Researcher Profile and Settings

Researcher

• Name

  SHIBATA Takayuki

Profile and Settings

• Name

  Takayuki, Shibata

Foreign language

• Use in presentation

  English

• Use in publication

  English

Affiliation

• Gunma University, Graduate School of Health Science Domain of Laboratory Sciences, Associate Professor

Education

• 1999, 2002, Kyushu University, Graduate School of Pharmaceutical Sciences
• 1997, 1999, Kyushu University, Graduate School of Pharmaceutical Sciences
• 1993, 1997, Kyushu University, School of Pharmaceutical Sciences

Degree

• Ph.D

Association Memberships

• The Pharmaceutical Society of Japan, 1998
• The Japan Society for Analytical Chemistry, 2006
• Japanese Cancer Association, 2017

Research Experience

• May 2018, 9999, Gunma University, Graduate School of Health Sciences, Associate Professor, Associate prof lecturer lv
• 2007, 2018, Nagasaki University Graduate School of Biomedical Sciences, Pharmaceutical Sciences, Assistant Professor, Assistant prof lv
• 2006, 2007, Nagasaki University, Graduate School of Biomedical Sciences
• 2007, Nagasaki University, Graduate School of Biomedical Sciences
• 2002, 2006
• 2006, 2007, Research Associate,Pharmaceutical Sciences,Pharmaceutical Sciences,Graduate School of Biomedical Sciences,Nagasaki University
• 2007, Assistant Professor,Pharmaceutical Sciences,Pharmaceutical Sciences,Graduate School of Biomedical Sciences,Nagasaki University
• 2002, 2006, Researcher,The University of Sheffield

Research Activities

Research Areas

• Life science, Analytical chemistry
• Life sciences, Organic Chemistry
• Life sciences, Laboratory medicine

Research Interests

• Analytical Chemistry
• Organic Chemistry
• Clinical chemistry
• Nucleic Acids Chemistry

Published Papers

• A Facile Fluorometric Assay of Orotate Phosphoribosyltransferase Activity Using a Selective Fluorogenic Reaction for Orotic Acid, Takayuki Shibata, Tomohiro Narita, Yutaka Suto, Hasina Yasmin, Tsutomu Kabashima, 24 Feb. 2023, Sensors, 23, 5, 2507, 2507, Scientific journal

  DOI
• A Facile Method to Determine Prolidase Activity Using a Peptide-Specific Fluorometric Reaction, Tsutomu Kabashima, Nana Hamasaki, Keiko Tonooka, Takayuki Shibata, 01 Jan. 2023, Chemical and Pharmaceutical Bulletin, 71, 1, 15, 18, Scientific journal

  DOI
• Synthesis and Evaluation of Oligonucleotide-Containing 2-O-{[(4,5,8- Trimethylpsoralen)-4-ylmethoxy]ethylaminocarb-onyl}adenosine as Photo-crosslinkable Gene Targeting Tools, Yu Mikame, Yui Sakai, Ryo Tahara, Kinuka Doi, Tsuyoshi Yamamoto, Chikara Dohno, Takayuki Shibata, Asako Yamayoshi, 01 Oct. 2022, Chemical and Pharmaceutical Bulletin, 70, 10, 726, 730, Scientific journal
• A facile method for the quantification of urinary uracil concentration by a uracil-specific fluorescence derivatization reaction., Takayuki Shibata, Ryosuke Shimamura, Yuji Yamamoto, Hiroki Sakurai, Junya Fujita, Asako Yamayoshi, Toshimitsu Nemoto, Tsutomu Kabashima, 2021, Chemical and Pharmacertical Bulletin, Scientific journal
• Selective cross-linking behavior of oligodeoxyribonucleotides containing 2'-O-[N-(4,5',8-trimethylpsoralen-4'- ylmethylcarbamoyl)]adenosine to mutant H-ras DNA, Yamayoshi A, Higuchi M, Sakai Y, Kobori A, Yamamoto T, Shibata T, Murakami A, 2020, Nucleosides Nucleotides & Nucleic Acids, 39, 1-3, 119, 130, Scientific journal
• Hydrazide Derivatives of Luminol for Chemiluminescence-labelling of Macromolecules, Shibata T., Yoshimura H., Yamayoshi A., Tsuda N., Dragusha S., 2019, Chemical and Pharmaceutical Bulletin, 67, 8, 772, 774, Scientific journal

  DOI
• Simultaneous assay for protease activities of hepatitis C virus and human immunodeficiency virus based on fluorescence detection, Kabashima T, Tonooka K, TakadaM, Kai M, Shibata T, 2019, Scientific Reports, 9, 9150, Scientific journal

  DOI
• Facile and selective determination of dipeptides using 3-methylcatechol as a novel fluorogenic reagent., Yasmin H,Rahman MS,Shibata T, 2018, International Journal of Peptide Research and Therapeutics, 25, 2, 583, 589, Scientific journal

  DOI
• Fluorescence assay of dihydroorotate dehydrogenase that may become a cancer biomarker, Sheng Yin,Tsutomu Kabashima,Qinchang Zhu,Takayuki Shibata,Masaaki Kai, 2017, SCIENTIFIC REPORTS, 7, 40670, Scientific journal

  DOI
• Sensitive and Selective Determination of Orotic Acid in Biological Specimens Using a Novel Fluorogenic Reaction, Sheng Yin,Shpend Dragusha,Valon Ejupi,Takayuki Shibata,Tsutomu Kabashima,Masaaki Kai, Jul. 2015, JOURNAL OF FLUORESCENCE, 25, 4, 1005, 1011, Scientific journal

  DOI
• Fluorometric assay for phenotypic differentiation of drug-resistant HIV mutants, Qinchang Zhu,Zhiqiang Yu,Tsutomu Kabashima,Sheng Yin,Shpend Dragusha,Ahmed F. M. El-Mahdy,Valon Ejupi,Takayuki Shibata,Masaaki Kai, May 2015, SCIENTIFIC REPORTS, 5, 10323, Scientific journal

  DOI
• Spectrofluorometric Assays of Human Collagenase Activity Using Native Collagen and Acetyl-Peptide Substrates, Ejupi V,Dragusha S,Kabashima T,Zhu Q,El-Mahdy AF,Yin S,Shibata T,Kai M, 2015, Advances in Enzyme Research, 3, 19, 29, Scientific journal

  DOI
• Delivery of siRNA using siRNA/cationic vector complexes encapsulated in dendrimer-like polymeric DNAs, Ahmed F. M. El-Mahdy,Takayuki Shibata,Tsutomu Kabashima,Qinchang Zhu,Masaaki Kai, 2015, RSC ADVANCES, 5, 41, 32775, 32785, Scientific journal

  DOI
• Fluorescence detection of amino acids in the postcleavage conversions for manual sequencing of a peptide, Moses N. Wainaina,Takayuki Shibata,Chaivat Smanmoo,Tsutomu Kabashima,Masaaki Kai, Mar. 2008, ANALYTICAL BIOCHEMISTRY, 374, 2, 423, 425, Scientific journal

  DOI
• A selective fluorescence reaction for peptides and chromatographic analysis, Tsutomu Kabashima,Zhiqiang Yu,Chenhong Tang,Yoshiki Nakagawa,Kyosuke Okumura,Takayuki Shibata,Jianzhong Lu,Masaaki Kai, Mar. 2008, PEPTIDES, 29, 3, 356, 363, Scientific journal

  DOI
• Facile preparation of streptavidin-coated sephadex beads and their application to chemiluminescence detection of a target DNA, Ahmed F. M. El-Mahdy,Valon Ejupi,Takayuki Shibata,Tsutomu Kabashima,Jianzhong Lu,Masaaki Kai, Feb. 2015, MICROCHIMICA ACTA, 182, 3-4, 495, 503, Scientific journal

  DOI
• Sensitive and selective determination of peptides, PG and PGP, using a novel fluorogenic reagent 4-chlorobenzene-1,2-diol, Hasina Yasmin,Mohammed Shafikur Rahman,Takayuki Shibata,Tsutomu Kabashima,Masaaki Kai, Apr. 2015, CHEMICAL PAPERS, 69, 4, 504, 509, Scientific journal

  DOI
• Chemiluminescence-imaging detection of DNA on a solid-phase membrane by using a peroxidase-labeled macromolecular probe, Md Golam Azam,Mutsumi Yamasuji,Tomasz Krawczyk,Takayuki Shibata,Tsutomu Kabashima,Masaaki Kai, Jul. 2015, TALANTA, 139, 138, 142, Scientific journal

  DOI
• Diimine ligand as a novel chemiluminescence enhancer of luminol-containing compounds, Chaivat Smanmoo,Mutsumi Yamasuji,Tomoko Sagawa,Takayuki Shibata,Tsutomu Kabashima,De-Qi Yuan,Kahee Fujita,Masaaki Kai, Mar. 2009, TALANTA, 77, 5, 1761, 1766, Scientific journal

  DOI
• Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol-biotin chemiluminescent polymer, Huan Zhang,Takayuki Shibata,Tomasz Krawczyk,Tsutomu Kabashima,Jianzhong Lu,Myung K. Lee,Masaaki Kai, Aug. 2009, TALANTA, 79, 3, 700, 705, Scientific journal

  DOI
• A novel fluorescence reaction for N-terminal Ser-containing peptides and its application to assay caspase activity, Mohammed Shafikur Rahman,Tsutomu Kabashima,Hasina Yasmin,Takayuki Shibata,Masaaki Kai, Feb. 2013, ANALYTICAL BIOCHEMISTRY, 433, 2, 79, 85, Scientific journal

  DOI
• Selective and facile assay of human immunodeficiency virus protease activity by a novel fluorogenic reaction, Zhiqiang Yu,Tsutomu Kabashima,Chenhong Tang,Takayuki Shibata,Kaio Kitazato,Nobuyuki Kobayashi,Myung Koo Lee,Masaaki Kai, Feb. 2010, ANALYTICAL BIOCHEMISTRY, 397, 2, 197, 201, Scientific journal

  DOI
• A novel and specific fluorescence reaction for uracil, Takayuki Shibata,Shin-ya Kawasaki,Jun-ya Fujita,Tsutomu Kabashima,Masaaki Kai, Aug. 2010, ANALYTICA CHIMICA ACTA, 674, 2, 234, 238, Scientific journal

  DOI
• Insights into the DNA stabilizing contributions of a bicyclic cytosine analogue: crystal structures of DNA duplexes containing 7,8-dihydropyrido [2,3-d]pyrimidin-2-one, Ella Czarina Magat Juan,Satoru Shimizu,Xiao Ma,Taizo Kurose,Tsuyoshi Haraguchi,Fang Zhang,Masaru Tsunoda,Akihiro Ohkubo,Mitsuo Sekine,Takayuki Shibata,Christopher L. Millington,David M. Williams,Akio Takenaka, Oct. 2010, NUCLEIC ACIDS RESEARCH, 38, 19, 6737, 6745, Scientific journal

  DOI
• Alginic acid-based macromolecular chemiluminescent probe for universal protein assay on a solid-phase membrane, Tomasz Krawczyk,Midori Kondo,Md. Golam Azam,Huan Zhang,Takayuki Shibata,Masaaki Kai, 2010, ANALYST, 135, 11, 2894, 2900, Scientific journal

  DOI
• Novel synthesis of O-6-alkylguanine containing oligodeoxyribonucleotides as substrates for the human DNA repair protein, O-6-methylguanine DNA methyltransferase (MGMT), T Shibata,N Glynn,TBH McMurry,RS McElhinney,GP Margison,DM Williams, 2006, NUCLEIC ACIDS RESEARCH, 34, 6, 1884, 1891, Scientific journal

  DOI
• The syntheses and properties of tricyclic pyrrolo[2,3-d]pyrimidine analogues of S-6-methylthioguanine and O-6-methylguanine, AR Hornillo-Araujo,AJM Burrell,MK Aiertza,T Shibata,DM Hammond,D Edmont,H Adams,GP Margison,DM Williams, 2006, ORGANIC & BIOMOLECULAR CHEMISTRY, 4, 9, 1723, 1729, Scientific journal

  DOI
• A novel DNA damage recognition protein in Schizosaccharomyces pombe, Steven J. Pearson,Stephen Wharton,Amanda J. Watson,Ghazala Begum,Amna Butt,Nicola Glynn,David M. Williams,Takayuki Shibata,Mauro F. Santibanez-Koref,Geoffrey P. Margison, 2006, NUCLEIC ACIDS RESEARCH, 34, 8, 2347, 2354, Scientific journal

  DOI
• 7,8-dihydropyrido[2,3-d]pyrimidin-2-one; a bicyclic cytosine analogue capable of enhanced stabilisation of DNA duplexes, Takayuki Shibata,Niklaas J. Buurma,John A. Brazier,Peter Thompson,Ihtshamul Haq,David M. Williams, 2006, CHEMICAL COMMUNICATIONS, 33, 3516, 3518, Scientific journal

  DOI
• The synthesis and properties of tricyclic analogues of S-6-methylthioguanine and O-6-methylguanine, Ana R. Hornillo-Araujo,Adam J. M. Burrell,Miren K. Aiertza,Takayuki Shibata,David M. Hammond,Dolores Edmont,Harry Adams,Geoffrey P. Margison,David M. Williams, 2007, NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 26, 8-9, 1099, 1102, Scientific journal

  DOI
• Amino-functionalized DNA: the properties of C5-amino-alkyl substituted 2 '-deoxyuridines and their application in DNA triplex formation, JA Brazier,T Shibata,J Townsley,BF Taylor,E Frary,NH Williams,DM Williams, 2005, NUCLEIC ACIDS RESEARCH, 33, 4, 1362, 1371, Scientific journal

  DOI
• Inhibition of HIV-1 protease expression in T cells owing to DNA aptamer-mediated specific delivery of siRNA, Qinchang Zhu,Takayuki Shibata,Tsutomu Kabashima,Masaaki Kai, Oct. 2012, EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 56, 396, 399, Scientific journal

  DOI
• A manual sequence method of peptides and phosphopeptides using 4-(1 '-cyanoisoindolyl)phenylisothiocyanate, Takayuki Shibata,Moses N. Wainaina,Takayuki Miyoshi,Tsutomu Kabashima,Masaaki Kai, Jun. 2011, JOURNAL OF CHROMATOGRAPHY A, 1218, 24, 3757, 3762, Scientific journal

  DOI
• Chemiluminescence detection of telomere DNA in human cells on a membrane by using fluorescein-5-isothiocyanate-labeled primers, Mutsumi Yamasuji,Takayuki Shibata,Tsutomu Kabashima,Masaaki Kai, Jun. 2011, ANALYTICAL BIOCHEMISTRY, 413, 1, 50, 54, Scientific journal

  DOI
• Alkaline phosphatase-labeled macromolecular probe for sensitive chemiluminescence detection of proteins on a solid-phase membrane, Md. Golam Azam,Takayuki Shibata,Tsutomu Kabashima,Masaaki Kai, Sep. 2011, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 401, 4, 1211, 1217, Scientific journal

  DOI
• A Novel Fluorometric Method for the Selective Determination of Pro-Gly and Pro-Gly-Pro, Hasina Yasmin,Mohammed Shafikur Rahman,Takayuki Shibata,Tsutomu Kabashima,Masaaki Kai, Dec. 2014, INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, 20, 4, 441, 446, Scientific journal

  DOI
• Dendrimer-like polymeric DNAs as chemiluminescence probes for amplified detection of telomere DNA on a solid-phase membrane, Ahmed F. M. El-Mahdy,Takayuki Shibata,Tsutomu Kabashima,Masaaki Kai, 2014, CHEMICAL COMMUNICATIONS, 50, 7, 859, 861, Scientific journal

  DOI
• Amplified and selective assay of collagens by enzymatic and fluorescent reactions, Hasina Yasmin,Tsutomu Kabashima,Mohammed Shafikur Rahman,Takayuki Shibata,Masaaki Kai, May 2014, SCIENTIFIC REPORTS, 4, 4950, Scientific journal

  DOI
• Carbon Nanofiber-based Luminol-biotin Probe for Sensitive Chemiluminescence Detection of Protein, Stefan Baj,Tomasz Krawczyk,Natalia Pradel,Md Golam Azam,Takayuki Shibata,Shpend Dragusha,Krzysztof Skutil,Miroslawa Pawlyta,Masaaki Kai, Nov. 2014, ANALYTICAL SCIENCES, 30, 11, 1051, 1056, Scientific journal

  DOI
• Selective, sensitive and fluorometric determination of urinary cytosine with 4-trifluoromethylbenzamidoxime and N,N-dimethylformamide, Shpend Dragusha,Takayuki Shibata,Sheng Yin,Jun-ya Fujita,Tsutomu Kabashima,Masaaki Kai, Feb. 2014, CLINICA CHIMICA ACTA, 429, 123, 128, Scientific journal

  DOI
• 慢性閉塞性肺疾患の診断を指向したペプチドマーカーの高感度検出法, 柴田 孝之,宗 初枝, 2018, 38, 1, 89, 92, Scientific journal
• ペプチド配列特異的な蛍光反応を利用した慢性閉塞性肺疾患のスクリーニング検査法, 柴田 孝之, 2017, 37, 7, 667, 670, Scientific journal
• 慢性閉塞性肺疾患の早期検査法の開発, 柴田 孝之, 2017, 37, 4, 364, 367, Scientific journal
• Selective FL Quenching or Enhancing of Diimine Ligands by Guanine, Smanmoo\, Srung,Kawasaki\, Shinya,Tangboriboonrat\, Pramuan,Shibata\, Takayuki,Kabashima\, Tsutomu,Kai\, Masaaki, Sep. 2013, JOURNAL OF FLUORESCENCE, 23, 5, 853, 857, Scientific journal

  DOI
• A novel fluorescence reaction for N-terminal Ser-containing peptides and its application to assay caspase activity, Rahman\, Mohammed Shafikur,Kabashima\, Tsutomu,Yasmin\, Hasina,Shibata\, Takayuki,Kai\, Masaaki, 2013, ANALYTICAL BIOCHEMISTRY, 433, 2, 79, 85

  DOI
• Sensitive chemiluminescence detection of prion protein on a membrane by using a peroxidase-labeled dextran probe., Azam G,Shibata T,Kabashima T,Kai M, 2011, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 27, 7, 715
• Aptamer-mediated chemiluminescence detection of prion protein on a membrane using trimethoxyphenylglyoxal., Hossain MT,Shibata T,Kabashima T,Kai M, 2010, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 26, 6, 645, 647, Scientific journal
• Facile assay of telomerase activity utilizing a DNA-detectable chemiluminogenic reagent., Tonooka K,Kabashima T,Shibata T,Tang C,Yu Z,Kai M, Apr. 2008, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 24, 4, 471, 475, Scientific journal
• Crystal structures of DNA duplexes stabilized by bicyclic-C residues., Haraguchi T,Shimizu S,Ma X,Kurose T,Juan EC,Ohkubo A,Sekine M,Shibata T,Millington CL,Williams DM,Takenaka A, 2008, Nucleic acids symposium series (2004), 52, 127, 128, International conference proceedings

  DOI
• X-Ray analyses of DNA duplexes stabilized by bicyclic-C residues., Ma X,Kurose T,Juan EC,Shibata T,Williams DM,Takenaka A, 2006, Nucleic acids symposium series (2004), 50, 213, 214, International conference proceedings

  DOI
• Novel post DNA synthesis chemistry for preparing oligonucleotides containing O6-modified purines., Shibata T,Williams DM, 2005, Nucleic acids symposium series (2004), 49, 23, 24, International conference proceedings

  DOI
• Hydrazide Derivatives of Luminol for Chemiluminescence-Labelling of Macromolecules, Shibata, Takayuki;Yoshimura, Hiroki;Yamayoshi, Asako;Tsuda, Nobuaki;Dragusha, Shpend, 2019, CHEMICAL & PHARMACEUTICAL BULLETIN, 67, 8, 772, 774
• Chemistry and Clinical Study in Concert, Shibata Takayuki, 2019, The Kitakanto Medical Journal, 69, 2, 161, 162
• Hydrazide Derivatives of Luminol for Chemiluminescence-Labelling of Macromolecules., Shibata Takayuki;Yoshimura Hiroki;Yamayoshi Asako;Tsuda Nobuaki;Dragusha Shpend, 17 May 2019, Chemical & pharmaceutical bulletin, 67, 8

  DOI
• Selective cross-linking behavior of oligodeoxyribonucleotides containing 2'-O-[N-(4,5',8-trimethylpsoralen-4'-ylmethylcarbamoyl)]adenosine to mutant H-ras DNA., Yamayoshi Asako;Higuchi Maiko;Sakai Yui;Kobori Akio;Yamamoto Tsuyoshi;Shibata Takayuki;Murakami Akira, 24 Oct. 2019, Nucleosides, nucleotides & nucleic acids, 39, 1-3

  DOI
• 置換基を有する弱酸特異的蛍光ラベル化剤の骨格の探索, ;, Aug. 2020, 70, 3, 293
• Simultaneous assay for protease activities of hepatitis C virus and human immunodeficiency virus based on fluorescence detection, Kabashima, Tsutomu;Tonooka, Keiko;Takada, Makoto;Kai, Masaaki;Shibata, Takayuki, 2019, SCIENTIFIC REPORTS, 9

  DOI
• 中性領域でのみ発光する新規蛍光色素の合成と蛍光特性の評価, ;;, Aug. 2020, 70, 3, 292
• 置換基を有する弱酸特異的蛍光ラベル化剤の骨格の探索, ;, Aug. 2020, 70, 3, 293
• ジヒドロピリミジンデヒドロゲナーゼの逆反応追跡による活性測定, ;;;, Aug. 2020, 70, 3, 293
• 中性選択的蛍光色素における蛍光波長の長波長化とpH範囲選択性の向上, ;;;, Aug. 2021, 71, 3, 252
• ジヒドロピリミジンデヒドロゲナーゼの活性測定を可能にする基質の合成, ;;;, Aug. 2022, 72, 3, 320
• A Facile Method for the Quantification of Urinary Uracil Concentration by a Uracil-Specific Fluorescence Derivatization Reaction., Shibata Takayuki;Shimamura Ryosuke;Yamamoto Yuji;Sakurai Hiroki;Fujita Junya;Yamayoshi Asako;Nemoto Toshimitsu;Kabashima Tsutomu, Chemical & pharmaceutical bulletin, 69, 8

  DOI
• Synthesis and Evaluation of Oligonucleotide-Containing 2'-O-{[(4,5',8-Trimethylpsoralen)-4'-ylmethoxy]ethylaminocarb-onyl}adenosine as Photo-crosslinkable Gene Targeting Tools., Mikame Yu;Sakai Yui;Tahara Ryo;Doi Kinuka;Yamamoto Tsuyoshi;Dohno Chikara;Shibata Takayuki;Yamayoshi Asako, Jul. 2022, Chemical & pharmaceutical bulletin, 70, 10

  DOI
• A Facile Method to Determine Prolidase Activity Using a Peptide-Specific Fluorometric Reaction., Kabashima Tsutomu;Hamasaki Nana;Tonooka Keiko;Shibata Takayuki, Chemical & pharmaceutical bulletin, 71, 1

  DOI
• A Facile Fluorometric Assay of Orotate Phosphoribosyltransferase Activity Using a Selective Fluorogenic Reaction for Orotic Acid., Shibata Takayuki;Narita Tomohiro;Suto Yutaka;Yasmin Hasina;Kabashima Tsutomu, Feb. 2023, Sensors (Basel, Switzerland), 23, 5

  DOI
• Selective cross-linking behavior of oligodeoxyribonucleotides containing 2'-O-[N-(4,5',8-trimethylpsoralen-4'-ylmethylcarbamoyl)]adenosine to mutant H-ras DNA, Yamayoshi, Asako;Higuchi, Maiko;Sakai, Yui;Kobori, Akio;Yamamoto, Tsuyoshi;Shibata, Takayuki;Murakami, Akira, 2020, NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 39, 1-3, 119, 130

  DOI
• A Facile Method for the Quantification of Urinary Uracil Concentration by a Uracil-Specific Fluorescence Derivatization Reaction, Shibata, Takayuki;Shimamura, Ryosuke;Yamamoto, Yuji;Sakurai, Hiroki;Fujita, Junya;Yamayoshi, Asako;Ncmoto, Toshimitsu;Kabashima, Tsutomu, 2021, CHEMICAL & PHARMACEUTICAL BULLETIN, 69, 8, 768, 772

  DOI
• Synthesis and Evaluation of Oligonucleotide-Containing 2′-O-{[(4,5′,8-Trimethylpsoralen)-4′-ylmethoxy]ethylaminocarb-onyl}adenosine as Photo-crosslinkable Gene Targeting Tools, Mikame, Yu;Sakai, Yui;Tahara, Ryo;Doi, Kinuka;Yamamoto, Tsuyoshi;Dohno, Chikara;Shibata, Takayuki;Yamayoshi, Asako, 2022, CHEMICAL & PHARMACEUTICAL BULLETIN, 70, 10, 726, 730
• A Facile Method to Determine Prolidase Activity Using a Peptide-Specific Fluorometric Reaction, Kabashima, Tsutomu;Hamasaki, Nana;Tonooka, Keiko;Shibata, Takayuki, 2023, CHEMICAL & PHARMACEUTICAL BULLETIN, 71, 1, 15, 18
• A Facile Fluorometric Assay of Orotate Phosphoribosyltransferase Activity Using a Selective Fluorogenic Reaction for Orotic Acid, Shibata, Takayuki;Narita, Tomohiro;Suto, Yutaka;Yasmin, Hasina;Kabashima, Tsutomu, 2023, SENSORS, 23, 5

  DOI
• A Facile Method for the Quantification of Urinary Uracil Concentration by a Uracil-Specific Fluorescence Derivatization Reaction, Shibata Takayuki;Shimamura Ryosuke;Yamamoto Yuji;Sakurai Hiroki;Fujita Junya;Yamayoshi Asako;Nemoto Toshimitsu;Kabashima Tsutomu, Aug. 2021, Chemical and Pharmaceutical Bulletin, 69, 8, 768, 772

  DOI
• 中性選択的蛍光色素における蛍光波長の長波長化とpH範囲選択性の向上, ;;;, Aug. 2021, 71, 3, 252

Presentations

• pH 範囲選択的な新規蛍光色素の開発と細胞染色への展開, 相澤 よしの, Islam Md Ashraful, 中本 智也, 多胡憲治, 須藤豊, 柴田孝之, 日本薬学会第145年会, 29 Mar. 2025, 27 Mar. 2025, 29 Mar. 2025, Japanese, Domestic conference
• がん簡易診断を指向した脂肪族高級アルデヒドの高感度検出法, 鎌形樹己, 多胡憲治, 柴田孝之, 日本薬学会第145年会, 27 Mar. 2025, 27 Mar. 2025, 29 Mar. 2025, Japanese, Domestic conference
• 吸光光度法を用いたジヒドロピリミジンデヒドロゲナーゼ活性測定法の開発, 佐藤寧香, 工藤由瑛, 柴田孝之, 日本薬学会第145年会, 27 Mar. 2025, 27 Mar. 2025, 29 Mar. 2025, Japanese, Domestic conference
• pH 選択的蛍光色素の置換基の位置と蛍光特性の関係性, 中本智也, Islam Md Ashraful, 直井美徳, 相 澤よしの, 多胡憲治, 須藤豊, 柴田孝之, 日本薬学会第145年会, 27 Mar. 2025, 27 Mar. 2025, 29 Mar. 2025, Japanese, Domestic conference
• Synthesis and characterization of a novel fluorescent dye with neutral pH-specific emission, Md Ashraful Islam, Minori Naoi, Yoshino Aizawa, Tomoya Nakamoto, Kenji Tago, Hasina Yasmin, Yutaka Suto and Takayuki Shibata, Gunma International Symposium 2025 on Biomolecular Science and Membrane Application, 23 Jan. 2025, 23 Jan. 2025, 23 Jan. 2025, English, Gunma, International conference
• pH範囲選択的な新規蛍光色素の開発と蛍光特性の評価, 相澤 よしの、中本智也、嘉手苅究、須藤豊、柴田孝之, 日本分析化学会第73年会, 12 Sep. 2024, 11 Sep. 2024, 13 Sep. 2024, Japanese, Japan, Domestic conference
• 微小環境のpHを測定できる蛍光色素, 柴田孝之, イノベーション・ジャパン2024, 23 Aug. 2024, 22 Aug. 2024, 23 Aug. 2024, Japanese, Japan, International conference
• ジヒドロピリミジンデヒドロゲナーゼの活性測定を可能にする基質の合成, 鈴木里彩、須藤豊、輿石一郎、柴田孝之, 第69回北関東医学会総会, 29 Sep. 2022, 29 Sep. 2022, 30 Sep. 2022, Japanese, Japan, Domestic conference
• A novel fluorescence dye that emits light under a certain pH region., Takayuki Shibata, Innovation japan 2020, 28 Sep. 2020, 30 Nov. 2020, International conference
• 中性領域でのみ発光する新規蛍光色素の合成と蛍光特性の評価, 直井美徳、須藤豊、柴田孝之, 第67回北関東医学会総会, 24 Sep. 2020, 24 Sep. 2020, 25 Sep. 2020, Domestic conference
• 置換基を有する弱酸特異的蛍光ラベル化剤の骨格の探索, 山浦裕紀、柴田孝之, 第67回北関東医学会総会, 24 Sep. 2020, 24 Sep. 2020, 25 Sep. 2020, Domestic conference
• ジヒドロピリミジンデヒドロゲナーゼの逆反応追跡による活性測定, 山本裕二、輿石一郎、時田佳治、柴田孝之, 第67回北関東医学会総会, 24 Sep. 2020, 24 Sep. 2020, 25 Sep. 2020, Domestic conference
• 特定のpH領域で蛍光を発する新規蛍光色素, 柴田孝之, BioJapan2019, 11 Oct. 2019, 09 Oct. 2019, 11 Oct. 2019, International conference
• 弱酸性条件下のみで発光する 新規蛍光物質, 柴田孝之, JST新技術説明会, 27 Jun. 2019, Domestic conference
• DNA methyltransferase活性測定用の新規FRET基質の開発, 椛島　力、安達　杏奈、甲斐　雅亮、柴田　孝之, 日本薬学会第138年会, 25 Mar. 2018, 28 Mar. 2018, Domestic conference
• 4-Trifluorobenzamidoxime as a specific fluorogenic reagent for cytosine, 日本薬学会第132年会, 2012, Japanese, Domestic conference
• ウラシル特異的な蛍光反応によるDPD欠損の簡易診断, 第26回 国際バイオEXPO, 2011
• Sensitive Fluorescence-Detection Method of Amino Acids for Protein Sequencing, International Conference in Structural Biology, 2007
• Application of DNA-detectable chemiluminogenic reagent (TMPG) to telomerase assay, Pure and Applied Chemistry International Conference 2008, 2008
• Preparation of super-sensitive luminescent probe for detection genes and proteins on microchips, Pure and Applied Chemistry International Conference 2008, 2008
• ピリミジン塩基を識別できる新規蛍光反応の開発と反応機構の解明, 日本分析化学会第57年会, 2008
• A fluorometric HPLC method for the assay of HIV protease activity, 日本分析化学会第57年会, 2008
• Selection of DNA aptamer against cytochrome P450, 第25回日本薬学会九州支部大会, 2008
• Alginic acid-based macromolecular probe for chemiluminescent detection of protein, The second Asia symposium on pharmaceutical sciences in Nagasaki, 2009
• Novel Fluorometric assay for identification of mutant hiv protease with HPLC, The second Asia symposium on pharmaceutical sciences in Nagasaki, 2009
• Development of sequence determination method for peptides with fluorogenic Edman reagent, 4-(1’-cyanoisoindolyl)phenylisothiocyanate, The second Asia symposium on pharmaceutical sciences in Nagasaki, 2009
• Development of simple method for DNA aptamer against protein, The second Asia symposium on pharmaceutical sciences in Nagasaki, 2009
• NEW TOOLS FOR THE RESEARCH ON HIV AND PRION DISEASES, The second Asia symposium on pharmaceutical sciences in Nagasaki, 2009
• Polysaccaride-based chemiluminescent probe for proteins detection on a membrane, 日本薬学会第129年会, 2009
• Sequential analysis of amino acids in peptides with fluorogenic Edman reagent, 4-(1’-cyanoisoindolyl) phenylisothiocyanate, 日本薬学会第129年会, 2009
• プリオンタンパク質の物理化学的性質に対する超音波刺激の影響, 第二回 長崎プリオン研究会, 2009
• 修飾塩基を持つsiRNAの設計と干渉効果の評価 (1), 第26回日本薬学会九州支部大会, 2009
• Evaluation on siRNA-induced inhibition of HIV-1 protease in mammalian cells, 日本薬学会第130年会, 2010
• Dextran-based enzymatic probes for sensitive imaging of chemiluminescence, 日本薬学会第130年会, 2010
• 3-メチルベンズアミドオキシムを用いた尿中ウラシル定量法およびジヒドロピリミジン脱水素酵素欠損症診断への応用, 日本分析化学会第60年会, 2011
• Inhibition of HIV-1 Protease Expression in T cells Using CD4 aptamer-siRNA Chimeras, 第28回日本薬学会九州支部大会, 2011
• Sensitive Fluorescence-Detection Method of Amino Acids for Protein Sequencing, 2007
• Alginic acid-based macromolecular probe for chemiluminescent detection of protein, 2009
• Inhibition of HIV-1 Protease Expression in T cells Using CD4 aptamer-siRNA Chimeras, 2011

Works

• 2011, 9999
• 2010, 9999
• 2010
• 2011, 2012

Industrial Property Rights

• Patent right, Takayuki Shibata, 特願2024-140075, 21 Aug. 2024, Gunma University, Yes, No
• Patent right, Takayuki Shibata, 特願2024-046883, 22 Mar. 2024, Gunma University, Yes, No
• Patent right, 特願2020-057546, 27 Mar. 2020
• Patent right, 特願2016-068416, 30 Mar. 2016, 特開2016-193897, 17 Nov. 2016
• Patent right, 特願2016-014936, 28 Jan. 2016, 特開2017-133978, 03 Aug. 2017
• Patent right, 特願2011-187969, 30 Aug. 2011, 特開2012-194170, 11 Oct. 2012, 特許第5817980号, 09 Oct. 2015
• Patent right, 特願2011-187969, 30 Aug. 2011, 特開2012-194170, 11 Oct. 2012
• Patent right, 特願2011-045488, 02 Mar. 2011, 特開2011-200226, 13 Oct. 2011, 特許第5818059号, 09 Oct. 2015
• Patent right, 特願2011-045485, 02 Mar. 2011, 特開2012-179027, 20 Sep. 2012
• Patent right, 特願2011-045488, 02 Mar. 2011, 特開2011-200226, 13 Oct. 2011
• Patent right, JP2011054636, 01 Mar. 2011, WO2011-108544, 09 Sep. 2011
• Patent right, 特願2012-503193, 01 Mar. 2011, 特許第5586034号, 01 Aug. 2014
• Patent right, URACIL-SPECIFIC FLUORESCENCE DETECTION REACTION AND METHOD FOR EXAMINING DIHYDROPYRIMIDINE DEHYDROGENASE DEFICIENCY

Research Projects

• 2020, Principal investigator
• 2019, Principal investigator
• Apr. 2017, Mar. 2018, Principal investigator, Competitive research funding
• Apr. 2015, Mar. 2017, Principal investigator, Competitive research funding
• Apr. 2012, Mar. 2013, Principal investigator, Competitive research funding
• Apr. 2011, Mar. 2012, Principal investigator, Competitive research funding
• Apr. 2011, Mar. 2014, Principal investigator, Competitive research funding
• Apr. 2010, Mar. 2011, Principal investigator, Competitive research funding
• Apr. 2008, Mar. 2009, Principal investigator, Competitive research funding
• A new generation of Edman degradation that enables amino acid sequencing of phosphorylated peptides., Grant-in-Aid for Promotion of Private Scientific Research, 2010, Competitive research funding
• Highthrouput diagnosis of dihydropyrimidine dehydrogenase deficiency, 0113 (Japanese Only), 2010, Competitive research funding

Social Contribution

Social Contribution

• 群⾺県臨床検査技師会　第4・５回臨床化学研究班例会, 30 Aug. 2022, 06 Sep. 2022
• ぐんまちびっこ大学, 02 Aug. 2022, 31 Mar. 2023, Internet　(Target:　Schoolchildren, Junior high school students, High school students, Guardians)
• 群馬ちびっこ大学, 02 Aug. 2021, 31 Mar. 2022, YouTube, Internet　(Target:　Schoolchildren, Junior high school students, High school students, Guardians)

  添付ファイルのURL
• イノベーション・ジャパン2020参加, 28 Sep. 2020, 30 Nov. 2020, Seminar

Academic Contribution

• 群馬保健学研究　副編集委員長, Peer review etc, 01 Apr. 2020, 9999