Life sciences, Functional biochemistry

Life sciences, Cell biology

Life sciences, Molecular biology

Hepatocyte

Polyploid

Cytoskeleton

Intermediate filaments

Cytokinesis

differentiation

regeneration

stem cell

proteomics

signal transduction

Conformation and cooperative order-disorder transition in aqueous solutions of β-1,3-D-glucan with different degree of branching varied by the Smith degradation, Kazuto Yoshiba; Toshihiko Saheki; Bjorn E. Christensen; Toshiaki Dobashi, Jun. 2019, Biopolymers, 110, e23315

Triple Helical Conformation in Aqueous Solutions of β-1,3-D-Glucans with Varyling the Degree of Branching by the Smigh Degradation, Yoshiba Kazuto; Saheki Toshihiko; Christensen Gjorn E; Dobashi Toshiaki, 2019, ACS Applied Bio Materials

The Student Exchange Program Between Gunma University and New York State University at Stony Brock, SAHEKI Toshihiko, 2015, Journal of Jsee, 63, 5, 5_117, 5_121

Carcinogen adsorbent prepared from DNA complex by gamma-ray irradiation, Kazuya Furusawa; Erika Kita; Toshihiko Saheki; Naotsugu Nagasawa; Norio Nishi; Toshiaki Dobashi, 2008, JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 19, 9, 1159, 1170, Scientific journal

2008, 7, 4, 9, 12

細胞の運命を操るために, 佐伯俊彦, 2006, 科技振セミナー講演集

2004, 3, 4, 13, 14

OROTATE PHOSPHORIBOSYLTRANSFERASE FROM THERMUS-THERMOPHILUS - OVEREXPRESSION IN ESCHERICHIA-COLI, PURIFICATION AND CHARACTERIZATION, J BUNNAK; H HAMANA; Y OGINO; T SAHEKI; A YAMAGISHI; T OSHIMA; T DATE; T SHINOZAWA, Dec. 1995, JOURNAL OF BIOCHEMISTRY, 118, 6, 1261, 1267, Scientific journal

REGULATION FACTOR FOR PLANARIAN REGENERATION, T SHINOZAWA; S SHIOZAKI; M EZAKI; H FUJINO; T TANAKA; T SAHEKI, Jun. 1995, HYDROBIOLOGIA, 305, 1-3, 247, 253, Scientific journal

Induction of PC12 Cell Death, Apoptosis, by a Sialoglycopeptide from Bovine Brain., Kobayashi, Y; Saheki, T; Shinozawa, T, 1994, Biochem. Biophys. Res. Commun., 203, 1554, 1559

PRIMARY STRUCTURE OF THE HUMAN ELAFIN PRECURSOR PREPROELAFIN DEDUCED FROM THE NUCLEOTIDE-SEQUENCE OF ITS GENE AND THE PRESENCE OF UNIQUE REPETITIVE SEQUENCES IN THE PROSEGMENT, T SAHEKI; F ITO; H HAGIWARA; Y SAITO; J KUROKI; S TACHIBANA; S HIROSE, May 1992, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 185, 1, 240, 245, Scientific journal

1992, 8, 258, 263

STRUCTURE OF THE BOVINE ATRIAL-NATRIURETIC-PEPTIDE RECEPTOR (TYPE-C) GENE, T SAHEKI; T MIZUNO; T IWATA; Y SAITO; T NAGASAWA; KU MIZUNO; F ITO; T ITO; H HAGIWARA; S HIROSE, Jun. 1991, JOURNAL OF BIOLOGICAL CHEMISTRY, 266, 17, 11122, 11125, Scientific journal

IMMUNOCHEMICAL AND BIOCHEMICAL DISTINCTION OF SUBTYPES OF ATRIAL NATRIURETIC PEPTIDE RECEPTOR, T SAHEKI; M SHIMONAKA; K UCHIDA; T MIZUNO; S HIROSE, Oct. 1989, JOURNAL OF BIOCHEMISTRY, 106, 4, 627, 632, Scientific journal

1989, 7, 461, 466

INHIBITION OF ATRIAL NATRIURETIC PEPTIDE-INDUCED CYCLIC-GMP ACCUMULATION IN THE BOVINE ENDOTHELIAL-CELLS WITH ANTI-ATRIAL NATRIURETIC PEPTIDE RECEPTOR ANTISERUM, M ISHIDO; T FUJITA; M SHIMONAKA; T SAHEKI; S OHUCHI; T KUME; ISHIGAKI, I; S HIROSE, Jan. 1989, JOURNAL OF BIOLOGICAL CHEMISTRY, 264, 1, 641, 645, Scientific journal

VISUALIZATION OF ANP RECEPTOR ON GLOMERULI OF BOVINE KIDNEY BY USE OF A SPECIFIC ANTISERUM, M SHIMONAKA; T SAHEKI; H HAGIWARA; Y HAGIWARA; H SONO; S HIROSE, Nov. 1987, AMERICAN JOURNAL OF PHYSIOLOGY, 253, 5, F1058, F1062

IDENTIFICATION OF THE PRIMARY TRANSLATION PRODUCT OF ATRIAL-NATRIURETIC-PEPTIDE RECEPTOR MESSENGER-RNA IN A CELL-FREE SYSTEM USING ANTIRECEPTOR ANTISERUM, K UCHIDA; M SHIMONAKA; T SAHEKI; T ITO; S HIROSE, Sep. 1987, JOURNAL OF BIOLOGICAL CHEMISTRY, 262, 26, 12401, 12402

PURIFICATION OF ATRIAL-NATRIURETIC-PEPTIDE RECEPTOR FROM BOVINE LUNG - EVIDENCE FOR A DISULFIDE-LINKED SUBUNIT STRUCTURE, M SHIMONAKA; T SAHEKI; H HAGIWARA; M ISHIDO; A NOGI; T FUJITA; K WAKITA; Y INADA; J KONDO; S HIROSE, Apr. 1987, JOURNAL OF BIOLOGICAL CHEMISTRY, 262, 12, 5510, 5514, Scientific journal

PHYSICAL AND FUNCTIONAL ASSOCIATION OF THE ATRIAL-NATRIURETIC-PEPTIDE RECEPTOR WITH PARTICULATE GUANYLATE-CYCLASE AS DEMONSTRATED USING DETERGENT EXTRACTS OF BOVINE LUNG MEMBRANES, M ISHIDO; T FUJITA; H HAGIWARA; M SHIMONAKA; T SAHEKI; Y HIRATA; S HIROSE, Oct. 1986, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 140, 1, 101, 106, Scientific journal

Miniaturized two-dimensional gel electrophoresis of high-molecular-weight proteins using low-concentration multifilament-supporting gel for isoelectric focusing, ODAKE Tamao;SAHEKI Toshihiko;KAMEZAWA Kazutoshi;MIYATA Kozue;TAKIGUCHI Hiromi;HOTTA Hiroki;ONDA Koki;KOJIMA Yasuaki;LI Jinxiang;TSUNODA Kin-ichi, Dec. 2009, Journal of Electrophoresis, 53, 4, 57, 61

Fast separation of large proteins with two-dimensional gel electrophoresis using a low-concentration polyacrylamide gel supported by a loose multifilament string, Saheki Toshihiko;Odake Tamao;Kojima Yasuaki;Tsunoda Kin-ichi, 2005, Abstracts for Annual Meeting of Japanese Proteomics Society, 2005, 0, 95, 95

Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation., Saheki Toshihiko;Ito Hitomi;Sekiguchi Akihiro;Nishina Atsuyoshi;Sugiyama Toshihiro;Izumi Takashi;Kojima Itaru, 15 Jan. 2008, Cytotechnology, 57, 2

A novel partial agonist of GPBA reduces blood glucose level in a murine glucose tolerance test., Enomoto Rina;Kurosawa Aya;Nikaido Yoshiaki;Mashiko Misaki;Saheki Toshihiko;Nakajima Nozomi;Kuroiwa Satoshi;Otobe Michinari;Ohsaki Maki;Tooyama Kazuya;Inoue Yusuke;Kuwabara Nobuo;Kikuchi Osamu;Kitamura Tadahiro;Kojima Itaru;Nakagawa Yuko;Saito Tamio;Osada Hiroyuki;Futahashi Mizuko;Sezutsu Hideki;Takeda Shigeki, 17 Aug. 2017, European journal of pharmacology, 814

Conformation and cooperative order-disorder transition in aqueous solutions of β-1,3-d-glucan with different degree of branching varied by the Smith degradation., Yoshiba Kazuto;Saheki Toshihiko;Christensen Bjørn E;Dobashi Toshiaki, 10 Jun. 2019, Biopolymers, 110, 9

;, Nov. 2022, 95回, 2P

A novel partial agonist of GPBA reduces blood glucose level in a murine glucose tolerance test, Rina Enomoto; Aya Kurosawa; Yoshiaki Nikaido; Misaki Mashiko; Toshihiko Saheki; Nozomi Nakajima; Satoshi Kuroiwa; Michinari Otobe; Maki Ohsaki; Kazuya Tooyama; Yusuke Inoue; Nobuo Kuwabara; Osamu Kikuchi; Tadahiro Kitamura; Itaru Kojima; Yuko Nakagawa; Tamio Saito; Hiroyuki Osada; Mizuko Futahashi; Hideki Sezutsu; Shigeki Takeda, 05 Nov. 2017, European Journal of Pharmacology, 814, 130, 137

A novel partial agonist of GPBA reduces blood glucose level in a murine glucose tolerance test, Rina Enomoto; Aya Kurosawa; Yoshiaki Nikaido; Misaki Mashiko; Toshihiko Saheki; Nozomi Nakajima; Satoshi Kuroiwa; Michinari Otobe; Maki Ohsaki; Kazuya Tooyama; Yusuke Inoue; Nobuo Kuwabara; Osamu Kikuchi; Tadahiro Kitamura; Itaru Kojima; Yuko Nakagawa; Tamio Saito; Hiroyuki Osada; Mizuko Futahashi; Hideki Sezutsu; Shigeki Takeda, Nov. 2017, EUROPEAN JOURNAL OF PHARMACOLOGY, 814, 130, 137

2015, 63, 5, 117, 121

2014, 14, 1, 29, 32

Genomic and proteomic characterization of the largeMyoviridaebacteriophage ϕTMA of the extreme thermophileThermus thermophilus, Masatada Tamakoshi; Aya Murakami; Motoki Sugisawa; Kenji Tsuneizumi; Shigeki Takeda; Toshihiko Saheki; Takashi Izumi; Toshihiko Akiba; Kaoru Mitsuoka; Hidehiro Toh; Atsushi Yamashita; Fumio Arisaka; Masahira Hattori; Tairo Oshima; Akihiko Yamagishi, May 2011, Bacteriophage, 1, 3, 152, 164

2011, 36, 3, 393, 396

Additive Effects of Betains on the Fibrinogen cryogelation Induced by Low Temperature, Y. Toyama; K. Miyamoto; K. Kubota; K. Wakamatsu; N. Nameki; T. Saheki; M. Ochiai, 2011, Transactions of the Materials Research Society of Japan, 36, 3, 393, 396

酪酸ナトリウムでラット肝臓幹様細胞のAnnexin A3の発現が抑制される(Annexin A3 mRNA repression by sodium butyrate in rat hepatic stem-like cells), 斎藤 健一; 富澤 彰太; 向井 裕輔; 岸 美紀子; 和泉 孝志; 杉山 俊博; 小島 至; 佐伯 俊彦, Dec. 2010, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 83回・33回, 3P, 0868

2010, 59th

Miniaturized two-dimensional gel electrophoresis of high-molecular-weight proteins using low-concentration multifilament-supporting gel for isoelectric focusing, Tamao Odake; Toshihiko Saheki; Kazutoshi Kamezawa; Kozue Miyata; Hiromi Takiguchi; Hiroki Hotta; Koki Onda; Yasuaki Kojima; Jinxiang Li; Kin-ichi Tsunoda, 2010, Journal of Electrophoresis, 53, 4, 57, 61

Miniaturized two-dimensional gel electrophoresis of high-molecular-weight proteins using low-concentration multifilament-supporting gel for isoelectric focusing, Odake T; Saheki T; Kamezawa K; Miyata K; Takiguchi H; Hotta H; Onda K; Kojima Y; Li J; Tsunoda K, 2010, J. Electrophoresis, 53, 4, 57, 61

酪酸ナトリウムに対して可逆的に反応するラット肝臓幹様細胞のプロテオーム解析, 向井 裕輔; 斎藤 健一; 岸 美紀子; 和泉 孝志; 杉山 俊博; 小島 至; 佐伯 俊彦, Sep. 2009, 日本生化学会大会プログラム・講演要旨集, 82回, 2P, 769

2009, 2009

2009, 53, 3

2009, 16

Study of butyrate signal transduction pathways in rat hepatic stem-like cells, Saheki T; Mukai Y; Saito K; Tajima E; Katakura K; Nishina A; Kishi M; Izumi T; Sugiyama T; Kojima I, 2009, Animal Cell Technol.: Basic & Applied Aspects, Proceedings of the 21st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT), 16

Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation, Toshihiko Saheki; Hitomi Ito; Akihiro Sekiguchi; Atsuyoshi Nishina; Toshihiro Sugiyama; Takashi Izumi; Itaru Kojima, Jun. 2008, CYTOTECHNOLOGY, 57, 2, 137, 143

2008, 2007

2008, 69th

2008, 52, 3

2007, 2007

2007, 56th

Leap to the future!, Contributor, The Secret of the Liver, Sep. 2021

Leap to the futre!, Contributor, Are you not Good at English?, Apr. 2021

Leap to the Future! 1 (2022), Contributor, Mutsumi Iijima, Are you not Good at English?, 31 Mar. 2021, 124, 60-63, Textbook

Leap to the Future! 2 (2022), Contributor, Mutsumi Iijima, Are you not Good at English?, 31 Mar. 2021, 124, 60-63, Textbook

20 Sep. 2019, English, Domestic conference

24 Jun. 2019, English, Domestic conference

2017

2015

2015

2014

2014

2013

2013

Proteomic analysis of polyploid cells derived from rat hepatic stem-like cells, The Japanese Biochemical Society, 2013

2012

2012

2012

Analysis of histone H3 acetylation in rat hepatic stem-like cells during multiple nucleation, Japanese Association for Animal Cell Technology, 2012

2011

2011

Acquisition of multiple nuclei by sodium butyrate treatment of rat hepatic stem-like cells, The Molecular Biology Society of Japan, 2011

2010

2009

2009

2009

Insulin restores decreased p53 mRNA expression during polynucleated cells induction using a histone deacetylase inhibitor, Haruka Kimura, Toshihiko Saheki, The 95th Annual Meeting of the Japanease Biochemical Society, 10 Nov. 2022, 09 Nov. 2022, 11 Nov. 2022

Insulin affects p21 expression to promote polynuclear rat hepatic stem-like cell induction by histone deacetylase inhibitor, Toshihiko Saheki, Haruka Kimura, The 74th Annual Meeting of the Japan Society for Cell Biology, 28 Jun. 2022, 28 Jun. 2022, 30 Jun. 2022

04 Dec. 2021, 04 Dec. 2021, 04 Dec. 2021, Japanese, Japan, Domestic conference

Development of High-Performance Two-Dimensional Electrophoresis Using Low-Concentration Gel and Its Application, ODAKE Tamao; TSUNODA Kin-ichi; SAHEKI Toshihiko; UEKI Yuji, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), The University of Tokyo, 2008, 2010, The researchers have developed a new isoelectric focusing(IEF) gel supported by a multifilament as a first-dimensional IEF gel of two-dimensional electrophoresis(2-DE), which can separate proteins with high-resolution. The multifilament-supporting(MFS) gel could overcome difficulty in handling the IEF gel, prepare low-concentration gel to separate high-molecular-weight proteins. In this study, to improve operability and reproducibility, support of the low-concentration gel, method of polymerization, and introduction of immobilized pH gradient were investigated. The 2-DE system using the low-concentration MFS gel was proved to be useful for proteome analysis., 20550072, kaken

Development of High-Performance Two-Dimensional Gel Electrophoresis Using Low-Concentration Polyacrylamide Gel Supported by Multifilament Yarns, ODAKE Tamao; TSUNODA Kin-ichi; SAHEKI Toshihiko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 2005, 2006, We proposed multifilament-supporting gel (MFS gel) as a novel first-dimensional isoelectric focusing (IEF) gel of two-dimensional gel electrophoresis (2-DE). The MFS gel could reduce concentration of polyacrylamide down to 2%T (conventionally 4%T), which would provide large pore of the gel, resulting in fast IEF and separation of high-molecular-weight (HMW) proteins up to 200 kDa. However, due to carrier ampholyte added to form pH gradient, stability and reproducibility was not sufficient. The aim of this research was to improve the pH stability and resolution by optimization of the MFS gel and reduction of polyacrylamide gel concentration, to obtain fast 2-DE separation of HMW proteins. 1. Stabilize pH gradient of the MFS gel. Immobilized pH gradient (IPG) method is known that it provides a stable pH gradient during even a long IEF time. To improve pH stability, IEF method was introduced to the MFS gel. IPG MFS gel was prepared. Further investigation on controlling flow rate and instrumental improvement was still necessary to obtain wider pH range and gel-to-gel reproducibility. 2. Lower the MFS gel concentration Polyacrylamide gel of lower concentration has larger pore and is expected to provide fast IEF and separation of HMW proteins. Polymerization by heat could provide 1.75%T polyacrylamide, but too fragile for practical use. Addition of agarose was found to be effective to reinforce the low-concentration MFS gel to some degree. Radiation is an alternative to promote polymerization of acrylamide, which effectively produces radicals. We utilized Co-60 gamma ray to prepare MFS gels of low concentration below 2%T. Polyacrylamide of 1.5%T could be successfully prepared and applied to IEF. 3. Application of low-concentration MFS gel to 2-DE Developed low-concentration polyacrylamide gel was applied to 2-DE. The low-concentration polyacrylamide gels were proved to provide clearer protein spots in the HMW region., 17550072, kaken

1997, 1998, 09780716, kaken

1996, 1997, 08874122, kaken

Comparative molecular biological studies on the vasoactive peptide receptors., HIROSE Shigehisa; SAHEKI Toshihiko; HAGIWARA Hiromi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for General Scientific Research (A), Tokyo Institute of Technology, 1992, 1995, To further advance our understanding on the physiological significance of vasoactive peptides, we carried out the following comparative molecular biological studies on their receptors. 1) Natriuretic peptide receptors : The intra-and inter-subunit disulfide bonding pattern of the bovine and eel natriuretic peptide receptor C (NPR-C) were determined by a combination of protein sequencing and site-directed mutagenesis. Analysis of the cDNA and gene structures of mammalian NPR-C revealed the presence of an alternatively spliced mRNA species that encodes a receptor protein with a slightly modified sequence in the juxta-membrane region of the extracellular domain. A new receptor subtype, termed NPR-D,has been identified, cloned, and characterized. NPR-D has a short cytoplasmic tail and is 70% similar to the NPR-C sequence, but quite different in the tissue distribution ; NPR-D is abundant in the brain. 2)Endothelin receptors : Localization of the endothelin receptor B (ET-B) was determined by immunohistochemistry and the vascular endotherium was found to be decorated most densely. Type A endothelin receptor (ET-A) was found to be highly expressed in the perichondrium of rats and the gill epitherium of trout, suggesting that endothelin is involved in the differentiation of chondrocytes and regulation of gill functions., 04404082, kaken

1994, 1994, 06858074, kaken

1993, 1993, 05858097, kaken