Researcher Profile and Settings

Researcher

• Name

  SEKIMOTO Takayuki

Profile and Settings

• Name

  Sekimoto, Takayuki

Website

• URI

  http://makukinou.showa.gunma-u.ac.jp/index.html, Laboratory of Molecular Membrane Biology

Affiliation

• Institute for Molecular and Cellular Regulation, Gunma University, Laboratory of Molecular Membrane Biology, Assistant Professor

Education

• Apr. 2002, Mar. 2006, Kanazawa University, Graduate School of Natural Science and Technology, Division of Life Sciences
• Apr. 1995, Mar. 1999, Kanazawa University, Faculty of Science, Department of Biology
• Apr. 1999, Mar. 2002, Kanazawa University, National Science and Technology

Degree

• Doctor of Science

Association Memberships

• The Molecular Biology Society of Japan, Jul. 2007, 9999
• Japanese Cancer Association, Jun. 2010, 9999

Research Experience

• Mar. 2021, 9999, Institute for Molecular and Cellular Regulation, Gunma University, Laboratory of Molecular membrane biology, Assistant Professor, Assistant prof lv
• Jul. 2006, Mar. 2008, Institute for Molecular and Cellular Regulation, Gunma University, Laboratory of Molecular genetics, Postdoctoral fellow, Researcher postdoc lv
• Apr. 2008, Apr. 2010, Institute for Molecular and Cellular Regulation, Gunma University, Laboratory of Molecular genetics, Postdoctoral fellow, Researcher postdoc lv
• May 2010, Feb. 2021, Institute for Molecular and Cellular Regulation, Gunma University, Laboratory of Molecular genetics, Assistant Professor, Assistant prof lv

Research Activities

Research Areas

• Life sciences, Molecular biology, Molecular cell biology
• Life sciences, Tumor diagnostics and therapeutics, molecular oncology

Research Interests

• Heat shock response
• DNA damage response
• Oncogene induced replication stress
• Oncogenesis
• Cell senescence
• DNA repair
• DNA damage
• genomic instability
• Translesion DNA synthesis
• Y-family Polymerase

Research Themes

• Personal, 2010, -, General Medical Chemistry, Grant-in-Aid for Scientific Research

Published Papers

• Coordinate responses of transcription factors to ecdysone during programmed cell death in the anterior silk gland of the silkworm, Bombyx mori, T. Sekimoto; M. Iwami; S. Sakurai, Jun. 2006, Insect Molecular Biology, 15, 3, 281, 292, Scientific journal

  DOI
• Hsp90 and the Fanconi anemia pathway - A molecular link between protein quality control and the DNA damage response, Yamashita, Takayuki;Oda, Tsukasa;Sekimoto, Takayuki, 2007, CELL CYCLE, 6, 18, 2232, 2235
• 20-Hydroxyecdysone regulation of two isoforms of the Ets transcription factor E74 gene in programmed cell death in the silkworm anterior silk gland, T. Sekimoto; M. Iwami; S. Sakurai, Sep. 2007, Insect Molecular Biology, 16, 5, 581, 590, Scientific journal

  DOI
• Identification, characterization, and developmental regulation of two storage proteins in the bamboo borer Omphisa fuscidentalis, Jatuporn Tungjitwitayakul; Tippawan Singtripop; Anchalee Nettagul; Yasunori Oda; Nujira Tatun; Takayuki Sekimoto; Sho Sakurai, Jan. 2008, Journal of Insect Physiology, 54, 1, 62, 76, Scientific journal

  DOI
• The Molecular Chaperone Hsp90 Regulates Accumulation of DNA Polymerase η at Replication Stalling Sites in UV-Irradiated Cells, Takayuki Sekimoto; Tsukasa Oda; Franklin Mayca Pozo; Yoshiki Murakumo; Chikahide Masutani; Fumio Hanaoka; Takayuki Yamashita, Jan. 2010, Molecular Cell, 37, 1, 79, 89, Scientific journal

  DOI
• Molecular Chaperone Hsp90 Regulates REV1-Mediated Mutagenesis, F. Mayca Pozo; T. Oda; T. Sekimoto; Y. Murakumo; C. Masutani; F. Hanaoka; T. Yamashita, Aug. 2011, Molecular and Cellular Biology, 31, 16, 3396, 3409, Scientific journal

  DOI
• Both High-Fidelity Replicative and Low-Fidelity Y-Family Polymerases Are Involved in DNA Rereplication, Takayuki Sekimoto; Tsukasa Oda; Kiminori Kurashima; Fumio Hanaoka; Takayuki Yamashita, Feb. 2015, Molecular and Cellular Biology, 35, 4, 699, 715, Scientific journal

  DOI
• Acute HSF1 depletion induces cellular senescence through the MDM2-p53-p21 pathway in human diploid fibroblasts, Tsukasa Oda; Takayuki Sekimoto; Kiminori Kurashima; Mitsuaki Fujimoto; Akira Nakai; Takayuki Yamashita, May 2018, Journal of Cell Science, 131, 9, jcs210724, jcs210724, Scientific journal

  DOI
• Polη, a Y-family translesion synthesis polymerase, promotes cellular tolerance of Myc-induced replication stress, Kiminori Kurashima; Takayuki Sekimoto; Tsukasa Oda; Tsuyoshi Kawabata; Fumio Hanaoka; Takayuki Yamashita, Jun. 2018, Journal of Cell Science, 131, 12, jcs212183, jcs212183, Scientific journal

  DOI

MISC

• Hsp90 and the Fanconi Anemia Pathway: A Molecular Link Between Protein Quality Control and the DNA Damage Response, Takayuki Yamashita; Tsukasa Oda; Takayuki Sekimoto, 15 Sep. 2007, Cell Cycle, 6, 18, 2232, 2235, Introduction scientific journal

  DOI
• Fanconi貧血―ゲノム損傷ストレスと造血幹細胞の老化―, 山下孝之; 小田司; 関本隆志, Mar. 2008, 66, 3, 477, 482, Introduction commerce magazine
• Fanconi貧血の分子病態―最近の進歩, 山下孝之; 小田司; 関本隆志, Jul. 2009, 50, 7, 538, 546, Introduction scientific journal

  DOI
• 前がん病変における細胞老化とゲノム不安定性, 関本 隆志, 2013, 27, 1-5.
• Translesion DNA Synthesis and Hsp90, Takayuki Yamashita; Tsukasa Oda; Takayuki Sekimoto, May 2012, Genes and Environment, 34, 2, 89, 93, Introduction scientific journal

  DOI
• Y-family polymerases are involved in oncogene-induced aberrant replication, Takayuki Sekimoto; Tsukasa Oda; Kiminori Kurashima; Fumio Hanaoka; Takayuki Yamashita, Apr. 2015, DNA REPAIR, 28, 144, 144, Summary international conference

Presentations

• がん遺伝c-Mycが誘導する複製ストレスにおけるグアニン四重鎖構造の役割, 関本隆志, 第9回 DNA損傷ワークショップ, 04 Jul. 2022, 04 Jul. 2022, 05 Jul. 2022, Japanese, Japan, Domestic conference
• がん遺伝子Mycが誘導する複製ストレスにおけるグアニン四重鎖の役割, 関本隆志, Genome Damage Network Workshop 2021 ~Online Retreat~, 18 Nov. 2021, 18 Nov. 2021, 09 Nov. 2121, English
• Myc誘導性複製ストレスにおけるグアニン四重鎖構造は治療標的になり得るか, 関本 隆志, 第80回 日本癌学会学術総会, 30 Sep. 2021, 30 Sep. 2021, 02 Oct. 2021, English, Japan, Domestic conference
• 分子シャペロンHsp90による損傷乗り越えDNA合成の制御, 関本隆志, Global COE Program: Signal Transduction in the Regulatory System and Its Disorders 第2回若手研究者シンポジウム, 19 Jul. 2008, 18 Jul. 2008, 19 Jul. 2008, Japanese
• 分子シャペロンHsp90は損傷乗り越えDNA合成に関与するポリメラーゼPol-η (eta)の働きを制御する, 関本隆志, Global COE Program: Signal Transduction in the Regulatory System and Its Disorders 第3回若手研究者シンポジウム, 10 Nov. 2009, 10 Nov. 2009, 11 Nov. 2009, Japanese
• 分子シャペロンHsp90はTranslesion DNA synthesis (TLS) に関与するDNA polymerase-eta (Pol-η)のReplication focus (RF) への動員に必要である, 関本隆志; 小田司; 益谷央豪; 花岡文雄; 山下孝之, 第30回日本分子生物学会, 13 Dec. 2007, 11 Dec. 2007, 15 Dec. 2007, Japanese
• 熱ショック応答転写因子Heat shock factor 1(HSF1)の発現抑制はhTERT不死化ヒト細胞においてp53-p21を介する細胞老化を引き起こす, 小田司; 関本隆志; Mayca Pozo Franklin; 山下孝之, 第32回日本分子生物学会, 09 Dec. 2009, 09 Dec. 2009, 12 Dec. 2009, Japanese
• 分子シャペロンHsp90はPolymerase-η (Pol-η)の複製フォーカスへの集積と損傷乗越えDNA合成(TLS)を促進する, 関本隆志; 小田司; Mayca Pozo Franklin; 村雲芳樹; 益谷央豪; 花岡文雄; 山下孝之, 第31回日本分子生物学会, 12 Dec. 2008, 09 Dec. 2008, 12 Dec. 2008, Japanese
• 分子シャペロンHSP90はY-family DNAポリメラーゼREV1のDNA損傷による核内フォーカス形成を促進する, Mayca Pozo Franklin; 小田司; 関本隆志; 益谷央豪; 花岡文雄; 村雲芳樹; 山下孝之, 第32回日本分子生物学会, 09 Dec. 2009, 09 Dec. 2009, 12 Dec. 2009, English
• 熱ショック転写因子HSF1の急性欠乏は腫瘍抑制性の細胞老化プログラムを活性化する, 小田司; 関本隆志; 山下孝之, 第69回日本癌学会学術総会, 23 Sep. 2010, 22 Sep. 2010, 24 Sep. 2010, English
• 分子シャペロンHsp90はREV1による突然変異の誘発を制御する, Mayca Pozo Franklin; 小田司; 関本隆志; 村雲芳樹; 益谷央豪; 花岡文雄; 山下孝之, 第70回日本癌学会学術総会, 05 Oct. 2011, 03 Oct. 2011, 05 Oct. 2011, English
• 熱ショック転写因子HSF1の急性欠乏は複数の腫瘍抑制経路を介して細胞老化プログラムを活性化する, 小田司; 関本隆志; 山下孝之, 第70回日本癌学会学術総会, 04 Oct. 2011, 03 Oct. 2011, 05 Oct. 2011, English
• 熱ショック応答転写因子Heat shock factor 1 (HSF1)の発現抑制p53-p21経路を介した細胞老化を誘導する, 小田司; 関本隆志; 山下孝之, 第34回日本分子生物学会, 14 Dec. 2011, 13 Dec. 2011, 16 Dec. 2011, English
• ポリメラーゼηは発がんシグナルが誘導するDNA再複製とDNA損傷応答に関与する, 関本隆志; 小田司; 益谷央豪; 花岡文雄; 山下孝之, 第71回日本癌学会学術総会, 19 Sep. 2012, 19 Sep. 2012, 21 Sep. 2012, English
• 熱ショック応答転写因子Heat shock factor 1(HSF1)の抑制による細胞老化の誘導機構：DHRS2とp300/CBPの関与, 小田司; 関本隆志; 山下孝之, 第35回日本分子生物学会, 12 Dec. 2012, 11 Dec. 2012, 14 Dec. 2012, English
• Y-family DNAポリメラーゼは、発がんシグナルが誘導するDNA再複製に関与する, 関本隆志; 小田司; 益谷央豪; 花岡文雄; 山下孝之, 第35回日本分子生物学会, 13 Dec. 2012, 11 Dec. 2012, 14 Dec. 2012, English
• Y-family DNAポリメラーゼによる損傷乗り越えDNA合成は、cyclin E過剰発現によるDNA複製ストレス応答に関与する, 関本隆志; 小田司; 益谷央豪; 花岡文雄; 山下孝之, 第34回日本分子生物学会, 15 Dec. 2011, 13 Dec. 2011, 16 Dec. 2011, English
• Myc誘導性複製ストレス応答においてPolηとMus81-EME2の二重阻害は合成致死を示す, 倉島公憲; 関本隆志; 小田司; 花岡文雄; 山下孝之, 第76回日本癌学会学術総会, 28 Sep. 2017, 28 Sep. 2017, 30 Sep. 2017, English
• DNA架橋剤メルファランは多発性骨髄腫細胞株において主要組織適合抗原クラスII(MHCII)の転写活性化因子CIITAの発現を促進する, 中村瑠里; 小田司; 関本隆志; 松田美弥子; 大圃真澄; 半田寛; 笠松哲光; 斎藤貴之; 村上博和; 山下孝之, 第40回日本分子生物学会, 07 Dec. 2017, 06 Dec. 2017, 09 Dec. 2017, Japanese
• Y-familyポリメラーゼPolηはMus81/EME2ヌクレアーゼ複合体と協同してがん遺伝子c-Mycによるreplication stress(RS)を緩和する, 関本隆志; 倉島公憲; 小田司; 川端剛; 松田美弥子; 中村瑠璃; 大圃真澄; 花岡文雄; 山下孝之, 第40回日本分子生物学会, 06 Dec. 2017, 06 Dec. 2017, 09 Dec. 2017, Japanese
• Yファミリー損傷乗り越えポリメラーゼPolηはc-MYC誘導性複製ストレスを軽減する, 倉島公憲; 関本隆志; 小田司; 花岡文雄; 山下孝之, 第75回日本癌学会学術総会, 08 Oct. 2016, 06 Oct. 2016, 08 Oct. 2016, English
• Y-familyポリメラーゼPolηとエンドヌクレアーゼMus81-Eme2複合体は段階的に協調してc-MYCがん遺伝子誘導性複製ストレスを抑制する, 倉島公憲; 関本隆志; 小田司; 花岡文雄; 山下孝之, 第39回日本分子生物学会, 02 Dec. 2016, 30 Nov. 2016, 02 Dec. 2016, English
• 熱ショック応答転写因子Heat shock factor 1 (HSF1)の発現抑制による細胞老化はMDM2阻害蛋白Dehycrogenase/reductase2 (DHRS2)に制御されている可能性がある, 小田司; 関本隆志; 倉島公憲; 山下孝之, 第39回日本分子生物学会, 01 Dec. 2016, 30 Nov. 2016, 02 Dec. 2016, English
• c-MycによるDNA複製ストレスにDNAポリメラーゼηが関与する, 倉島公憲; 小田司; 関本隆志; 小林広美; 尤礼佳; 花岡文雄; 山下孝之, 第36回日本分子生物学会, 05 Dec. 2013, 03 Dec. 2013, 06 Dec. 2013, English
• DHRS2は、熱ショック転写因子HSF1の発現抑制で誘導される細胞老化に関与する可能性がある, 小田司; 関本隆志; 倉島公憲; 山下孝之, 第36回日本分子生物学会, 04 Dec. 2013, 03 Dec. 2013, 06 Dec. 2013, English
• ポリメラーゼηは、発がんシグナルが誘導するDNA再複製に関与する, 関本隆志; 小田司; 倉島公憲; 花岡文雄; 山下孝之, 第36回日本分子生物学会, 05 Dec. 2013, 03 Dec. 2013, 06 Dec. 2013, English
• DHRS2は熱ショック転写因子HSF1抑制で誘導される細胞老化に関与する, 小田司; 関本隆志; 山下孝之, 第72回日本癌学会学術総会, 03 Oct. 2013, 03 Oct. 2013, 05 Oct. 2013, English
• Y-familyポリメラーゼは、発がんシグナルが誘導するDNA再複製に関与する, 関本隆志; 小田司; 益谷央豪; 花岡文雄; 山下孝之, 第72回日本癌学会学術総会, 04 Oct. 2013, 03 Oct. 2013, 05 Oct. 2013, English
• DNAポリメラーゼη(Polη)はc-mycによる複製ストレスを抑制する, 倉島公憲; 小田司; 関本隆志; 花岡文雄; 山下孝之, 第73回日本癌学会学術総会, 27 Sep. 2014, 25 Sep. 2014, 27 Sep. 2014, English
• 熱ショック転写因子HSF1の阻害はRasV12で形質転換した細胞の老化プログラムを選択的に活性化する, 小田司; 関本隆志; 倉島公憲; 山下孝之, 第73回日本癌学会学術総会, 27 Sep. 2014, 25 Sep. 2014, 27 Sep. 2014, English
• Y-ファミリーDNAポリメラーゼの一つであるPolηはc-mycにより誘導されるDNA二本鎖切断の生成を抑制する, 倉島公憲; 関本隆志; 小田司; 花岡文雄; 山下孝之, 第74回日本癌学会学術総会, 08 Oct. 2015, 08 Oct. 2015, 10 Oct. 2015, English
• HSF1抑制は細胞依存的なメカニズムで老化を誘導する, 小田司; 関本隆志; 倉島公憲; 山下孝之, 第74回日本癌学会学術総会, 08 Oct. 2015, 08 Oct. 2015, 10 Oct. 2015, English
• Y-family損傷乗り越えDNAポリメラーゼ(Y-Pol)の一員PolηはMYCがん遺伝子の誘導する複製ストレスを軽減する, 倉島公憲; 関本隆志; 小田司; 川端剛; 花岡文雄; 山下孝之, 第38回日本分子生物学会, 02 Dec. 2015, 01 Dec. 2015, 04 Dec. 2015, English
• Heat shock factor 1 (HSF1)抑制はDNA損傷および蛋白変性ストレスと独立して細胞老化を誘導する, 小田司; 関本隆志; 倉島公憲; 山下孝之, 第38回日本分子生物学会, 03 Dec. 2015, 01 Dec. 2015, 04 Dec. 2015, English
• HSF1抑制はDHRS2-MDM2-p53経路を介してヒト線維芽細胞に老化を誘導する, 小田司; 関本隆志; 倉島公憲; 山下孝之, 第76回日本癌学会学術総会, 30 Sep. 2017, 28 Sep. 2017, 30 Sep. 2017, English
• MDM2阻害因子Dehycrogenase/reductase2 (DHRS2)の細胞老化における役割, 小田司; 関本隆志; 倉島公憲; 中村瑠里; 松田美弥子; 大圃真澄; 山下孝之, 第40回日本分子生物学会, 06 Dec. 2017, 06 Dec. 2017, 09 Dec. 2017, Japanese
• HSF1抑制はタンパク質毒性ストレス非依存的に細胞老化を誘導する, 小田司; 関本隆志; 倉島公憲; 山下孝之, 第75回日本癌学会学術総会, 08 Oct. 2016, 06 Oct. 2016, 08 Oct. 2016, English
• A Molecular Chaperone Hsp90 Promotes Accumulation of DNA Polmerase η (Pol-η) to Replication Arrest Sites in UV-irradiated Cells, Takayuki Sekimoto; Tsukasa Oda; Mayca Pozo Franklin; Yoshiki Murakumo; Chikahide Masutani; Fumio Hanaoka; Takayuki Yamashita, Global COE International Symposium "The 8th International Conference on Protein Phosphatases", Nov. 2008, 12 Nov. 2008, 14 Nov. 2008, English
• 53BP1はHSF1抑制で誘導される細胞老化に関与する, 小田司; 関本隆志; 山下孝之, 第77回日本癌学会学術総会, 27 Sep. 2018, 27 Sep. 2018, 29 Sep. 2018, Japanese
• がん遺伝子誘導性Replication Stress(RS)への応答におけるRAD51を介するフォーク保護機構の役割, 大圃真純; 関本隆志; 熊谷理穂; 廣江珠希; 齋藤貴之; 村上博和; 山下孝之, 第41回日本分子生物学会年会, 30 Nov. 2018, 28 Nov. 2018, 30 Nov. 2018, English
• Myc誘導性複製ストレスにおけるグアニン四重鎖構造は治療標的になり得るか, 関本隆志; 山下孝之, 第43回日本分子生物学会, 03 Dec. 2020, 02 Dec. 2020, 04 Dec. 2020, English, Domestic conference
• がん遺伝子誘導性複製ストレスにおけるグアニン四重鎖構造の役割, 関本隆志; 山下孝之, 第42回日本分子生物学会, 04 Dec. 2019, 03 Dec. 2019, 06 Dec. 2019, English, Domestic conference
• Polη, a Y-family translesion synthesis polymerase, promotes cellular tolerance of Myc-induced replication stress, Takayuki Sekimoto; Kiminori Kurashima; Tsukasa Oda; Tsuyoshi Kawabata; Fumio Hanaoka; Takayuki Yamashita, The 4th IMCR Symposium on Endocrine and Metabolism: At the Cutting Edge of Metabolic Regulation Research, 08 Nov. 2018, 08 Nov. 2018, 09 Nov. 2018, English, International conference
• Y-familyポリメラーゼはがん遺伝子が誘導するDNA再複製に関与する, 関本隆志, 第3回生体情報研究シンポジウム〜グローバルCOEプログラム｢生体調節シグナルの統合的研究｣の新展開〜, 07 Aug. 2014, 07 Aug. 2014, Japanese
• ポリメラーゼηはがん遺伝子が誘導するDNA再複製に関与する, 関本隆志; 小田司; 倉島公憲; 花岡文雄; 山下孝之, ｢がん研究分野の特性等を踏まえた支援活動｣公開シンポジウム, 30 Jan. 2014, 30 Jan. 2014, 31 Jan. 2014, Japanese
• 損傷乗り越えDNA合成ポリメラーゼ制御機構, 関本隆志, Global-COE 若手研究者発表会, 27 Mar. 2008, 27 Mar. 2008, Japanese
• Genomic and nongenomic actions of 20E in programmed cell death of Bombyx anterior silk gland, Masatoshi Iga; Takayuki Sekimoto; Mohamed Elmogy; Masafumi Iwami; Sho Sakurai, 7th International Workshop on Molecular Biology and Genetics of the Lepidoptera, Aug. 2006, 20 Aug. 2006, 26 Aug. 2006, English
• Identification and characterization of two storage proteins in diapause larvae of the bamboo borer, Omphisa fuscidentalis, Jatuporn Tungjitwitayakul; Yasunori Oda; Nujira Tatun; Yu Kaneko; Takayuki Sekimoto; Tippawan Singtripop; Sho Sakurai, 7th International Workshop on Molecular Biology and Genetics of the Lepidoptera, Aug. 2006, 20 Aug. 2006, 26 Aug. 2006, English
• Interaction of genomic and nongenomic actions of 20-hydroxyecdysone in 20E-dependent development events, Masatoshi Iga; Takayuki Sekimoto; Mohamed Elmogy; Masafumi Iwami; Sho Sakurai, 16th International Ecdysone Workshop, Jul. 2006, 10 Jul. 2006, 14 Jul. 2006, English
• カイコガ前部絹糸腺の予定細胞死におけるステロイドホルモン受容体, 関本隆志; 岩見雅史; 桜井勝, 2005年 日本動物学会中部支部会, 30 Jul. 2005, 29 Jul. 2005, 30 Jul. 2005, Japanese
• カイコガ前部絹糸腺の予定細胞死における初期・初期後期遺伝子の発現動態, 関本隆志; 岩見雅史; 桜井勝, 日本動物学会第75回大会, Sep. 2004, 10 Sep. 2004, 12 Sep. 2004, Japanese
• Genomic & non-genomic action of ecdysteroid: both are required for completion of programmed cell death in the anterior silk gland, Mohamed El-Mogy; Masatoshi Iga; Takayuki Sekimoto; Masafumi Iwami; Sho Sakurai, XXII International Congress of Entomology, Aug. 2004, Aug. 2004, Aug. 2004, English
• カイコガ前部絹糸腺の予定細胞死における初期・初期後期遺伝子の発現動態, 関本隆志; 岩見雅史; 桜井勝, 日本動物学会第74回大会, Sep. 2003, 17 Sep. 2003, 19 Sep. 2003, Japanese
• カイコガ翅成虫原基の20E応答能獲得と初期遺伝子群の発現解析, 小山貴司; 関本隆志; 岩見雅史; 桜井勝, 日本動物学会第74回大会, Sep. 2003, 17 Sep. 2003, 19 Sep. 2003, Japanese
• カイコガ前部絹糸腺の予定細胞死における初期遺伝子の発現様式, 関本隆志; 岩見雅史; 桜井勝, 日本動物学会第73回大会, 27 Sep. 2002, 24 Sep. 2002, 27 Sep. 2002, Japanese
• カイコガ前部絹糸腺の予定細胞死における初期遺伝子の発現様式, 関本隆志; 岩見雅史; 桜井勝, 昆虫ワークショップ02, 12 Mar. 2002, 11 Mar. 2002, 13 Mar. 2002, Japanese
• Myc誘導性複製ストレスにおけるグアニン四重鎖構造は治療標的になり得るか, 関本隆志, 第46回 日本分子生物学会年会, 07 Dec. 2023, 06 Dec. 2023, 08 Dec. 2023, English, Japan, Domestic conference

Awards

• 2016

Research Projects

• Analysis of the regulatory mechanisms of Y-family DNA polymerases and its roles in precancerous lesions, SEKIMOTO Takayuki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Gunma University, Apr. 2010, Mar. 2012, In precancerous lesions, oncogenic signals induce cellular senescence, causing tumor suppression ; whereas they promote genomic instability, leading to tumor progression. We studied the role of DNA replicative stress in cyclin E over expressed U2OS human cells. Our data suggest that Y family Polymerases play a role in oncogene-induced DNA re-replication., Competitive research funding, 22790306

  対象リソースIRI
• YAMASHITA Takayuki; SEKIMOTO Takayuki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, Apr. 2019, Mar. 2022, Coinvestigator, 19K07772

  対象リソースIRI
• SEKIMOTO Takayuki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, Apr. 2018, Mar. 2021, Principal investigator, 18K07289

  対象リソースIRI
• Analysis of oncogene-induced replication stress response patyways, Sekimoto Takayuki; YAMASHITA Takayuki; ODA Tsukasa, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, Apr. 2015, Mar. 2018, Principal investigator, Growth of cancer cells relies on their tolerance of oncogene-induced replication stress (RS). Translesion synthesis (TLS) plays an essential role in cellular tolerance of various types of RS. However, limited information is available about the role of TLS polymerases in oncogene-induced RS. Polη, a Y-family TLS polymerase, promotes cellular tolerance of Myc-induced RS. Polη was recruited to Myc-induced RS sites, and Polη depletion enhanced the Myc-induced stalling of replication forks and the subsequent generation of double-strand breaks (DSBs). In the absence of Polη, Myc-induced DSB formation depended on MUS81-EME2, and concomitant depletion of MUS81-EME2 and Polη enhanced RS and cell death in a synergistic manner. Collectively, these results indicate that Polη facilitates fork progression during Myc-induced RS. Additionally, the present study highlights the possibility of a synthetic lethal interaction between Polη and MUS81-EME2 in cells experiencing Myc-induced RS., 15K06825

  対象リソースIRI
• The role of Y-family polymerase in aberrant DNA replication and genomic instability, Yamashita Takayuki; ODA Tsukasa; SEKIMOTO Takayuki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, Apr. 2015, Mar. 2018, Coinvestigator, Growth of neoplastic cells relies on their tolerance to oncogene-induced replication stress (RS). Translesion synthesis (TLS) plays a critical role in cellular tolerance to various types of RS by employing specialized polymerases. Here, we report that Polη, a Y-family TLS polymerase, promotes cellular tolerance to Myc-induced RS. Polη was recruited to Myc-induced RS sites, and Polη depletion enhanced the Myc-induced slowing and stalling of replication forks and the subsequent generation of double-strand breaks (DSBs). In the absence of Polη, Myc-induced DSB formation depended on MUS81-EME2 (the S-phase specific endonuclease complex), and concomitant depletion of MUS81-EME2 and Polη enhanced RS and cell death in a synergistic manner. Collectively, these results indicate that Polη alleviates the Myc-induced RS, and highlight the possibility of synthetic sick or lethal interaction between Polη and MUS81-EME2 in cells experiencing Myc-induced RS., 15K06826

  対象リソースIRI
• Analysis of replication stress responses caused by oncogenic signals, SEKIMOTO Takayuki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Gunma University, Apr. 2012, Mar. 2015, Principal investigator, DNA rereplication is a major type of oncogene-induced aberrant replication and plays an important role in genomic instability during tumor development. Rereplication causes DNA double strand breaks, resulting in copy number changes and genomic rearrangements. However, little is known on what DNA polymerases are involved in rereplication. Normal DNA replication is catalyzed by high-fidelity replicative polymerases, Pol ε and Pol δ. Y-family polymerases (Y-Pols) are the major group of low-fidelity polymerases whose main function is bypass of replication blocks at sites of DNA damages, i.e. translesion synthesis. We found that Y-Pols as well as replicative polymerases, participate in rereplication. The present findings have important mechanistic implications in genomic instability during tumorigenesis. Speculatively, the involvement of Y-Pols in rereplication may account for frequent occurrence of single nucleotide variations in conjunction with gene rearrangements in cancer genomes., 24700952

  対象リソースIRI
• Apr. 2012, Mar. 2014, Principal investigator

  対象リソースIRI
• Roles of replication stress for cellular senescence and genome instability in preneoplastic lesions, YAMASHITA Takayuki; ODA Tsukasa; SEKIMOTO Takayuki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, Apr. 2011, Mar. 2014, Coinvestigator, In preneoplastic lesions, oncogene-induced replication stress plays a dual role induction of cell senescence/apoptosis and tumor promotion through increased genomic instability. In these processes, oncogene-induced excessive activation of replication origins and consequent rereplication plays a major role. However, little is known about fork progression during rereplication. When rereplication was induced by depletion of geminin, a regulator of origin activation, Pol-eta was recruited to rereplication sites in human cell lines. Similar observations were obtained in cyclin E-induced rereplication in human tumor cells. Furthermore, Pol-eta knockdown suppressed rereplication induced by either geminin depletion or cyclin E expression. Together, our data suggest that Pol-eta participates in oncogene-induced rereplication. These findings provide important mechanistic insights into genomic instability during tumorigenesis., 23501257

  対象リソースIRI
• Apr. 2011, Apr. 2012, Principal investigator

  対象リソースIRI
• New understanding and therapeutics of hematopoietic diseases-from a viewpoint of regulatory mechanisms of replicative stress, YAMASHITA Takayuki; ODA Tsukasa; SEKIMOTO Takayuki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Gunma University, Apr. 2008, Mar. 2011, Coinvestigator, Fanconi anemia (FA) is genetically heterogeneous inherited bone marrow failure syndrome, characterized by cellular sensitivity to DNA crosslinkers and susceptibility to various types of malignant tumors including acute myeloid leukemia. Increasing evidence indicates that FA proteins protect against "replicative stress" induced by various genotoxic agents, cooperating with other machineries. Translesion DNA synthesis is one of such cellular protective mechanisms, using specialized DNA polymerases including Pol-eta and REV1.However, regulatory mechanism of these polymerases was largely unknown. We identified the molecular chaperone Hsp90 as an essential regulator of these polymerases. The present findings may lead to development of new therapy of hematopoietic diseases., 20591109

  対象リソースIRI

Social Contribution

Social Contribution

• まちなかキャンパス　(Target:　High school students, College students, Graduate students, Teachers, Guardians, General)
• 知の拠点【すぐわかアカデミア】研究紹介動画作成, 16 Oct. 2020, すぐにわかる肥満、糖尿病の現状とこれから, Others, No password, Internet　(Target:　Junior high school students, High school students, College students, Graduate students, Teachers, Guardians, General)

  添付ファイルのURL
• 群馬ちびっこ大学, 02 Aug. 2021, 22 Aug. 2021, 顕微鏡で見る生命の不思議, Others, No password, Internet　(Target:　Schoolchildren, Junior high school students, High school students, College students, Graduate students, Teachers, Guardians, General)

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• 研究所公式YouTubeにおける研究紹介動画の公開, 2021, 2021, No password, Internet　(Target:　Schoolchildren, Junior high school students, High school students, College students, Graduate students, Teachers, Guardians, General)

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• 研究所公式YouTubeにおける研究紹介動画の公開、管理, 2022, 2022, No password, Internet　(Target:　Schoolchildren, Junior high school students, High school students, College students, Graduate students, Teachers, Guardians, General)

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• 高校生のための最先端生命科学&重粒子線医学セミナー, 11 Mar. 2024, 11 Mar. 2024, Seminar　(Target:　Schoolchildren, High school students)
• 研究所公式YouTubeにおける研究紹介動画の公開、管理, 2023, 2023, No password, Internet　(Target:　Schoolchildren, Junior high school students, High school students, College students, Graduate students, Teachers, Guardians, General)

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