Researcher Profile and Settings

Researcher

• Name

  HOSOI Nobutake

Affiliation

• Neurophysiology and Neural Repair, Associate Professor

Profile and Settings

• Name

  HOSOI, Nobutake

Affiliation

• Gunma University, Graduate School of Medicine Neurophysiology and Neural Repair
• Gunma University, Graduate School of Medicine Neurophysiology and Neural Repair

Affiliation

• Affiliation

  Graduate School of Medicine, Neurophysiology and Neural Repair

• Job title

  Associate Professor

Association Memberships

• THE JAPAN NEUROSCIENCE SOCIETY
• THE PHYSIOLOGICAL SOCIETY OF JAPAN
• THE ZOOLOGICAL SOCIETY OF JAPAN
• SOCIETY for NEUROSCIENCE

Research Experience

• Apr. 2004, Mar. 2009, Max Planck Institute for Biohysical Chemistry, Department of Membrane Biophysics (Erwin Neher lab), Postdoctoral fellow
• Apr. 2009, May 2015, Gunma University Graduate School of Medicine, Department of Neurophysiology, Assisrant professor
• Jun. 2015, Oct. 2020, Gunma University Graduate School of Medicine, Department of Neurophysiology and Neural Repair
• Nov. 2020, 9999, Gunma University Graduate School of Medicine, Department of Neurophysiology and Neural Repair, Associate Professor

Research Activities

Research Areas

• Life sciences, Neuroscience - general

Research Interests

• image and movie analysis
• animal behavior
• cerebellum
• retina
• Neuroscience
• Electrophysiology
• Patchclamp
• Ca imaging

Published Papers

• Calcium Dependence of Exo- and Endocytotic Coupling at a Glutamatergic Synapse, Nobutake Hosoi; Matthew Holt; Takeshi Sakaba, Jul. 2009, NEURON, 63, 2, 216, 229, Scientific journal

  DOI
• Quantitative analysis of calcium-dependent vesicle recruitment and its functional role at the calyx of held synapse, Nobutake Hosoi; Takeshi Sakaba; Erwin Neher, Dec. 2007, JOURNAL OF NEUROSCIENCE, 27, 52, 14286, 14298, Scientific journal

  DOI
• Group III metabotropic glutamate receptors and exocytosed protons inhibit L-type calcium currents in cones but not in rods, N Hosoi; Arai, I; M Tachibana, Apr. 2005, JOURNAL OF NEUROSCIENCE, 25, 16, 4062, 4072, Scientific journal

  DOI
• Disruption of metabotropic glutamate receptor signalling is a major defect at cerebellar parallel fibre-Purkinje cell synapses in staggerer mutant mice, Kazuhiro Mitsumura; Nobutake Hosoi; Nobuhiko Furuya; Hirokazu Hirai, Jul. 2011, JOURNAL OF PHYSIOLOGY-LONDON, 589, 13, 3191, 3209, Scientific journal

  DOI
• Glycine release from astrocytes via functional reversal of GlyT1, Koji Shibasaki; Nobutake Hosoi; Ryosuke Kaneko; Makoto Tominaga; Katsuya Yamada, Feb. 2017, JOURNAL OF NEUROCHEMISTRY, 140, 3, 395, 403, Scientific journal

  DOI
• Progressive impairment of cerebellar mGluR signalling and its therapeutic potential for cerebellar ataxia in spinocerebellar ataxia type 1 model mice, Anton N. Shuvaev; Nobutake Hosoi; Yamato Sato; Dai Yanagihara; Hirokazu Hirai, Jan. 2017, JOURNAL OF PHYSIOLOGY-LONDON, 595, 1, 141, 164, Scientific journal

  DOI
• Deletion of Class II ADP-Ribosylation Factors in Mice Causes Tremor by the Nav1.6 Loss in Cerebellar Purkinje Cell Axon Initial Segments., Nobutake Hosoi; Koji Shibasaki; Mayu Hosono; Ayumu Konno; Yo Shinoda; Hiroshi Kiyonari; Kenichi Inoue; Shin-Ichi Muramatsu; Yasuki Ishizaki; Hirokazu Hirai; Teiichi Furuichi; Tetsushi Sadakata, 07 Aug. 2019, The Journal of Neuroscience : the official journal of the Society for Neuroscience, 39, 32, 6339, 6353, Scientific journal

  DOI
• Active role of glutamate uptake in the synaptic transmission from retinal nonspiking neurons, K Matsui; N Hosoi; M Tachibana, Aug. 1999, JOURNAL OF NEUROSCIENCE, 19, 16, 6755, 6766, Scientific journal
• Excitatory synaptic transmission in the inner retina: Paired recordings of bipolar cells and neurons of the ganglion cell layer, K Matsui; N Hosoi; M Tachibana, Jun. 1998, JOURNAL OF NEUROSCIENCE, 18, 12, 4500, 4510, Scientific journal
• Disruption of metabotropic glutamate receptor signalling is a major defect at cerebellar parallel fibre-Purkinje cell synapses in staggerer mutant mice, Mitsumura, Kazuhiro;Hosoi, Nobutake;Furuya, Nobuhiko;Hirai, Hirokazu, 2011, JOURNAL OF PHYSIOLOGY-LONDON, 589, 13, 3191, 3209

  DOI
• Electrophysiological and Imaging Analysis of GFP-Tagged Protein Kinase C γ Translocation in Cerebellar Purkinje Cells., Hirokazu Hirai; Yuuki Fukai; Ayumu Konno; Nobutake Hosoi, 26 Feb. 2022, Cerebellum (London, England), Scientific journal

  DOI
• Protein kinase Cγ in cerebellar Purkinje cells regulates Ca2+-activated large-conductance K+ channels and motor coordination., Masashi Watanave; Nobutaka Takahashi; Nobutake Hosoi; Ayumu Konno; Hikaru Yamamoto; Hiroyuki Yasui; Mika Kawachi; Takuro Horii; Yasunori Matsuzaki; Izuho Hatada; Hirokazu Hirai, 15 Feb. 2022, Proceedings of the National Academy of Sciences of the United States of America, 119, 7, Scientific journal

  DOI
• The neurotoxic effect of lactational PFOS exposure on cerebellar functional development in male mice., Ayane Ninomiya; Abdallah Mshaty; Asahi Haijima; Hiroyuki Yajima; Michifumi Kokubo; Miski Aghnia Khairinisa; Winda Ariyani; Yuki Fujiwara; Sumiyasu Ishii; Nobutake Hosoi; Hirokazu Hirai; Izuki Amano; Noriyuki Koibuchi, Jan. 2022, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 159, 112751, 112751, Scientific journal

  DOI
• GABAergic neuron-specific whole-brain transduction by AAV-PHP.B incorporated with a new GAD65 promoter, Chiaki Hoshino; Ayumu Konno; Nobutake Hosoi; Ryosuke Kaneko; Ryo Mukai; Junichi Nakai; Hirokazu Hirai, Dec. 2021, Molecular Brain, 14, 1, Scientific journal

  DOI
• 甲状腺ホルモン受容体による小脳プルキンエ細胞におけるシナプス可塑性の調節機構, 二ノ宮 彩音; 細井 延武; 小久保 倫文; 天野 出月; はい島 旭; 宮崎 航; 平井 宏和; 鯉淵 典之, Feb. 2020, 95, 4, 1342, 1342
• Activation of group III metabotropic glutamate receptor suppresses the membrane currents in newt photoreceptors, N Hosoi; J Hong; M Tachibana, 2003, NEURAL BASIS OF EARLY VISION, 11, 96, 96, International conference proceedings
• GABAergic neuron-specific whole-brain transduction by AAV-PHP.B incorporated with a new GAD65 promoter, Hoshino, Chiaki;Konno, Ayumu;Hosoi, Nobutake;Kaneko, Ryosuke;Mukai, Ryo;Nakai, Junichi;Hirai, Hirokazu, 2021, MOLECULAR BRAIN, 14, 1

  DOI
• Deletion of Class II ADP-Ribosylation Factors in Mice Causes Tremor by the Nav1.6 Loss in Cerebellar Purkinje Cell Axon Initial Segments, Hosoi, Nobutake;Shibasaki, Koji;Hosono, Mayu;Konno, Ayumu;Shinoda, Yo;Kiyonari, Hiroshi;Inoue, Kenichi;Muramatsu, EShin-ichi;Ishizaki, Yasuki;Hirai, Hirokazu;Furuichi, Telichi;Sadakata, Tetsushi, 2019, JOURNAL OF NEUROSCIENCE, 39, 32, 6339, 6353

  DOI
• Glycine release from astrocytes via functional reversal of GlyT1, Shibasaki, Koji;Hosoi, Nobutake;Kaneko, Ryosuke;Tominaga, Makoto;Yamada, Katsuya, 2017, JOURNAL OF NEUROCHEMISTRY, 140, 3, 395, 403

  DOI
• Progressive impairment of cerebellar mGluR signalling and its therapeutic potential for cerebellar ataxia in spinocerebellar ataxia type 1 model mice, Shuvaev, Anton N.;Hosoi, Nobutake;Sato, Yamato;Yanagihara, Dai;Hirai, Hirokazu, 2017, JOURNAL OF PHYSIOLOGY-LONDON, 595, 1, 141, 164

  DOI
• Progressive impairment of cerebellar mGluR signalling and its therapeutic potential for cerebellar ataxia in spinocerebellar ataxia type 1 model mice, Shuvaev, Anton N.;Hosoi, Nobutake;Sato, Yamato;Yanagihara, Dai;Hirai, Hirokazu, 2017, JOURNAL OF PHYSIOLOGY-LONDON, 595, 1, 141, 164

  DOI
• Impairment of mGluR signaling at cerebellar parallel fiber-Purkinje cell synapses in staggerer mutant mice, Hosoi, Nobutake;Mitsumura, Kazuhiro;Hirai, Hirokazu, 2011, NEUROSCIENCE RESEARCH, 71, E246, E246

  DOI
• Electrophysiological and Imaging Analysis of GFP-Tagged Protein Kinase C gamma Translocation in Cerebellar Purkinje Cells, Hirai, Hirokazu;Fukai, Yuuki;Konno, Ayumu;Hosoi, Nobutake, 2022, CEREBELLUM, 21, 5, 776, 783

  DOI
• Protein kinase C gamma in cerebellar Purkinje cells regulates Ca2+-activated large-conductance K+ channels and motor coordination, Watanave, Masashi;Takahashi, Nobutaka;Hosoi, Nobutake;Konno, Ayumu;Yamamoto, Hikaru;Yasui, Hiroyuki;Kawachi, Mika;Horii, Takuro;Matsuzaki, Yasunori;Hatada, Izuho;Hirai, Hirokazu, 2022, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119, 7

  DOI
• The neurotoxic effect of lactational PFOS exposure on cerebellar functional development in male mice, Ninomiya, Ayane;Mshaty, Abdallah;Haijima, Asahi;Yajima, Hiroyuki;Kokubo, Michifumi;Khairinisa, Miski Aghnia;Ariyani, Winda;Fujiwara, Yuki;Ishii, Sumiyasu;Hosoi, Nobutake;Hirai, Hirokazu;Amano, Izuki;Koibuchi, Noriyuki, 2022, FOOD AND CHEMICAL TOXICOLOGY, 159

  DOI
• 神経変性疾患の最前線2016　運動の記憶を形成する細胞内シグナル　脊髄小脳変性症マウスを用いた解析と治療の可能性(Signaling pathway required for motor memory formation: Studies using spinocerebellar ataxia model mice and the therapeutic perspective), ;;, Jul. 2016, 46回, 84
• 【小脳研究の未来】病態　遺伝子改変疾患マウスと小脳異常, ;, Feb. 2021, 72, 1, 69
• モデルマウスを用いた本態性振戦発症機序の解明, ;;;;;;;, Aug. 2018, 68, 3, 211
• 甲状腺ホルモン受容体による小脳プルキンエ細胞におけるシナプス可塑性の調節機構, ;;;;;;;, Feb. 2020, 95, 4, 1342
• クラスIIARFタンパク質は、小脳プルキンエ細胞の軸索起始部へのNav1.6チャネルの輸送に関与する, ;;;;;;, Sep. 2019, 92回, [1T14a
• 小脳機能維持における甲状腺ホルモンの新たな役割の解明　プルキンエ細胞小胞体からのカルシウム放出調節における重要性, ;;;;;;, Apr. 2021, 97, 1, 265
• Disruption of metabotropic glutamate receptor signalling is a major defect at cerebellar parallel fibre-Purkinje cell synapses in staggerer mutant mice., Mitsumura Kazuhiro;Hosoi Nobutake;Furuya Nobuhiko;Hirai Hirokazu, May 2011, The Journal of physiology, 589, Pt 13

  DOI
• クラスIIARFタンパク質は、小脳プルキンエ細胞の軸索起始部へのNav1.6チャネルの輸送に関与する, ;;;;;;, Sep. 2019, 92回, [1T14a
• プルキンエ細胞特異的に発現させた変異甲状腺ホルモン受容体の運動機能調節における作用, ;;;;;, Apr. 2022, 98, 1, 317
• Glycine release from astrocytes via functional reversal of GlyT1., Shibasaki Koji;Hosoi Nobutake;Kaneko Ryosuke;Tominaga Makoto;Yamada Katsuya, Aug. 2016, Journal of neurochemistry, 140, 3

  DOI
• Progressive impairment of cerebellar mGluR signalling and its therapeutic potential for cerebellar ataxia in spinocerebellar ataxia type 1 model mice., Shuvaev Anton N;Hosoi Nobutake;Sato Yamato;Yanagihara Dai;Hirai Hirokazu, Sep. 2016, The Journal of physiology, 595, 1

  DOI
• Deletion of Class II ADP-Ribosylation Factors in Mice Causes Tremor by the Nav1.6 Loss in Cerebellar Purkinje Cell Axon Initial Segments., Hosoi Nobutake;Shibasaki Koji;Hosono Mayu;Konno Ayumu;Shinoda Yo;Kiyonari Hiroshi;Inoue Kenichi;Muramatsu Shin-Ichi;Ishizaki Yasuki;Hirai Hirokazu;Furuichi Teiichi;Sadakata Tetsushi, Jun. 2019, The Journal of neuroscience : the official journal of the Society for Neuroscience, 39, 32

  DOI
• GABAergic neuron-specific whole-brain transduction by AAV-PHP.B incorporated with a new GAD65 promoter., Hoshino Chiaki;Konno Ayumu;Hosoi Nobutake;Kaneko Ryosuke;Mukai Ryo;Nakai Junichi;Hirai Hirokazu, Feb. 2021, Molecular brain, 14, 1

  DOI
• The neurotoxic effect of lactational PFOS exposure on cerebellar functional development in male mice., Ninomiya Ayane;Mshaty Abdallah;Haijima Asahi;Yajima Hiroyuki;Kokubo Michifumi;Khairinisa Miski Aghnia;Ariyani Winda;Fujiwara Yuki;Ishii Sumiyasu;Hosoi Nobutake;Hirai Hirokazu;Amano Izuki;Koibuchi Noriyuki, Dec. 2021, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 159

  DOI
• Protein kinase Cγ in cerebellar Purkinje cells regulates Ca2+-activated large-conductance K+ channels and motor coordination., Watanave Masashi;Takahashi Nobutaka;Hosoi Nobutake;Konno Ayumu;Yamamoto Hikaru;Yasui Hiroyuki;Kawachi Mika;Horii Takuro;Matsuzaki Yasunori;Hatada Izuho;Hirai Hirokazu, Proceedings of the National Academy of Sciences of the United States of America, 119, 7

  DOI
• Electrophysiological and Imaging Analysis of GFP-Tagged Protein Kinase C γ Translocation in Cerebellar Purkinje Cells., Hirai Hirokazu;Fukai Yuuki;Konno Ayumu;Hosoi Nobutake, Feb. 2022, Cerebellum (London, England)

  DOI
• Development of microglia-targeting adeno-associated viral vectors as tools to study microglial behavior in vivo, Okada, Yukihiro;Hosoi, Nobutake;Matsuzaki, Yasunori;Fukai, Yuuki;Hiraga, Akito;Nakai, Junichi;Nitta, Keisuke;Shinohara, Yoichiro;Konno, Ayumu;Hirai, Hirokazu, 2022, COMMUNICATIONS BIOLOGY, 5, 1

  DOI
• Long-term depression-inductive stimulation causes long-term potentiation in mouse Purkinje cells with a mutant thyroid hormone receptor, Ninomiya, Ayane;Amano, Izuki;Kokubo, Michifumi;Takatsuru, Yusuke;Ishii, Sumiyasu;Hirai, Hirokazu;Hosoi, Nobutake;Koibuchi, Noriyuki, 2022, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119, 45

  DOI
• Interaction between Teneurin-2 and microtubules via EB proteins provides a platform for GABAA receptor exocytosis, Ichinose, Sotaro;Susuki, Yoshihiro;Hosoi, Nobutake;Kaneko, Ryosuke;Ebihara, Mizuho;Hirai, Hirokazu;Iwasaki, Hirohide;Ori-McKenney, Kassandra M., 2023, ELIFE, 12

  DOI
• プルキンエ細胞特異的に発現させた変異甲状腺ホルモン受容体の運動機能調節における作用, ;;;;;, Apr. 2022, 98, 1, 317
• 授乳期PFOS曝露による小脳機能の発達異常の体系的解析, ;;;;;;;;;;;;, Jul. 2022, 98, 3, 707
• 変異甲状腺ホルモン受容体を発現させたマウスプルキンエ細胞では、長期抑圧誘発刺激は長期増強を惹起する, ;;;;;;;, May 2023, 99, 1, 286
• Interaction between Teneurin-2 and microtubules via EB proteins provides a platform for GABAA receptor exocytosis., Sotaro Ichinose; Yoshihiro Susuki; Nobutake Hosoi; Ryosuke Kaneko; Mizuho Ebihara; Hirokazu Hirai; Hirohide Iwasaki, 05 Jun. 2023, eLife, 12, Scientific journal

  DOI
• 変異甲状腺ホルモン受容体を発現させたマウスプルキンエ細胞では、長期抑圧誘発刺激は長期増強を惹起する, 二ノ宮 彩音; 天野 出月; 小久保 倫文; 高鶴 裕介; 石井 角保; 平井 宏和; 細井 延武; 鯉淵 典之, May 2023, 99, 1, 286, 286
• Development of microglia-targeting adeno-associated viral vectors as tools to study microglial behavior in vivo, Yukihiro Okada; Nobutake Hosoi; Yasunori Matsuzaki; Yuuki Fukai; Akito Hiraga; Junichi Nakai; Keisuke Nitta; Yoichiro Shinohara; Ayumu Konno; Hirokazu Hirai, 11 Nov. 2022, Communications Biology, 5, 1, Scientific journal

  DOI
• Long-term depression-inductive stimulation causes long-term potentiation in mouse Purkinje cells with a mutant thyroid hormone receptor., Ayane Ninomiya; Izuki Amano; Michifumi Kokubo; Yusuke Takatsuru; Sumiyasu Ishii; Hirokazu Hirai; Nobutake Hosoi; Noriyuki Koibuchi, 08 Nov. 2022, Proceedings of the National Academy of Sciences of the United States of America, 119, 45, e2210645119, Scientific journal

  DOI
• 授乳期PFOS曝露による小脳機能の発達異常の体系的解析, 二ノ宮 彩音; Abdallah Mshaty; はい島 旭; 矢島 弘之; 小久保 倫文; Aghnia Khairinisa Miski; Ariyani Winda; 藤原 悠基; 石井 角保; 細井 延武; 平井 宏和; 天野 出月; 鯉淵 典之, Jul. 2022, 98, 3, 707, 707
• プルキンエ細胞特異的に発現させた変異甲状腺ホルモン受容体の運動機能調節における作用, 二ノ宮 彩音; 天野 出月; 石井 角保; 平井 宏和; 細井 延武; 鯉淵 典之, Apr. 2022, 98, 1, 317, 317
• 2SC05 Feedback Regulation of Ca Current in Photoreceptors, Tachibana M; Hosoi N; Arai I, 2004, Seibutsu Butsuri, 44, S16

  DOI
• Development of an AAV Vector System for Highly Specific and Efficient Gene Expression in Microglia, Ryo Aoki; Ayumu Konno; Nobutake Hosoi; Hayato Kawabata; Hirokazu Hirai, 06 Nov. 2024

  DOI

MISC

• Genetically-modified diseased mice and cerebellar abnormalities, Nobutake Hosoi; Hirokazu Hirai, 15 Feb. 2021, 72, 1, 69, 73, Introduction other

  DOI
• クラスIIARFタンパク質は、小脳プルキンエ細胞の軸索起始部へのNav1.6チャネルの輸送に関与する, 定方 哲史; 細井 延武; 柴崎 貢志; 今野 歩; 平井 宏和; 石崎 泰樹; 古市 貞一, Sep. 2019, 日本生化学会大会プログラム・講演要旨集, 92回, [1T14a, 01]
• モデルマウスを用いた本態性振戦発症機序の解明, 細井 延武; 柴崎 貢志; 今野 歩; 村松 慎一; 石崎 泰樹; 平井 宏和; 古市 貞一; 定方 哲史, Aug. 2018, 68, 3, 211, 211
• モデルマウスを用いた本態性振戦発症機序の解明, 細井延武; 柴崎貢志; 今野歩; 村松慎一; 石崎泰樹; 平井宏和; 古市貞一; 定方哲史, Aug. 2018, 北関東医学会総会プログラム・抄録, 65th, 3, 31, 211
• Impairment of mGluR signaling at cerebellar parallel fiber-Purkinje cell synapses in staggerer mutant mice, Nobutake Hosoi; Kazuhiro Mitsumura; Hirokazu Hirai, 2011, NEUROSCIENCE RESEARCH, 71, E246, E246, Summary international conference

  DOI
• Contribution of vesicle recruitment and dynamic change of release probability during short-term plasticity at the calyx of Held, Nobutake Hosoi; Takeshi Sakaba; Erwin Neher, 2008, NEUROSCIENCE RESEARCH, 61, S76, S76, Summary international conference
• Modeling of synaptic vesicle dynamics at the calyx of held, Erwin Neher; Nobutake Hosoi; Takeshi Sakaba, Jan. 2007, BIOPHYSICAL JOURNAL, 170A, 170A, Summary international conference
• 網膜におけるペアレコーディング, 松井 広; 細井 延武; 立花 政夫, 01 Oct. 2000, 62, 10, 279, 281
• Elucidating the Mechanisms of Life using Patchclamp Technique and Live Imaging, Hosoi Nobutake, 01 Aug. 2023, The Kitakanto Medical Journal, 73, 3, 225, 226

  DOI
• 小脳機能維持における甲状腺ホルモンの新たな役割の解明 プルキンエ細胞小胞体からのカルシウム放出調節における重要性, 二ノ宮 彩音; 細井 延武; 天野 出月; 小久保 倫文; 高鶴 裕介; 平井 宏和; 鯉淵 典之, Apr. 2021, 97, 1, 265, 265
• クラスIIARFタンパク質は、小脳プルキンエ細胞の軸索起始部へのNav1.6チャネルの輸送に関与する, 定方 哲史; 細井 延武; 柴崎 貢志; 今野 歩; 平井 宏和; 石崎 泰樹; 古市 貞一, Sep. 2019, 日本生化学会大会プログラム・講演要旨集, 92回, [1T14a, 01]

Books etc

• 遺伝子改変疾患マウスと小脳異常, Contributor, Feb. 2021, 5, 69-73, Textbook

Presentations

• Modulation of cerebellar synaptoc plasticity by thyroid hormone signaling, Nobutake HOSOI, 第99回生理学大会　「小脳の情報処理機構の新展開」シンポジウム, 16 Mar. 2022, English
• Class II ARF can be a novel Nav1.6 localizing factor at the axon initial segment of cerebellar Purkinje cells, Nobutake HOSOI, 第126回日本解剖学会　第98回日本生理学会大会　合同大会　Web開催（招待講演）, 30 Mar. 2021, 28 Mar. 2021, 30 Mar. 2021, English, Domestic conference
• Insufficiency of class II ARFs causes tremor by impairment of Nav1.6 localization to the axon initial segment of cerebellar Purkinje cells in mice, 細井延武、柴崎貢志、古市貞一、平井宏和、定方哲史, 生理研研究会　2020 シナプス研究会, 03 Dec. 2020, English, Domestic conference
• Deletion of class II ARFs causes tremor by disruption of Nav1.6 localization to the axon initial segment of cerebellar Purkinje cells in mice, Nobutake Hosoi, Koji Shibasaki, Teiichi Furuichi, Hirokazu Hirai, Tetsushi Sadakata, FENS 2020, 2020, 11 Jul. 2020, 15 Jul. 2020, English, International conference
• 小脳変性ミュータントマウス（staggerer　マウス）のシナプス伝達障害 　平行線維－プルキンエ細胞間のシナプスについて, 細井　延武, 16 Nov. 2010
• Impairment of mGluR signaling at cerebellar parallel fiber-Purkinje cell synapses in staggerer mutant mice, Hosoi N,Mitsumura K,Hirai H, Neuroscience2011, 16 Sep. 2011, Yokohama
• Disruption of mGluR-mediated signaling at cerebellar parallel fiber-Purkinje cell synapses in staggerer mutant mice, Nobutake Hosoi, Kazuhiro Mitsumura, Hirokazu Hirai, Fourth International Congress of the Society for Research on the Cerebellum, 18 Sep. 2011, Tokyo
• Disruption of mGluR-mediated signaling at cerebellar parallel fiber-Purkinje cell synapses in staggerer mutant mice, Hosoi N,Mitsumura K,Hirai H, The International Society for Radiation Neurobiology, 03 Dec. 2011, Maebashi
• Disruption of mGluR-mediated signaling at cerebellar parallel fiber-Purkinje cell synapses in staggerer mutant mice, Hosoi N,Mitsumura K,Hirai H, The 8th FENS Forum of Neuroscience, 18 Jul. 2012, Barcelona
• Contribution of vesicle recruitment and dynamic change of release probability during short-term synaptic plasticity at the calyx of Held, Nobutake Hosoi, Takeshi Sakaba, and Erwin Neher, Neuroscience 2008, 09 Jul. 2008, Tokyo
• Probing calcium dependence of vesicle recruitment and its functional role in synaptic transmission at the calyx of Held, Nobutake Hosoi, Takeshi Sakaba and Erwin Neher, Society for Neuroscience 2007, 05 Nov. 2007, San Diego

Research Projects

• Oct. 2021, Mar. 2022
• Apr. 2021, Sep. 2021
• 01 Apr. 2024, 31 Mar. 2027, 24K10022

  対象リソースIRI
• Development of microglia-targeting AAV vectors and its application to neurodegenerative disorders, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Gunma University, 01 Apr. 2023, 31 Mar. 2026, 23H02791

  対象リソースIRI
• 01 Apr. 2020, 31 Mar. 2023, 20K06906

  対象リソースIRI
• Epigenetic modulation by chemical substances on thyroid hormone-mediated brain development., Koibuchi Noriyuki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Gunma University, 01 Apr. 2018, 31 Mar. 2022, The aim of this study was to investigate the effect of environmental chemicals on the thyroid hormone (TH)-mediated signal transduction using various levels of experiment starting from in silico docking analysis to behavioral analysis. The hydroxylated PCB, which was originally to be used, became unavailable due to restriction in Japan. Instead, isoflavones, PFOS, gadolinium contrast agents, etc. were used. In isoflavones, genistein, daidzein and its metabolite equol exerted their action by crosstalk with several receptors such as TH and estrogen receptors and promoted neurite development and astrocyte migration to affect brain development. For PFOS, its exposure caused various abnormal behaviors and suppressed long-term depression of Purkinje cells, which are target cells for TH. Furthermore, the gadolinium contrast agents also acted by crosstalk of multiple receptors. In particular, the signal transduction of integrin αvβ3, which is a membrane receptor for TH, was disturbed., 18H03379

  対象リソースIRI
• Mechanism of activity-dependent motor improvement in SCA, Hosoi Nobutake, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Gunma University, 01 Apr. 2017, 31 Mar. 2022, In this study, I focused on congenital spinocerebellar ataxia (SCA) which is characterized by cerebellar dysfunction, and the purpose of the study was examining the mechanism by which oral administration of low-dose baclofen may improve motor performance of SCA model mice in an activity-dependent manner. To this end, I tried to develop a technique for activity-dependent labeling of cerebellar neurons, but it turned out to be difficult because of the unstableness and the low efficiency of activity-dependent labeling in the cerebellum. The effect of low-dose baclofen on cerebellar function was examined, and the results suggest that baclofen might act on the brain regions other than the cerebellum to improve motor performance of SCA3 model mice., 17K07103

  対象リソースIRI
• Development of gene therapy against neurodegenerative diseases using CRISPR/Cas9-based genome editing, Hirai Hirokazu; HOSOI Nobutake; KONNO Ayumu; MATSUZAKI Yasunori, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Gunma University, 01 Apr. 2016, 31 Mar. 2018, In this research, we aimed to establish a technique that allows elimination of a disease-causing gene from specific cell populations in the brain, using adeno-associated virus (AAV) vectors in combination with CRISPR/Cas9 system. Initially, we characterized neuron-specific, astrocyte-specific and cerebellar Purkinje cell-specific promoters available for AAV vector-mediated transgene expression. The results were published in 3 papers. Then, using the AAV vectors, we examined whether a gene responsible for developmental defects of the brain could be knocked out from Purkinje cells by CRISPR/Cas9 system. The results showed successful removal of the gene from around two thirds of Purkinje cells., 16K15477

  対象リソースIRI
• Elucidation of cellular and nuclear signals that regulate motor learning, Hirai Hirokazu; HOSOI Nobutake; KONNO Ayumu; MATSUZAKI Yasunori, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Gunma University, 01 Apr. 2015, 31 Mar. 2018, Decrease of several genes including transient receptor potential cation channel type 3 (TRPC3), inositol trisphosphate (IP3) receptor and Homer protein homolog 3 (HOMER3) were found in the cerebellum of spinocerebellar ataxia type 1 (SCA1) knock-in mouse. We identified retinoid-related orphan receptor α (RORα), which is a transcription factor that could regulate expression of those genes. Moreover, we found that a gene “X” is increased more than 3 times in SCA1 knock-in mice 3 h after treatment with baclofen and rotarod training. Since haploinsufficiency of the gene “X” is known to cause mental retardation, upregulation of the gene “X” may play a key role in baclofen-induced improvement of motor learning in SCA1 mice., 15H04254

  対象リソースIRI
• Functional roles of glial ATP receptors in neuron-glia interaction, HOSOI Nobutake; KONNO Ayumu; HIRAI Hirokazu, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Gunma University, 01 Apr. 2013, 31 Mar. 2017, Similarly to neurons, glial cells have ATP receptors and may play an active role in neuronal information processing. Originally, in order to examine functional roles of cerebellar glial ATP receptors, I tried to knockdown glial ATP receptors using microRNA-mediated gene silencing technique and it did not work. However, while I tried to express transgenes selectively in cerebellar glial cells with adeno-associated virus (AAV) vectors, I managed to develop a method in which larger transgenes can be expressed in glial cells, compared to the usual usage of AAV vectors. The pros and cons of this method were examined experimentally, and I am preparing for publishing the results., 25430003

  対象リソースIRI
• Neuron-glia interaction during cerebellar development The role of Ca-permeable AMPA receptors, HOSOI Nobutake, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Gunma University, 2010, 2011, Cerebellar Bergmann glial cells have Ca-permeable AMPA receptors and can be involved in formation and development of neuronal circuit via neuron-glia interactions mediated by Ca. To elucidate the functional role of Ca-permeable AMPA receptors in cerebellar glial cells, I attempted to develop a method in which glial Ca-permeable AMPA receptors are turned Ca-impermeable during cerebellar development. Using lentiviral vectors with truncated GFAP promoters, I succeeded in expressing a GluR2 gene in glial cells effectively, with no change in tropism., 22700431

  対象リソースIRI

Academic Contribution

• Competition etc, Mar. 2022, Mar. 2022, International academic contribution