mice Compared to C57BL/6J Mice, Mizuno, Shota;Hirota, Jun-na;Ishii, Chiaki;Iwasaki, Hirohide;Sano, Yoshitake;Furuichi, Teiichi, 2020, FRONTIERS IN CELLULAR NEUROSCIENCE, 14

  DOI
• Geometry and the organizational principle of spine synapses along a dendrite, Parajuli, Laxmi Kumar;Urakubo, Hidetoshi;Takahashi-Nakazato, Ai;Ogelman, Roberto;Iwasaki, Hirohide;Koike, Masato;Kwon, Hyung-Bae;Ishii, Shin;Oh, Won Chan;Fukazawa, Yugo;Okabe, Shigeo, 2020, ENEURO, 7, 6

  DOI
• Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens, Ishii, Nobukazu;Tajika, Yuki;Murakami, Tohru;Galipon, Josephine;Shirahata, Hiroyoshi;Mukai, Ryo;Uehara, Daisuke;Kaneko, Ryosuke;Yamazaki, Yuichi;Yoshimoto, Yuhei;Iwasaki, Hirohide, 2021, SCIENTIFIC REPORTS, 11, 1

  DOI
• Insight into the function of a unique voltage-sensor protein (TMEM266) and its short form in mouse cerebellum, Kawai, Takafumi;Narita, Hirotaka;Konno, Kohtarou;Akter, Sharmin;Andriani, Rizki Tsari;Iwasaki, Hirohide;Nishikawa, Shoji;Yokoi, Norihiko;Fukata, Yuko;Fukata, Masaki;Wiriyasermkul, Pattama;Kongpracha, Pornparn;Nagamori, Shushi;Takao, Keizo;Miyakawa, Tsuyoshi;Abe, Manabu;Sakimura, Kenji;Watanabe, Masahiko;Nakagawa, Atsushi;Okamura, Yasushi, 2022, BIOCHEMICAL JOURNAL, 479, 11, 1127, 1145

  DOI
• Recent technological advances in correlative light and electron microscopy for the comprehensive analysis of neural circuits, Iwasaki, Hirohide;Ichinose, Sotaro;Tajika, Yuki;Murakami, Tohru, 2022, FRONTIERS IN NEUROANATOMY, 16

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• Editorial: Cutting-edge technologies for the comprehensive analysis of neural circuits, Iwasaki, Hirohide;Lee, Kea Joo, 2022, FRONTIERS IN NEUROANATOMY, 16

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• Correlative microscopy and block-face imaging (CoMBI): a 3D imaging method with wide applicability in the field of biological science, Tajika, Yuki;Ishii, Nobukazu;Morimura, Yoshihiro;Fukuda, Kyosuke;Shikada, Mitsuaki;Murakami, Tohru;Ichinose, Sotaro;Yoshimoto, Yuhei;Iwasaki, Hirohide, 2023, ANATOMICAL SCIENCE INTERNATIONAL, 98, 3, 353, 359

  DOI
• Interaction between Teneurin-2 and microtubules via EB proteins provides a platform for GABAA receptor exocytosis, Ichinose, Sotaro;Susuki, Yoshihiro;Hosoi, Nobutake;Kaneko, Ryosuke;Ebihara, Mizuho;Hirai, Hirokazu;Iwasaki, Hirohide;Ori-McKenney, Kassandra M., 2023, ELIFE, 12

  DOI
• Understanding the Principles of Neural Circuit Formation, Iwasaki Hirohide, Nov. 2020, The Kitakanto Medical Journal, 70, 4, 387, 388

  DOI
• Geometry and the Organizational Principle of Spine Synapses along a Dendrite., Parajuli Laxmi Kumar;Urakubo Hidetoshi;Takahashi-Nakazato Ai;Ogelman Roberto;Iwasaki Hirohide;Koike Masato;Kwon Hyung-Bae;Ishii Shin;Oh Won Chan;Fukazawa Yugo;Okabe Shigeo, Oct. 2020, eNeuro, 7, 6

  DOI
• Comprehensive Profiling of Gene Expression in the Cerebral Cortex and Striatum of BTBRTF/ArtRbrc Mice Compared to C57BL/6J Mice., Mizuno Shota;Hirota Jun-Na;Ishii Chiaki;Iwasaki Hirohide;Sano Yoshitake;Furuichi Teiichi, Dec. 2020, Frontiers in cellular neuroscience, 14

  DOI
• Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens., Ishii Nobukazu;Tajika Yuki;Murakami Tohru;Galipon Josephine;Shirahata Hiroyoshi;Mukai Ryo;Uehara Daisuke;Kaneko Ryosuke;Yamazaki Yuichi;Yoshimoto Yuhei;Iwasaki Hirohide, Jun. 2021, Scientific reports, 11, 1

  DOI
• Insight into the function of a unique voltage-sensor protein (TMEM266) and its short form in mouse cerebellum., Kawai Takafumi;Narita Hirotaka;Konno Kohtarou;Akter Sharmin;Andriani Rizki Tsari;Iwasaki Hirohide;Nishikawa Shoji;Yokoi Norihiko;Fukata Yuko;Fukata Masaki;Wiriyasermkul Pattama;Kongpracha Pornparn;Nagamori Shushi;Takao Keizo;Miyakawa Tsuyoshi;Abe Manabu;Sakimura Kenji;Watanabe Masahiko;Nakagawa Atsushi;Okamura Yasushi, The Biochemical journal, 479, 11

  DOI
• Recent technological advances in correlative light and electron microscopy for the comprehensive analysis of neural circuits., Iwasaki Hirohide;Ichinose Sotaro;Tajika Yuki;Murakami Tohru, Nov. 2022, Frontiers in neuroanatomy, 16

  DOI
• Editorial: Cutting-edge technologies for the comprehensive analysis of neural circuits., Iwasaki Hirohide;Lee Kea Joo, Dec. 2022, Frontiers in neuroanatomy, 16

  DOI
• Correlative microscopy and block-face imaging (CoMBI): a 3D imaging method with wide applicability in the field of biological science., Tajika Yuki;Ishii Nobukazu;Morimura Yoshihiro;Fukuda Kyosuke;Shikada Mitsuaki;Murakami Tohru;Ichinose Sotaro;Yoshimoto Yuhei;Iwasaki Hirohide, Feb. 2023, Anatomical science international, 98, 3

  DOI
• Interaction between Teneurin-2 and microtubules via EB proteins provides a platform for GABAA receptor exocytosis, Sotaro Ichinose; Yoshihiro Susuki; Nobutake Hosoi; Ryosuke Kaneko; Mizuho Ebihara; Hirokazu Hirai; Hirohide Iwasaki, 05 Jun. 2023, eLife, 12, e83276, Scientific journal

  DOI
• Correlative microscopy and block-face imaging (CoMBI): a 3D imaging method with wide applicability in the field of biological science, Yuki Tajika; Nobukazu Ishii; Yoshihiro Morimura; Kyosuke Fukuda; Mitsuaki Shikada; Tohru Murakami; Sotaro Ichinose; Yuhei Yoshimoto; Hirohide Iwasaki, 28 Feb. 2023, Anatomical Science International, Scientific journal

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• Editorial: Cutting-edge technologies for the comprehensive analysis of neural circuits, Hirohide Iwasaki; Kea Joo Lee, 05 Dec. 2022, Frontiers in Neuroanatomy, 16, Scientific journal

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• Recent technological advances in correlative light and electron microscopy for the comprehensive analysis of neural circuits, Hirohide Iwasaki; Sotaro Ichinose; Yuki Tajika; Tohru Murakami, 30 Nov. 2022, Frontiers in Neuroanatomy, 16, Scientific journal

  DOI
• Insight into the function of a unique voltage-sensor protein (TMEM266) and its short form in mouse cerebellum., Takafumi Kawai; Hirotaka Narita; Kohtarou Konno; Sharmin Akter; Rizki Tsari Andriani; Hirohide Iwasaki; Shoji Nishikawa; Norihiko Yokoi; Yuko Fukata; Masaki Fukata; Pattama Wiriyasermkul; Pornparn Kongpracha; Shushi Nagamori; Keizo Takao; Tsuyoshi Miyakawa; Manabu Abe; Kenji Sakimura; Masahiko Watanabe; Atsushi Nakagawa; Yasushi Okamura, 16 May 2022, The Biochemical journal, 479, 11, 1127, 1145, Scientific journal

  DOI
• Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens, Nobukazu Ishii; Yuki Tajika; Tohru Murakami; Josephine Galipon; Hiroyoshi Shirahata; Ryo Mukai; Daisuke Uehara; Ryosuke Kaneko; Yuichi Yamazaki; Yuhei Yoshimoto; Hirohide Iwasaki, 23 Jun. 2021, Scientific Reports, 11, 1, 13108, 13108, Scientific journal

  DOI

MISC

• イオンの出入りを介さず膜電位シグナルを伝えるタンパク質Ci-VSP, 岩崎広英; 村田喜理; 佐々木真理; 岡村康司, Dec. 2005, 23, 19, 2942, 2944, Introduction international proceedings
• [Diversity of the molecular mechanisms of synapse formation in the central nervous system]., Risa Iguchi; Hirohide Iwasaki; Shigeo Okabe, Feb. 2017, Seikagaku. The Journal of Japanese Biochemical Society, 89, 1, 77, 80
• Ca2+ INDUCES MACROPINOCYTOSIS VIA F-ACTIN DEPOLYMERIZATION DURING GROWTH CONE COLLAPSE, Hiroyuki Kabayama; Takeshi Nakamura; Makoto Takeuchi; Hirohide Iwasaki; Masahiko Taniguchi; Naoko Tokushige; Katsuhiko Mikoshiba, 2009, JOURNAL OF PHYSIOLOGICAL SCIENCES, 59, 337, 337, Summary international conference
• Analysis of a voltage-gated proton channel, VSOP in microglia, Yoshifumi Okochi; Makoto Sawada; Mari Sasaki; Hirohide Iwasaki; Yasushi Okamura, 2007, NEUROSCIENCE RESEARCH, 58, S187, S187, Summary international conference
• 自閉症モデルマウスBTBR系統で特異的に発現変動する遺伝子の脳内局在および機能解析, 弘田 淳奈; 水野 翔太; 岩崎 広英; 岡部 繁男; 佐野 良威; 古市 貞一, Sep. 2019, 92回, [3P, 326]
• Interactions between synapses and glial assembly in the cerebral cortex, 岩﨑 広英; 田中 慎二; 岡部 繁男, Nov. 2015, 66, 6, 531, 535
• 電子顕微鏡連続断面微細構造観察による3次元再構築イメージ解析 自動連続切片回収機ATUMを用いた神経回路解析, 岩崎 広英; 岡部 繁男, Jan. 2015, 日本生理学雑誌, 77, 1, 8, 9
• 【海馬とその周辺】 機能と分子基盤 海馬のシナプス維持とグリア細胞, 岩崎 広英; 田中 慎二; 岡部 繁男, Dec. 2013, Clinical Neuroscience, 31, 12, 1400, 1402
• W1-03 自動切片回収機ATUMを用いた神経回路のコネクトミクス的解析(W1 新しい3次元的電顕解析法と組織細胞化学の接点を探る,ワークショップ,第54回日本組織細胞化学会総会・学術集会), 岩崎 広英; 岡部 繁男, 2013, 54, 50, 50
• 2-aminoethoxydiphenyl borate(2-APB)のcapacitative calcium entryに対する効果, 岩崎 広英; 森 泰生; 原 雄二; 内田 敬子; 周 虹; 御子柴 克彦, Sep. 2001, 神経化学, 40, 2-3, 332, 332
• 2-aminoethoxydiphenyl borate(2-APB)のcapacitative calcium entryに対する阻害効果, 岩崎 広英; 森 泰生; 原 雄二; 内田 敬子; 周 虹; 御子柴 克彦, Aug. 2001, 生化学, 73, 8, 1018, 1018
• イトマキヒトデ卵成熟・受精過程におけるイノシトール3リン酸受容体の役割, 岩崎広英; 千葉和義; 内山剛; 吉川文生; 古市貞一; 御子柴克彦, 2000, 72, 8
• イオンチャネル研究の最前線 イノシトール3リン酸受容体チャネルの構造と生理機能, 道川 貴章; 廣田 順二; 山田 麻紀; 日野 美紀; 光山 冬樹; 岩崎 広英; 宮内 浩; 岩附 正人; 内山 剛; 内田 敬子; 横山 玲; 御子柴 克彦, Aug. 1999, 生化学, 71, 8, 594, 594
• Molecular cloning of starfish Inositol 1,4,5-Trisphosphate Receptor and its Role in oocyte maturation(Developmental Biology)(Proceedings of the Seventieth Annual Meeting of the Zoological Society of Japan) :, Iwasaki Hirohide; Chiba Kazuyoshi; Suzuki Fumiko; Ikeda Masako; Mikoshiba Katsuhiko, 1999, Zoological science, 16, 66, 66
• チャネル/トランスポータタンパクの構造,機能,疾病 IP3レセプターCa放出チャネルとその病態, 古市 貞一; 吉川 文生; 岩崎 広英; 平嶋 千恵; 水野 秀昭; 内山 剛; 井上 貴史; 岩附 正人; 松本 峰男; 日野 美紀, Aug. 1998, 70, 8, 681, 681

Books etc

• 脳神経回路と高次脳機能 スクラップ＆ビルドによる心の発達と脳疾患, Joint work, Aug. 2018
• "Dendrites: Development and Disease”, Joint work, Hirohide Iwasaki; Shinji Tanaka; Shige Okabe, Dendrites: Development and Disease”, Chapter 15. 'Molecular Assembly of Excitatory Synapses, Springer, Aug. 2016

Presentations

• Mechanism to regulate inhibitory synapse maturation via dynamic microtubules, Hirohide Iwasaki, The 99th Annual Meeting of the Physiological Society of Japan, 18 Mar. 2022, 16 Mar. 2022, 18 Mar. 2022, English, Japan, Domestic conference

Research Projects

• Molecular mechanism of inhibitory synapse formation, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Gunma University, 01 Apr. 2022, 31 Mar. 2025, 22K06805

  対象リソースIRI
• Ultrastructural analysis of synapse stability, Iwasaki Hirohide, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Gunma University, 01 Apr. 2018, 31 Mar. 2021, This project focused on the molecular mechanism of synaptic stabilization. First, dendritic spines of cortical pyramidal neurons were imaged in vivo using two-photon microscopy over time to identify stably maintained spines and newly emerging spines. The same dendrites were then examined by electron microscopy to obtain ultrastructural images of dendritic spines by three-dimensional reconstruction. The results showed that the neck length of the stable spines was almost the same as that of the newly emerged spines, but the spine head of the stable spines was larger and the morphology more complex. In addition, to elucidate the molecular mechanism of synaptic stabilization, we clarified the role of teneurin, a membrane protein involved in synaptic induction., 18K06499

  対象リソースIRI
• Structural Analysis of glial cells by using high resolution imaging method, Iwasaki Hirohide; OKABE Shigeo, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), The University of Tokyo, 01 Apr. 2015, 31 Mar. 2018, In the central nervous system of the mammalian brain, three types of glial cells have been identified; astrocyte, microglia and oligodendrocyte. Among them, oligodendrocyte forms myelin around axons. Other types of glial cells might contribute to the synaptic functions of the brain by affecting the synapse formation/elimination. In this research, I analyzed the precise structure of astrocyte and microglia by using CLEM (Correlative anaylysis of light- and electron- microscopy) method. Particularly, I succeeded the comprehensive three-dimensional reconstruct of the fine protrusions of single microglia., 15K06734

  対象リソースIRI
• Dynamics of neural circuit and its impariment studied by optical imaging, Okabe Shigeo; IWASAKI Hirohide, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), The University of Tokyo, 01 Apr. 2014, 31 Mar. 2017, This study aimed at understanding biological mechanism of synaptic dynamics by cutting-edge imaging technologies. To achieve this goal, we analyzed morphology and function of excitatory and inhibitory synapses in the neocortex, maturation steps of individual synapses imaged in vivo by two-photon microscopy, and defects in synaptic functions and dynamics in mouse models of psychiatric disorders. From these experiments, we conclude that synaptic dynamics in the developing neural circuits show circuit-specific regulation mechanisms. Furthermore, we showed that early impairments in synaptic dynamics cause dysfunction of neural circuits in later stages of development., 26250014

  対象リソースIRI
• Structural analysis of excitatory synapse by using high-resolution imaging method, IWASAKI Hirohide; OKABE Shigeo, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), The University of Tokyo, 01 Apr. 2012, 31 Mar. 2015, Neurons are connected via synapses to form neural circuits. In order to understand how neurons are involved in various brain functions at neural circuit level, it will be useful to analyze the wiring diagram of the brain at synapse level with high-resolution imaging technique which can cover large area. From this point of view, I established the hybrid method of light and electron microscopy, Array Tomography, for the three-dimensional reconstruction of neural tissue and synapse localization. I also established the method to observe synapses by using scanning electron microscopy. By using these technologies, I analyzed the morphology of excitatory synapses at developmental stage of mice., 24500405

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• 01 Apr. 2012, 31 Mar. 2014, 24120508

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• Molecular Anatomy of Synaptic Structure, OKABE Shigeo; TANAKA Shinji; IWASAKI Hirohide; KONDO Satoru; NISHII Kiyomasa, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (S), The University of Tokyo, 11 May 2009, 31 Mar. 2014, PALM and STORM are superresolution imaging techniques based on detection of single fluorescent molecules. Although postsynaptic densities (PSDs) are disc-like microstructures with their diameters of less than 500 nm, PALM and STORM are able to resolve their internal structure. In this study, fluorescently labeled PSDs and spines were visualized by using PALM and the molecular distributions within these structures were detected. Furthermore, we could successfully detected distributional changes of PSD molecules during development of synapses and after induction of synaptic plasticity., 21220008

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• 24 Aug. 2011, 31 Mar. 2013, 23800018

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• Mechanisms and physiological roles of voltage-sensing phosphatase, OKAMURA Yasushi; HIGASHIJIMA Shinichi; OKOCHI Yoshifumi; IWASAKI Hirohide, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Priority Areas, 2006, 2010, Voltage sensing phosphatase gene is conserved in genomes from sea squirt to human. VSP consists of the voltage sensing domain and the cytoplasmic enzyme region that operates as the phosphoinositide phosphatase. Mechanisms of VSP have been examined by integrative approaches including electrophysiology, in vitro phosphatase assay, mathematical modeling analysis and imaging analysis. Results showed that voltage sensor activates PI(4,5)P2 and PIP3 phosphatase activity upon depolarization and this coupling occurs over the entire range of voltage sensitivity of the voltage sensor. We also found that the state of enzyme affects the motions of the voltage sensor suggesting the presence of retrograde interaction from enzyme to the voltage sensor., 18077013

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• Novel pathways of electro-chemical signals at membrane proteins, OKAMURA Yasushi; HIGASHIJIMA Shinichi; OKOCHI Yoshifumi; IWASAKI Hirohide; SASAKI Mari; FUJIWARA Yuichiro, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), 2006, 2008, 18209007

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• 2006, 2007, 18700372

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• 2006, 2007, 18059034

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• 2005, 2006, 17659065

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• 2005, 2005, 17024071

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• 2004, 2005, 17018043

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• 2004, 2005, 16700331

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• Mechanism of expression of voltage-gated sodium and calcium channels, OKAMURA Yasushi; KUKITA Fumio; IWASAKI Hirohide; EBIHARA Tatsuhiko; HAYASAKA Kiyoshi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), National Institute of Natural Sciences Okazaki Research Facilities, 2004, 2005, Ankyrin-G, a modular protein, plays a critical role in clustering voltage-gated sodium channels (Nav channels) in nodes of Ranvier and initial segments of mammalian neurons. However, direct effects of ankyrin-G on electrophysiological properties of Nav channels remai elusive. In this study, we explored whether ankyrin-G has a role in modifying gating properties of the neuronal Nav1.6 channel that is predominantly localized at nodes of Ranvier and initial segments. TsA201 cells transfected with the human Nav1.6 cDNA alone exhibited significant persistent sodium current (Ina-p). On the other hand, Ina-p was barely detected upon co-expression with ankyrin-G. Ankyrin-B, another ankyrin, did not show such an effect. Expression of chimeras between the two isofrms of ankyrin suggests that the membrane-binding domain of ankyurin-G is critical for reducing the Ina-p of Nav1.6. These results suggest that ankyrin-G regulates neuronal excitability not only through clustering Nav channels but also by directly modifying their channel gating. Changes is membraine potential affect ion channels, and transporters, which then alter intracellular chemical conditions. Ohter signaling pathways coupled to membrane potential have been suggested but their underlying mechanism are unknown. We have identified a novel protein from the ascidian Ciona intestinalis which has a transmembrane voltage sensing domain homologous to the S1-S4 segments of voltage-gated channels and a cytoplasmic domain similar to phosphatase and tension homologue (PTEN). This protein, named Ci-VSP (voltage-sensor containing phosphatase), displays channel-like "gating" currents and directly translates changes in membrane potential into the turnover of phosphoinositides. The activity of the phosphoinositide phosphatase in Ci-VSP is tuned within a physiological range of membrane potential. Immunocytochemical studies show that Ci-VSP is expressed in Ciona sperm tail membranes, suggesting a role in sperm function or morphology. Our data demonstrate that voltage sensing can function beyond channel proteins and thus more ubiquitously than previously appreciated., 16390056

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• 2004, 2004, 16015316

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• 2003, 2004, 15659054

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• 2003, 2003, 15016107

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• 2002, 2003, 14780588

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• Molecular mechanisms underlying inhibition of full-length protein expression by interaction, with truncated protein of Cav channel, OKAMURA Yasushi; IWASAKI Hirohide, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Okazaki National Research Institute, 2002, 2003, Truncated calcium channel subunits have been reported in developing tissue or in human channelopaties. However, functional significance of such truncated versions of proteins remain unknown. We studied molecular mechanisms underlying inhibition of expression of full length proteins by truncated proteins in Xenopus oocyte and BHK-G cells. Two electrode voltage clamp and whole cell patch clamp methods were applied on those cells. When truncated mammalian Cav1.2 subunit was coexpressed with the full length cDNA, remarkable suppression of channel expression was observed. This effect occurred at protein level, since introduction of premature stop codon eliminated this suppression. By Western blot analysis, it was shown that truncated protein is transferred into insoluble membrane fraction. Interestingly, the full length protein was also transferred to this fraction on coexpression with the truncated protein, indicating that misfolding of the truncated protein integrates the full length protein via unknown interactions and degradation or suppression of protein synthesis occurs. We also found that some truncated forms of voltage gated sodium channels show dominant-negative effects, suggesting that inhibitory mechanisms of truncated proteins are conserved among 4-motif type voltage gated channels., 14370017

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• 2002, 2002, 14017092

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