Researcher Profile and Settings

Researcher

• Name

  Ishii Nozomi

Affiliation

• Department of Materials and Bioscience, Assistant Professor

Profile and Settings

• Name

  Ishii, Nozomi

ホームページ

• URI

  https://matsuo.chem-bio.st.gunma-u.ac.jp/

Affiliation

• Affiliation

  Graduate School of Science and Technology

Education

• Apr. 2015, Mar. 2018, Gunma University, Graduate School of Science and Technology
• Apr. 2013, Mar. 2015, Gunma University, Graduate School of Science and Technology
• Apr. 2009, Mar. 2013, Gunma University

Association Memberships

• The Chemical Society of Japan, 01 May 2021
• Mar. 2020, 9999
• The New Zealand Institute of Chemistry (NZIC), Feb. 2019, 9999
• The Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA), Mar. 2014, 9999
• The Japanese Society of Carbohydrate Research (JSCR), May 2013, 9999

Research Experience

• Sep. 2020, 9999, Gunma University, Division of Molecular Science, Assistant Professor, Assistant prof lv
• Apr. 2018, Aug. 2020, University of Canterbury, Biomolecular Interaction Centre (BIC), Researcher postdoc lv

Research Activities

Research Areas

• Life sciences, Bioorganic chemistry

Research Interests

• Glycobiology
• Glycochemistry

Published Papers

• Synthesis of a heptasaccharide N-glycan comprising two mannose-6-phosphate residues, Nozomi Ishii, Andrea Mascherpa, Antony J. Fairbanks, Jan. 2025, Carbohydr. Res., 547, 109327

  DOI
• Structural basis of sugar recognition by SCFFBS2 ubiquitin ligase involved in NGLY1 deficiency., Tadashi Satoh, Maho Yagi-Utsumi, Nozomi Ishii, Tsunehiro Mizushima, Hirokazu Yagi, Ryuichi Kato, Yuriko Tachida, Hiroaki Tateno, Ichiro Matsuo, Koichi Kato, Tadashi Suzuki, Yukiko Yoshida, Sep. 2024, FEBS letters

  DOI
• Sugar-mediated non-canonical ubiquitination impairs Nrf1/NFE2L1 activation, Yukiko Yoshida, Tsuyoshi Takahashi, Nozomi Ishii, Ichiro Matsuo, Satoshi Takahashi, Haruka Inoue, Akinori Endo, Hikaru Tsuchiya, Meari Okada, Chikara Ando, Takehiro Suzuki, Naoshi Dohmae, Yasushi Saeki, Keiji Tanaka, Tadashi Suzuki, Aug. 2024, Molecular Cell, 84, 16, 3115, 3127, Scientific journal

  DOI
• Synthesis of a fluorescent probe for measuring the activity of endo-β-N-acetylglucosaminidases recognizing hybrid-type N-glycans., Ishii Nozomi;Inoue Shusei;Sano Kanae;Takahashi Satoshi;Matsuo Ichiro, Feb. 2024, Bioorganic & medicinal chemistry, 100, 117612, Scientific journal

  DOI
• Rapid preparation of a glycan oxazoline and a homogeneously glycosylated antibody with an enzyme-immobilized monolithic column., Muto Hiroshi; Ishii Nozomi; Iwamoto Mitsuhiro; Matsuo Ichiro, Feb. 2024, Carbohydrate Research, 536, 109024 (pp.1-9), Scientific journal

  DOI
• A silyl porphyrin derivative conjugated with 6-deoxy-6-sulfo-α-d-glucopyranose functions as an efficient photosensitizer for photodynamic therapy., Horiuchi Hiroaki; Nishikawa Kota; Ishii Nozomi; Kano Koki; Shinada Shunsuke; Osawa Nene; Horikoshi Aoi; Yoshihara Toshitada; Sugawara Fumio; Sakaguchi Kengo; Okustu Tetsuo; Katsura Shinji; Matsuo Ichiro; Oshige Masahiko, Feb. 2024, Photodiagnosis and Photodynamic Therapy, 45, 103898 (pp.1-7), Scientific journal

  DOI
• Convergent synthesis of oligomannose-type glycans via step-economical construction of branch structures., Sano Kanae; Ishii Nozomi; Takahashi Satoshi; Takeda Yoichi; Matsuo Ichiro, Mar. 2023, Carbohydrate Research, 525, 108764 (pp.1-6), Scientific journal

  DOI
• Protecting-group-free glycosylation of phosphatidic acid in aqueous media., Kano Koki; Ishii Nozomi; Miyagawa Atsushi; Takeda Hiroaki; Hirabayashi Yoshio; Kamiguchi Hiroyuki; Greimel Peter; Matsuo Ichiro, Feb. 2023, Organic & Biomolecular Chemistry, 21, 10, 2138, 2142, Scientific journal

  DOI
• A fluorogenic probe for core-fucosylated glycan-preferred ENGase., Ishii Nozomi; Muto Hiroshi; Nagata Mitsuo; Sano Kanae; Sato Itsuki; Iino Kenta; Matsuzaki Yuji; Katoh Toshihiko; Yamamoto Kenji; Matsuo Ichiro, Jan. 2023, Carbohydrate Research, 523, 108724 (pp.1-11), Scientific journal

  DOI
• Lysosomal Targeting of β-Cyclodextrin, Mascherpa Andrea; Ishii Nozomii; Tayagui Ayelen; Liu Jiang; Sollogoub Matthieu; Fairbanks Antony J., Jan. 2023, Chemistry A European Journal, 29, 4, e202203252 (pp.1-7), Scientific journal

  DOI
• Luminescence detection of peptide:N-glycanase activity using engineered split inteins., Takahashi Tsuyoshi; Uchibayashi Tatsuya; Ishii Nozomi; Matsuo Ichiro; Yoshida Yukiko; Suzuki Tadashi, Nov. 2022, Chemical Communications (Cambridge, England), 58, 95, 13282, 13285, Scientific journal

  DOI
• Occurrence of free N-glycans with a single GlcNAc at the reducing termini in animal sera., Huang Chengcheng; Seino Junichi; Fujihira Haruhiko; Sato Keiko; Fujinawa Reiko; Sumer-Bayraktar Zeynep; Ishii Nozomi; Matsuo Ichiro; Nakaya Shuichi; Suzuki Tadashi, Mar. 2022, Glycobiology, 32, 4, 314, 332, Scientific journal

  DOI
• Synthesis of 3-octadecanoxypropyl 6-deoxy-6-sulfo-α-D-glucopyranoside (ODSG) as a lipase-resistant SQAP derivative., Oshige Masahiko; Kano Koki; Shinada Shunsuke; Kawaguchi Akifumi; Uchida Takato; Ishii Nozomi; Horiuchi Hiroaki; Sugawara Fumio; Sakaguchi Kengo; Matsuo Ichiro; Katsura Shinji, Nov. 2021, Bioorganic & Medicinal Chemistry Letters, 52, 128391 (pp.1-3), Scientific journal

  DOI
• Stereocontrolled Synthesis of Lyso-phosphatidyl β-D-Glucoside, Kano Koki; Ishii Nozomi; Hirabayashi Yoshio; Kamiguchi Hiroki; Greimel Peter; Matsuo Ichiro, Jul. 2021, ChemistrySelect, 6, 6811, 6815, Scientific journal

  DOI
• Efficient synthesis of alpha(1,2)-linked oligomannoside derivatives through one-pot glycosylation, Sano Kanae; Ishii Nozomi; Kosugi Misa; Kuroiwa Ayumi; Matsuo Ichiro, Aug. 2020, Carbohydrate Research, 494, 108072 (pp.1-7), Scientific journal

  DOI
• Evaluation of Endo-beta-N-Acetylglucosaminidase Activity Based on a FRET-Quenching System, Nozomi Ishii, May 2020, TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY, 32, 187, E105, E108, Scientific journal

  DOI
• Fluorogenic probe for measuring high-mannose type glycan-specific endo-beta-N-acetylglucosaminidase H activity, Ishii Nozomi; Sano Kanae; Matsuo Ichiro, Jul. 2019, Bioorganic & Medicinal Chemistry Letters, 29, 13, 1643, 1646, Scientific journal

  DOI
• Fluorescence Quenching-based Assay for Measuring Golgi endo-alpha-Mannosidase, Sano Kanae; Kuribara Taiki; Ishii Nozomi; Kuroiwa Ayumi; Yoshihara Toshitada; Tobita Seiji; Totani Kiichiro; Matsuo Ichiro, Jun. 2019, Chemistry - An Asian Journal, 14, 11, 1965, 1969, Scientific journal

  DOI
• A bioactive mammalian disaccharide associated with autoimmunity activates STING-TBK1-dependent immune response, Fermaintt Charles S.; Sano Kanae; Liu Zhida; Ishii Nozomi; Seino Junichi; Dobbs Nicole; Suzuki Tadashi; Fu Yang-Xin; Lehrman Mark A.; Matsuo Ichiro; Yan Nan, May 2019, Nature Communications, 10, Scientific journal

  DOI
• A New Fluorogenic Probe for the Detection of endo-beta-N-Acetylglucosaminidase, Ishii Nozomi; Sunaga Chie; Sano Kanae; Huang Chengcheng; Iino Kenta; Matsuzaki Yuji; Suzuki Tadashi; Matsuo Ichiro, Apr. 2018, ChemBioChem, 19, 7, 660, 663, Scientific journal

  DOI
• A novel glucuronoyl esterase from Aspergillus fumigatus-the role of conserved Lys residue in the preference for 4-O-methyl glucuronoyl esters, Huynh Hung-Hiep; Ishii Nozomi; Matsuo Ichiro; Arioka Manabu, Mar. 2018, Applied Microbiology and Biotechnology, 102, 5, 2191, 2201, Scientific journal

  DOI
• Specificity of Donor Structures for endo-beta-N-Acetylglucosaminidase-Catalyzed Transglycosylation Reactions, Ishii Nozomi; Ogiwara Ken; Sano Kanae; Kumada Jyunichi; Yamamoto Kenji; Matsuzaki Yuji; Matsuo Ichiro, Jan. 2018, ChemBioChem, 19, 2, 136, 141, Scientific journal

  DOI
• Preferential binding of E-coli with type 1 fimbria to D-mannobiose with the Man alpha 1 -> 2Man structure, Ajisaka Katsumi; Yuki Kaoru; Sato Kaori; Ishii Nozomi; Matsuo Ichiro; Kuji Ryo; Miyazaki Tatsuo; Furukawa Kiyoshi, Jan. 2016, Bioscience, Biotechnology, and Biochemistry, 80, 1, 128, 134, Scientific journal

  DOI

MISC

• Chemical Synthesis of Phosphatidylglucoside, Koki Kano, Nozomi Ishii, Peter Greimel, Ichiro Matsuo, 01 Jan. 2023, Glycolipids: Methods and Protocols, 73, 78, Technical report
• α-Selective Dehydrative Glycosylation of 2-Deoxy- and 2,6-Dideoxy Sugars Using Cyclic Geminal Dihalides, Nozomi Ishii, 25 May 2022, Trends in Glycoscience and Glycotechnology, 34, 199, E61, J61, Book review
• 糖ヌクレオチドの新規ワンポット簡便合成 糖鎖工学への展開を目指して, 石井 希実, 01 Sep. 2021, 76, 9, 65, 66
• DMC-mediated Glycosylation of Unprotected Sugars in Water, Nozomi Ishii, 25 May 2021, Trends in Glycoscience and Glycotechnology, 33, 193, 77, 78, Book review
• 【糖鎖がついにわかる!狙える!診断薬・治療薬イノベーションを導く生命鎖】均一な糖タンパク質をつくる　アスパラギン結合型糖鎖の化学合成, 松尾 一郎;石井 希実;宇津井 隆志;佐野 加苗, Jun. 2017, 35, 9, 1434
• Analysis of substrate specificity for endo-M and its mutants using synthetic glycans, Nozomi Ishii; Shogo Iwamoto; Jyunichi Kumada; Yuji Matsuzaki; Ichiro Matsuo, Nov. 2014, GLYCOBIOLOGY, 24, 11, 1118, 1118, Summary international conference
• Convergent synthesis of biantennary complex type nonasaccharide containing LacdiNAc structure based on the regioselective glycosylation and inversion reactions, Shinpei Miyazawa; Nozomi Ishii; Takashi Utsui; Masaki Hamada; Ichiro Matsuo, Nov. 2014, GLYCOBIOLOGY, 24, 11, 1112, 1113, Summary international conference

Books etc

• Glycolipids, Methods in molecular biology (Clifton, N.J.), Contributor, Kano Koki;Ishii Nozomi;Greimel Peter;Matsuo Ichiro, Kano Koki;Ishii Nozomi;Greimel Peter;Matsuo Ichiro, Chemical Synthesis of Phosphatidylglucoside, Humana New York, Jan. 2023, 73-78, Textbook, ISBN: 978-1-0716-2909-3

  DOI

Presentations

• 糖鎖分子プローブによる糖加水分解酵素の活性検出, 石井 希実、飯野 健太、松尾 一郎, 第81回有機合成化学協会関東支部シンポジウム, 30 Nov. 2024, 30 Nov. 2024, 30 Nov. 2024, Domestic conference
• 糖鎖分子プローブを用いた糖質関連酵素の簡便な活性検出法の開発研究, 石井 希実, 第43回日本糖質学会年会, 12 Sep. 2024, 12 Sep. 2024, 14 Sep. 2024
• Detecting activity of Endo-S, an ENGase selectively hydrolyzes the glycans on IgG, 石井希実、野口智恵、井上周征、松尾一郎, 日本農芸化学会2024年度大会, 25 Mar. 2024, 24 Mar. 2024, 27 Mar. 2024, Domestic conference
• 有機合成化学を基盤とした糖質関連酵素の活性検出系の確立, 石井 希実, 2023年度高分子学会東北支部会員増強講演会, 13 Sep. 2023
• Synthesis of the glycopeptides for evaluating the substrate specificity of peptide:N-glycanase, 石井 希実; 高橋 諭; 佐野 加苗; 高橋 剛; 平山 弘人; 立田 由里子; 鈴木 匡; 松尾 一郎, 第42回日本糖質学会, 07 Sep. 2023, 07 Sep. 2023, 09 Sep. 2023
• Evaluation of PNGase activities using glycopeptide carrying unnatural oligosaccharides, Satoshi TAKAHASHI, Nozomi ISHII, Kanae SANO, Tatsuya UCHIBAYASHI, Tsuyoshi TAKAHASHI, Tadashi SATOH, Hirokazu YAGI, Koichi KATO, Tadashi SUZUKI, Ichiro MATSUO, 日本農芸化学会2023年度大会, 14 Mar. 2023, 14 Mar. 2023, 17 Mar. 2023
• Development of chemical probes for the cytosolic enzymes relating to N-glycan degradation, Ishii Nozomi, 第45回日本分子生物学会年会, 30 Nov. 2022, 30 Nov. 2022, 02 Dec. 2022, Domestic conference
• Synthesis of fluorogenic glycan probes for detecting ENGases activity, Nozomi Ishii, Ichiro Matsuo, International Congress on Pure & Applied Chemistry Kota Kinabalu (ICPAC KK) 2022, 23 Nov. 2022, 22 Nov. 2022, 27 Nov. 2022, English, Kota Kinabalu, Sabah, Malaysia, Malaysia, International conference
• 蛍光糖鎖プローブを用いたENGaseの基質特異性解析, 石井 希実, 井上 周征, 栗原 瑞穂, 高橋 諭, 松尾 一郎, 第16回東北糖鎖研究会, 15 Oct. 2022, 15 Oct. 2022, 16 Oct. 2022, Domestic conference
• ENGase活性の定量的評価に向けた蛍光標識糖鎖プローブの合成, 石井 希実, 松尾 一郎, 第41回日本糖質学会年会, 29 Sep. 2022, 29 Sep. 2022, 01 Oct. 2022
• Synthesis of an inhibitor based on catalytic mechanism of ENGase, ISHII Nozomi, SATO Itsuki, TAKAHASHI Satoshi, MATSUO Ichiro, 農芸化学会2022年度大会, 16 Mar. 2022, 15 Mar. 2022, 18 Mar. 2022
• FRET-based glycan probes for evaluation of ENGases activity, Nozomi Ishii, Itsuki Sato, Satoshi Takahashi, Kanae Sano, Mitsuo Nagata, Tomomichi Takagaki, and Ichiro Matsuo, Pacifichem 2021, 17 Dec. 2021, 16 Dec. 2021, 21 Dec. 2021, English, Virtual Congress (Honolulu), International conference
• エンド型糖加水分解酵素の活性検出に向けた複合型糖鎖分子プローブの合成, 石井希実、高橋 諭、佐野加苗、佐藤 樹、 松尾一郎, 第81回有機合成化学協会関東支部シンポジウム, 27 Nov. 2021, Domestic conference
• Measurements of ENGase activity using fluorescence-labeled biantennary complex type glycan probes, Nozomi Ishii, Mitsuo Nagata, Tomomichi Takagaki, Kanae Itsuki Sato, Sano, Satoshi Takahashi, Ryuji Ebashi, Ichiro Matsuo, The 40th Annual Meeting of Japanese Society of Carbohydrate Research, 29 Oct. 2021, 27 Oct. 2021, 29 Oct. 2021, Domestic conference
• Synthesis of fucosylated N-glycan probe for detecting of core fucose-specific ENGase activity, Nozomi Ishii, Mitsuo Nagata, Tomomichi Takagaki, Kanae Sano, Satoshi Takahashi, Ryuji Ebashi, Itsuki Sato, Ichiro Matsuo, 第15回東北糖鎖研究会, 25 Sep. 2021, Domestic conference
• Synthesis of a fucosylated complex type decasaccharide derivative, ISHII Nozomi, NAGATA Mitsuo, TAKAGAKI Tomomichi, TAKAHASHI Satoshi, SATO Itsuki, MATSUO Ichiro, 日本農芸化学会2021年度大会, 19 Mar. 2021, 18 Mar. 2021, 21 Mar. 2021
• 化学修飾糖鎖を基質としたENGaseの糖転移反応, 石井希実; 須永千恵; 佐野加苗; 熊田純一; 松崎祐二; 松尾一郎, 東北糖鎖研究会・東京糖鎖研究会合同シンポジウム, 18 Nov. 2017, 18 Nov. 2017, 19 Nov. 2017
• 新規ENGase探索に向けた合成化学的アプローチ, 石井 希実; 永田 光穂; 佐野 加苗; 松尾 一郎, 第８回グライコバイオロジクス研究会, 17 Nov. 2017
• 新規ENGase探索のための糖鎖プローブの開発, 石井 希実; 須永 千恵; 永田 光穂; 佐野 加苗; 松尾 一郎, 第4回FCCAシンポジウム・グライコサイエンス若手フォーラム, 28 Oct. 2017
• Synthesis of a high-mannnose type heptasaccharide probe for detection of Endo-H activity, 石井 希実; 須永 千恵; 佐野 加苗; 熊田 純一; 松崎 祐二; 松尾 一郎, 第36回日本糖質学会年会, 19 Jul. 2017, 19 Jul. 2017, 21 Jul. 2017
• Synthesis of glycan probe for evaluating the transglycosylation activity of ENGase, 石井 希実; 須永 千恵; 吉村 弥生; 熊田 純一; 松崎 祐二; 松尾 一郎, 日本農芸化学会2017年度大会, 19 Mar. 2017, 17 Mar. 2017, 20 Mar. 2017
• 水酸基の配向が異なる異性体糖鎖を用いたendo-Mの糖鎖構造認識の解析, 石井希実; 岩本将吾; 熊田純一; 松崎祐二; 松尾一郎, 第8回東北糖鎖研究会, 10 Oct. 2014, 10 Oct. 2014, 11 Oct. 2014
• Endo-MのN-型糖鎖コア構造認識に関する研究, 石井希実; 岩本将吾; 熊田純一; 松崎祐二; 松尾一郎, 日本農芸化学会2014年度大会, 30 Mar. 2014, 27 Mar. 2014, 30 Mar. 2014
• 化学合成糖鎖を基質としたendo-Mの基質特異性解析, 石井希実; 松尾一郎, 第4回グライコバイオロジクス研究会, 20 Nov. 2013

Industrial Property Rights

• Patent right, 特願2024-046089, 22 Mar. 2024
• Patent right, 特願2023-122231, Jul. 2023
• Patent right, 特願2022-195299, 07 Dec. 2022
• Patent right, 特願2018-188693, 03 Oct. 2018, 特開2020-55786, 09 Apr. 2020, 特許第7198482号, 21 Dec. 2022, Yes, Yes
• Patent right, 特願2016-118055, 14 Jun. 2016, 特開2017-222590, 21 Dec. 2017, 第6798085号, 24 Nov. 2020, Yes

Awards

• 12 Sep. 2024, Japan society, Japan
• Glyco26 Poster Awards, Evaluating the Substrate Specificity of PNGases Using Synthetic Glycopeptides, Glyco26 Local Organizing Committee, 01 Sep. 2023, International society, Taiwan, Province of China
• 07 Sep. 2023, Japan society
• 29 Sep. 2022, Japan society
• Jul. 2017

Research Projects

• 01 Apr. 2024, 31 Mar. 2025, Principal investigator, Competitive research funding, 0361086-A
• Apr. 2016, Mar. 2017, Principal investigator

Social Contribution

Social Contribution

• 高校生サイエンスインストラクター育成プログラム, 23 Aug. 2022, 10 Oct. 2022　(Target:　High school students)

Academic Contribution

• 第17回東北糖鎖研究会, 11 Nov. 2023, 12 Nov. 2023
• 第17回東北糖鎖研究会　第2回東日本糖質若手シンポジウム, Competition etc, 12 Nov. 2023, 12 Nov. 2023